BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0025.p (434 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 340 4e-93 AGpT161 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 332 1e-90 mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 330 5e-90 AGl2922 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 313 6e-85 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 310 3e-84 >BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 441 Score = 340 bits (871), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 179/435 (41%), Positives = 256/435 (58%), Gaps = 16/435 (3%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 I L FL H WRHP + + LDY+K +A+T ERGK D+LF AD L++ + Sbjct: 8 IKLGVFL--AGTGHHVASWRHPDAPSDASMNLDYFKELAKTAERGKLDMLFLADSLSIDS 65 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 + SH + TR +P +L A+A VTS+IGL AT STTYS+P+ +AR+F++LD Sbjct: 66 K---SHPNVL-------TRFEPFTLLSALAQVTSKIGLTATASTTYSEPFHIARQFASLD 115 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 HLS GRA WNVVTS + A NF + L H RY RA+EF+E+ LW+SW+ A + Sbjct: 116 HLSNGRAGWNVVTSSIESTALNFSGEKHLEHHLRYQRAEEFVEIVKGLWDSWEEDAFIRN 175 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 +G + D + +H ++H+G +FSVRGPLN+ +PQG PVIIQAGSS G+ AA+ AEV+ Sbjct: 176 KETGEFFDKEKMHELNHKGEYFSVRGPLNVSRTPQGQPVIIQAGSSGDGKALAAKTAEVI 235 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F Q L Q FYQ +K + +F R P I ++ + P + ++ AA+ K ++ Sbjct: 236 FTAQNHLESAQEFYQSIKEQAAEFGRDPEKIAIMPGIFPIIADTEEAAQAKYKELQDLII 295 Query: 303 PIVGLSTLSSHM-NVDFSAHALDAPIDDIQVA---GMQGLFGLVKELSSEGRLTLADIGR 358 P VGL L +++ +D SA+ LD P+ + ++ F LV+E++ +T+ ++ + Sbjct: 296 PSVGLQILQNYLGGIDLSAYPLDGPLPKLDAEASNAVKSRFKLVQEMAERDNMTIRELYK 355 Query: 359 LYAGGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRG 418 AG GT +AD M+ V + DGF I P LPEG + FVD VVP LQ RG Sbjct: 356 YVAGSRGHHIFVGTPEQLADKMQEWVDTKACDGFNIMPPLLPEGIEVFVDQVVPILQERG 415 Query: 419 QFRLDYEGDTLRSYL 433 FR +YEG TLR + Sbjct: 416 VFRKEYEGTTLREHF 430 >AGpT161 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 450 Score = 332 bits (850), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 180/433 (41%), Positives = 256/433 (59%), Gaps = 7/433 (1%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 +HLA +L AG WR P S G + YK +A+ E K D+LF AD+L++ Sbjct: 1 MHLALYLEAGTHLGG---WRLPESATSGGVDWALYKTVARRAEAAKLDMLFVADKLSIDD 57 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 +G V + + TR +PL L A+ AVT IG+G T S++Y+ PY+ AR + +D Sbjct: 58 NYGQHFADTVKY--RLVTRPEPLTTLAALGAVTDHIGIGGTISSSYASPYAAARMLANID 115 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 H+S GRAAWN+VTSV+ GEA NFG + GH+ERY RA EF +V KLW+SW+A AL+L+ Sbjct: 116 HISGGRAAWNLVTSVSDGEARNFGREQHFGHEERYLRAIEFADVMGKLWDSWEADALVLD 175 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 SG +AD H IDH G F +RGP+N+P PQG PV+IQAG S R Q AA+ AEV+ Sbjct: 176 KKSGVFADSTKFHYIDHEGDFFKIRGPINVPRPPQGRPVLIQAGVSDRFQGIAAQNAEVI 235 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F + +L R + FY+ K + + R +KVL ++P VG+++ A DK ++ Sbjct: 236 FVVHAELERARSFYKSFKEKVVEGGRSENAVKVLPGIVPVVGRTRKEALDKERELKELIL 295 Query: 303 PIVGLSTLSSHMNVDFSAHALDAPIDDI--QVAGMQGLFGLVKELSSEGRLTLADIGRLY 360 P GLS +S+ MN D + +AP DI ++ G G F V + + E ++T+A++G+ Y Sbjct: 296 PEAGLSFMSASMNFDLAQFPQNAPFPDITDKITGSVGRFQYVIKRAIEQKMTVAEVGKWY 355 Query: 361 AGGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQF 420 A + GT ++A + H AQ+ DGFVI P ++ D F++ VVP LQ RG F Sbjct: 356 AESLSFFAPVGTPEEVASQLAHWYAQRACDGFVILPPYIRRDEDLFLEGVVPVLQERGLF 415 Query: 421 RLDYEGDTLRSYL 433 R +Y G TLR L Sbjct: 416 RREYPGTTLRETL 428 >mlr9217 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 426 Score = 330 bits (845), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 177/433 (40%), Positives = 253/433 (58%), Gaps = 9/433 (2%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 + AFL+AG H +WRHP+ T FL +++ + +TLE G+FD LFFAD L+ Sbjct: 1 MKFVAFLLAGPTCHHHAMWRHPQ-TENRFLDPAFWETLGRTLEAGRFDGLFFADVLSFH- 58 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 + + G Q + +DP+P++ +A TSRIGLG T STT+ PY++AR S+LD Sbjct: 59 -----NETTMTRGGQ-MSLLDPVPLVAMMARATSRIGLGVTLSTTFQDPYAIARVLSSLD 112 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 LS GR AWN+VTS + +A FG L ERY+RA+E L+V LW W AL+L+ Sbjct: 113 VLSGGRIAWNIVTSFSDLDAQIFGEEALLPRSERYERAEEVLKVCLDLWTGWADGALVLD 172 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 SG + D V + G + RG +P SPQ PVI+QAGSS RG+DFAA AE+V Sbjct: 173 KESGDFIDEAKVRRTEIAGKYVGARGVFTVPPSPQRHPVIMQAGSSPRGRDFAASHAELV 232 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F +Q L MQ FY ++K R + R P D +L ++ P VG++ + A +K+A N +VD Sbjct: 233 FTLQHSLPDMQEFYAEMKDRVGRAGRNPDDCAILVSVDPIVGETNSLAREKQAYINDLVD 292 Query: 303 PIVGLSTLSSHMNVDFSAHALDAPIDDIQV-AGMQGLFGLVKELSSEGRLTLADIGRLYA 361 P VG+ +S H +D S + +D PI DI G +G F ++ + + LTL + + +A Sbjct: 293 PHVGIPFMSDHTGLDLSQYPVDKPIIDIPAETGSRGSFDIILQGTKANNLTLGEAAKHFA 352 Query: 362 GGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQFR 421 L PQV GT +AD ++ +G DGF++TP +P F+ F VVP LQ+RG FR Sbjct: 353 VSELCPQVVGTPETVADQLQDFFENKGCDGFIVTPTEMPGSFEAFTRSVVPILQKRGLFR 412 Query: 422 LDYEGDTLRSYLR 434 +Y G TLR ++ Sbjct: 413 KEYPGSTLRETIK 425 >AGl2922 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 436 Score = 313 bits (802), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 178/432 (41%), Positives = 241/432 (55%), Gaps = 15/432 (3%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 +HL FL A H WRHP + G D R+ + E KFD++F AD L Sbjct: 10 MHLGLFL--QGAGHHVSGWRHPDAEAGS-ENFDLLTRVTKMAEAAKFDMVFLADGLTS-- 64 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 GV R +PL +L A+A VT +IGL AT