BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0116.p (380 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1087 Non COG 458 e-128 PA2470 [Q] COG3435 Gentisate 1,2-dioxygenase Pae Pseudomonas aer... 414 e-115 STM2178 [Q] COG3435 Gentisate 1,2-dioxygenase Sty Salmonella typ... 405 e-112 Z3393 [Q] COG3435 Gentisate 1,2-dioxygenase EcZ Escherichia coli... 401 e-111 ECs3030 [Q] COG3435 Gentisate 1,2-dioxygenase Ecs Escherichia co... 401 e-111 >RSc1087 Non COG Length = 348 Score = 458 bits (1178), Expect = e-128, Method: Compositional matrix adjust. Identities = 223/337 (66%), Positives = 262/337 (77%) Query: 33 ERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEVITAEEAV 92 ER Y L + LAPLWESLH LVP++P PR VPA W+Y I +M+AG +I+AEEAV Sbjct: 9 ERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGRIISAEEAV 68 Query: 93 RRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGAYTTVNGE 152 RRVLVLENPGLPG SSIT +LYAGLQLILP E+AP+HRH+QSALRFIV+G+GA+T V+GE Sbjct: 69 RRVLVLENPGLPGRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKGAWTAVDGE 128 Query: 153 RSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAENNSSASQI 212 R+TMHPGDFIITPSWTWHDHG P + EPVVWLDGLDIP+V+ FDAGFAEN+ A Q Sbjct: 129 RTTMHPGDFIITPSWTWHDHGCPRLDEGGEPVVWLDGLDIPLVQQFDAGFAENHPEAQQP 188 Query: 213 PTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDEWHGIKLR 272 T+ EG SFA +G+NM PVR ++ SP+ +YPY RSREALDTL+R LD W G+KLR Sbjct: 189 VTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELDPWDGVKLR 248 Query: 273 YVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQSFSFSPKD 332 YVNP TGG+PMPTIA F+Q LP GF+GK +RS+D TVY VVEG G I + F+F+P D Sbjct: 249 YVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEARFAFAPHD 308 Query: 333 TFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLRE 369 FVVPSWAPV D D VLFS+SDRPVL A LLRE Sbjct: 309 VFVVPSWAPVRLDAQDDAVLFSYSDRPVLAALNLLRE 345 >PA2470 [Q] COG3435 Gentisate 1,2-dioxygenase Pae Pseudomonas aeruginosa Length = 353 Score = 414 bits (1063), Expect = e-115, Method: Compositional matrix adjust. Identities = 212/346 (61%), Positives = 253/346 (73%), Gaps = 2/346 (0%) Query: 26 MKIDQTAERQA-LYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGE 84 M D + E++A Y ++++ +L PLWE+LH+LVP QP ALWRY ++ L+ AG+ Sbjct: 1 MHNDNSTEKRADFYRRIRQQHLTPLWEALHSLVPAQPAGGCQAALWRYRELRPFLLEAGD 60 Query: 85 VITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQG 144 +I+AEEAVRRVLVLENP LPG SSIT SLYAGLQLILP E+AP+HRH+QSALRF+V+G G Sbjct: 61 LISAEEAVRRVLVLENPDLPGQSSITPSLYAGLQLILPGEIAPSHRHTQSALRFVVEGYG 120 Query: 145 AYTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAE 204 AYT+V+GER+ M PGDFIITPSWTWHDHGN A EPVVWLDGLDIPM+RFF A FAE Sbjct: 121 AYTSVDGERTRMEPGDFIITPSWTWHDHGNRAAAEGGEPVVWLDGLDIPMLRFFGATFAE 180 Query: 205 NNSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLD 264 N+ Q + EG S A YG NM P+R T SP+ +YPYARSREAL+ L R D Sbjct: 181 NHDEPVQPLRRGEGDSLARYGSNMLPLRHQPTTPTSPLFSYPYARSREALERLARLERPD 240 Query: 265 EWHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQ 324 W G KLRYVNP TGG+ MPTIA LQLLP GF G+ RS+D +VY VVEG G QI + Sbjct: 241 PWEGHKLRYVNPATGGWAMPTIATCLQLLPAGFLGQPARSTDASVYSVVEGSGVAQIGGR 300 Query: 325 SFSFSPKDTFVVPSWAPVCFDTPA-DTVLFSFSDRPVLEATGLLRE 369 F+F KD FVVPSWA + + A D VLFSFSDRPV +A G+LRE Sbjct: 301 RFAFEAKDLFVVPSWAELRLEAGATDCVLFSFSDRPVQQALGILRE 346 >STM2178 [Q] COG3435 Gentisate 1,2-dioxygenase Sty Salmonella typhimurium LT2 Length = 345 Score = 405 bits (1040), Expect = e-112, Method: Compositional matrix adjust. Identities = 206/348 (59%), Positives = 242/348 (69%), Gaps = 7/348 (2%) Query: 27 KIDQTAE--RQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGE 84 +I+Q + RQ Y + NL PLWESLH LVP+ P PA W Y +I LM +G Sbjct: 3 EINQNVKDSRQQYYQHISGQNLTPLWESLHHLVPQTPNANCAPAYWNYQEIRPLLMESGN 62 Query: 85 VITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQG 144 VI A+EA+RRVLVLENP L G SSIT +LYAGLQLILP EVAP+HRH+QSALRFIV+G+G Sbjct: 