BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0096.p (278 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 310 2e-84 SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 310 3e-84 AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene... 300 3e-81 mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 298 1e-80 AGl564 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 291 8e-79 >BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Bme Brucella melitensis Length = 301 Score = 310 bits (794), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 150/277 (54%), Positives = 196/277 (70%) Query: 1 MKLLRYGPLGQEKVGALDKSGQVRDLSLLISELTPEWLAPQRLAALAAIDLEKMPLVIDQ 60 MK LRYG GQEK G LD G +RDLS +S+L+ L P LA L A+D+ +P V Sbjct: 21 MKFLRYGAAGQEKPGLLDADGTIRDLSAHVSDLSGAALDPDALAKLGALDVNSLPKVEGN 80 Query: 61 PRFGIPFSGGRQFIAIGLNYRKHAEESGLPIPAEPVVFTKALTSMSGANDDIVMPEGSEE 120 PR G +G +FI IGLNY HA E+G +P+EP++F KA +++ G NDD+V+P GS++ Sbjct: 81 PRIGPCVAGTGKFICIGLNYSDHAAETGAAVPSEPIIFMKATSAIVGPNDDLVIPRGSQK 140 Query: 121 LDWEVELGFVIGTLARKVTREKALEHVAGYVLANDISERHWQIKRNGQWGKGKSFDSFGP 180 DWEVELG +IG A+ V+ E AL++VAGY +D+SER +QI+R GQW KGKS D+FGP Sbjct: 141 TDWEVELGIIIGKTAKYVSEEDALDYVAGYCTIHDVSERAFQIERQGQWTKGKSCDTFGP 200 Query: 181 IGPWLTTADEIPDPQILSMELSVNGVVKQSSNTADMIFPVAEIVSYLSQFMTLLPGDVVI 240 GPWL T DEI DP+ L++ L VNG Q+ ++ MI+ V +VSYLSQFM+L PGD++ Sbjct: 201 TGPWLVTKDEIADPENLAVWLKVNGETMQNGSSRTMIYGVRHLVSYLSQFMSLQPGDIIS 260 Query: 241 TGTPAGVGLGMKPAQFLKKGDVVSLRIDQLGSQTQLV 277 TGTP GVG+GMKP ++LK GDVV L I+ LGSQ Q V Sbjct: 261 TGTPPGVGMGMKPPRYLKAGDVVELGIEGLGSQKQNV 297 >SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sme Sinorhizobium meliloti Length = 281 Score = 310 bits (793), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 151/277 (54%), Positives = 194/277 (70%) Query: 1 MKLLRYGPLGQEKVGALDKSGQVRDLSLLISELTPEWLAPQRLAALAAIDLEKMPLVIDQ 60 MKLLRYG GQEK G L G +RDLS +S+L L P +L LA +D+E +P V Sbjct: 1 MKLLRYGEPGQEKPGLLGSDGIIRDLSGHVSDLAAGALDPSKLDELANLDVETLPAVSGN 60 Query: 61 PRFGIPFSGGRQFIAIGLNYRKHAEESGLPIPAEPVVFTKALTSMSGANDDIVMPEGSEE 120 PR G +G +FI IGLNY HA E+G +P EP++F KA +++ G NDD+V+P GSE+ Sbjct: 61 PRLGPCVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKATSAIVGPNDDLVLPRGSEK 120 Query: 121 LDWEVELGFVIGTLARKVTREKALEHVAGYVLANDISERHWQIKRNGQWGKGKSFDSFGP 180 DWEVELG VIG A+ V+ +AL++VAGY +D+SER +Q +R+GQW KGKS D+FGP Sbjct: 121 TDWEVELGIVIGKTAKYVSEAEALDYVAGYCTVHDVSERAFQTERHGQWTKGKSCDTFGP 180 Query: 181 IGPWLTTADEIPDPQILSMELSVNGVVKQSSNTADMIFPVAEIVSYLSQFMTLLPGDVVI 240 GPWL T DE+ DPQ L+M L VNG Q +T M++ A +VSYLSQFM+L PGD++ Sbjct: 181 TGPWLVTKDEVADPQDLAMWLKVNGETMQDGSTKTMVYGAAHLVSYLSQFMSLRPGDIIS 240 Query: 241 TGTPAGVGLGMKPAQFLKKGDVVSLRIDQLGSQTQLV 277 TGTP GVG+GMKP ++LK GDVV L I+ LGSQ Q V Sbjct: 241 TGTPPGVGMGMKPPRYLKAGDVVELGIEGLGSQKQRV 277 >AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 280 Score = 300 bits (768), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 147/278 (52%), Positives = 195/278 (70%), Gaps = 1/278 (0%) Query: 1 MKLLRYGPLGQEKVGALDKSGQVRDLSLLISELTPEWLAPQRLAALAAIDLEKMPLVIDQ 60 MKL+R G GQEK LD G+VRDLS + ++ + ++P+ L +AAIDL +P V+ + Sbjct: 1 