BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0152.p (415 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1198 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia s... 556 e-158 YPO2848 [V] COG0841 Cation/multidrug efflux pump Ype Yersinia pe... 517 e-146 yegN [V] COG0841 Cation/multidrug efflux pump Eco Escherichia co... 514 e-146 ZyegN [V] COG0841 Cation/multidrug efflux pump EcZ Escherichia c... 514 e-145 ECs2883 [V] COG0841 Cation/multidrug efflux pump Ecs Escherichia... 514 e-145 >RSp1198 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia solanacearum Length = 1041 Score = 556 bits (1434), Expect = e-158, Method: Compositional matrix adjust. Identities = 271/356 (76%), Positives = 317/356 (89%) Query: 1 MNPSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMT 60 MNPSR+FILRPVAT+LLM+AILL G+VAY+ LPLSALPEVDYPTIQV TLYPGASPDVMT Sbjct: 1 MNPSRIFILRPVATTLLMVAILLSGLVAYRMLPLSALPEVDYPTIQVTTLYPGASPDVMT 60 Query: 61 SSVTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLP 120 SS+TAPLERQFGQMPGL QM+S SSGGASVITLQF+L LSLDIAEQEVQAAINA GNLLP Sbjct: 61 SSITAPLERQFGQMPGLKQMTSASSGGASVITLQFDLSLSLDIAEQEVQAAINAAGNLLP 120 Query: 121 SDLPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGG 180 +DLP PPIYSKVNPAD PILTLA+TSRTLPLP+++D++DTR+AQK+SQL G+GLVS+SGG Sbjct: 121 TDLPMPPIYSKVNPADAPILTLAITSRTLPLPKLEDIVDTRVAQKLSQLPGIGLVSISGG 180 Query: 181 QRPAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADE 240 QRPAVR+Q N +A+AALG+++DDIRTAIGNANVN AKGSFDGP RASTIDANDQL+SA E Sbjct: 181 QRPAVRIQANTQALAALGLSIDDIRTAIGNANVNGAKGSFDGPMRASTIDANDQLRSAAE 240 Query: 241 YRNLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSI 300 Y +I+ YKNGAPIR++DVA ++D AEN +L AWAN + A+I+N+QRQPGANVI VV+ Sbjct: 241 YGTMIVAYKNGAPIRLTDVAQIIDGAENSKLAAWANTTPAIILNVQRQPGANVIDVVNRA 300 Query: 301 KKILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELLLSI 356 K +LP L+ +LP IDV +LTDRTTTIRASV DV+FELLL++ LVVMVI L +I Sbjct: 301 KALLPQLKDTLPANIDVAVLTDRTTTIRASVADVQFELLLAVALVVMVIFLFLRNI 356 >YPO2848 [V] COG0841 Cation/multidrug efflux pump Ype Yersinia pestis Length = 1052 Score = 517 bits (1331), Expect = e-146, Method: Compositional matrix adjust. Identities = 251/351 (71%), Positives = 302/351 (86%) Query: 3 PSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSS 62 PSRLFILRPVAT+L M+AILL GI+ Y+ LP+SALPEVDYPTIQVVTLYPGASPDV+TSS Sbjct: 12 PSRLFILRPVATTLFMIAILLAGIIGYRALPVSALPEVDYPTIQVVTLYPGASPDVVTSS 71 Query: 63 VTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 +TAPLERQFGQM GL QM+S SSGGASVITLQF L L LD+AEQEVQAAINA NLLPSD Sbjct: 72 ITAPLERQFGQMSGLKQMASQSSGGASVITLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131 Query: 123 LPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGGQR 182 LP PPIY+KVNPAD PILTLAVT+ +P+ +V+D+++TR+AQKISQ++GVGLV+LSGGQR Sbjct: 132 LPYPPIYNKVNPADPPILTLAVTATAIPMTQVEDMVETRIAQKISQVTGVGLVTLSGGQR 191 Query: 183 PAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADEYR 242 PAVR++LN A+AALG++ + IRTAI NANVN AKGS DGP R+ T+ ANDQ+KSA+EYR Sbjct: 192 PAVRVKLNAPAVAALGLDSETIRTAISNANVNSAKGSLDGPTRSVTLSANDQMKSAEEYR 251 Query: 243 NLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSIKK 302 +LII Y+NGAPIR+ DVA + AEN +L AWAN +A+++NIQRQPG NVIA DSI++ Sbjct: 252 DLIIAYQNGAPIRLQDVATIEQGAENNKLAAWANTQSAIVLNIQRQPGVNVIATADSIRE 311 Query: 303 ILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELL 353 +LP L SLP ++DV +LTDRT+TIRASV+DV+FELLL+I LVVMVI L Sbjct: 312 MLPELIKSLPKSVDVKVLTDRTSTIRASVNDVQFELLLAIALVVMVIYLFL 362 >yegN [V] COG0841 Cation/multidrug efflux pump Eco Escherichia coli K12 Length = 1040 Score = 514 bits (1325), Expect = e-146, Method: Compositional matrix adjust. Identities = 249/354 (70%), Positives = 301/354 (85%) Query: 3 PSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSS 62 PSRLFI+RPVAT+LLM+AILL GI+ Y+ LP+SALPEVDYPTIQVVTLYPGASPDVMTS+ Sbjct: 12 PSRLFIMRPVATTLLMVAILLAGIIGYRALPVSALPEVDYPTIQVVTLYPGASPDVMTSA 71 Query: 63 VTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 VTAPLERQFGQM GL QMSS SSGGASVITLQF L L LD+AEQEVQAAINA NLLPSD Sbjct: 72 