BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0003.p (363 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0367_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) ac... 133 7e-31 Z4856 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid l... 100 9e-21 ECs4331 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 100 1e-20 RSp0367_2 [I] COG0764 3-hydroxymyristoyl/3-hydroxydecanoyl-(acyl... 75 2e-13 XF1038 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 65 2e-10 >RSp0367_1 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Rso Ralstonia solanacearum Length = 438 Score = 133 bits (334), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 89/214 (41%), Positives = 113/214 (52%), Gaps = 19/214 (8%) Query: 10 AIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMRRV 69 A+FSSGGPL AA G P EIYGS+ETGGIAWR+ E D+W+ V Sbjct: 241 AVFSSGGPLDEAAAATFASAHGTAPTEIYGSTETGGIAWRRRNE------SDAWQAFANV 294 Query: 70 EVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKRIS 129 VR ++ L VRSPHL W R D EF + A F LRGR DRIVKL+ KR+S Sbjct: 295 AVRREAD-GALSVRSPHLGHPDWHRTDDAAEF----DDAGRFRLRGRMDRIVKLDGKRVS 349 Query: 130 LDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLEQQGKLAL 189 L +IE L P A+ ++ LP +S RER+ T AG L Q G++ L Sbjct: 350 LPEIENWLGLHPYVAQSAAVL---LPGAS-----RERLGVLSALTSAGVEALRQHGRIGL 401 Query: 190 NTQLRAVLADAIESVALPRLWRYLDALPANTQGK 223 LR LA + +PR WR+ +LP + +GK Sbjct: 402 AKTLRRHLAAYVAPTLIPRKWRFRMSLPVDARGK 435 >Z4856 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases EcZ Escherichia coli O157:H7 EDL933 Length = 453 Score = 99.8 bits (247), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 72/230 (31%), Positives = 111/230 (48%), Gaps = 19/230 (8%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 +Q + S+GG L T L P EIYGS+ETG IAWR ++ W P+ Sbjct: 243 VQVLLSAGGELPWQDVQHTASWLRVWPDEIYGSTETGVIAWRYREQ-----EQRRWLPLP 297 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 ++ +E D + SP + +D+ + + D ++F+ + F+L GR RIVK+EEKR Sbjct: 298 GMQ--FQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ----FHLMGRRGRIVKIEEKR 351 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLEQQGKL 187 ISL ++E+ LLA +R E + + R+ I +V D + G Sbjct: 352 ISLQEVEQRLLA---LDGIR-----EAAAVPVTRGGRQSIGVLLVLDDEAHLQWQNGGGH 403 Query: 188 ALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTP 237 + R +L +E VA+PR WR +D +P N+ K A L L + P Sbjct: 404 SQEMXWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFHEAP 453 >ECs4331 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Ecs Escherichia coli O157:H7 Length = 453 Score = 99.8 bits (247), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 72/230 (31%), Positives = 111/230 (48%), Gaps = 19/230 (8%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 +Q + S+GG L T L P EIYGS+ETG IAWR ++ W P+ Sbjct: 243 VQVLLSAGGELPWQDVQHTASWLRVWPDEIYGSTETGVIAWRYREQ-----EQRRWLPLP 297 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 ++ +E D + SP + +D+ + + D ++F+ + F+L GR RIVK+EEKR Sbjct: 298 GMQ--FQAEGDAFRLFSPLIAEDNGMLLDDILQFSEDGQ----FHLMGRRGRIVKIEEKR 351 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVVPTDAGRAVLEQQGKL 187 ISL ++E+ LLA +R E + + R+ I +V D + G Sbjct: 352 ISLQEVEQRLLA---LDGIR-----EAAAVPVTRGGRQSIGVLLVLDDEAHLQWQNGGGH 403 Query: 188 ALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALLEQTP 237 + R +L +E VA+PR WR +D +P N+ K A L L + P Sbjct: 404 SQEMTWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFHEAP 453 >RSp0367_2 [I] COG0764 3-hydroxymyristoyl/3-hydroxydecanoyl-(acyl carrier protein) dehydratases Rso Ralstonia solanacearum Length = 125 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 40/104 (38%), Positives = 59/104 (56%) Query: 259 RDDRSVVLQLHVPPDLLYFDGHFPEAPILPGVVQVDWALALGRQYFDLPAHFRALQALKF 318 RD +L VPP+L++F GHFP PILPGVV VDW L + D R++ LKF Sbjct: 21 RDAEGDHYELRVPPELVHFRGHFPGLPILPGVVLVDWIACLAAELADSTRSIRSIDQLKF 80 Query: 319 QRVVTPGATLTLELENDTQKNLLAFRLHSAGGQHASGRILFGEA 362 V P A ++++L ++ Q+ + FR + ASG +++ EA Sbjct: 81 MAPVPPAALVSVQLTHEPQRCRVRFRARLGTRECASGALVYREA 124 >XF1038 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Xfa Xylella fastidiosa 9a5c Length = 503 Score = 65.5 bits (158), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 69/227 (30%), Positives = 102/227 (44%), Gaps = 33/227 (14%) Query: 8 LQAIFSSGGPLLPDVAAATQQLLGHRPIEIYGSSETGGIAWRQPQELAGTARDDSWRPMR 67 + I S+ PL D+A A + G E++GS+ET A R+ T + +W Sbjct: 305 VAGIVSATAPLTHDLAVAAEACFGCELREVFGSTETCTFAVRR------TVYEQAWTRFD 358 Query: 68 RVEVRLNSEHDCLEVRSPHLPDDHWLRMADRVEFTSEQNPAQGFYLRGRADRIVKLEEKR 127 V + +N+ D V + HLP + +AD V+ + F + GR ++++ KR Sbjct: 359 GVSIEVNA--DVTWVYARHLPAP--MAIADFVDVYEDGR----FEVLGRQADLLEIAGKR 410 Query: 128 ISLDQIERLLLASPLTAEVRVLVHQELPSSSSSSSRRERIAAFVV-PTDAGRAVLEQQGK 186 SL ++ R LLA P + LV L + + RIAA VV PT Sbjct: 411 ASLGELNRCLLAIPGVVD-GALVQMPLEADQTVG----RIAAVVVAPT------------ 453 Query: 187 LALNTQLRAVLADAIESVALPRLWRYLDALPANTQGKTTVAALTALL 233 ++ VL I+ + LPR R +DALP N GK AL ALL Sbjct: 454 -LHEVHIQKVLRTKIDPLFLPRRLRKVDALPRNETGKLPREALLALL 499