BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0129.p (285 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2005 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 209 4e-54 PAE2688 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 150 3e-36 BH2000 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 148 1e-35 MK1002 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 148 1e-35 PH0643 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 147 2e-35 >BH2005 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Bha Bacillus halodurans Length = 276 Score = 209 bits (533), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 119/287 (41%), Positives = 171/287 (59%), Gaps = 19/287 (6%) Query: 1 MKICRFDDNRLGLVEGDVVRDVTVALEILPAHTYPFPRYDLLVANLPALVDRINALKSDA 60 MK+ F+D +LGLV+ +++ D+++ +LP T P LL+ + I Sbjct: 1 MKLALFNDFQLGLVKEEMIYDISL---LLPNPTGGCP-MTLLIEHFDDYKTTIEQEYHRQ 56 Query: 61 EVLPLSSVRLLSPVANPGKVIAAPVNYQKHLKEVLDDPNLHHDNQINHIERA-GLFLKAT 119 L+ VRL P++ PGK++AAPVNY H KE+ ++H R G FLKAT Sbjct: 57 PSYRLNDVRLRQPLSRPGKIVAAPVNYMSHKKEM----------NVDHTARVLGFFLKAT 106 Query: 120 SSVVGASEGVALRKLDRRNDHEVELAVVIGKRAANVSRADALNYVAGYCIGLDITIRGP- 178 +S++G ++ + L +RR DHE+ELA VIGKRA +V + +A +Y+ GY D+T+R Sbjct: 107 TSIIGPNDPIILPYNNRRIDHELELAFVIGKRAKHVKKENAHDYIFGYTGLNDVTVRPTK 166 Query: 179 ---EERSFRKSPDTYTVFGPWLVTADEITEPGKLDFSIAVNGEIRQQSNTADLILGVEQL 235 EER RKS DT+T GPW+VT+DEI +P L S+ VN E+RQ NT ++ G+ +L Sbjct: 167 NREEERCLRKSFDTFTPIGPWIVTSDEIKDPQNLTISLQVNHEVRQHVNTNQMVCGIAEL 226 Query: 236 IEFASGFYTLEPGDIIITGTPEGVASIKPGDKMLARMEGIGEMAVAV 282 +E S TLEPGD+I TGTP+GV IK GD + +ME IG + V Sbjct: 227 VEIFSHVMTLEPGDLIATGTPDGVGPIKDGDTVTLQMESIGSFSNPV 273 >PAE2688 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Pya Pyrobaculum aerophilum Length = 306 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 110/304 (36%), Positives = 159/304 (52%), Gaps = 38/304 (12%) Query: 1 MKICRF---DDNRLGLVEGDVVRDVTVA-LEILPAHTYPFPRYD---LLVANLPAL--VD 51 MK+ F + R+G+++GDV+ D+ A L + A P YD L+ PAL VD Sbjct: 1 MKLLTFRKGEVRRVGVLQGDVIIDLPKAYLAVYDAFEAPDFLYDMKKLIAVGRPALEIVD 60 Query: 52 RINALKSDAEVLPLSSVRLLSPVANPGKVIAAPVNYQKHLKEVLDDPNLHHDNQINHIER 111 + + L + PV NP K+ A VNY+ H +E P ER Sbjct: 61 YLARKAPEEAKLSPKEIVWEPPVTNPEKIFAVAVNYKAHGQEAGVKPP----------ER 110 Query: 112 AGLFLKATSSVVGASEGVALRKLDRRNDHEVELAVVIGKRAANVSRADALNYVAGYCIGL 171 F K +++VG + V K+ ++ D EVEL VVIG+ + A AL+YV GY +G Sbjct: 111 PYFFPKFPNALVGHEQPVIKHKVTQKVDWEVELVVVIGRAGKYIDPARALDYVFGYTVGN 170 Query: 172 DITIR-------GPEERS-------FRKSPDTYTVFGPWLVTADEITEPGKLDFSIAVNG 217 DI+IR P++ + + KS DT GP++VT DEI +P +L + VNG Sbjct: 171 DISIRDWQFPPGWPQQLNPYGQNWIWGKSMDTAAPVGPYIVTKDEIEDPNRLGLRLRVNG 230 Query: 218 EIRQQSNTADLILGVEQLIEFASGFYTLEPGDIIITGTPEGVA-----SIKPGDKMLARM 272 ++ Q+ NT++LI V+QLI +AS TL+PGD+I TGTP GV +K GD + A + Sbjct: 231 QLEQEGNTSELIFNVQQLIHWASQGITLKPGDLIFTGTPPGVGFPKGKFLKHGDVVEAEV 290 Query: 273 EGIG 276 E IG Sbjct: 291 EKIG 294 >BH2000 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Bha Bacillus halodurans Length = 319 Score = 148 bits (374), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 103/319 (32%), Positives = 165/319 (51%), Gaps = 