BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0146.p (657 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3506 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 622 e-178 MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 114 8e-25 aq_451 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 107 8e-23 MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 107 1e-22 PM1628 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac... 107 1e-22 >PA3506 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Pae Pseudomonas aeruginosa Length = 560 Score = 622 bits (1605), Expect = e-178, Method: Compositional matrix adjust. Identities = 315/397 (79%), Positives = 348/397 (87%), Gaps = 3/397 (0%) Query: 1 MVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTI 60 +VEALTAGTPLLHLTGQIETPYLD+SLAYIHEAPDQLSMLKAISKAAFR+RS +TAIST+ Sbjct: 89 LVEALTAGTPLLHLTGQIETPYLDRSLAYIHEAPDQLSMLKAISKAAFRVRSVDTAISTL 148 Query: 61 KLAVQTALTPPMGPVSVEIPIDIQAALIPMPSDLRPLPIAVQAPSEHALDELAERLLKAG 120 KLAVQTALT P GPVSVEIPIDIQ+ALI +P+DL PLP+ V PSE ALD+LAER +A Sbjct: 149 KLAVQTALTAPAGPVSVEIPIDIQSALINLPADLSPLPVPVARPSEQALDDLAERFAQAR 208 Query: 121 RPLLWLGGGARRAGKQVQRLLDLGFGVVTSTQGRGIVAEDDPRSLGSFNLHKPVENFYQT 180 RP+LWLGGGAR AG QVQRLL +G GVVTSTQGRGIV EDD RSLG+FNL+KPVE FYQ+ Sbjct: 209 RPMLWLGGGARHAGPQVQRLLAMGVGVVTSTQGRGIVPEDDARSLGAFNLNKPVERFYQS 268 Query: 181 CDAIVVIGSRLRGNETLKYELKLPRPLYRIDADPSAEGRCYNSDHFVCGDAALALEGLAD 240 CDA++V+GSRLRGNETLKYELKLPRPLYR DAD +AEGRCY SD FVCGD+ L+GLAD Sbjct: 269 CDAMLVVGSRLRGNETLKYELKLPRPLYRADADATAEGRCYPSDFFVCGDSERVLKGLAD 328 Query: 241 RLEKRKSAIHIDPAFMADLKKARDAAVAGLVDGLGPYSALVEQVQKAAGRKFNWVRDVTV 300 RLE R S +DPAF ADL AR+ A A LVDGLGPY++LVEQ+Q AGR FNWVRDVTV Sbjct: 329 RLEGRLS---VDPAFAADLAAARNQARAQLVDGLGPYASLVEQLQALAGRNFNWVRDVTV 385 Query: 301 SNSTWGNRYLRIFDPLAGVHALGGGIGQGLAMGIGAAIGAAVTASGKKTFTLAGDGGFIL 360 SNSTWGNR+L IF P AGVHALGGGIGQGLAMGIGAA+GAA TA G+KTF LAGDGGFIL Sbjct: 386 SNSTWGNRHLNIFSPRAGVHALGGGIGQGLAMGIGAAVGAAETAPGRKTFVLAGDGGFIL 445 Query: 361 NLGELATAVQEKADMVIVLMNDKGYGVIKNIQDVQRG 397 NLGELATAVQE+ADM+IVLMND+GYGVIKNIQD G Sbjct: 446 NLGELATAVQERADMLIVLMNDQGYGVIKNIQDAAYG 482 Score = 76.3 bits (186), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 