BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0037.p (634 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value slr2002_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP... 302 2e-81 all3879_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP... 278 3e-74 alr0573 [M] COG1181 D-alanine-D-alanine ligase and related ATP-g... 129 3e-29 CAC1540 [M] COG1181 D-alanine-D-alanine ligase and related ATP-g... 112 3e-24 BH3654_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP-... 99 3e-20 >slr2002_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Syn Synechocystis Length = 463 Score = 302 bits (773), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 170/391 (43%), Positives = 235/391 (60%), Gaps = 10/391 (2%) Query: 4 IEFLRILPLHGPNIWTYR--PALEVWIDIGELEDFPSNIIPGFNERLTGWLPSLVEHRCS 61 ++ L+ L L GPN W+ R + + +D+ +L + PSN IPGF E L LPSLVEH CS Sbjct: 1 MKILKTLTLRGPNYWSIRRKKLIVMRLDLEDLAERPSNSIPGFYEGLIRVLPSLVEHFCS 60 Query: 62 YGERGGFLQRLKEGTWPGHIMEHVTLELQNLAGLPGGFGKARETSTRGVYKVVVRARHEQ 121 G RGGFL R++EGT+ GHI+EHV LELQ L G+ GFG+ RETST G+Y VV EQ Sbjct: 61 PGHRGGFLARVREGTYMGHIVEHVALELQELVGMTAGFGRTRETSTPGIYNVVYEYVDEQ 120 Query: 122 VTRAALYAGRDLVMAAIEDKPF---DVDATIEKLRDMVDSLCLGPSTACIVDAADERRIP 178 R A A L + ++ + +++ +E LRD+ + LGPST IV AD R+IP Sbjct: 121 AGRYAGRAAVRLCRSLVDTGDYSLTELEKDLEDLRDLGANSALGPSTETIVTEADARKIP 180 Query: 179 SIRLSEGNLVQLGHGCKQRRIWTAETDQTSAIAESISRDKDLTKTLLQSCGVAIPEGRIV 238 + LS +VQLG+G Q+RI + + + ++ DK+ TKT+LQ G+ +P G + Sbjct: 181 WMLLSARAMVQLGYGVHQQRIQATLSSHSGILGVELACDKEGTKTILQDAGIPVPRGTTI 240 Query: 239 DSPADAWEAAEDI-GLPVVVKPSDANHGRGVFIDLNTQAEVETAYKAALEEGS--SVLVE 295 D EA D+ G PVV+KP D NHGRG+ I++ E AY A EE S++VE Sbjct: 241 QYFDDLEEAINDVGGYPVVIKPLDGNHGRGITINVRHWEEAIAAYDLAAEESKSRSIIVE 300 Query: 296 RFIRGNEHRLLIVGGKLAAAARGEPIFVVGDGVSTISQLIDVVNMDPRRGDLEEHPLNPV 355 R+ G++HR+L+V GKL A A P V GDG STI++LID N DP RGD + L + Sbjct: 301 RYYEGSDHRVLVVNGKLVAVAERIPAHVTGDGTSTITELIDKTNQDPNRGDGHANILTKI 360 Query: 356 ILDREPAARLELERQGLSADSVPPNGKKVLL 386 ++++ A +ERQG + DSV P + V L Sbjct: 361 VVNK--TAIDVMERQGYNLDSVLPKDEVVYL 389 >all3879_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Nos Nostoc sp. PCC 7120 Length = 479 Score = 278 bits (711), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 161/384 (41%), Positives = 236/384 (61%), Gaps = 8/384 (2%) Query: 4 IEFLRILPLHGPNIWTYR--PALEVWIDIGELEDFPSNIIPGFNERLTGWLPSLVEHRCS 61 + L+I L GPN W+ R + + +D+ L + PSN IPGF E L LPSL H CS Sbjct: 1 MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCS 60 Query: 62 YGERGGFLQRLKEGTWPGHIMEHVTLELQNLAGLPGGFGKARETSTRGVYKVVVRARHEQ 121 G GGFL R++EGT GHI+EHV LELQ LAG+ GFG+ RET+T G+Y+VV+ +E+ Sbjct: 61 PGCHGGFLMRVREGTMMGHIVEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEE 120 Query: 122 VTRAALYAGRDLVMAAIEDKPF---DVDATIEKLRDMVDSLCLGPSTACIVDAADERRIP 178 R A A L + ++ + +++ I+ L+D+ LGPST IV A++R IP Sbjct: 121 AGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRDASLGPSTEAIVKEAEKRGIP 180 Query: 179 SIRLSEGNLVQLGHGCKQRRIWTAETDQTSAIAESISRDKDLTKTLLQSCGVAIPEGRIV 238 ++LS L+QLG+G +R+ TD+T + ++ DK+ TK +L + GV +P G ++ Sbjct: 181 WMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI 240 Query: 239 DSPADAWEAAEDIG-LPVVVKPSDANHGRGVFIDLNTQAEVETAYKAALEEGSSVLVERF 297 + D EA E +G P+V+KP D NHGRG+ ID+ + E E AY+AA + S++VER+ Sbjct: 241 NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERY 300 Query: 298 IRGNEHRLLIVGGKLAAAARGEPIFVVGDGVSTISQLIDVVNMDPRRGDLEEHPLNPVIL 