BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0170.p (217 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Rals... 321 7e-88 PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Paste... 240 1e-63 HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemo... 234 8e-62 NMB0590 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Nme Neis... 234 1e-61 NMA0793 [J] COG0336 tRNA-(guanine-N1)-methyltransferase NmA Neis... 230 2e-60 >RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Ralstonia solanacearum Length = 262 Score = 321 bits (823), Expect = 7e-88, Method: Compositional matrix adjust. Identities = 156/215 (72%), Positives = 179/215 (83%), Gaps = 3/215 (1%) Query: 1 LSLWNPRDFTTDNHRTVDDRPYGGGPGMVMLAKPLQQTLQAARQRQLDLNLAAPRVVYLS 60 L WNPRDFTTDN+RTVDDRPYGGGPGMVMLAKPL+ L A R+ Q A VV LS Sbjct: 33 LRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIREAQAP---APSHVVLLS 89 Query: 61 PQGKPLTHQRVMQFAEDTGVVLLCGRYEAIDQRLLDRHVDEEISLGDFVLSGGELPAMAL 120 PQG+PLTH+RVM+ A+ + LLCGRYEAIDQRL+DR VDEEISLGDFVLSGGE+ AMA+ Sbjct: 90 PQGRPLTHRRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAI 149 Query: 121 MDAVIRQLPGVLNDGSSAVEDSFVNGLLDCPHYTRPEEYEGVAVPPVLMGGHHAEIVKWR 180 +DAV+RQLPGVL D SAV+DSFVNGLLDCPHYTRPEEYEGV VP VL+GGHHAEI KWR Sbjct: 150 IDAVVRQLPGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWR 209 Query: 181 RERALEATAKKRPDLIARARQDGLLSKADEKFLSS 215 R++AL T +KRPDLI AR+ GLLS++DE FL++ Sbjct: 210 RQQALLNTMRKRPDLIEAARRQGLLSRSDEVFLAA 244 >PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Pasteurella multocida Length = 245 Score = 240 bits (613), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 113/193 (58%), Positives = 145/193 (75%), Gaps = 4/193 (2%) Query: 4 WNPRDFTTDNHRTVDDRPYGGGPGMVMLAKPLQQTLQAARQRQLDLNLAAPRVVYLSPQG 63 WNPRDFT D H+TVDDRPYGGGPGM+M+ +PL+ +QAA+ + +V+YLSPQG Sbjct: 36 WNPRDFTHDKHKTVDDRPYGGGPGMLMMVQPLRDAIQAAKAEVGE----GAKVIYLSPQG 91 Query: 64 KPLTHQRVMQFAEDTGVVLLCGRYEAIDQRLLDRHVDEEISLGDFVLSGGELPAMALMDA 123 + L V + A+ ++LLCGRYE ID+RL++ VDEE S+GD+VL+GGELPAM L+DA Sbjct: 92 RKLDQAGVKELAQHQKLILLCGRYEGIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDA 151 Query: 124 VIRQLPGVLNDGSSAVEDSFVNGLLDCPHYTRPEEYEGVAVPPVLMGGHHAEIVKWRRER 183 V R +PGVL +SA EDSF GLLDCPHYTRPE +G+AVPPVLM G+H EI KWR + Sbjct: 152 VARFIPGVLGKQASADEDSFAEGLLDCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQ 211 Query: 184 ALEATAKKRPDLI 196 +LE T +RP+L+ Sbjct: 212 SLERTWLRRPELL 224 >HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemophilus influenzae Length = 246 Score = 234 bits (598), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 109/196 (55%), Positives = 144/196 (73%), Gaps = 4/196 (2%) Query: 