BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0051.p (232 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 115 1e-25 VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pa... 96 5e-20 fliH [NU] COG1317 Flagellar biosynthesis/type III secretory path... 91 2e-18 ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pat... 88 2e-17 ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 88 2e-17 >RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Rso Ralstonia solanacearum Length = 248 Score = 115 bits (287), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 59/180 (32%), Positives = 103/180 (57%), Gaps = 12/180 (6%) Query: 52 EVDAIREQARQEGYAEGQQQGLQAGRAEGYMAGLQQGQTEVNETIQHLRQIAVSFGTEVS 111 E+ R++ R++G+A+G Q G + GR +G ++ ++ +Q +R+ + Sbjct: 63 ELANARDEGRRDGFAQGFQDGFEQGRRQG-----EEDSRQIAALMQSVREA-------ID 110 Query: 112 QTSETMAPELLNLGLDISKAMIKTALNVKPELILPTISAAIHSLPGLQLPALLYLQPDDA 171 Q + ++A EL+ L L +++ ++ AL +PE ILP + + P PA+L + DDA Sbjct: 111 QMNGSVAEELVGLALQLAQQFLRGALRAQPERILPLVREVLGDAPTAPAPAMLRVHADDA 170 Query: 172 ALVREHLGEELSRNGWRLVEDPELQRGGCRMETGTNQVDATVQTRWRRIAESLSKQLDWL 231 L+R+ LG EL+ GW LV D ++RGGCR++T + DAT+QTRW + +L + W+ Sbjct: 171 ELIRQALGTELAAAGWTLVVDAAIERGGCRVQTRFGETDATLQTRWAELTRALGRDTAWI 230 >VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Vch Vibrio cholerae Length = 270 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 65/241 (26%), Positives = 119/241 (49%), Gaps = 30/241 (12%) Query: 2 ASRNNIIPKEQMTPYQRWEMASFDEPQEEIPPPEEQFAEEIPPPPSLTLEEVDAIREQAR 61 + +N K+ Y + +FDEP++ + E +F+EE E+ IR A+ Sbjct: 34 GAESNKAAKQTAFNYDPGWIPNFDEPEQVV---EHEFSEE----------EIALIRTAAQ 80 Query: 62 QEGY----AEGQQQGLQAGRAEGYMAG------------LQQGQTEVNETIQHLRQIAVS 105 QEG+ AEG QQG + G+AEG+ AG + +GQ + E ++ +A Sbjct: 81 QEGFEAGQAEGYQQGFEQGKAEGFQAGHQEGQTQGYQDGVAEGQALIQEQVKTFMALANQ 140 Query: 106 FGTEVSQTSETMAPELLNLGLDISKAMIKTALNVKPELILPTISAAIHSLPGLQLPALLY 165 F + + + +L+++ L ++K ++ + P++IL T+ A++ +LP L Sbjct: 141 FAQPLDLLNAQVEKQLVDMVLALTKEVVHVEVQTNPQVILDTVKASVEALPIAGHAITLK 200 Query: 166 LQPDDAALVREHLGE-ELSRNGWRLVEDPELQRGGCRMETGTNQVDATVQTRWRRIAESL 224 L P+D ++R+ GE E+ W L+ +P L RG ++E G + V ++ R R + +S Sbjct: 201 LNPEDVEIIRQAYGEQEIETRNWTLLSEPALSRGDVQIEAGESSVSYRMEERIRSVLKSF 260 Query: 225 S 225 Sbjct: 261 C 261 >fliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Eco Escherichia coli K12 Length = 235 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 67/222 (30%), Positives = 96/222 (43%), Gaps = 8/222 (3%) Query: 8 IPKEQMTPYQRWEMASFDEPQEEIPP---PEEQFAEEIPPP-PSLTLEEVDAIREQARQE 63 IP P++ W PQ E P PEE EE P + EQ Q Sbjct: 6 IPMSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQAHEQGYQA 65 Query: 64 GYAEGQQQGLQAGRAEGYMAGLQQGQTEVNE----TIQHLRQIAVSFGTEVSQTSETMAP 119 G AEG+QQG + G EG GL+QG E ++Q+ F T + +A Sbjct: 66 GIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIAS 125 Query: 120 ELLNLGLDISKAMIKTALNVKPELILPTISAAIHSLPGLQLPALLYLQPDDAALVREHLG 179 L+ + L+ ++ +I V ++ I + P L + PDD V + LG Sbjct: 126 RLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLG 185 Query: 180 EELSRNGWRLVEDPELQRGGCRMETGTNQVDATVQTRWRRIA 221 LS +GWRL DP L GGC++ +DA+V TRW+ + Sbjct: 186 ATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELC 227 >ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein EcZ Escherichia coli O157:H7 EDL933 Length = 235 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 66/222 (29%), Positives = 95/222 (42%), Gaps = 8/222 (3%) Query: 8 IPKEQMTPYQRWEMASFDEPQEEIPP---PEEQFAEEIPPP-PSLTLEEVDAIREQARQE 63 IP P++ W PQ E P EE EE P + EQ Q Sbjct: 6 IPMSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQLAQLQMQAHEQGYQA 65 Query: 64 GYAEGQQQGLQAGRAEGYMAGLQQGQTEVNE----TIQHLRQIAVSFGTEVSQTSETMAP 119 G AEG+QQG + G EG GL+QG E ++Q+ F T + +A Sbjct: 66 GIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIAS 125 Query: 120 ELLNLGLDISKAMIKTALNVKPELILPTISAAIHSLPGLQLPALLYLQPDDAALVREHLG 179 L+ + L+ ++ +I V ++ I + P L + PDD V + LG Sbjct: 126 RLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLG 185 Query: 180 EELSRNGWRLVEDPELQRGGCRMETGTNQVDATVQTRWRRIA 221 LS +GWRL DP L GGC++ +DA+V TRW+ + Sbjct: 186 ATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELC 227 >ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ecs Escherichia coli O157:H7 Length = 235 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 66/222 (29%), Positives = 95/222 (42%), Gaps = 8/222 (3%) Query: 8 IPKEQMTPYQRWEMASFDEPQEEIPP---PEEQFAEEIPPP-PSLTLEEVDAIREQARQE 63 IP P++ W PQ E P EE EE P + EQ Q Sbjct: 6 IPMSDNLPWKTWTPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQLAQLQMQAHEQGYQA 65 Query: 64 GYAEGQQQGLQAGRAEGYMAGLQQGQTEVNE----TIQHLRQIAVSFGTEVSQTSETMAP 119 G AEG+QQG + G EG GL+QG E ++Q+ F T + +A Sbjct: 66 GIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDALDSVIAS 125 Query: 120 ELLNLGLDISKAMIKTALNVKPELILPTISAAIHSLPGLQLPALLYLQPDDAALVREHLG 179 L+ + L+ ++ +I V ++ I + P L + PDD V + LG Sbjct: 126 RLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVDDMLG 185 Query: 180 EELSRNGWRLVEDPELQRGGCRMETGTNQVDATVQTRWRRIA 221 LS +GWRL DP L GGC++ +DA+V TRW+ + Sbjct: 186 ATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQELC 227