BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0075.p (301 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanac... 227 2e-59 TP0229 Non COG 147 3e-35 CAC2997 [L] COG1573 Uracil-DNA glycosylase Cac Clostridium aceto... 143 4e-34 RC1309 [L] COG1573 Uracil-DNA glycosylase Rco Rickettsia conorii 142 7e-34 RP845 [L] COG1573 Uracil-DNA glycosylase Rpr Rickettsia prowazekii 135 1e-31 >RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanacearum Length = 292 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 117/208 (56%), Positives = 139/208 (66%), Gaps = 8/208 (3%) Query: 91 MPNDDEDDEPVIRINPAIATMDWAKLKETVAECRQCGLCNGRKKTVFGVGDEKAKWLFIG 150 +P D D PV IA DWA+L+ V+ C C LC R +TVFGVGD +A W+ IG Sbjct: 89 VPTDSTD--PVAPRAQRIAAFDWAQLEAAVSGCTACKLCERRTQTVFGVGDRQADWMLIG 146 Query: 151 EGPGRNEDMQGEPFVGPAGKLLDNMLVAMGLKRGENAYIANIVKCRPTDEAGKDRPPSAQ 210 E PG ED QGEPFVG AGKLLD+ML A+GL R +IAN++KCRP +R P Sbjct: 147 EAPGEQEDRQGEPFVGQAGKLLDSMLRAIGLSRETGVFIANVLKCRPPG----NRDPEPD 202 Query: 211 EVASCLPYLQRQIALIQPTVLVALGKTAALSLLGLDPATPVSKLRGTVHRYQGLPLVVTY 270 EVA C PYL+RQIALI+P V+V LG+ AA SL L TPV KLRG VH G+P+VVTY Sbjct: 203 EVAMCDPYLKRQIALIKPRVIVVLGRFAAQSL--LQTQTPVGKLRGKVHEVDGVPVVVTY 260 Query: 271 HPAYLLRQLGDKSKAWADLCLAMTTYAD 298 HPAYLLR L DK++AW DLCLA Y + Sbjct: 261 HPAYLLRTLTDKARAWEDLCLARKVYTE 288 >TP0229 Non COG Length = 277 Score = 147 bits (371), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 77/174 (44%), Positives = 98/174 (56%), Gaps = 6/174 (3%) Query: 116 LKETVAECRQCGLCNGRKKTVFGVGDEKAKWLFIGEGPGRNEDMQGEPFVGPAGKLLDNM 175 L+E + CR C L R V G G A L +GE PG ED G PFVG +GKLLD M Sbjct: 92 LREEIFACRACELYQRRTHAVVGEGVADADVLVVGEAPGAEEDRSGRPFVGRSGKLLDAM 151 Query: 176 LVAMGLKRGENAYIANIVKCRPTDEAGKDRPPSAQEVASCLPYLQRQIALIQPTVLVALG 235 L A+GL R +N YI N+VKCRP ++R P+ E A C +L + L +P ++ LG Sbjct: 152 LAAIGLSRQQNCYITNVVKCRPP----RNRTPTPHETACCARFLHAHLTLHRPCAILVLG 207 Query: 236 KTAALSLLGLDPATPVSKLRGTVHRYQGLPLVVTYHPAYLLRQLGDKSKAWADL 289 + AA + L + KLRG YQG+PL+ TYHP+ LLR K AW DL Sbjct: 208 RCAAQHM--LQTTDGIGKLRGRFFTYQGIPLLATYHPSALLRDEALKRPAWEDL 259 >CAC2997 [L] COG1573 Uracil-DNA glycosylase Cac Clostridium acetobutylicum Length = 206 Score = 143 bits (361), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 79/208 (37%), Positives = 111/208 (53%), Gaps = 24/208 (11%) Query: 111 MDWAKLKETVAECRQCGLCNGRKKTVFGVGDEKAKWLFIGEGPGRNEDMQGEPFVGPAGK 170 + W +L EC++C L R VFG G+ +AK +F+GE PG +ED G PFVG AG+ Sbjct: 