357 G +HR+L+V GK+ A A P V+G+G ST+++LI+ +N DP RGD + L + L Sbjct: 301 YVGRDHRVLVVDGKVVAVAERVPAHVIGNGRSTVAELIEEINQDPNRGDGHDKVLTKIEL 360 Query: 358 DREPAARLELERQGLSADSVPPNG 381 DR + LER G + +SVPP G Sbjct: 361 DR--TSYQLLERAGYTLNSVPPKG 382 >alr0573 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Nos Nostoc sp. PCC 7120 Length = 636 Score = 129 bits (323), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 99/281 (35%), Positives = 142/281 (50%), Gaps = 8/281 (2%) Query: 112 KVVVRARHEQVTRAALYAGRDLVMAAIEDKPFDVDATIEKLRDMV-DSLCLGPSTACIVD 170 K+ V++ HE+ R+ +Y D A +D F D + L++ S+ GP+ ++ Sbjct: 110 KIAVQSLHERTIRSVVYLVWDWFEAINQDDDFFWDDQLVTLQNRFRQSVYGGPTVYALLR 169 Query: 171 AADERRIPSIRLSEGNLVQLGHGCKQRRIWTAETDQTSAIAESISRDKDLTKTLLQSCGV 230 A E+ IP+ L + L+Q G G K R + S I + KD K L + G Sbjct: 170 TAYEKGIPTFYLWDEGLMQYGWGKKHIRGVATTFNCDSHIDSDFTTRKDDCKAFLHTLGF 229 Query: 231 AIPEGRIVDSPADAWEAAEDIGLPVVVKPSDANHGRGVFIDLNTQAEVETAYKAALE--- 287 +P+G IV SP +A + A+DIG PV VKP + G GV D+ E+E AY ALE Sbjct: 230 PVPQGEIVYSPKEARQVAKDIGYPVAVKPVVGHKGIGVTADVKDVDELEVAYDRALEAIP 289 Query: 288 --EGSSVLVERFIRGNEHRLLIVGGKLAAAARGEPIFVVGDGVSTISQLIDVVNMDPRRG 345 E + ++VE+ I+G + RLL V G+ AA P VVGDG STI +LI N R Sbjct: 290 ENEPARIIVEKSIKGKDFRLLCVNGRFVAATERHPASVVGDGDSTIWELIQKENRKAVRL 349 Query: 346 DLEEHPLNPVILDREPAARLELERQGLSADSVPPNGKKVLL 386 D P++ +I+D A LE Q LS +V G+ V L Sbjct: 350 DTPTSPMSKIIVD--DAMEHYLEEQRLSLKTVLDKGETVHL 388 >CAC1540 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Cac Clostridium acetobutylicum Length = 328 Score = 112 bits (279), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 80/238 (33%), Positives = 117/238 (49%), Gaps = 7/238 (2%) Query: 157 DSLCLGPSTACIVDAADERRIP-SIRLSEGNLVQLGHGCKQRRIWTA-ETDQTSAIAESI 214 D L ST +++ A +R+I I E N ++L G K + A +T + IA I Sbjct: 4 DYTKLELSTQILINEALDRKIQVDILDMEDNFIRLKKGDKVEYVKQATKTSLDNYIAPLI 63 Query: 215 SRDKDLTKTLLQSCGVAIPEGRIVDSPADAWEAAEDI-GLPVVVKPSDANHGRGVFI--D 271 +K++TK +L+ G+ +PEG + ++A E G VVKP N G+GV I Sbjct: 64 MENKEVTKIVLKENGINVPEGVTLKEASEAKEKFSLFYGKDTVVKPKSTNFGKGVIILKQ 123 Query: 272 LNTQAEVETAYKAALEEGSSVLVERFIRGNEHRLLIVGGKLAAAARGEPIFVVGDGVSTI 331 L + + E A A E +SV+VE FI G E+R L++G + A P V+GDGV +I Sbjct: 124 LRSIEDFENAVNQAFEYDNSVMVEEFIAGKEYRFLVIGDETIAVLHRVPANVIGDGVHSI 183 Query: 332 SQLIDVVNMDPRRGDLEEHPLNPVILDREPAARLELERQGLSADSVPPNGKKVLLLRN 389 +L+D N DPRRG PL ++L L + D VP + V L N Sbjct: 184 KELVDEKNKDPRRGQGYVKPLEKIVLGTIEKEYLSFSNRDF--DYVPDKNEIVYLREN 239 >BH3654_1 [M] COG1181 D-alanine-D-alanine ligase and related ATP-grasp enzymes Bha Bacillus halodurans Length = 380 Score = 99.0 bits (245), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 64/203 (31%), Positives = 109/203 (53%), Gaps = 11/203 (5%) Query: 190 LGHGCKQRRIWTAETDQTSAIAESISRDKDLTKTLLQSCGVAIPEGRIVD---SPADAWE 246 L + K+ + + D+ + A SI +KD+TK L+ V +PEG+ + + +D + Sbjct: 57 LANEEKEHQFAVSRGDKVTKEAISICINKDITKEYLEKANVPVPEGKGFNEAQADSDIIQ 116 Query: 247 AAEDIGLPVVVKPSDANHGRGVFIDLNTQAEVETAYKAALEE--GSSVLVERFIRGNEHR 304 A+++G PVV+KP+D G+GV +++ + + A E+ S+V+VER+ G + R Sbjct: 117 YAKELGFPVVIKPTDGLSGKGVVVNIQNEEMLREAISYVREQLAYSNVVVERYFEGEDFR 176 Query: 305 LLIVGGKLAAAARGEPIFVVGDGVSTISQLIDVVNMDPRRG-DLEEHPLNPVILDREPAA 363 + I+G ++ A P VVGDG TI QLI + N + +L+ P+ LD E Sbjct: 177 IYIMGDQVIGAVTRVPANVVGDGKRTIKQLIKLKNKARNKNPNLQRRPIK---LDHELEK 233 Query: 364 RLELERQGLSADSVPPNGKKVLL 386 L L + DS+P G++V+L Sbjct: 234 TLNL--INYTIDSIPSKGERVIL 254