63 VIGAKEAIRRVLVLENPALRGQSSITATLYAGLQLILPGEVAPSHRHNQSALRFIVEGKG 122 Query: 145 AYTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAE 204 A+T V+GER+ MH GDFI+TP W WHDHGNPG SEPVVWLDGLD+P+V GFAE Sbjct: 123 AFTAVDGERTPMHTGDFILTPQWRWHDHGNPG----SEPVVWLDGLDLPLVNLLGCGFAE 178 Query: 205 NNSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLD 264 + Q T+ EG Y NM P+R SPI NY Y RSREAL L R D Sbjct: 179 DYPEDQQPVTRKEGDYLPRYAANMLPLRH-QRGNSSPIFNYRYDRSREALHDLTRMGDPD 237 Query: 265 EWHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQ 324 EW G KLRYVNPVTGGYPMP++ AFLQLLPKGF + RS+D T+YHVVEG G+V I ++ Sbjct: 238 EWEGYKLRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARSTDSTIYHVVEGAGQVTIGNE 297 Query: 325 SFSFSPKDTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLREEYF 372 +F FS KD FV P+W V F + DTVLFSFSD+PV EA GL RE + Sbjct: 298 TFHFSAKDIFVAPTWHEVSFRSSEDTVLFSFSDKPVQEALGLFREARY 345 >Z3393 [Q] COG3435 Gentisate 1,2-dioxygenase EcZ Escherichia coli O157:H7 EDL933 Length = 342 Score = 401 bits (1031), Expect = e-111, Method: Compositional matrix adjust. Identities = 201/347 (57%), Positives = 240/347 (69%), Gaps = 5/347 (1%) Query: 26 MKIDQTAERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEV 85 M + R+ Y + NL PLWESLH LVPK P PA W Y +I L+ +G + Sbjct: 1 MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAYWNYQEIRPLLLESGGL 60 Query: 86 ITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGA 145 I A+EAVRRVLVLENP L G SSIT +LYAGLQLI+P EVAP+HRH+QSALRFIV+G+GA Sbjct: 61 IGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAPSHRHNQSALRFIVEGKGA 120 Query: 146 YTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAEN 205 +T V+GER+ M+ GDFI+TP W WHDHGNPG EPV+WLDGLD+P+V GFAE+ Sbjct: 121 FTAVDGERTPMNEGDFILTPQWRWHDHGNPG----DEPVIWLDGLDLPLVNILGCGFAED 176 Query: 206 NSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDE 265 Q T+ EG Y NM P+R T SPI NY Y RSRE L L R DE Sbjct: 177 YPEEQQPVTRKEGDYLPRYAANMLPLRH-QTGNSSPIFNYRYDRSREVLHDLTRLGDADE 235 Query: 266 WHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQS 325 W G K+RYVNPVTGGYPMP++ AFLQLLPKGF + R++D T+YHVVEG G+V I +++ Sbjct: 236 WDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTDSTIYHVVEGSGQVIIGNET 295 Query: 326 FSFSPKDTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLREEYF 372 FSFS KD FVVP+W V F T D+VLFSFSDRPV EA GL RE + Sbjct: 296 FSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEALGLFREARY 342 >ECs3030 [Q] COG3435 Gentisate 1,2-dioxygenase Ecs Escherichia coli O157:H7 Length = 342 Score = 401 bits (1031), Expect = e-111, Method: Compositional matrix adjust. Identities = 201/347 (57%), Positives = 240/347 (69%), Gaps = 5/347 (1%) Query: 26 MKIDQTAERQALYGKLQELNLAPLWESLHTLVPKQPLPRYVPALWRYTDIHSHLMRAGEV 85 M + R+ Y + NL PLWESLH LVPK P PA W Y +I L+ +G + Sbjct: 1 MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAYWNYQEIRPLLLESGGL 60 Query: 86 ITAEEAVRRVLVLENPGLPGNSSITQSLYAGLQLILPDEVAPAHRHSQSALRFIVDGQGA 145 I A+EAVRRVLVLENP L G SSIT +LYAGLQLI+P EVAP+HRH+QSALRFIV+G+GA Sbjct: 61 IGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAPSHRHNQSALRFIVEGKGA 120 Query: 146 YTTVNGERSTMHPGDFIITPSWTWHDHGNPGIAGVSEPVVWLDGLDIPMVRFFDAGFAEN 205 +T V+GER+ M+ GDFI+TP W WHDHGNPG EPV+WLDGLD+P+V GFAE+ Sbjct: 121 FTAVDGERTPMNEGDFILTPQWRWHDHGNPG----DEPVIWLDGLDLPLVNILGCGFAED 176 Query: 206 NSSASQIPTQVEGRSFANYGYNMAPVRQLHTERHSPISNYPYARSREALDTLFRSAVLDE 265 Q T+ EG Y NM P+R T SPI NY Y RSRE L L R DE Sbjct: 177 YPEEQQPVTRKEGDYLPRYAANMLPLRH-QTGNSSPIFNYRYDRSREVLHDLTRLGDADE 235 Query: 266 WHGIKLRYVNPVTGGYPMPTIAAFLQLLPKGFQGKWHRSSDGTVYHVVEGHGRVQIADQS 325 W G K+RYVNPVTGGYPMP++ AFLQLLPKGF + R++D T+YHVVEG G+V I +++ Sbjct: 236 WDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTDSTIYHVVEGSGQVIIGNET 295 Query: 326 FSFSPKDTFVVPSWAPVCFDTPADTVLFSFSDRPVLEATGLLREEYF 372 FSFS KD FVVP+W V F T D+VLFSFSDRPV EA GL RE + Sbjct: 296 FSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEALGLFREARY 342