MKLMRVGQPGQEKPAILDAEGKVRDLSAHVKDIGGDAISPEGLKKIAAIDLATLP-VLSE 59 Query: 61 PRFGIPFSGGRQFIAIGLNYRKHAEESGLPIPAEPVVFTKALTSMSGANDDIVMPEGSEE 120 R G +G +FI IGLN+ HA E+G +P EPV+F KA +++ G ND++ +P GSE+ Sbjct: 60 ERIGACVAGTGKFICIGLNFSDHAAETGATVPPEPVIFMKATSAIVGPNDNVTIPRGSEK 119 Query: 121 LDWEVELGFVIGTLARKVTREKALEHVAGYVLANDISERHWQIKRNGQWGKGKSFDSFGP 180 DWEVELG VIG A+ V+ AL++VAGY +++D+SER +Q +R GQW KGKS D+FGP Sbjct: 120 TDWEVELGVVIGKTAKYVSEADALDYVAGYCVSHDVSERAFQTERAGQWTKGKSCDTFGP 179 Query: 181 IGPWLTTADEIPDPQILSMELSVNGVVKQSSNTADMIFPVAEIVSYLSQFMTLLPGDVVI 240 IGPWL T DEI DPQ L M L VNG Q ++ M++ VA +VSYLSQFM+L PGDV+ Sbjct: 180 IGPWLVTKDEITDPQNLGMWLKVNGQTMQDGSSKTMVYGVAHVVSYLSQFMSLHPGDVIS 239 Query: 241 TGTPAGVGLGMKPAQFLKKGDVVSLRIDQLGSQTQLVV 278 TGTP GVG+G KP ++LK GDVV L I+ LGSQ Q V Sbjct: 240 TGTPPGVGMGQKPPRYLKTGDVVELGIEGLGSQKQTFV 277 >mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mlo Mesorhizobium loti Length = 281 Score = 298 bits (762), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 143/275 (52%), Positives = 190/275 (69%) Query: 1 MKLLRYGPLGQEKVGALDKSGQVRDLSLLISELTPEWLAPQRLAALAAIDLEKMPLVIDQ 60 MKLLRYG +G E+ G LD G +RDLS ++++ L P L L +D + +P V + Sbjct: 1 MKLLRYGEVGSERPGLLDADGTIRDLSAHVADIAGTTLHPASLEMLGKLDPKSLPAVSGK 60 Query: 61 PRFGIPFSGGRQFIAIGLNYRKHAEESGLPIPAEPVVFTKALTSMSGANDDIVMPEGSEE 120 PR G +G +FI IGLNY HA E+G +P EP++F KA +++ G +DD+++P GSE+ Sbjct: 61 PRLGACVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKASSAIVGPDDDVLIPRGSEK 120 Query: 121 LDWEVELGFVIGTLARKVTREKALEHVAGYVLANDISERHWQIKRNGQWGKGKSFDSFGP 180 DWEVEL VIG A+ V+ AL++VAGY +A+D+SER +Q +R GQW KGKS D+FGP Sbjct: 121 TDWEVELAVVIGKTAKYVSEADALDYVAGYAVAHDVSERAFQAERQGQWTKGKSCDTFGP 180 Query: 181 IGPWLTTADEIPDPQILSMELSVNGVVKQSSNTADMIFPVAEIVSYLSQFMTLLPGDVVI 240 GPWL T DE+ DPQ L M L+VNG Q+ +T M++ V +VSYLSQFM+L PGD++ Sbjct: 181 TGPWLVTKDEVADPQNLKMWLTVNGKTMQNGSTKTMVYGVKYLVSYLSQFMSLHPGDIIS 240 Query: 241 TGTPAGVGLGMKPAQFLKKGDVVSLRIDQLGSQTQ 275 TGTP GVGLGMKP FL+ GDVV L I+ LG Q Q Sbjct: 241 TGTPPGVGLGMKPPVFLRAGDVVELGIEGLGQQKQ 275 >AGl564 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 282 Score = 291 bits (746), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 150/277 (54%), Positives = 186/277 (67%) Query: 1 MKLLRYGPLGQEKVGALDKSGQVRDLSLLISELTPEWLAPQRLAALAAIDLEKMPLVIDQ 60 MKLLRYGPLGQEK ALD G +RDLS +IS++ L+ L LA IDL +P V Sbjct: 1 MKLLRYGPLGQEKPAALDAEGNIRDLSSVISDIAGYSLSRSSLDKLAKIDLSSLPSVPSD 60 Query: 61 PRFGIPFSGGRQFIAIGLNYRKHAEESGLPIPAEPVVFTKALTSMSGANDDIVMPEGSEE 120 R G F+A+GLNY HA E+ P+P EPV+F K + +SG ND I + + SE+ Sbjct: 61 VRIGPCVGAVGNFVAVGLNYIDHALETNTPVPDEPVLFNKHTSCISGPNDPICLLKDSEK 120 Query: 121 LDWEVELGFVIGTLARKVTREKALEHVAGYVLANDISERHWQIKRNGQWGKGKSFDSFGP 180 DWEVE+ FVIG A V E AL VAG+ + +D+SER +QI+R GQW KGKS +FGP Sbjct: 121 TDWEVEIAFVIGEPAYHVREEDALSVVAGFCVCHDVSERAFQIERGGQWTKGKSGPTFGP 180 Query: 181 IGPWLTTADEIPDPQILSMELSVNGVVKQSSNTADMIFPVAEIVSYLSQFMTLLPGDVVI 240 +GPWL T DEI D Q L + L VNG Q+ +T+ MIFP+A +VSY+S+FM L+PGDV+ Sbjct: 181 LGPWLVTPDEISDVQNLDLWLDVNGKRMQTGSTSRMIFPIATLVSYISRFMRLMPGDVIT 240 Query: 241 TGTPAGVGLGMKPAQFLKKGDVVSLRIDQLGSQTQLV 277 TGTP GVGLGM P FLK GDVV L ID LG Q Q+V Sbjct: 241 TGTPPGVGLGMNPPVFLKAGDVVELGIDGLGRQRQIV 277