BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0140.p (538 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1199 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia s... 325 2e-88 STM2128 [V] COG0841 Cation/multidrug efflux pump Sty Salmonella ... 265 1e-70 YPO2849 [V] COG0841 Cation/multidrug efflux pump Ype Yersinia pe... 263 7e-70 ZyegO [V] COG0841 Cation/multidrug efflux pump EcZ Escherichia c... 261 3e-69 yegO [V] COG0841 Cation/multidrug efflux pump Eco Escherichia co... 261 3e-69 >RSp1199 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia solanacearum Length = 1033 Score = 325 bits (832), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 165/289 (57%), Positives = 201/289 (69%), Gaps = 38/289 (13%) Query: 3 GASLFLQAVQDIRVGARQSDSQYEYTLQSDDLELLRTWEPKIRVAFSNLPQLEDVSTDQQ 62 GA+LFLQAVQDIR+G R S++QY+YTLQ+D+LE LR WEPKIR A + + L DV+TD Q Sbjct: 640 GANLFLQAVQDIRIGGRPSNAQYQYTLQADNLEELRAWEPKIRQALTQIKSLVDVNTDAQ 699 Query: 63 DKGLQTTLTIDRETAIRSGVTPQLIDTSLSDLFGQRQISTIYSGMNQYHVVMEAAPQYWQ 122 DKG QT L DR+ A+ G+TP+ ID +L++ FGQRQ+STIY+ +NQY VVMEAAPQYWQ Sbjct: 700 DKGQQTALVFDRDKAMALGITPKDIDNALNNAFGQRQVSTIYNPLNQYRVVMEAAPQYWQ 759 Query: 123 SPEILNSTYVSLANSTANLSPTTSALGTPPALTAGGKLNSNSSLALTLSTAASKQVPLTS 182 +PE L Y L TA+ QVPL++ Sbjct: 760 NPEGLRDIY--------------------------------------LITASGAQVPLST 781 Query: 183 FSSFAPTTTSLSVSHEGQFIASTISFNLAEGVSLSQATQVINDTLARLGVPTAVHGAFAG 242 FS + PT T L+V+H+GQF AST+SFNLA G SLS ATQ I D + R+GVPT VHG+F G Sbjct: 782 FSHYGPTNTPLAVNHQGQFAASTLSFNLAPGASLSAATQQIQDAMNRIGVPTTVHGSFQG 841 Query: 243 TANAFQSSLDSQPVLILTALLAVYIVLGILYESLVHPITIISTLPSAGV 291 TA AFQ SL SQPVLIL AL+ +YIVLG+LYES VHPITI+STLPSAGV Sbjct: 842 TARAFQDSLSSQPVLILFALITIYIVLGVLYESYVHPITILSTLPSAGV 890 >STM2128 [V] COG0841 Cation/multidrug efflux pump Sty Salmonella typhimurium LT2 Length = 1026 Score = 265 bits (678), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 143/289 (49%), Positives = 181/289 (62%), Gaps = 38/289 (13%) Query: 3 GASLFLQAVQDIRVGARQSDSQYEYTLQSDDLELLRTWEPKIRVAFSNLPQLEDVSTDQQ 62 GA LFL AVQDIRVG RQ+++ Y+YTL SD L LR WEPKIR A S LPQL DV++DQQ Sbjct: 639 GARLFLMAVQDIRVGGRQANASYQYTLLSDSLAALREWEPKIRKALSALPQLADVNSDQQ 698 Query: 63 DKGLQTTLTIDRETAIRSGVTPQLIDTSLSDLFGQRQISTIYSGMNQYHVVMEAAPQYWQ 122 D G + L DR+T R G+ Q ++ L++ FGQRQISTIY MNQY VVME P+Y Q Sbjct: 699 DNGAEMNLIYDRDTMSRLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYSQ 758 Query: 123 SPEILNSTYVSLANSTANLSPTTSALGTPPALTAGGKLNSNSSLALTLSTAASKQVPLTS 182 L +V +N + K +PL+ Sbjct: 759 DISALEKMFV---------------------------INRD-----------GKAIPLSY 780 Query: 183 FSSFAPTTTSLSVSHEGQFIASTISFNLAEGVSLSQATQVINDTLARLGVPTAVHGAFAG 242 F+ + P LSV+H+G ASTI+FNL G SLSQAT+ IN T+ +LGVP+ V G+F+G Sbjct: 781 FAQWRPANAPLSVNHQGLSAASTIAFNLPTGTSLSQATEAINRTMTQLGVPSTVRGSFSG 840 Query: 243 TANAFQSSLDSQPVLILTALLAVYIVLGILYESLVHPITIISTLPSAGV 291 TA FQ +++SQ +LI+ A+ VYIVLGILYES VHP+TI+STLPSAGV Sbjct: 841 TAQVFQQTMNSQLILIVAAIATVYIVLGILYESYVHPLTILSTLPSAGV 889 >YPO2849 [V] COG0841 Cation/multidrug efflux pump Ype Yersinia pestis Length = 1024 Score = 263 bits (672), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 141/289 (48%), Positives = 180/289 (62%), Gaps = 38/289 (13%) Query: 3 GASLFLQAVQDIRVGARQSDSQYEYTLQSDDLELLRTWEPKIRVAFSNLPQLEDVSTDQQ 62 GA+LFL +VQDIRVG R S++ Y++TL +DDL LR WEPK+R