VTAPLERQFGQMSGLKQMSSQSSGGASVITLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131 Query: 123 LPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGGQR 182 LP PP+YSKVNPAD PI+TLAVTS +P+ +V+D+++TR+AQKISQ+SGVGLV+LSGGQR Sbjct: 132 LPNPPVYSKVNPADPPIMTLAVTSTAMPMTQVEDMVETRVAQKISQISGVGLVTLSGGQR 191 Query: 183 PAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADEYR 242 PAVR++LN +AIAALG+ + +RTAI ANVN AKGS DGP+RA T+ ANDQ++SA+EYR Sbjct: 192 PAVRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGPSRAVTLSANDQMQSAEEYR 251 Query: 243 NLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSIKK 302 LII Y+NGAPIR+ DVA V AEN LGAWAN A+++N+QRQPGAN+I+ DSI++ Sbjct: 252 QLIIAYQNGAPIRLGDVATVEQGAENSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQ 311 Query: 303 ILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELLLSI 356 +LP L SLP ++ V +L+DRTT IRASVDD +FEL+++I LVVM+I L +I Sbjct: 312 MLPQLTESLPKSVKVTVLSDRTTNIRASVDDTQFELMMAIALVVMIIYLFLRNI 365 >ZyegN [V] COG0841 Cation/multidrug efflux pump EcZ Escherichia coli O157:H7 EDL933 Length = 1040 Score = 514 bits (1325), Expect = e-145, Method: Compositional matrix adjust. Identities = 249/354 (70%), Positives = 301/354 (85%) Query: 3 PSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSS 62 PSRLFI+RPVAT+LLM+AILL GI+ Y+ LP+SALPEVDYPTIQVVTLYPGASPDVMTS+ Sbjct: 12 PSRLFIMRPVATTLLMVAILLAGIIGYRALPVSALPEVDYPTIQVVTLYPGASPDVMTSA 71 Query: 63 VTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 VTAPLERQFGQM GL QMSS SSGGASVITLQF L L LD+AEQEVQAAINA NLLPSD Sbjct: 72 VTAPLERQFGQMSGLKQMSSQSSGGASVITLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131 Query: 123 LPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGGQR 182 LP PP+YSKVNPAD PI+TLAVTS +P+ +V+D+++TR+AQKISQ+SGVGLV+LSGGQR Sbjct: 132 LPNPPVYSKVNPADPPIMTLAVTSTAMPMTQVEDMVETRVAQKISQISGVGLVTLSGGQR 191 Query: 183 PAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADEYR 242 PAVR++LN +AIAALG+ + +RTAI ANVN AKGS DGP+RA T+ ANDQ++SA+EYR Sbjct: 192 PAVRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGPSRAVTLSANDQMQSAEEYR 251 Query: 243 NLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSIKK 302 LII Y+NGAPIR+ DVA V AEN LGAWAN A+++N+QRQPGAN+I+ DSI++ Sbjct: 252 QLIIAYQNGAPIRLGDVATVEQGAENSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQ 311 Query: 303 ILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELLLSI 356 +LP L SLP ++ V +L+DRTT IRASVDD +FEL+++I LVVM+I L +I Sbjct: 312 MLPQLTESLPKSVKVTVLSDRTTNIRASVDDTQFELMMAIALVVMIIYLFLRNI 365 >ECs2883 [V] COG0841 Cation/multidrug efflux pump Ecs Escherichia coli O157:H7 Length = 1040 Score = 514 bits (1325), Expect = e-145, Method: Compositional matrix adjust. Identities = 249/354 (70%), Positives = 301/354 (85%) Query: 3 PSRLFILRPVATSLLMLAILLVGIVAYKQLPLSALPEVDYPTIQVVTLYPGASPDVMTSS 62 PSRLFI+RPVAT+LLM+AILL GI+ Y+ LP+SALPEVDYPTIQVVTLYPGASPDVMTS+ Sbjct: 12 PSRLFIMRPVATTLLMVAILLAGIIGYRALPVSALPEVDYPTIQVVTLYPGASPDVMTSA 71 Query: 63 VTAPLERQFGQMPGLNQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGGNLLPSD 122 VTAPLERQFGQM GL QMSS SSGGASVITLQF L L LD+AEQEVQAAINA NLLPSD Sbjct: 72 VTAPLERQFGQMSGLKQMSSQSSGGASVITLQFQLTLPLDVAEQEVQAAINAATNLLPSD 131 Query: 123 LPAPPIYSKVNPADTPILTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSGGQR 182 LP PP+YSKVNPAD PI+TLAVTS +P+ +V+D+++TR+AQKISQ+SGVGLV+LSGGQR Sbjct: 132 LPNPPVYSKVNPADPPIMTLAVTSTAMPMTQVEDMVETRVAQKISQISGVGLVTLSGGQR 191 Query: 183 PAVRMQLNPRAIAALGMNLDDIRTAIGNANVNQAKGSFDGPARASTIDANDQLKSADEYR 242 PAVR++LN +AIAALG+ + +RTAI ANVN AKGS DGP+RA T+ ANDQ++SA+EYR Sbjct: 192 PAVRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSLDGPSRAVTLSANDQMQSAEEYR 251 Query: 243 NLIIGYKNGAPIRVSDVADVVDAAENVRLGAWANDSAAVIVNIQRQPGANVIAVVDSIKK 302 LII Y+NGAPIR+ DVA V AEN LGAWAN A+++N+QRQPGAN+I+ DSI++ Sbjct: 252 QLIIAYQNGAPIRLGDVATVEQGAENSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQ 311 Query: 303 ILPTLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSIVLVVMVIXELLLSI 356 +LP L SLP ++ V +L+DRTT IRASVDD +FEL+++I LVVM+I L +I Sbjct: 312 MLPQLTESLPKSVKVTVLSDRTTNIRASVDDTQFELMMAIALVVMIIYLFLRNI 365