BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0170.p (517 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 306 9e-83 BS_lcfA [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 261 3e-69 BS_yngI [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 259 1e-68 MTH657 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 254 4e-67 BH3103 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 253 1e-66 >BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 513 Score = 306 bits (784), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 182/514 (35%), Positives = 282/514 (54%), Gaps = 17/514 (3%) Query: 14 IRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVE 73 + A P IA + ++Y EL + A LQ+AG+ GD +A+ L NS D++ Sbjct: 7 LEETASEKPDSIACRFKDHMMTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFII 66 Query: 74 SCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADVVAALQARFPFLKTVF 133 + F ++AG V VPI+ T E+ Y + + KAI + ++ P ++ V Sbjct: 67 AFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPKVELVI 126 Query: 134 LTE-GASKAGGKDGLRYLDLRSAAAKSLPREAEDIDQ------SSFIIYTSGTTGRAKGV 186 L + G ++ D + + + A P A +Q ++ I+YTSGTTG+ KG Sbjct: 127 LCQTGEAEPEAADPEVRMKMTTFAKILRPTSAAKQNQEPVPDDTAVILYTSGTTGKPKGA 186 Query: 187 LLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHSYALNLSVLSILAVGASEYIMDKFS 246 +LT ++ L+ A A ++E D V+ LP+ H + L + + + L GA+ I +FS Sbjct: 187 MLTHQN-LYSNANDVAGYLGMDERDNVVCALPMCHVFCLTVCMNAPLMSGATVLIEPQFS 245 Query: 247 TSETLALLKSGDFTVFPAVPTIFHYLL--EGARADPGCKFPGLRLCLSAGAIMPAPINKE 304 + L+K T+F VPT+++YL E + D F +RLC+S GA MP + Sbjct: 246 PASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKD---DFSSIRLCISGGASMPVALLTA 302 Query: 305 FEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVDPSTGLDVESGQ 364 FEE+FGV +L+GYG++E S + N R GS G + + ++VDP G ++ + Q Sbjct: 303 FEEKFGVTILEGYGLSEASPVTCFNPFDRGRKPGSIGTSILHVENKVVDP-LGRELPAHQ 361 Query: 365 EGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITGRLKELIIRGGQ 424 GELIV+GPN+MKGY++ P ET AL++GW YTGDLAR D+ GY I R K++II GG Sbjct: 362 VGELIVKGPNVMKGYYKMPMETEHALKDGWLYTGDLARRDEDGYFYIVDRKKDMIIVGGY 421 Query: 425 NIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVV-SRPGMTVVEADLLLHCKERL 483 N+ P EVEE + + V + V+G P GE +VV R G+T E D++ HC++ L Sbjct: 422 NVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKRSGVT--EEDIMQHCEKHL 479 Query: 484 SSYKLPHAVHVVAEIPRTGSGKIIRYKLRETLPS 517 + YK P A+ + +IP+ +GK++R LR+ LP Sbjct: 480 AKYKRPAAITFLDDIPKNATGKMLRRALRDILPQ 513 >BS_lcfA [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 560 Score = 261 bits (667), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 168/520 (32%), Positives = 256/520 (49%), Gaps = 26/520 (5%) Query: 18 AQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDWVESCFA 77 A R P K A +++ ++ LA LQ G+ GD VA+ LPN V S + Sbjct: 34 AARFPDKTAISFYGKKLTFHDILTDALKLAAFLQCNGLQKGDRVAVMLPNCPQTVISYYG 93 Query: 78 IVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTD---------------DKADVVAAL 122 ++ AGG+ V + TEHE+ Y++ DA I T D D+ ++ ++ Sbjct: 94 VLFAGGIVVQTNPLYTEHELEYQLRDAQVSVIITLDLLFPKAIKMKTLSIVDQI-LITSV 152 Query: 123 QARFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLPREAE-----DIDQS---SFII 174 + PF K + + D + ++ + A+ + E ID + + Sbjct: 153 KDYLPFPKNILYPLTQKQKVHIDFDKTANIHTFASCMKQEKTELLTIPKIDPEHDIAVLQ 212 Query: 175 YTSGTTGRAKGVLLTQRSMLWVCAACWAPIANLNEN-DRVLSPLPLFHSYALNLSVLSIL 233 YT GTTG KGV+LT +++L C A + ++ E ++VL +P FH Y L + + Sbjct: 213 YTGGTTGAPKGVMLTHQNILANTEMCAAWMYDVKEGAEKVLGIVPFFHVYGLTAVMNYSI 272 Query: 234 AVGASEYIMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCKFPGLRLCLSA 293 +G ++ KF ETL ++ T+FP PTI+ LL ++ CLS Sbjct: 273 KLGFEMILLPKFDPLETLKIIDKHKPTLFPGAPTIYIGLLHHPELQ-HYDLSSIKSCLSG 331 Query: 294 GAIMPAPINKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPGLATRIVD 353 A +P + ++FE+ G L++GYG++E S + N+ G GS G P P I Sbjct: 332 SAALPVEVKQKFEKVTGGKLVEGYGLSEASPVTHANFIWGKNKPGSIGCPWPSTDAAIYS 391 Query: 354 PSTGLDVESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQSGYLTITG 413 TG + GE+IV+GP +MKGY KPEETA L++GW +TGD+ D+ G+ I Sbjct: 392 EETGELAAPYEHGEIIVKGPQVMKGYWNKPEETAAVLRDGWLFTGDMGYMDEEGFFYIAD 451 Query: 414 RLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRPGMTVVEA 473 R K++II GG NI P EVEEA+ EA+ + V G P + GE FVV + G Sbjct: 452 RKKDIIIAGGYNIYPREVEEALYEHEAIQEIVVAGVPDSYRGETVKAFVVLKKGAKADTE 511 Query: 474 DLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRE 513 +L + RL+ YK+P A E+P+T GKI+R +L E Sbjct: 512 ELDAFARSRLAPYKVPKAYEFRKELPKTAVGKILRRRLLE 551 >BS_yngI [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 549 Score = 259 bits (662), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 180/543 (33%), Positives = 273/543 (50%), Gaps = 42/543 (7%) Query: 5 ELI-APVGELIRRHAQRTPRK--IAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSV 61 ELI + +G L+ + A P + + Y DR+ +Y + + A L GI GD V Sbjct: 3 ELIHSTIGRLLEQTADAYPDRDAVVYPDRNIRYTYAQFDSLCRQTAKGLMRMGIGKGDHV 62 Query: 62 AIFLPNSVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDK-----A 116 AI+ N +W+ FA + G V V ++ + HE+ Y ++ + A+ D Sbjct: 63 AIWASNISEWLAVQFATAKIGAVLVTVNTNYQAHELDYLLKQSDAAALIIMDSYRGTSYP 122 Query: 117 DVVAAL-------------QARFPFLKTVFLTEGASKAGGK--DGLRYLDLRSAAAKS-- 159 D+V +L R+PFLKT+ G K G+ + D AK+ Sbjct: 123 DIVNSLIPELQEAKPGQLKSERYPFLKTLIYI------GNKRLSGMYHWDDTEILAKTVT 176 Query: 