BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0023.p (281 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZfdhD [C] COG1526 Uncharacterized protein required for formate d... 232 8e-61 ECs4821 [C] COG1526 Uncharacterized protein required for formate... 232 8e-61 fdhD [C] COG1526 Uncharacterized protein required for formate de... 232 8e-61 YPO4060 [C] COG1526 Uncharacterized protein required for formate... 231 1e-60 PM0410 [C] COG1526 Uncharacterized protein required for formate ... 230 3e-60 >ZfdhD [C] COG1526 Uncharacterized protein required for formate dehydrogenase activity EcZ Escherichia coli O157:H7 EDL933 Length = 277 Score = 232 bits (591), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 119/257 (46%), Positives = 167/257 (64%), Gaps = 2/257 (0%) Query: 25 EVEKWR-DGQREVVTDVVAEEVPIALEYNGISHAVMMATPADLEDFALGFSLTEGILADP 83 ++E WR D + D VAEEVP+AL YNGISH VMMA+P DLE FALGFSL+EGI+ P Sbjct: 20 QIELWRRDDLQHPRLDEVAEEVPVALVYNGISHVVMMASPKDLEYFALGFSLSEGIIESP 79 Query: 84 GELYDCEIVQGAEGIQVQMNVATERFIALKEKRRNLTGRTGCGLCGAETLEQAVRHPAAV 143 +++ ++V G++VQ+ +++ RF+ LKE+RR L GRTGCG+CG E L + P Sbjct: 80 RDIFGMDVVPSCNGLEVQIELSSRRFMGLKERRRALAGRTGCGVCGVEQLND-IGKPVQP 138 Query: 144 QAGALRFSADTLHAALDAMQARQELQQQTGATHAAAWLGRDGKIKLVREDVGRHNALDKL 203 F + L AL + Q + Q TG THAAAW+ G++ EDVGRH ALDKL Sbjct: 139 LPFTQTFDLNKLDDALRHLNDFQPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKL 198 Query: 204 IGALAEDKQDFSAGAVIITSRASYEMVQKAATVGIGFIAAISAPTALAIRLAKETDVTLI 263 +G +++ + + GAV+++SRASYEMVQK+A G+ + A+SA T LA+ +A+ ++TL+ Sbjct: 199 LGRRSQEGESWQQGAVLVSSRASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLV 258 Query: 264 GFVRRQGHVVYAGPHRL 280 GF + VY P RL Sbjct: 259 GFCKPGRATVYTHPQRL 275 >ECs4821 [C] COG1526 Uncharacterized protein required for formate dehydrogenase activity Ecs Escherichia coli O157:H7 Length = 277 Score = 232 bits (591), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 119/257 (46%), Positives = 167/257 (64%), Gaps = 2/257 (0%) Query: 25 EVEKWR-DGQREVVTDVVAEEVPIALEYNGISHAVMMATPADLEDFALGFSLTEGILADP 83 ++E WR D + D VAEEVP+AL YNGISH VMMA+P DLE FALGFSL+EGI+ P Sbjct: 20 QIELWRRDDLQHPRLDEVAEEVPVALVYNGISHVVMMASPKDLEYFALGFSLSEGIIESP 79 Query: 84 GELYDCEIVQGAEGIQVQMNVATERFIALKEKRRNLTGRTGCGLCGAETLEQAVRHPAAV 143 +++ ++V G++VQ+ +++ RF+ LKE+RR L GRTGCG+CG E L + P Sbjct: 80 RDIFGMDVVPSCNGLEVQIELSSRRFMGLKERRRALAGRTGCGVCGVEQLND-IGKPVQP 138 Query: 144 QAGALRFSADTLHAALDAMQARQELQQQTGATHAAAWLGRDGKIKLVREDVGRHNALDKL 203 F + L AL + Q + Q TG THAAAW+ G++ EDVGRH ALDKL Sbjct: 139 LPFTQTFDLNKLDDALRHLNDFQPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKL 198 Query: 204 IGALAEDKQDFSAGAVIITSRASYEMVQKAATVGIGFIAAISAPTALAIRLAKETDVTLI 263 +G +++ + + GAV+++SRASYEMVQK+A G+ + A+SA T LA+ +A+ ++TL+ Sbjct: 199 LGRRSQEGESWQQGAVLVSSRASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLV 258 Query: 264 GFVRRQGHVVYAGPHRL 280 GF + VY P RL Sbjct: 259 GFCKPGRATVYTHPQRL 275 >fdhD [C] COG1526 Uncharacterized protein required for formate dehydrogenase activity Eco Escherichia coli K12 Length = 277 Score = 232 bits (591), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 119/257 (46%), Positives = 167/257 (64%), Gaps = 2/257 (0%) Query: 25 EVEKWR-DGQREVVTDVVAEEVPIALEYNGISHAVMMATPADLEDFALGFSLTEGILADP 