BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0254.p (448 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0536 [GEPR] COG0477 Permeases of the major facilitator superf... 355 1e-97 PA4343 [GEPR] COG0477 Permeases of the major facilitator superfa... 353 3e-97 RSc3059 [GEPR] COG0477 Permeases of the major facilitator superf... 283 5e-76 RSc3060 [GEPR] COG0477 Permeases of the major facilitator superf... 267 3e-71 Z5523 [GEPR] COG0477 Permeases of the major facilitator superfam... 260 4e-69 >RSp0536 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 441 Score = 355 bits (910), Expect = 1e-97, Method: Compositional matrix adjust. Identities = 192/410 (46%), Positives = 271/410 (66%), Gaps = 4/410 (0%) Query: 29 KEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGISFVIRPLGAI 88 + A +GNA E++D +YG+FA VI + FP+ + T+LLL++ TFG+ FV RPLG I Sbjct: 20 HAVATAILGNAFEWFDFTLYGFFAPVIATVLFPSANPFTALLLAIATFGVGFVTRPLGGI 79 Query: 89 VLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFSTGGE 148 VLG YADR GR+ +L +S L+M +GTA+IA APTYAQ GIA+P+I ++ARLLQGFS GGE Sbjct: 80 VLGIYADRRGRRPALALSALLMALGTALIAIAPTYAQAGIAAPVIAVIARLLQGFSAGGE 139 Query: 149 FGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTDVQLNDWGWRVAF 208 G ATA++ E A +R F+ SW ++ GLA V A + L + L L WGWR+ F Sbjct: 140 MGGATAYLNEIAPPGKRAFYTSWIQASVGLAIVCGAMLGTLATSLLDAEALRAWGWRIPF 199 Query: 209 CFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDRVKLLLAIGVVAG 268 G+L+GP+G IRS++DETP F + A E A+A+ SPL+ V+ R R +L+ +V Sbjct: 200 ALGVLIGPIGYVIRSRLDETPAFARAATE---ARAD-SPLLEVVRRHRRAVLIGASIVIL 255 Query: 269 ATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTI 328 T YV YM TYA+ L +P+++ F G++ +G+ +MLTAP+FG L+DR G RVL+ Sbjct: 256 WTVCTYVLLFYMQTYAIHVLKLPSSAGFAGSMASGVTMMLTAPLFGWLADRLGYQRVLSG 315 Query: 329 AMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTETRGTGL 388 A ++I + +YPL+ LN P TLI VQ + G+L AA GPIP +D+FPT+ TGL Sbjct: 316 AALAILVLAYPLYAYLNHAPAAGTLIGVQIVFGLLSAAYTGPIPVAFADLFPTKVLSTGL 375 Query: 389 ALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVIVL 438 + +YN +V L GGF+P +TW+ A + +AP YVM AL+SL+ + L Sbjct: 376 STAYNLTVMLIGGFAPFYLTWLSHAFDPMLAPVLYVMVAALVSLIGTLWL 425 >PA4343 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 439 Score = 353 bits (907), Expect = 3e-97, Method: Compositional matrix adjust. Identities = 189/420 (45%), Positives = 271/420 (64%), Gaps = 6/420 (1%) Query: 29 KEICAASIGNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGISFVIRPLGAI 88 +++ AA IGNALE+YD ++YG+ + +I +LFFP+ DE TSLL+++ TFG+ F +RP+G + Sbjct: 12 RQVAAAVIGNALEWYDFIVYGFLSSLIARLFFPSGDEYTSLLMALATFGVGFFMRPVGGV 71 Query: 89 VLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFSTGGE 148 +LG YADR GRKA++ + IL+M + AMIAFAPTYA IG+ +PL+I++AR+LQGF+TGGE Sbjct: 72 LLGLYADRRGRKAAMQLIILLMTLSIAMIAFAPTYAAIGVGAPLLIVIARMLQGFATGGE 131 Query: 149 FGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTDVQLNDWGWRVAF 208 + +ATAF+VE A RRG + SWQL Q LA AG+ AL+++ L L WGWRV F Sbjct: 132 YASATAFLVESAPPHRRGLYGSWQLFGQCLAVFAGAGMGALVTHCLDAEALESWGWRVPF 