160 ---LPREAEDIDQSSFI--IYTSGTTGRAKGVLLTQRSMLWVCAACWAPIANLNENDRVL 214 L +D+ + I YTSGTTG KGV+LT +++ AA A L DR+ Sbjct: 177 DAELEERMNSLDKDNVINMQYTSGTTGFPKGVMLTHFNVI-NNAANIAECMALTSQDRMC 235 Query: 215 SPLPLFHSYALNLSVLSILAVGASEYIMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLE 274 P+P FH + L VL+ ++VGA+ + +F L ++ TV VPT+F L Sbjct: 236 IPVPFFHCFGCVLGVLACVSVGAAMIPVQEFDPVTVLKTVEKEKCTVLHGVPTMFIAELH 295 Query: 275 GARADPGCKFPGLRLCLSAGAIMPAPINKEFEERFGVM-LLDGYGITETSTMVTLNWQHG 333 D LR + AG+ P+ + K ER G+ + YG TE S ++T + Sbjct: 296 HPDFD-AYDLSTLRTGIMAGSPCPSEVMKAVIERMGMKDITIAYGQTEASPVITQTRAND 354 Query: 334 T--RVMGSCGLPLPGLATRIVDPSTGLDVESGQEGELIVRGPNLMKGYHRKPEETAKAL- 390 + R + + G LP +IV+P T +V+ G +GEL RG ++MKGY++ + T KA+ Sbjct: 355 SFIRRVETTGRALPHTEVKIVEPGTCQEVQRGMQGELCTRGYHVMKGYYKDKDATRKAIN 414 Query: 391 QNGWYYTGDLARSDQSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAP 450 +GW +TGDLA D+ GY ITGRLK+++IRGG+NI P E+EE + AVLD VVG P Sbjct: 415 HDGWLFTGDLAVMDEDGYCRITGRLKDMLIRGGENIYPREIEEFLYQHPAVLDVQVVGVP 474 Query: 451 HQHLGEVPVVFVVSRPGMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYK 510 GE ++ + G +V +L +CK +++ +K+P V + P T SGKI +YK Sbjct: 475 DAKFGEEAAAWIKLKDGKSVSPDELKAYCKGKIARHKIPRYVIFTDDYPMTASGKIQKYK 534 Query: 511 LRE 513 LRE Sbjct: 535 LRE 537 >MTH657 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mth Methanothermobacter thermautotrophicus Length = 548 Score = 254 bits (649), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 177/535 (33%), Positives = 263/535 (49%), Gaps = 37/535 (6%) Query: 10 VGELIRRHAQRTPRK--IAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPN 67 +GE + +R K I Y DR +Y E + LA L GI GD V I+ N Sbjct: 8 IGEFFEKQVERYADKEFIVYPDRDLRFTYREFNERVNLLAKGLLSIGIGKGDHVGIWATN 67 Query: 68 SVDWVESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDDKADV--------- 118 DW+ FA + G V V ++ HE+ Y ++ + KA+ D DV Sbjct: 68 VPDWLTFLFATAKIGAVLVTVNTAYKSHELEYVMKQSDMKALAIIDGFRDVDYVQTLYEL 127 Query: 119 VAALQA---------RFPFLKTVFLTEGASKAGGKDGLRYLDLRSAAAKSLP----REAE 165 V L+ RFP L++V GA K G L L K +P R Sbjct: 128 VPELKTHERGHLRSERFPELRSVIYI-GAQKHRGMYNTNELML---LGKHVPDTELRTVM 183 Query: 166 DIDQSSFII---YTSGTTGRAKGVLLTQRSMLWVCAACWAPIANLNENDRVLSPLPLFHS 222 +++ +I YTSGTTG KGV+LT R++L E DR+ P+PLFH Sbjct: 184 STLKNTDVINMQYTSGTTGFPKGVMLTHRNILN-NGYYIGERQRFTEEDRLCLPVPLFHC 242 Query: 223 YALNLSVLSILAVGASEYIMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGC 282 + + L VL++L G + +++ F LA ++ T VPT+F D Sbjct: 243 FGIVLGVLALLTHGGTLVMIELFDPLLVLAAVEKERCTALYGVPTMFIAEFTHPMFD-MF 301 Query: 283 KFPGLRLCLSAGAIMPAPINKEFEERFGVMLLD-GYGITETSTMVTLNWQHG--TRVMGS 339 LR + AG+ P K + + YG+TE S + T + + + Sbjct: 302 