1 LSLWNPRDFTTDNHRTVDDRPYGGGPGMVMLAKPLQQTLQAARQRQLDLNLAAPRVVYLS 60 + WNPRDFT D H+TVDDRPYGGGPGM+M+ +PL+ + A+ +V+YLS Sbjct: 33 VECWNPRDFTFDKHKTVDDRPYGGGPGMLMMVQPLRDAIHTAKAA----AGEGAKVIYLS 88 Query: 61 PQGKPLTHQRVMQFAEDTGVVLLCGRYEAIDQRLLDRHVDEEISLGDFVLSGGELPAMAL 120 PQG+ L V + A++ ++L+CGRYE ID+RL+ +DEE S+GD+VL+GGELPAM L Sbjct: 89 PQGRKLDQGGVTELAQNQKLILVCGRYEGIDERLIQTEIDEEWSIGDYVLTGGELPAMTL 148 Query: 121 MDAVIRQLPGVLNDGSSAVEDSFVNGLLDCPHYTRPEEYEGVAVPPVLMGGHHAEIVKWR 180 +DAV R +PGVL +SA EDSF +GLLDCPHYTRPE EG+ VPPVLM GHH EI KWR Sbjct: 149 IDAVARFIPGVLGKQASAEEDSFADGLLDCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWR 208 Query: 181 RERALEATAKKRPDLI 196 +++L+ T +RP+L+ Sbjct: 209 LKQSLQRTWLRRPELL 224 >NMB0590 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Nme Neisseria meningitidis MC58 Length = 249 Score = 234 bits (596), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 109/194 (56%), Positives = 146/194 (75%), Gaps = 4/194 (2%) Query: 5 NPRDFTTDNHRTVDDRPYGGGPGMVMLAKPLQQTLQAARQRQLDLNLAAPRVVYLSPQGK 64 NPR F + +DDRP+GGGPGM+M+A PL ++ A+ + + A +V+YLSPQGK Sbjct: 37 NPRKFADNRLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQ----SSQAAKVIYLSPQGK 92 Query: 65 PLTHQRVMQFAEDTGVVLLCGRYEAIDQRLLDRHVDEEISLGDFVLSGGELPAMALMDAV 124 PLTHQ+ ++ AE ++LLCGRYE ID+RLL VDEEIS+GDFV+SGGELPAM LMDAV Sbjct: 93 PLTHQKAVELAELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAV 152 Query: 125 IRQLPGVLNDGSSAVEDSFVNGLLDCPHYTRPEEYEGVAVPPVLMGGHHAEIVKWRRERA 184 +R +PGVL D SA +DSF +G+LDCPHYT+P E++G+AVP VL G+H I +WR E++ Sbjct: 153 LRLVPGVLGDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQS 212 Query: 185 LEATAKKRPDLIAR 198 L T ++RPDL+ + Sbjct: 213 LRRTLERRPDLLEK 226 >NMA0793 [J] COG0336 tRNA-(guanine-N1)-methyltransferase NmA Neisseria meningitidis Z2491 Length = 249 Score = 230 bits (586), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 108/194 (55%), Positives = 144/194 (74%), Gaps = 4/194 (2%) Query: 5 NPRDFTTDNHRTVDDRPYGGGPGMVMLAKPLQQTLQAARQRQLDLNLAAPRVVYLSPQGK 64 NPR F + +DDRP+GGGPGM+M+A PL ++ A+ + + A +V+YLSPQGK Sbjct: 37 NPRKFADNRLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQ----SSQAAKVIYLSPQGK 92 Query: 65 PLTHQRVMQFAEDTGVVLLCGRYEAIDQRLLDRHVDEEISLGDFVLSGGELPAMALMDAV 124 PLTH + + AE ++LLCGRYE ID+RLL VDEEIS+GDFV+SGGELPAM LMDAV Sbjct: 93 PLTHPKAAELAELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAV 152 Query: 125 IRQLPGVLNDGSSAVEDSFVNGLLDCPHYTRPEEYEGVAVPPVLMGGHHAEIVKWRRERA 184 +R +PGVL D SA +DSF +G+LDCPHYT+P E++G+AVP VL G+H I +WR E++ Sbjct: 153 LRLVPGVLGDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQS 212 Query: 185 LEATAKKRPDLIAR 198 L T ++RPDL+ + Sbjct: 213 LRRTLERRPDLLEK 226