191 Query: 209 CFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDRVKLLLAIGVVAG 268 FGLL+GPVGL+IR + ET F EA E+ L+ VL R +L+++G+ Sbjct: 192 LFGLLIGPVGLWIRRYMGETEAFL----EAIREPGERQGLLGVLREYRRSVLVSMGLTVV 247 Query: 269 ATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTI 328 T YV + MPT+A KQL +P F+ + ++ L P+ G LSDR GR VL + Sbjct: 248 GTVSFYVVLVNMPTFAHKQLGLPLDEVFMVQMAAVALMTLVIPLAGGLSDRVGRRPVLLV 307 Query: 329 AMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTETRGTGL 388 A ++ L YPLF + P++ L+ +Q ++ I GP P +++ FP R TGL Sbjct: 308 ATLAFMLMVYPLFAWVAAAPSLGRLLLMQLLLCTAIGGFFGPAPTAVAEQFPVRVRSTGL 367 Query: 389 ALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVIVLGRRMGRHPTP 448 A++YN +V LFGGF+P IVTW+ + +AP+FYV+ A + L+A + L R G P P Sbjct: 368 AVAYNLAVMLFGGFAPFIVTWLTEVGGSPVAPAFYVLGAAFLGLLATLYL--REGATPAP 425 >RSc3059 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 431 Score = 283 bits (725), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 166/429 (38%), Positives = 247/429 (57%), Gaps = 17/429 (3%) Query: 25 KNPWKEICAASI-----GNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGIS 79 NP E A SI GN LE YD ++YGY+A I FFP +E SL+LS+ TFG+ Sbjct: 4 HNP-NESKARSIFRVVSGNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVG 62 Query: 80 FVIRPLGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARL 139 F++RPLGA++LG+Y DR GR+A L +++ +M GT +IA P YA IG+A+PL++++ RL Sbjct: 63 FLMRPLGALILGAYIDRHGRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRL 122 Query: 140 LQGFSTGGEFGAATAFMVEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTDVQL 199 LQGFS G E G + ++ E + R+GFF SWQ ++Q +A + AA + LLS+ L+ ++ Sbjct: 123 LQGFSAGVELGGVSVYLAEISTPGRKGFFVSWQSASQQVAVMFAALLGVLLSFQLSPKEM 182 Query: 200 NDWGWRVAFCFGLLVGPVGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDRVKL 259 +WGWRV F G L+ P IR + ET EFK+ A+ + +S + + +L Sbjct: 183 GEWGWRVPFLVGCLIVPFLFLIRRSLQETEEFKQRKHRPALGEIFRS------MGENWRL 236 Query: 260 LLA--IGVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLS 317 + A + VV +F Y+ Y PT+ LH+ S I L G PV G++S Sbjct: 237 VGAGTLLVVMTTVSF-YLITAYTPTFGKSVLHLSNLDSLIVTLCVGASNFFWLPVMGSVS 295 Query: 318 DRYGRFRVLTIAMVSIG-LTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLS 376 DR GR R L I ++ LT+YP L P+ L+AV+ + L + G + L+ Sbjct: 296 DRIGR-RPLLITFTALTLLTAYPAMQWLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLT 354 Query: 377 DIFPTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVI 436 +I P R TG +L+Y+ + +FGGF+P I TW+I AT NK P ++ A+ LVA + Sbjct: 355 EIMPPAVRTTGFSLAYSLATAIFGGFTPAIATWLIHATGNKAMPGLWLSFAAICGLVATL 414 Query: 437 VLGRRMGRH 445 ++ + GRH Sbjct: 415 IVVKPAGRH 423 >RSc3060 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 433 Score = 267 bits (683), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 152/401 (37%), Positives = 230/401 (57%), Gaps = 7/401 (1%) Query: 37 GNALEFYDLLIYGYFAVVIGKLFFPTQDETTSLLLSVGTFGISFVIRPLGAIVLGSYADR 96 GN LE +D ++YGY+A I FFP + SL+LS+ TFG F++RPLGAIVLG+Y DR Sbjct: 20 GNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAIVLGAYIDR 79 Query: 97 AGRKASLTVSILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFSTGGEFGAATAFM 156 GR+A L +++++M GT +IA P Y IG+A+PL++++ RLLQGFS GGE G + ++ Sbjct: 80 HGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGEPGGVSVYL 139 Query: 157 VEHADARRRGFFASWQLSTQGLATVLAAGVSALLSYNLTDVQLNDWGWRVAFCFGLLVGP 216 E A R+GF+ SWQ +Q +A + AA + LLS+ L+ ++ +WGWRV F G L+ P Sbjct: 140 SEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPKEMGEWGWRVPFLIGCLIVP 199 Query: 217 VGLYIRSQIDETPEFKKIAAEAAVAKAEKSPLMLVLVRDRVKLLLAIGVVAGATAFN-YV 275 +IR + ET EF++ + + +S + + +L A ++A T + Y+ Sbjct: 200 FLFFIRRSLRETEEFEQRKHRPTMGELLRS------MGENWRLAGAGTLLAVMTTVSFYL 253 Query: 276 HKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTIAMVSIGL 335 Y PT+ LH+ S I L G ++ PV G+LSDR GR L + L Sbjct: 254 ITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLIVFSALTLL 313 Query: 336 TSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTETRGTGLALSYNFS 395 T+YP L P+ L+AV+ + L G + L++I P + R TG +L+Y+ + Sbjct: 314 TAYPAMTWLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSLAYSLA 373 Query: 396 VTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVI 436 +FGGF+P I T +I AT NK P ++ A+ LVA + Sbjct: 374 TAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATL 414 >Z5523 [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 260 bits (665), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 163/435 (37%), Positives = 254/435 (58%), Gaps = 20/435 (4%) Query: 14 TAATATTAAKRKNP--WKEICAASIGNALEFYDLLIYGYFAVVIGKLFFP-TQDETTSLL 70 TA T + +K P + I A++ GNALEF+D +Y +F V I LFFP + D+ +LL Sbjct: 2 TAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALL 61 Query: 71 LSVGTFGISFVIRPLGAIVLGSYADRAGRKASLTVSILIMMVGTAMIAFAPTYAQIGIAS 130 L+ TFG+SF +RPLG I++G++ADR GRK ++ +I +M +GT MI APTY G Sbjct: 62 LAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWG 121 Query: 131 PLIIIVARLLQGFSTGGEFGAATAFMVEHADARRRGFFASWQLSTQGLAT----VLAAGV 186 +++ARL+QG + GGE GA+ + +VE A A RRGF++SW L+TQGLAT V+A G+ Sbjct: 122 TATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALGL 181 Query: 187 SALLSYNL-TDVQLNDWGWRVAFCFGLLVGPVGLYIR-SQIDETPEFKKIAAEAAVAKAE 244 SA L + ++ + +WGWRV F G+L+ PVG ++R S ++ PE A A A Sbjct: 182 SAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPE----PAHNKKAAAS 237 Query: 245 KSPLMLVLVRDRVKLLLAIGVVAGATAFNYVHKLYMPTYALKQLHIPATSSFIGALITGL 304 +S L L++ + + I + G+T Y+ Y T+A K L + S L+ G+ Sbjct: 238 ESAFSL-LMQHKATIANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGV 296 Query: 305 VLMLTAPVFGTLSDRYGRFRVLTIAMVSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLI 364 + + A + G L D GR +++ I+ V + + S+P F LL +P+ L+ V V V++ Sbjct: 297 ITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFWLLVNYPSPGMLLTV---VFVMV 353 Query: 365 A-ACLGPIPAML--SDIFPTETRGTGLALSYNFSVTLFGGFSPLIVTWMIAATENKMAPS 421 + LG +P ML S++ P R G AL Y+ V +FGGF+ T I ++ AP+ Sbjct: 354 SFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPA 413 Query: 422 FYVMATALISLVAVI 436 +Y+ L+S++A++ Sbjct: 414 WYLGGGTLLSMLALL 428