DLSSLRTGIMAGSPCPIEAMKRVMNDMNMKEVTIAYGLTEASPVFTQTSVDDPIEKRVET 361 Query: 340 CGLPLPGLATRIVDPSTGLDVESGQEGELIVRGPNLMKGYHRKPEETAKAL-QNGWYYTG 398 G PLP + +IVDP TG ++ G+ GE+ RG N+MKGY++ PE TA+A+ ++GW ++G Sbjct: 362 VGTPLPHIEVKIVDPETGEELGPGEPGEICCRGYNVMKGYYKMPEMTAEAIDEDGWLHSG 421 Query: 399 DLARSDQSGYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVP 458 DLA D+ GY +I GR+K++IIRGG+NI P E+EE + V D VVG P + GE+ Sbjct: 422 DLAVMDEDGYYSIVGRIKDMIIRGGENIYPREIEEFLHTMPGVKDVQVVGIPDEKYGEIV 481 Query: 459 VVFVVSRPGMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRE 513 F++ G ++E D+ + +R++ YK+P V V E P T SGK+ ++KLRE Sbjct: 482 GAFIIREDGADILEEDVRDYAIQRIARYKVPKHVFFVDEFPLTASGKVQKFKLRE 536 >BH3103 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bha Bacillus halodurans Length = 564 Score = 253 bits (645), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 164/527 (31%), Positives = 264/527 (50%), Gaps = 29/527 (5%) Query: 12 ELIRRHAQRTPRKIAYEDRHTSVSYGELEKTTTNLAGHLQDAGIAAGDSVAIFLPNSVDW 71 +LI+ A P +A +++Y + A +LQ G+ G+ V+I LPN Sbjct: 30 DLIKEAATTYPHHVALHFLGKTMTYQLFYEQVQRFAANLQQLGLQKGERVSIMLPNCPQA 89 Query: 72 VESCFAIVRAGGVAVPISYDATEHEVAYRIEDAGCKAIFTTDD--------------KAD 117 V + + ++ AGGV V + E E+ +++ D+G + I D + Sbjct: 90 VIAYYGVLVAGGVVVQTNPLYVERELNHQLIDSGARFIVCLDVLYPRVIKSREGAPLEHV 149 Query: 118 VVAALQARFPFLKT-VFLTEGASKAGGKDGL-----RYLDL-RSAAAKSLPREAEDIDQS 170 +V A++ PF K ++ K GK G R++ + +S + P + + Sbjct: 150 IVTAIKDYLPFPKNMIYPFIQKPKPKGKIGYGSHVHRFVPMAQSGSTNPTPVRIDPKEDL 209 Query: 171 SFIIYTSGTTGRAKGVLLTQRSMLWVCAACWAPIANLNE-NDRVLSPLPLFHSYALNLSV 229 + + YT GTTG AKGV+LT +++ C + + +R L+ LP FH Y + S+ Sbjct: 210 ALLQYTGGTTGVAKGVMLTHYNLVANTIQCRKWMYKWRDGQERTLAVLPFFHVYGMTTSM 269 Query: 230 LSILAVGASEYIMDKFSTSETLALLKSGDFTVFPAVPTIFHYLLEGARADPGCK---FPG 286 L G+ +I+ +F + L ++ T+FP PT++ L+ P K Sbjct: 270 NLTLMTGSELHIVPRFDPLQVLKMIDKKKITMFPGAPTMYIALIN----HPDLKKYDLSS 325 Query: 287 LRLCLSAGAIMPAPINKEFEERFGVMLLDGYGITETSTMVTLNWQHGTRVMGSCGLPLPG 346 + C+S + +PA + + FE+ G L++GYG+TE S + N G R S G+P P Sbjct: 326 IEACISGSSSLPAEVQQTFEQLTGGRLVEGYGLTEASPVTNCNLVWGERKDQSIGVPWPD 385 Query: 347 LATRIVDPSTGLDVESGQEGELIVRGPNLMKGYHRKPEETAKALQNGWYYTGDLARSDQS 406 I TG + SG+ GE++VRGP +MKGY +PEET LQNGW YTGD+ D+ Sbjct: 386 TDVHIRSLETGESLPSGEIGEVVVRGPQVMKGYWNRPEETNATLQNGWLYTGDMGYKDEE 445 Query: 407 GYLTITGRLKELIIRGGQNIAPAEVEEAVSAFEAVLDCAVVGAPHQHLGEVPVVFVVSRP 466 GY I R K++II GG NI P EVEE + A V + V+G P ++ GE F+V + Sbjct: 446 GYFYIVDRKKDMIIAGGFNIYPREVEEVLYAHAKVQEAVVIGVPDEYRGETVKAFIVVKE 505 Query: 467 GMTVVEADLLLHCKERLSSYKLPHAVHVVAEIPRTGSGKIIRYKLRE 513 G +V E +L +C+ L++YK+P ++P+T GK+++ L E Sbjct: 506 GESVSEKELNDYCRTHLAAYKVPRKYEFRDDLPKTMVGKVLKRVLIE 552