83 ++E WR D + D VAEEVP+AL YNGISH VMMA+P DLE FALGFSL+EGI+ P Sbjct: 20 QIELWRRDDLQHPRLDEVAEEVPVALVYNGISHVVMMASPKDLEYFALGFSLSEGIIESP 79 Query: 84 GELYDCEIVQGAEGIQVQMNVATERFIALKEKRRNLTGRTGCGLCGAETLEQAVRHPAAV 143 +++ ++V G++VQ+ +++ RF+ LKE+RR L GRTGCG+CG E L + P Sbjct: 80 RDIFGMDVVPSCNGLEVQIELSSRRFMGLKERRRALAGRTGCGVCGVEQLND-IGKPVQP 138 Query: 144 QAGALRFSADTLHAALDAMQARQELQQQTGATHAAAWLGRDGKIKLVREDVGRHNALDKL 203 F + L AL + Q + Q TG THAAAW+ G++ EDVGRH ALDKL Sbjct: 139 LPFTQTFDLNKLDDALRHLNDFQPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKL 198 Query: 204 IGALAEDKQDFSAGAVIITSRASYEMVQKAATVGIGFIAAISAPTALAIRLAKETDVTLI 263 +G +++ + + GAV+++SRASYEMVQK+A G+ + A+SA T LA+ +A+ ++TL+ Sbjct: 199 LGRRSQEGESWQQGAVLVSSRASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLV 258 Query: 264 GFVRRQGHVVYAGPHRL 280 GF + VY P RL Sbjct: 259 GFCKPGRATVYTHPQRL 275 >YPO4060 [C] COG1526 Uncharacterized protein required for formate dehydrogenase activity Ype Yersinia pestis Length = 274 Score = 231 bits (589), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 126/272 (46%), Positives = 173/272 (63%), Gaps = 4/272 (1%) Query: 9 PDDVSAAAEFATAAKVEVEKWRDGQREVVTDVVAEEVPIALEYNGISHAVMMATPADLED 68 P +S++AE A +++V + R E D +AEEVP+AL YNGISH VMMATP DL Sbjct: 6 PSRLSSSAEIRGARQLDVLQ-RHKLAEPQQDWLAEEVPVALVYNGISHVVMMATPKDLAA 64 Query: 69 FALGFSLTEGILADPGELYDCEIVQGAEGIQVQMNVATERFIALKEKRRNLTGRTGCGLC 128 FALGFSL+EGI++ P E+Y E+ G I+V + +++ RF LKE+RR + GRTGCG+C Sbjct: 65 FALGFSLSEGIISSPQEIYSIEMTPGCNSIEVNIELSSRRFAGLKERRRAMAGRTGCGVC 124 Query: 129 GAETLEQAVRHPAAVQAGALRFSADTLHAALDAMQARQELQQQTGATHAAAWLGRDGKIK 188 G E L+ R P F+ + L AL ++ Q + Q TG THAAAW+ +G++ Sbjct: 125 GIEQLDDIFR-PITPLPFTQAFNLEHLDTALAQLKQVQPVGQLTGCTHAAAWINPEGELL 183 Query: 189 LVREDVGRHNALDKLIGALAEDKQDFSAGAVIITSRASYEMVQKAATVGIGFIAAISAPT 248 EDVGRH ALDKL+G A KQ + GAV+++SRASYEMVQK A G + A+SA T Sbjct: 184 GGCEDVGRHVALDKLLGIRA--KQPWQQGAVLVSSRASYEMVQKTAMCGAEILFAVSAAT 241 Query: 249 ALAIRLAKETDVTLIGFVRRQGHVVYAGPHRL 280 LA+ +A+ ++TL+GF + VY P R+ Sbjct: 242 TLAVEVAERCNLTLVGFSKPGRATVYTHPQRI 273 >PM0410 [C] COG1526 Uncharacterized protein required for formate dehydrogenase activity Pmu Pasteurella multocida Length = 273 Score = 230 bits (587), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 122/264 (46%), Positives = 167/264 (63%), Gaps = 3/264 (1%) Query: 18 FATAAKVEVEKWRDGQREVVTDVVAEEVPIALEYNGISHAVMMATPADLEDFALGFSLTE 77 F K + K + E + +A EVP+AL YNGISH VMM +P +L DFA+GFSL E Sbjct: 12 FCKLTKKQQIKNKTTTYEAKQENLATEVPVALVYNGISHTVMMCSPENLADFAMGFSLAE 71 Query: 78 GILADPGELYDCEIVQGAEGIQVQMNVATERFIALKEKRRNLTGRTGCGLCGAETLEQAV 137 GI+ P ++Y ++V +GI+VQ+ +AT F+ LK +RR L GRTGCG+CG E L+Q Sbjct: 72 GIIDKPSDIYGIDVVPACQGIEVQIELATRCFVRLKAQRRTLAGRTGCGICGTEQLQQVH 131 Query: 138 RHPAAVQAGALRFSADTLHAALDAMQARQELQQQTGATHAAAWLGRDGKIKLVREDVGRH 197 ++ ++ ++F + L A L +Q Q L QQTG+THAA + G + +REDVGRH Sbjct: 132 KNLPPLER-TIQFDLNRLDACLIQLQQAQVLGQQTGSTHAAGFFDLAGNLLCLREDVGRH 190 Query: 198 NALDKLIGALAEDKQDFSAGAVIITSRASYEMVQKAATVGIGFIAAISAPTALAIRLAKE 257 ALDKL+G A KQ + G V++TSRASYEMVQK A GI + AISA T LA+ +A++ Sbjct: 191 VALDKLLGWHA--KQPAAKGFVLVTSRASYEMVQKTAACGIEMLIAISAATELAVNMAEQ 248 Query: 258 TDVTLIGFVRRQGHVVYAGPHRLI 281 +TL+GF R +Y G RL Sbjct: 249 YQLTLVGFAREGRATIYTGKERLF 272