BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0222.p (234 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI1235 [IQR] COG1028 Dehydrogenases with different specificiti... 315 6e-86 AGc2465 [IQR] COG1028 Dehydrogenases with different specificitie... 305 6e-83 SMc00136 [IQR] COG1028 Dehydrogenases with different specificiti... 287 2e-77 mll1830 [IQR] COG1028 Dehydrogenases with different specificitie... 272 5e-73 DR1943 [IQR] COG1028 Dehydrogenases with different specificities... 122 5e-28 >BMEI1235 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bme Brucella melitensis Length = 241 Score = 315 bits (806), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 148/234 (63%), Positives = 183/234 (78%) Query: 1 MSTTKVALVIGGGSGMGAGVAQKLAADGFGIGILSSSGKGESLGNSLGGVGVTGSNRSVA 60 M+ KVA++ GGSGMGA A++LA DGF + ILSSSGKGE+L LGG+GVTGSN+S Sbjct: 8 MTIQKVAIITAGGSGMGAASARRLAQDGFAVAILSSSGKGEALAKELGGIGVTGSNQSND 67 Query: 61 DLKKAVDACLARWGRIDVLVNSAGHGPKGKVLELSDEEWLLGMEVYLMNVVRSARLVTPL 120 DL+K VD L +WGRIDVLVNSAGHGP+ +LE++DE+W GM+ Y +N VR ARLV P Sbjct: 68 DLQKLVDQTLEKWGRIDVLVNSAGHGPRAPILEITDEDWHKGMDTYFLNAVRPARLVVPA 127 Query: 121 MQQQGGGAIINISSFSAVEPSQSFPTSSVFRAGLSAYTKLFADKYAPENIRMNNILPGFI 180 MQ+Q G IINIS+ A EPS FPTS+VFRAGL+++TK+FAD YA ENIRMNN+LPG+I Sbjct: 128 MQKQKSGVIINISTAWAFEPSAMFPTSAVFRAGLASFTKIFADTYAAENIRMNNVLPGWI 187 Query: 181 NSLPEKPGIKELIPMARYGKVSEVAALASHLASDAAAYITGQNIRIDGGITRSL 234 +SLP +E +PM RYGK E+AA S LASD AAYITGQN+R+DGG+TRS+ Sbjct: 188 DSLPTTEERRESVPMQRYGKSEEIAATVSFLASDGAAYITGQNLRVDGGLTRSV 241 >AGc2465 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 248 Score = 305 bits (781), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 141/234 (60%), Positives = 186/234 (79%) Query: 1 MSTTKVALVIGGGSGMGAGVAQKLAADGFGIGILSSSGKGESLGNSLGGVGVTGSNRSVA 60 MS KVA+V GGSGMGA VA++LAADG+ + ILSSSGKGE+L LGG+GVTGSN+S Sbjct: 15 MSAEKVAIVTAGGSGMGAAVAKRLAADGYRLAILSSSGKGEALAKELGGIGVTGSNQSND 74 Query: 61 DLKKAVDACLARWGRIDVLVNSAGHGPKGKVLELSDEEWLLGMEVYLMNVVRSARLVTPL 120 DL++ D L ++GRIDVLVNSAGHGP+ +L+++DE+W G++VYLMNV+R R+V P+ Sbjct: 75 DLQRLTDQTLEKFGRIDVLVNSAGHGPRASILDITDEQWHTGLDVYLMNVIRPTRIVAPI 134 Query: 121 MQQQGGGAIINISSFSAVEPSQSFPTSSVFRAGLSAYTKLFADKYAPENIRMNNILPGFI 180 M +Q GAI+NIS+ A EPS FPTS+VFRAGL++Y+K+FAD YA +N+RMNN+LPG+I Sbjct: 135 MVKQKAGAIVNISTAWAFEPSSMFPTSAVFRAGLASYSKIFADTYAADNVRMNNVLPGWI 194 Query: 181 NSLPEKPGIKELIPMARYGKVSEVAALASHLASDAAAYITGQNIRIDGGITRSL 234 +SLP ++ +PM RYGK E+AA + LAS+ A YITGQNIR+DGG+TRS+ Sbjct: 195 DSLPATEERRDSVPMQRYGKSEEIAATVAFLASEGAGYITGQNIRVDGGLTRSV 248 >SMc00136 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 234 Score = 287 bits (734), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 149/234 (63%), Positives = 183/234 (78%) Query: 1 MSTTKVALVIGGGSGMGAGVAQKLAADGFGIGILSSSGKGESLGNSLGGVGVTGSNRSVA 60 