BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0074.p (770 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2767_1 [C] COG0281 Malic enzyme Rso Ralstonia solanacearum 679 0.0 PM0002_1 [C] COG0281 Malic enzyme Pmu Pasteurella multocida 626 e-179 YPO3034_1 [C] COG0281 Malic enzyme Ype Yersinia pestis 624 e-178 STM2472_1 [C] COG0281 Malic enzyme Sty Salmonella typhimurium LT2 607 e-173 ECs3325_1 [C] COG0281 Malic enzyme Ecs Escherichia coli O157:H7 606 e-173 >RSc2767_1 [C] COG0281 Malic enzyme Rso Ralstonia solanacearum Length = 454 Score = 679 bits (1752), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/436 (78%), Positives = 388/436 (88%) Query: 8 KEELRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADPANA 67 K + R LR+AALEYHEFPTPGKISVTPTK L+NQRDL+LAYSPGVAAACEEIVAD +N+ Sbjct: 19 KAQQRAALRKAALEYHEFPTPGKISVTPTKPLSNQRDLALAYSPGVAAACEEIVADVSNS 78 Query: 68 FKYTARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINETDPD 127 F+YTARGNLVAV++NGTAVLGLG+IGP A+KPVMEGKA LFKKFAGIDVFDIEINE DP Sbjct: 79 FRYTARGNLVAVVTNGTAVLGLGDIGPEAAKPVMEGKAGLFKKFAGIDVFDIEINEKDPQ 138 Query: 128 KLCDIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAILNG 187 KL DI+ASLEPTFGGINLEDIKAPECF++ER+LR RMKIPVFHDDQHGTAI+VGA I N Sbjct: 139 KLVDIVASLEPTFGGINLEDIKAPECFFVERELRKRMKIPVFHDDQHGTAIVVGAGITNA 198 Query: 188 LKVVGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMDPDK 247 LKVVGK IK+VKLV SGAGAAALACLDL+VDLG P ENI+VTDLAGVVY+GRTELMDPDK Sbjct: 199 LKVVGKDIKQVKLVASGAGAAALACLDLLVDLGLPRENIWVTDLAGVVYEGRTELMDPDK 258 Query: 248 ERFARETDARTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILPEDV 307 FA++TD RTLA+VI ADIFLG+SA GVLKQDMVK+MA RP++ ALANP PEI+PE Sbjct: 259 AVFAQKTDKRTLAEVIDGADIFLGLSAAGVLKQDMVKRMADRPIIFALANPNPEIMPELA 318 Query: 308 MAVRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLAQAE 367 VR D I+ TGR+DYPNQVNNVLCFP+IFRGALD GATTITREME+AAVHA+A+LA+ E Sbjct: 319 KEVRPDVIMGTGRTDYPNQVNNVLCFPFIFRGALDVGATTITREMEVAAVHAVAELARQE 378 Query: 368 QSDIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSAYAE 427 QSDIVA YG+ +LSFGPEY+IPKPFDPRL++KIA AVA+AA SGVA RPI+DM AY + Sbjct: 379 QSDIVATAYGIQDLSFGPEYLIPKPFDPRLIVKIAPAVAQAAMDSGVAQRPIEDMDAYRQ 438 Query: 428 KLEQFVYHSGTFMRPI 443 L+QFVYHSGT M+PI Sbjct: 439 HLQQFVYHSGTLMKPI 454 >PM0002_1 [C] COG0281 Malic enzyme Pmu Pasteurella multocida Length = 434 Score = 626 bits (1614), Expect = e-179, Method: Compositional matrix adjust. Identities = 317/431 (73%), Positives = 358/431 (83%), Gaps = 1/431 (0%) Query: 14 QLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADPANAFKYTAR 73 QLRQAAL++HEFPTPGKI VTPTK L QRDL+LAYSPGVA C EI ADPA +++YT+R Sbjct: 4 QLRQAALDFHEFPTPGKIEVTPTKSLATQRDLALAYSPGVAVPCLEIQADPAASYRYTSR 63 Query: 74 GNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINETDPDKLCDII 133 GNLVAVISNGTAVLGLGNIG LA KPVMEGK VLFKKFAG++VFDIEI+E DPDKL DII Sbjct: 64 GNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGVNVFDIEIDERDPDKLVDII 123 Query: 134 ASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAILNGLKVVGK 193 ASLEPTFGGINLEDIKAPECFYIE+KLR+RMKIPVFHDDQHGTAII AAILNGL++V K Sbjct: 124 ASLEPTFGGINLEDIKAPECFYIEQKLRERMKIPVFHDDQHGTAIISAAAILNGLRIVKK 183 Query: 194 SIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMDPDKERFARE 253 I +VKL+ SGAGAA++ACL+L+V LG P ENI V D GV++ GR E MD K+ +A E Sbjct: 184 DIAKVKLIASGAGAASIACLNLLVSLGLPRENIIVCDSKGVIFHGRDERMDETKKLYAIE 243 Query: 254 TDA-RTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILPEDVMAVRD 312 + RTLA+VI DADIFLG SA G L QDMVK MA PL+LALANP PEILP AVR Sbjct: 244 DNGKRTLAEVINDADIFLGCSAAGTLTQDMVKTMAANPLILALANPDPEILPPLAKAVRP 303 Query: 313 DAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLAQAEQSDIV 372 DAI+ TGRSDYPNQVNNVLCFP+IFRGALD AT I EM++AAVHAIADLA AEQS++V Sbjct: 304 DAIVCTGRSDYPNQVNNVLCFPFIFRGALDVSATAINEEMKLAAVHAIADLALAEQSEVV 363 Query: 373 AATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSAYAEKLEQF 432 + YG T LSFGPEY+IPKPFDPRL++KIA AVA+AA SGVA RPIKD AY EKL QF Sbjct: 364 TSAYGETELSFGPEYLIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIKDFDAYIEKLTQF 423 Query: 433 VYHSGTFMRPI 443 VY + FM+P+ Sbjct: 424 VYKTNLFMKPV 434 >YPO3034_1 [C] COG0281 Malic enzyme Ype Yersinia pestis Length = 434 Score = 624 bits (1609), Expect = e-178, Method: Compositional matrix adjust. Identities = 313/434 (72%), Positives = 356/434 (82%), Gaps = 1/434 (0%) Query: 11 LRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADPANAFKY 70 + +QL+Q+AL++H+FP PGKI V+PTK L QRDL+LAYSPGVAA C EI ADP A+KY Sbjct: 1 MDEQLKQSALDFHQFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIAADPLAAYKY 60 Query: 71 TARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINETDPDKLC 130 TARGNLVAVISNGTAVLGLGNIG LA KPVMEGK VLFKKF+GIDVFDIE++E DPDKL Sbjct: 61 TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFSGIDVFDIEVDELDPDKLI 120 Query: 131 DIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAILNGLKV 190 DIIASLEPTFGGINLEDIKAPECFYIE+KLR+RMKIPVFHDDQHGTAII AA+LNGL+V Sbjct: 121 DIIASLEPTFGGINLEDIKAPECFYIEKKLRERMKIPVFHDDQHGTAIICTAAVLNGLRV 180 Query: 191 VGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMDPDKERF 250 V K I +VKLVVSGAGAA++ACL+L+V LG NI D GV+YKGR M K + Sbjct: 181 VKKDISDVKLVVSGAGAASIACLNLLVALGLKQHNIIACDSKGVIYKGREANMAETKAAY 240 Query: 251 ARETDA-RTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILPEDVMA 309 A + + RTL DV+P +DIFLG S GVL +MVK MAP PL+LALANP PEILP A Sbjct: 241 AIDDNGQRTLGDVMPGSDIFLGCSGSGVLTPEMVKTMAPNPLILALANPEPEILPPLAKA 300 Query: 310 VRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLAQAEQS 369 VR DAII TGRSDYPNQVNNVLCFP+IFRGALD GATTI EM++A VHAIADLA AEQS Sbjct: 301 VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATTINEEMKLACVHAIADLALAEQS 360 Query: 370 DIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSAYAEKL 429 D+VA+ YG +LSFGPEYIIPKPFDPRL++KIA AVA+AA SGVA RPI D +AY EKL Sbjct: 361 DVVASAYGEQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMASGVATRPITDFNAYVEKL 420 Query: 430 EQFVYHSGTFMRPI 443 +FVY + FM+PI Sbjct: 421 SEFVYKTNLFMKPI 434 >STM2472_1 [C] COG0281 Malic enzyme Sty Salmonella typhimurium LT2 Length = 434 Score = 607 bits (1564), Expect = e-173, Method: Compositional matrix adjust. Identities = 302/434 (69%), Positives = 351/434 (80%), Gaps = 1/434 (0%) Query: 11 LRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADPANAFKY 70 + +QL+Q+AL++HEFP PGKI V+PTK L QRDL+LAYSPGVAA C EI DP A+KY Sbjct: 1 MDEQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLAAYKY 60 Query: 71 TARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINETDPDKLC 130 TARGNLVAVISNGTAVLGLGNIG LA KPVMEGK VLFKKFAGIDVFDIE++E DPDK Sbjct: 61 TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI 120 Query: 131 DIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAILNGLKV 190 +++A+LEPTFGGINLEDIKAPECFYIE+KLR+RM IPVFHDDQHGTAII AAILNGL+V Sbjct: 121 NVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV 180 Query: 191 VGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMDPDKERF 250 V K+I +V++VVSGAGAAA+AC++L+V LG NI V D GV+YKGR M K + Sbjct: 181 VEKNISDVRMVVSGAGAAAIACMNLLVALGMQKHNIVVCDSKGVIYKGREPNMAETKAAY 240 Query: 251 A-RETDARTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILPEDVMA 309 A ++ RTL +VI ADIFLG S VL Q+MVKKMA P++LALANP PEILP Sbjct: 241 AVDDSGKRTLDEVIDGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE 300 Query: 310 VRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLAQAEQS 369 VR DAII TGRSDYPNQVNNVLCFP+IFRGALD GAT I EM++AAV AIA+LA AEQS Sbjct: 301 VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS 360 Query: 370 DIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSAYAEKL 429 ++VA+ YG +LSFGPEYIIPKPFDPRL++KIA AVA+AA SGVA RPI D AY +KL Sbjct: 361 EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMDSGVATRPIADFDAYIDKL 420 Query: 430 EQFVYHSGTFMRPI 443 +FVY + FM+PI Sbjct: 421 TEFVYKTNLFMKPI 434 >ECs3325_1 [C] COG0281 Malic enzyme Ecs Escherichia coli O157:H7 Length = 434 Score = 606 bits (1562), Expect = e-173, Method: Compositional matrix adjust. Identities = 302/434 (69%), Positives = 348/434 (80%), Gaps = 1/434 (0%) Query: 11 LRQQLRQAALEYHEFPTPGKISVTPTKLLTNQRDLSLAYSPGVAAACEEIVADPANAFKY 70 + QL+Q+AL++HEFP PGKI V+PTK L QRDL+LAYSPGVAA C EI DP A+KY Sbjct: 1 MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVAAPCLEIEKDPLKAYKY 60 Query: 71 TARGNLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEINETDPDKLC 130 TARGNLVAVISNGTAVLGLGNIG LA KPVMEGK VLFKKFAGIDVFDIE++E DPDK Sbjct: 61 TARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFAGIDVFDIEVDELDPDKFI 120 Query: 131 DIIASLEPTFGGINLEDIKAPECFYIERKLRDRMKIPVFHDDQHGTAIIVGAAILNGLKV 190 +++A+LEPTFGGINLEDIKAPECFYIE+KLR+RM IPVFHDDQHGTAII AAILNGL+V Sbjct: 121 EVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFHDDQHGTAIISTAAILNGLRV 180 Query: 191 VGKSIKEVKLVVSGAGAAALACLDLIVDLGFPIENIYVTDLAGVVYKGRTELMDPDKERF 250 V K+I +V++VVSGAGAAA+AC++L+V LG NI V D GV+Y+GR M K + Sbjct: 181 VEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVVCDSKGVIYQGREPNMAETKAAY 240 Query: 251 ARETDA-RTLADVIPDADIFLGVSAGGVLKQDMVKKMAPRPLVLALANPTPEILPEDVMA 309 A D RTL DVI ADIFLG S VL Q+MVKKMA P++LALANP PEILP Sbjct: 241 AVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKKMARAPMILALANPEPEILPPLAKE 300 Query: 310 VRDDAIIATGRSDYPNQVNNVLCFPYIFRGALDSGATTITREMEIAAVHAIADLAQAEQS 369 VR DAII TGRSDYPNQVNNVLCFP+IFRGALD GAT I EM++AAV AIA+LA AEQS Sbjct: 301 VRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDVGATAINEEMKLAAVRAIAELAHAEQS 360 Query: 370 DIVAATYGVTNLSFGPEYIIPKPFDPRLMIKIATAVARAAETSGVAARPIKDMSAYAEKL 429 ++VA+ YG +LSFGPEYIIPKPFDPRL++KIA AVA+AA SGVA RPI D Y +KL Sbjct: 361 EVVASAYGDQDLSFGPEYIIPKPFDPRLIVKIAPAVAKAAMESGVATRPIADFDVYIDKL 420 Query: 430 EQFVYHSGTFMRPI 443 +FVY + FM+PI Sbjct: 421 TEFVYKTNLFMKPI 434