STTY +PY AR F+++D Sbjct: 65 --------GVDAHPSMIARFEPLTLLAALAMVTDKIGLAATASTTYGEPYHTARAFASID 116 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 HLS GRAAWN+VT+ A NF + HDERY A+E++ V LW+SWD A + + Sbjct: 117 HLSHGRAAWNIVTTSYARTAANFS-KSHPEHDERYAVAEEYVNVVRGLWDSWDDDAFVKD 175 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 +GRY DP+ VH +DH G +F+V+GPLNIP SPQG PV+IQAGSS GQD AAR A++V Sbjct: 176 KQAGRYVDPEKVHILDHEGKYFTVKGPLNIPRSPQGHPVLIQAGSSGPGQDLAARTADIV 235 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F Q ++ Q FY LK+R KF R PA + V+ MP +G+S A +K N D Sbjct: 236 FTAQQAISEAQAFYTSLKARVAKFGRDPASVAVMPGFMPIIGRSFEEAGEKLKELNRWTD 295 Query: 303 PIVGLSTLSSHMNVDFSAHALDAPIDDIQVAG-MQGLFGLVKELSSEGRLTLADIGRLYA 361 + L + + + LD P+ D+ ++ ++ L+ EL+ +LT+ ++ A Sbjct: 296 IKNAMPLLEERIGHSLADYDLDGPLPDLPISDQLRSRAELLTELARREKLTIRELALRVA 355 Query: 362 GGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQFR 421 G V GTA ++AD M+ ADGF + P P G ++F VVP LQ RG FR Sbjct: 356 AGRGHHIVMGTAKEVADRMQQWFENGAADGFNVMPPFFPGGLEEFNREVVPLLQERGLFR 415 Query: 422 LDYEGDTLRSYL 433 DYEG TLR +L Sbjct: 416 KDYEGSTLRDHL 427 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Sme Sinorhizobium meliloti Length = 449 Score = 310 bits (795), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 174/434 (40%), Positives = 248/434 (57%), Gaps = 12/434 (2%) Query: 3 IHLAAFLIAGNAAHSQVLWRHPRSTPGGFLTLDYYKRIAQTLERGKFDLLFFADRLAVST 62 I L AFL G WRHP + G ++++R+A+T ERG FD F AD L+V Sbjct: 5 IKLGAFLPGG--GQHIAAWRHPDAPADGATNFEFHRRLAETAERGLFDAYFLADNLSVG- 61 Query: 63 RFGGSHRYGVAHGDQDATRMDPLPVLGAIAAVTSRIGLGATRSTTYSQPYSLAREFSTLD 122 GG R G G+ +P+ + A+A +T+ +G AT STTY +PY+LAR+F++LD Sbjct: 62 -LGG--REG---GNAKIAGFEPVTLFAALAPLTTHLGFIATASTTYEEPYTLARKFASLD 115 Query: 123 HLSAGRAAWNVVTSVNQGEADNFGLRETLGHDERYDRADEFLEVTHKLWNSWDAQALLLE 182 LS GRA WNVVTS A NF H +RY+RA E +E LW+SW+ A + + Sbjct: 116 LLSNGRAGWNVVTSAGDETARNFNRETQPSHADRYERAHEHVETVKALWDSWEDDAFIRD 175 Query: 183 AGSGRYADPDHVHAIDHRGTHFSVRGPLNIPASPQGGPVIIQAGSSHRGQDFAARWAEVV 242 +GR+ D VH IDH+G HFSV+GPLN P QG PV++QAG S G+ AA AEV+ Sbjct: 176 KATGRFFDAGRVHDIDHKGKHFSVKGPLNAPRPVQGHPVVVQAGQSEDGRRLAAVSAEVI 235 Query: 243 FGIQPDLARMQRFYQDLKSRTEKFCRKPADIKVLTALMPFVGKSKAAAEDKRARHNAMVD 302 F +LA Q FY+D+K+R ++ R P + ++ + PFVG+++ A K NA++ Sbjct: 236 FTAHQNLASAQEFYRDIKARVKRAGRNPEHVLIMPGVAPFVGRTEEEARSKYEELNALIV 295 Query: 303 PIVGLSTLS--SHMNVDFSAHALDAPID-DIQVAGMQGLFGLVKELSSEGRLTLADIGRL 359 P G++ L+ + +D + + LD P+ + GM+ L+++++ E T+ + + Sbjct: 296 PEDGVALLNGLTGGTLDLTGYPLDGPLPVSNETEGMKSRQALIRKIADEHGFTIRQLYQW 355 Query: 360 YAGGVLVPQVAGTAADIADWMEHIVAQQGADGFVITPAHLPEGFDDFVDLVVPELQRRGQ 419 A V G+A +AD +E + ADGF I P LP DDFVDLV+P LQRRG Sbjct: 356 IATARGHYTVVGSAEQVADQLEEWFLSEAADGFNILPPWLPGALDDFVDLVIPILQRRGL 415 Query: 420 FRLDYEGDTLRSYL 433 FR YEG TLR L Sbjct: 416 FRTAYEGRTLRENL 429