MS KVA+V GGSGMGAG A+++AA+G+ + ILSSSGKGE+L LGG+GVTGSN+S Sbjct: 1 MSEQKVAIVTAGGSGMGAGAARRMAAEGYRVAILSSSGKGEALAKELGGIGVTGSNQSND 60 Query: 61 DLKKAVDACLARWGRIDVLVNSAGHGPKGKVLELSDEEWLLGMEVYLMNVVRSARLVTPL 120 DLK+ VD WGR+D L+NSAGHGP+ VLEL+DEEW GM+VY +NVVR RLVTP+ Sbjct: 61 DLKRLVDTAYDAWGRVDALINSAGHGPRAPVLELTDEEWHRGMDVYFLNVVRPTRLVTPI 120 Query: 121 MQQQGGGAIINISSFSAVEPSQSFPTSSVFRAGLSAYTKLFADKYAPENIRMNNILPGFI 180 M +Q G+I+NIS+++A EP FPTS VFR+GL+A+TKLFAD+YA +NIRMNN+LPGFI Sbjct: 121 MVEQKSGSIVNISTYAAFEPDPLFPTSGVFRSGLAAFTKLFADRYAADNIRMNNVLPGFI 180 Query: 181 NSLPEKPGIKELIPMARYGKVSEVAALASHLASDAAAYITGQNIRIDGGITRSL 234 +SLPE + IPM RYG EVA L + LASD YITGQNIRIDGGITRS+ Sbjct: 181 DSLPETEERRRRIPMGRYGASEEVAELIALLASDRGGYITGQNIRIDGGITRSV 234 >mll1830 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 234 Score = 272 bits (695), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 141/234 (60%), Positives = 182/234 (77%) Query: 1 MSTTKVALVIGGGSGMGAGVAQKLAADGFGIGILSSSGKGESLGNSLGGVGVTGSNRSVA 60 MS KVA+V+ GGSGMGA A++LAADGF + ILSSSGKGE+L LGG+GVTGSN+S Sbjct: 1 MSVEKVAVVVAGGSGMGAAAAKRLAADGFKVAILSSSGKGEALAKELGGLGVTGSNQSSD 60 Query: 61 DLKKAVDACLARWGRIDVLVNSAGHGPKGKVLELSDEEWLLGMEVYLMNVVRSARLVTPL 120 DL++ D L R+GRIDVLVNS GHGP+ +LE++DE+W GM+VYLMNV+R R+V P Sbjct: 61 DLQRLADLTLERFGRIDVLVNSGGHGPRAPILEITDEQWRTGMDVYLMNVIRPVRIVAPQ 120 Query: 121 MQQQGGGAIINISSFSAVEPSQSFPTSSVFRAGLSAYTKLFADKYAPENIRMNNILPGFI 180 M +Q GGAIINIS+ EP+ FPTS+VFRAG++AYTK+F++ YA +N+RMNN+LPG+I Sbjct: 121 MVKQKGGAIINISTAWVAEPNVMFPTSAVFRAGVTAYTKIFSNTYAADNVRMNNVLPGWI 180 Query: 181 NSLPEKPGIKELIPMARYGKVSEVAALASHLASDAAAYITGQNIRIDGGITRSL 234 +SLP ++ +PM RYG EVAA + LAS+ A YITGQNIR+DGG+ R+L Sbjct: 181 DSLPTVEERRDNVPMQRYGTSEEVAATIAFLASEGAGYITGQNIRVDGGVIRAL 234 >DR1943 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Dra Deinococcus radiodurans Length = 254 Score = 122 bits (307), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 89/245 (36%), Positives = 130/245 (53%), Gaps = 22/245 (8%) Query: 5 KVALVIGGGSGMGAGVAQKLAADGFGIGILSSSGKGESLGNSL--------GGVGVTGSN 56 +VALV G G+G +A +LA DGF + + G+GE+ + G V G++ Sbjct: 12 RVALVTGSSRGLGRAMALRLAQDGFTVAV--HYGRGEAEAQQVAADIRAAGGAAQVFGAD 69 Query: 57 RSV-ADLKKAVDACLARWGRIDVLVNSAGHGPKGKVLELSDEEWLLGMEVYLMNVVRSAR 115 S A+ V+ +A GR+DVLVN+AG G + + DE+W ++ L + + R Sbjct: 70 LSQPANAGTLVEDVIAALGRLDVLVNNAGITRDGLAIRMKDEDWDAVLQTNLSSAFAACR 129 Query: 116 LVTPLMQQQGGGAIINISSFSAV--EPSQSFPTSSVFRAGLSAYTKLFADKYAPENIRMN 173 M + G I+N+SS A+ P Q+ +S +AGL T+ A +Y I +N Sbjct: 130 AALKHMMKNRSGRIVNVSSVVALAGNPGQANYVAS--KAGLIGLTRALAKEYGGRGITVN 187 Query: 174 NILPGFINS-----LPE--KPGIKELIPMARYGKVSEVAALASHLASDAAAYITGQNIRI 226 I PGFI S LPE K + IP+AR+G+ EVAAL + LAS+ A YITGQ I + Sbjct: 188 AIAPGFIESDMTAKLPEDTKKQYQANIPLARFGQPEEVAALVAFLASEGAGYITGQTIGV 247 Query: 227 DGGIT 231 DGG+ Sbjct: 248 DGGMN 252