36/64 (56%), Positives = 45/64 (70%) Query: 579 QLSQALSLRHTRVSNLADVEGALKTALSESGPFLLEIDMLAIGSFKTAFAGPPVTEKAAA 638 QLS +L LRH +VS+L D L AL+E GPFLLE+DMLA+GSFK+ FAGPP + Sbjct: 497 QLSASLGLRHAKVSDLKDFSAVLDGALAEPGPFLLEVDMLAVGSFKSIFAGPPTNDAKTD 556 Query: 639 EAAA 642 +A A Sbjct: 557 KATA 560 >MK0531 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Mka Methanopyrus kandleri AV19 Length = 565 Score = 114 bits (285), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 112/408 (27%), Positives = 190/408 (46%), Gaps = 42/408 (10%) Query: 9 TPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTIKLAVQTAL 68 +P++ +TGQ+ T + + + D + + I+K ++I E +K A + A+ Sbjct: 95 SPVIAITGQVPTTMIGKDA---FQEVDAVGVFMPITKHNYQIGKPEEIPEVVKEAFKIAI 151 Query: 69 TPPMGPVSVEIPIDIQAAL----IPMPSDLRPLPIAVQAPSEHALDELAERLLKAGRPLL 124 T GPV +++P D+Q A IP ++ L + V+ + AE L +A RP++ Sbjct: 152 TGRPGPVHIDVPKDVQEAEVDVEIPKTVEVEGLNV-VKRGHPVQIKRAAELLAEAERPVI 210 Query: 125 WLGGGA--RRAGKQVQRLLDL-GFGVVTSTQGRGIVAEDDPRSLGSFNLH-KPVENFYQT 180 GGG A +++ L +L G V T+ G+G ED P +LG +H N+ T Sbjct: 211 LAGGGCVISNATRELIELAELLGAPVATTLMGKGAFPEDHPLALGMAGMHGTKAANYALT 270 Query: 181 -CDAIVVIGSRLRGNETLKYELKLPR-PLYRIDADPSAEGRCYNSDHFVCGDAALALEGL 238 CD ++ +G R T P + ID DP+ G+ D + GDA L L L Sbjct: 271 ECDVLLAVGCRFSDRTTGDPSGFAPEAKIIHIDIDPAEIGKNIPVDVPIVGDAKLVLRDL 330 Query: 239 ADRLEKRKSAIHIDPAFMADLKK--ARDAAVAGLVDGLGPYSALVEQVQKAAGRKFNWV- 295 L++RK ++ + K+ R + V+ + P S E Q+ + R+ V Sbjct: 331 IKELKRRK--------YLRERKRWGERIEELKAEVE-MPPEST--ESDQRISPRELVRVL 379 Query: 296 ------RDVTVS-----NSTWGNRYLRIFDPLAGVHALGGGIGQGLAMGIGAAIGAAVTA 344 RD ++ N W RY + +P + + GG+G + G+ AA+GA V A Sbjct: 380 HEALKDRDYILTTDVGQNQMWMARYFPVEEPRRFISS--GGLGT-MGFGLPAALGAKVAA 436 Query: 345 SGKKTFTLAGDGGFILNLGELATAVQEKADMVIVLMNDKGYGVIKNIQ 392 K + GDGGF++ ELATAV ++ + +M+++ G++ Q Sbjct: 437 PEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMVAQWQ 484 >aq_451 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Aae Aquifex aeolicus Length = 585 Score = 107 bits (267), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 108/419 (25%), Positives = 199/419 (47%), Gaps = 39/419 (9%) Query: 1 MVEALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTI 60 + +A PL+ +TGQ+ T +L + A+ + D + + + I+K F ++S E + Sbjct: 86 IADAYMDSVPLVAITGQVPT-HLIGNDAF--QEVDIVGITRPITKHNFLVKSIEELPLIL 142 Query: 61 KLAVQTALTPPMGPVSVEIPIDI--QAALIPMPSDLRPLPIAVQAPSEHA------LDEL 112 + A A T GPV V++P D+ Q A + +PSD + A+ H + + Sbjct: 143 REAFYIARTGRPGPVLVDLPKDVTQQIADVKIPSD-EEVKAALPGYKPHVEGNPQQIKKA 201 Query: 113 AERLLKAGRPLLWLGGGARRAGKQVQRLLDLG----FGVVTSTQGRGIVAEDDPRSLGSF 168 AE ++ A RP+L++GGGA + Q + L++L V T+TQG+G E P SL Sbjct: 202 AELIMSAKRPVLYVGGGAVNSEAQ-EELIELAELMQIPVTTTTQGKGAFPETHPLSLRML 260 Query: 169 NLHKPVENFY-----QTCDAIVVIGSRLRGNETLKYELKLP-RPLYRIDADPSAEGRCYN 222 +H +Y D ++ +G+R T K + P + ID DP++ + Sbjct: 261 GMHG---TYYANMAVYNSDLLIAVGARFDDRVTGKIDEFAPGAKIIHIDIDPASISKNIT 317 Query: 223 SDHFVCGDAALALEGLADRLEKRKSAIHIDP----AFMADLKKARD--AAVAGLVDGLGP 276 D + GD + L L + ++KR + I + P ++ ++K + D + Sbjct: 318 VDVPIVGDVKIVLRKLINEIKKRGAKI-LYPEERKKWLEQIEKWKKLHPLTYRNSDKVIK 376 Query: 277 YSALVEQVQKAAGRKFNWVRDVTVSNSTWGNRYLRIFDPLAGVHALG-GGIGQGLAMGIG 335 ++EQ+ +A + V V + W + + P +++ G G +G GL GIG Sbjct: 377 PQYVIEQLWEATNGEAIIVPGVG-QHQMWAAMFYKFKYPRQFINSGGLGTMGFGLPAGIG 435 Query: 336 AAIGAAVTASGKKTFTLAGDGGFILNLGELATAVQEKADMVIVLMNDKGYGVIKNIQDV 394 A IG ++ + + GDG F++ + E+ TAVQ K + + ++N+ G+++ Q++ Sbjct: 436 AKIGR----PDREVWVVDGDGSFVMTMQEVITAVQYKVPVKVAIINNAYLGMVRQWQEL 490 >MJ0277 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Mja Methanococcus jannaschii Length = 591 Score = 107 bits (266), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 106/415 (25%), Positives = 184/415 (44%), Gaps = 31/415 (7%) Query: 4 ALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTIKLA 63 A +P++ LTGQ+ T + + D L + I+K F+I+ E T + A Sbjct: 85 AYADSSPVIALTGQVPTKLIGNDA---FQEIDALGLFMPITKHNFQIKKPEEIPETFRAA 141 Query: 64 VQTALTPPMGPVSVEIPIDIQAALIPMPSDLRPLPIAVQAPSEHA--------LDELAER 115 + A T GPV +++P D+Q I + + P+P V P + + A+ Sbjct: 142 FEIATTGRPGPVHIDLPKDVQDGEIDI--EKYPIPAKVDLPGYKPKTVGHPLQIKKAAKL 199 Query: 116 LLKAGRPLLWLGGGARRAG--KQVQRLLD-LGFGVVTSTQGRGIVAEDDPRSLGSFNLH- 171 + ++ RP++ GGG +G +++ RL + + V T+ G+G ED P +LG +H Sbjct: 200 IAESERPVILAGGGVIISGASEELLRLAEFVKIPVCTTLMGKGCFPEDHPLALGMVGMHG 259 Query: 172 KPVENFYQT-CDAIVVIGSRLRGNETLKYELKLPR-PLYRIDADPSAEGRCYNSDHFVCG 229 N+ T CD ++ IG R T P + ID DP+ G+ +D + G Sbjct: 260 TKAANYAVTECDVLIAIGCRFSDRVTGDIRYFAPEAKIIHIDIDPAEIGKNVRADIPIVG 319 Query: 230 DAALALEGLAD---RLEKRKSAIHIDPAFMADLKKARDAAVAGLVDGLGP---YSALVEQ 283 DA L L LE + ++ + +LKK + + P L+E Sbjct: 320 DAKNVLRDLLAALIALEIKDKETWLERIY--ELKKLSIPMMDFDDKPIKPQRFVKDLMEV 377 Query: 284 VQKAAGRKFNWVRDVTV-SNSTWGNRYLRIFDPLAGVHALGGGIGQGLAMGIGAAIGAAV 342 + + + N + V N W + + P + + + GG+G + G AAIGA V Sbjct: 378 LNEIDSKLKNTIITTDVGQNQMWMAHFFKTKMPRSFLAS--GGLGT-MGFGFPAAIGAKV 434 Query: 343 TASGKKTFTLAGDGGFILNLGELATAVQEKADMVIVLMNDKGYGVIKNIQDVQRG 397 ++ GDGGF++N ELAT + +VI + +++ G++ Q++ G Sbjct: 435 AKPYANVISITGDGGFLMNSQELATISEYDIPVVICIFDNRTLGMVYQWQNLYYG 489 >PM1628 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] Pmu Pasteurella multocida Length = 549 Score = 107 bits (266), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 105/406 (25%), Positives = 184/406 (45%), Gaps = 34/406 (8%) Query: 3 EALTAGTPLLHLTGQIETPYLDQSLAYIHEAPDQLSMLKAISKAAFRIRSTETAISTIKL 62 +AL P++ +TGQ+ +P + EA D L + A +K +F ++S E I Sbjct: 84 DALMDSIPVVAITGQVASPLIGTDA--FQEA-DVLGLSLACTKHSFLVQSIEELPDVIAK 140 Query: 63 AVQTALTPPMGPVSVEIPIDIQAALIPMPSDLRPLPIAVQAP---SEHALDELAERLLKA 119 A + A + GPV ++IP DIQ A P+D+ P +V P S+ L + L +A Sbjct: 141 AFEIAASGRPGPVLIDIPKDIQIA----PTDVTPFTYSVAKPTALSQAHLTDALTLLKQA 196 Query: 120 GRPLLWLGGGA--RRAGKQVQRLLDLG-FGVVTSTQGRGIVAEDDPRSLGSFNLH--KPV 174 RP+L++GGG +A ++ L + V++ +G G V D+P +G +H K Sbjct: 197 KRPVLYVGGGVGMAQAVPALREFLAVTQMPSVSTLKGLGSVHPDNPYYMGMIGMHGTKAA 256 Query: 175 ENFYQTCDAIVVIGSRLRGNETLKYELKLPR-PLYRIDADPSAEGRCYNSDHFVCGDAAL 233 Q CD ++ G+R T K + P + D D + G+ D +CGD Sbjct: 257 NYAVQECDLLLAFGARFDDRVTGKLDTFAPHAKVIHADIDMAEIGKLRRPDVALCGDIVQ 316 Query: 234 ALEGLADRLEKRKSAIHIDPAFMADLKKARDAAVAG-----LVDGLGPYSALVEQVQKAA 288 A L+ L + HI LK+ D + ++ L L+ + K Sbjct: 317 AFNALSIPLNIAEWQAHIK-----QLKQTHDFCYSTNQGDTFINPLW----LLNSLSKKK 367 Query: 289 GRKFNWVRDVTVSNSTWGNRYLRIFDPLAGVHALGGGIGQGLAMGIGAAIGAAVTASGKK 348 + DV + W ++++ + P + + G G + G+ AAIGA + Sbjct: 368 PQSAVITTDVG-QHQMWSAQHMQHYAPENYITSAGYGT---MGFGLPAAIGAKKARPDDE 423 Query: 349 TFTLAGDGGFILNLGELATAVQEKADMVIVLMNDKGYGVIKNIQDV 394 ++GDG ++N+ EL T + K + IVL++++ G+++ Q++ Sbjct: 424 VILISGDGSIMMNIQELGTFKRGKTPVKIVLLDNQRLGMVRQWQEL 469