45/319 (14%) Query: 1 MKICRF---DDNRLGLVEGDVVRDVT-----------------VALEILPAHTYPF---P 37 MKI + D R+G V G+ V D+ +A ++PA++ F Sbjct: 1 MKIINYRSKDHVRIGCVIGNYVIDLNRAYAAMLEAQGLAKANEIADAVVPANSLSFLEGG 60 Query: 38 RYDLLVANLPALVDRINALKSD-AEVLPLSSVRLLSPVANPGKVIAAPVNYQKHLKEVLD 96 + L A+ + N L D ++V V+ +P+ N K+I NY++H+ E+ Sbjct: 61 EHTFLEASKAVQSIQKNELTPDQSKVFHEREVKKEAPILNSKKIICVGHNYREHILEMKR 120 Query: 97 DPNLHHDNQINHIERAGLFLKATSSVVGASEGVALRKLDRRNDHEVELAVVIGKRAANVS 156 + + +F K ++++G + V L + + D+E ELA VIGK+A +V+ Sbjct: 121 EIPKY----------PVIFAKFANTIIGPEDNVPLPSITEQLDYEAELAFVIGKKAKDVA 170 Query: 157 RADALNYVAGYCIGLDITIRGPEERSFR----KSPDTYTVFGPWLVTADEITEPGKLDFS 212 + DAL+YVAGY I DIT R + R+ + K+ D GPWLVT DEI P +L+ + Sbjct: 171 KEDALDYVAGYTIVNDITARDLQRRTIQWLQGKTLDGSLPMGPWLVTKDEIPNPHQLEIT 230 Query: 213 IAVNGEIRQQSNTADLILGVEQLIEFASGFYTLEPGDIIITGTPEGVAS-------IKPG 265 ++VNGE RQ+SNT +L+ ++ L+ F SG TLEPGD++ TGTP GV ++ G Sbjct: 231 LSVNGEERQRSNTKNLVFSIQDLVVFLSGIMTLEPGDVVCTGTPGGVGVAREPQLFLQDG 290 Query: 266 DKMLARMEGIGEMAVAVRA 284 D + +E +G + V+ Sbjct: 291 DVVRIELEKVGTLENRVKT 309 >MK1002 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mka Methanopyrus kandleri AV19 Length = 264 Score = 148 bits (374), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 92/244 (37%), Positives = 136/244 (55%), Gaps = 14/244 (5%) Query: 39 YDLLVANLPALVDRINALKSDAEVLPLSSVRLLSPVANPGKVIAAPVNYQKHLKEVLDDP 98 YDL V +V D V PLS +R+ PV P K+I +NY++H++E+ Sbjct: 25 YDLPVLEFIEVVHSDGLGDLDYSVYPLSELRIGPPVPRPPKIICFGLNYREHVEEL---- 80 Query: 99 NLHHDNQINHIERAGLFLKATSSVVGASEGVALRKLDRRNDHEVELAVVIGKRAANVSRA 158 + ++ + +KA ++V+G + V L + RR DHE+ELAVVIG+R VS Sbjct: 81 ---REMGMDVPSEPVMTMKAPTAVIGHLDTVKLPREARRVDHELELAVVIGERCRKVSPE 137 Query: 159 DALNYVAGYCIGLDITIRGPEERSFR----KSPDTYTVFGPWLVTADEITEPGKLDFSIA 214 +A + V G+ I D+T R E+R + KS DT+ GPW+ T EP L+ + Sbjct: 138 EARDAVLGFTIINDVTARDIEKREGQWVRAKSYDTFAPLGPWIETE---LEPDGLEMELR 194 Query: 215 VNGEIRQQSNTADLILGVEQLIEFASGFYTLEPGDIIITGTPEGVASIKPGDKMLARMEG 274 VNGE+RQ++ T D++ +L+ F S TLEPGDII TGTP GV ++P DK+ +E Sbjct: 195 VNGEVRQRATTDDMVRDPYELVSFTSRVMTLEPGDIIATGTPPGVGPMEPDDKIELEIER 254 Query: 275 IGEM 278 IG + Sbjct: 255 IGRL 258 >PH0643 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Pho Pyrococcus horikoshii Length = 230 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 91/234 (38%), Positives = 133/234 (56%), Gaps = 27/234 (11%) Query: 65 LSSVRLLSPVAN------PGKVIAAPVNYQKHLKEVLDDPNLHHDNQINHIERAGLFLKA 118 +SSVRL P + P K++A NY +H +E+ +P E +FLK Sbjct: 4 MSSVRL--PFRDGYYDVRPTKIVALAKNYAEHAREMGSEPP----------EEPIIFLKP 51 Query: 119 TSSVVGASEGVALRKLDRRNDHEVELAVVIGKRAANVSRADALNYVAGYCIGLDITIRGP 178 S+++G + L + +R DHEVELAV++GKRA NV + A +Y+ GY I LDIT R Sbjct: 52 PSALIGPGSSIILPRRSKRVDHEVELAVIMGKRAKNVPASKAFDYILGYTIILDITARDL 111 Query: 179 EERSFRKSP--------DTYTVFGPWLVTADEITEPGKLDFSIAVNGEIRQQSNTADLIL 230 + + +K DT+ GP +V + E+ +P L+ + VNG+IRQ T+ +I Sbjct: 112 QAEARKKGYPWTISKGFDTFAPIGPRVVDSREL-DPSDLEIGLKVNGKIRQLGRTSQMIF 170 Query: 231 GVEQLIEFASGFYTLEPGDIIITGTPEGVASIKPGDKMLARMEGIGEMAVAVRA 284 + +LIE+ S TLEPGDII TGTP GV ++ GD++ A +EGIG+M V A Sbjct: 171 KIPELIEYISHIMTLEPGDIIATGTPPGVGPLRHGDRIEAWIEGIGKMEFDVLA 224