2 LQWRELYNECIECQKCRLGENRTNMVFGEGNPEAKLMFVGEAPGADEDRLGRPFVGRAGQ 61 Query: 171 LLDNMLVAMGLKRGENAYIANIVKCRPTDEAGKDRPPSAQEVASCLPYLQRQIALIQPTV 230 LL L A+ L R + YIAN+ KCRP K+R P E +C+PYL+ Q ALI+P + Sbjct: 62 LLTKGLTALNLSRDRDYYIANVCKCRPE----KNRTPYEDEAEACMPYLRNQFALIKPKI 117 Query: 231 LVALGKTA-------AL-------------SLLGLDPATPVSKLRGTVHRYQGLPLVVTY 270 LV LG TA AL +L+ + +++ RG + +G+ ++ T+ Sbjct: 118 LVCLGSTAMKFIMEPALNKILQQNNTIEEENLIWKNGQMRITRDRGKWLKVKGIYMMATF 177 Query: 271 HPAYLLRQLGDKSKAWADLCLAMTTYAD 298 HPA L R K+ W DL L Y D Sbjct: 178 HPAALFRDENKKTAFWEDLKLIKAKYVD 205 >RC1309 [L] COG1573 Uracil-DNA glycosylase Rco Rickettsia conorii Length = 279 Score = 142 bits (359), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 86/221 (38%), Positives = 122/221 (55%), Gaps = 15/221 (6%) Query: 75 PWLNEMSDADMAPPGFMPNDDEDDEPVIRINPAIATMDWAKLKETVAECRQCGLCNGRKK 134 P + MS + A F ND D + + A + +LKE++ C L Sbjct: 54 PQVKLMSTKETA---FNMNDKVHDNITLARSLADKANNIEELKESLLNFNGCELKKFATN 110 Query: 135 TVFGVGDEKAKWLFIGEGPGRNEDMQGEPFVGPAGKLLDNMLVAMGLKRGENAYIANIVK 194 TVFG G+ +AK + IGE PG ED++G PF G +G LLDNML A+G+ R NAYI N V Sbjct: 111 TVFGDGNLEAKIMLIGEAPGSTEDLKGIPFYGESGNLLDNMLHAIGISRKNNAYITNTVF 170 Query: 195 CRPTDEAGKDRPPSAQEVASCLPYLQRQIALIQPTVLVALGKTAALSLLGLDPATPVSKL 254 RP +R P+ +EV C P++++ IALI P +++ +G TAA SLLG + ++K+ Sbjct: 171 WRPP----ANRQPTFEEVDICRPFVEKHIALINPQLIILVGSTAATSLLGKNSG--ITKI 224 Query: 255 RGTVH----RYQGLPLVVT--YHPAYLLRQLGDKSKAWADL 289 R + +Y P+ T +HPAYLLRQ K +W DL Sbjct: 225 RQEYYFYTNKYLSAPIQTTAIFHPAYLLRQPMQKRTSWYDL 265 >RP845 [L] COG1573 Uracil-DNA glycosylase Rpr Rickettsia prowazekii Length = 258 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 78/203 (38%), Positives = 115/203 (56%), Gaps = 13/203 (6%) Query: 93 NDDEDDEPVIRINPAIATMDWAKLKETVAECRQCGLCNGRKKTVFGVGDEKAKWLFIGEG 152 N++ D + + A + +L+E++ C L TVFG G+ +A + IGE Sbjct: 49 NNNVHDNITLSRSLADKANNIVELRESLLNFNGCELKKFATNTVFGDGNPQANIMLIGEA 108 Query: 153 PGRNEDMQGEPFVGPAGKLLDNMLVAMGLKRGENAYIANIVKCRPTDEAGKDRPPSAQEV 212 PG ED++G PF G +G LLDNML A+G+ R N YI N+V RP +R P+ +EV Sbjct: 109 PGNTEDLKGIPFCGESGNLLDNMLYAIGISRN-NFYITNMVFWRPP----ANRQPTLEEV 163 Query: 213 ASCLPYLQRQIALIQPTVLVALGKTAALSLLGLDPATPVSKLRGTVH----RYQGLPLVV 268 C P++++ IALI P +L+ +G TAA SLLG + ++K+R + +Y P+ Sbjct: 164 DICRPFVEKHIALINPKLLILVGSTAATSLLGKNAC--ITKIRQEYYFYTNKYISTPIQT 221 Query: 269 T--YHPAYLLRQLGDKSKAWADL 289 T +HPAYLLRQ K +W DL Sbjct: 222 TAIFHPAYLLRQPMQKRTSWYDL 244