A + LPQL DV++DQQ Sbjct: 639 GANLFLSSVQDIRVGGRHSNAAYQFTLLADDLAALREWEPKVRAALAKLPQLADVNSDQQ 698 Query: 63 DKGLQTTLTIDRETAIRSGVTPQLIDTSLSDLFGQRQISTIYSGMNQYHVVMEAAPQYWQ 122 DKG + LT DRET R G+ + L++ FGQRQISTIY +NQY VVME AP+Y Q Sbjct: 699 DKGAEMALTYDRETMARLGIDVSEANALLNNAFGQRQISTIYQPLNQYKVVMEVAPEYTQ 758 Query: 123 SPEILNSTYVSLANSTANLSPTTSALGTPPALTAGGKLNSNSSLALTLSTAASKQVPLTS 182 L+ +V +NSN + +PL+ Sbjct: 759 DVSSLDKMFV---------------------------INSN-----------GQSIPLSY 780 Query: 183 FSSFAPTTTSLSVSHEGQFIASTISFNLAEGVSLSQATQVINDTLARLGVPTAVHGAFAG 242 F+ + P L+V+H+G ASTISFNL +G SLS+AT + + LGVP+ V GAFAG Sbjct: 781 FAKWQPANAPLAVNHQGLSAASTISFNLPDGGSLSEATAAVERAMTELGVPSTVRGAFAG 840 Query: 243 TANAFQSSLDSQPVLILTALLAVYIVLGILYESLVHPITIISTLPSAGV 291 TA FQ +L SQ LI+ A+ VYIVLGILYES VHP+TI+STLPSAGV Sbjct: 841 TAQVFQETLKSQLWLIMAAIATVYIVLGILYESYVHPLTILSTLPSAGV 889 >ZyegO [V] COG0841 Cation/multidrug efflux pump EcZ Escherichia coli O157:H7 EDL933 Length = 1025 Score = 261 bits (668), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 140/289 (48%), Positives = 179/289 (61%), Gaps = 38/289 (13%) Query: 3 GASLFLQAVQDIRVGARQSDSQYEYTLQSDDLELLRTWEPKIRVAFSNLPQLEDVSTDQQ 62 GA+LFL AVQDIRVG RQS++ Y+YTL SDDL LR WEPKIR + LP+L DV++DQQ Sbjct: 639 GANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLATLPELADVNSDQQ 698 Query: 63 DKGLQTTLTIDRETAIRSGVTPQLIDTSLSDLFGQRQISTIYSGMNQYHVVMEAAPQYWQ 122 D G + L DR+T R G+ Q ++ L++ FGQRQISTIY MNQY VVME P+Y Q Sbjct: 699 DNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQ 758 Query: 123 SPEILNSTYVSLANSTANLSPTTSALGTPPALTAGGKLNSNSSLALTLSTAASKQVPLTS 182 L +V +N+ K +PL+ Sbjct: 759 DISALEKMFV---------------------------INNE-----------GKAIPLSY 780 Query: 183 FSSFAPTTTSLSVSHEGQFIASTISFNLAEGVSLSQATQVINDTLARLGVPTAVHGAFAG 242 F+ + P LSV+H+G ASTISFNL G SLS A+ I+ + +LGVP+ V G+FAG Sbjct: 781 FAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAG 840 Query: 243 TANAFQSSLDSQPVLILTALLAVYIVLGILYESLVHPITIISTLPSAGV 291 TA FQ +++SQ +LI+ A+ VYIVLGILYES VHP+TI+STLPSAGV Sbjct: 841 TAQVFQETMNSQVILIIAAIATVYIVLGILYESYVHPLTILSTLPSAGV 889 >yegO [V] COG0841 Cation/multidrug efflux pump Eco Escherichia coli K12 Length = 1025 Score = 261 bits (667), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 140/289 (48%), Positives = 179/289 (61%), Gaps = 38/289 (13%) Query: 3 GASLFLQAVQDIRVGARQSDSQYEYTLQSDDLELLRTWEPKIRVAFSNLPQLEDVSTDQQ 62 GA+LFL AVQDIRVG RQS++ Y+YTL SDDL LR WEPKIR + LP+L DV++DQQ Sbjct: 639 GANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLATLPELADVNSDQQ 698 Query: 63 DKGLQTTLTIDRETAIRSGVTPQLIDTSLSDLFGQRQISTIYSGMNQYHVVMEAAPQYWQ 122 D G + L DR+T R G+ Q ++ L++ FGQRQISTIY MNQY VVME P+Y Q Sbjct: 699 DNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMNQYKVVMEVDPRYTQ 758 Query: 123 SPEILNSTYVSLANSTANLSPTTSALGTPPALTAGGKLNSNSSLALTLSTAASKQVPLTS 182 L +V +N+ K +PL+ Sbjct: 759 DISALEKMFV---------------------------INNE-----------GKAIPLSY 780 Query: 183 FSSFAPTTTSLSVSHEGQFIASTISFNLAEGVSLSQATQVINDTLARLGVPTAVHGAFAG 242 F+ + P LSV+H+G ASTISFNL G SLS A+ I+ + +LGVP+ V G+FAG Sbjct: 781 FAKWQPANAPLSVNHQGLSAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAG 840 Query: 243 TANAFQSSLDSQPVLILTALLAVYIVLGILYESLVHPITIISTLPSAGV 291 TA FQ +++SQ +LI+ A+ VYIVLGILYES VHP+TI+STLPSAGV Sbjct: 841 TAQVFQETMNSQVILIIAAIATVYIVLGILYESYVHPLTILSTLPSAGV 889