BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0089.p (326 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1952 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 588 e-168 PM0692 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 454 e-127 sll0542 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 389 e-108 DR2471 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ... 372 e-103 Cj1537c [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid... 360 3e-99 >RSc1952 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Rso Ralstonia solanacearum Length = 660 Score = 588 bits (1515), Expect = e-168, Method: Compositional matrix adjust. Identities = 276/326 (84%), Positives = 302/326 (92%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 VFEGVPTYPNAGRFWDM+Q+H+ FYTAPTAIRSLIKAAEADE HPK+YDLSSLRLLG Sbjct: 335 VFEGVPTYPNAGRFWDMIQKHRVNTFYTAPTAIRSLIKAAEADETSHPKRYDLSSLRLLG 394 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 +VGEPINPEAWMWY+ NIG +CPIVDTFWQTETGGHMISPLPGATP VPGSCTLPLPGI Sbjct: 395 TVGEPINPEAWMWYHTNIGGGRCPIVDTFWQTETGGHMISPLPGATPLVPGSCTLPLPGI 454 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGDGA 180 AAIVDETG DLP+G GGILVVKRPWP+MIRTIW DPERF+KSYFP E GGK+YLAGDG+ Sbjct: 455 MAAIVDETGQDLPDGQGGILVVKRPWPAMIRTIWGDPERFRKSYFPAELGGKLYLAGDGS 514 Query: 181 IRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICA 240 +R+K+TGYFTI GRIDDVLNVSGHRMGTMEIESALVANP+VAEAAVVG+PD+ TGE+ICA Sbjct: 515 VRDKETGYFTIMGRIDDVLNVSGHRMGTMEIESALVANPLVAEAAVVGRPDDMTGEAICA 574 Query: 241 FVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL 300 FVVLKR RP GDEA+ IA +LR+WV KEIGPIAKPK+IRFGDNLPKTRSGKIMRRLLR + Sbjct: 575 FVVLKRTRPNGDEARQIATDLRNWVGKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSI 634 Query: 301 AKGEDITQDISTLENPAILDQLKEAQ 326 AKGEDITQD STLENPAIL+QLKEA+ Sbjct: 635 AKGEDITQDTSTLENPAILEQLKEAR 660 >PM0692 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Pmu Pasteurella multocida Length = 653 Score = 454 bits (1168), Expect = e-127, Method: Compositional matrix adjust. Identities = 217/324 (66%), Positives = 260/324 (80%), Gaps = 5/324 (1%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 +FEGVPTYP+ GR W M+QRHK +FYT+PT IRSL + + P +YDLSSLRLLG Sbjct: 332 IFEGVPTYPDPGRIWRMIQRHKINVFYTSPTLIRSLTRLGDH----IPNKYDLSSLRLLG 387 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 SVGEPINP AWMW+Y+ +G+ +CPIVDT+WQTETG M++P+PG T PGSCTLPLPGI Sbjct: 388 SVGEPINPSAWMWFYEVVGKSRCPIVDTWWQTETGSIMLAPIPGVTATKPGSCTLPLPGI 447 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGDGA 180 A ++DE G GG L +KRP+PSM+RTIWNDPER+K +YFP E+GGK Y+AGD A Sbjct: 448 MAEVLDENGQKCAVEQGGALAIKRPFPSMLRTIWNDPERYKSTYFPAEYGGKYYIAGDNA 507 Query: 181 IRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICA 240 R+KD GYF I GR DDVLNVSGHR+GTMEIESALVA+P VAEAAVVGKPDE GE+I A Sbjct: 508 HRDKD-GYFWILGRTDDVLNVSGHRLGTMEIESALVASPKVAEAAVVGKPDEIKGEAIVA 566 Query: 241 FVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL 300 FVVL RP G+EA+ +A+EL+ WV+ EIG IA+P++IRF DNLPKTRSGKIMRRLLR + Sbjct: 567 FVVLNDFRPEGEEARQLAEELKAWVSNEIGKIARPEDIRFADNLPKTRSGKIMRRLLRSI 626 Query: 301 AKGEDITQDISTLENPAILDQLKE 324 AK E ITQDISTLENP I+ QL++ Sbjct: 627 AKNEMITQDISTLENPQIIGQLQQ 650 >sll0542 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Syn Synechocystis Length = 653 Score = 389 bits (999), Expect = e-108, Method: Compositional matrix adjust. Identities = 200/329 (60%), Positives = 248/329 (75%), Gaps = 13/329 (3%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 ++EGVP N G FWD+++R+ IFYTAPTAIR+ I+ EA P DLSSLRLLG Sbjct: 334 MYEGVPRPSNPGCFWDVIERYGVNIFYTAPTAIRAFIRMGEAV----PNARDLSSLRLLG 389 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 +VGEPINPEAWMWY++ IG KCPIVDT+WQTETGG M++PLPGA P PGSCT P PGI Sbjct: 390 TVGEPINPEAWMWYHRVIGGGKCPIVDTWWQTETGGIMLTPLPGAIPTKPGSCTKPFPGI 449 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYF----PEEFGGKVYLA 176 A IVD GN + + GG LV+K+PWPSMIR ++ D +RF+ +Y+ P+E G +Y A Sbjct: 450 VAEIVDLDGNPVESDQGGFLVIKQPWPSMIRDVYGDTDRFRHTYWEHIQPKE-GQYLYFA 508 Query: 177 GDGAIRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGE 236 GDGA R+KD GYF + GR+DDV+NVSGHR+GTMEIESALV++P+VAEAAVVG+PDE TGE Sbjct: 509 GDGARRDKD-GYFWVMGRVDDVINVSGHRLGTMEIESALVSHPLVAEAAVVGRPDELTGE 567 Query: 237 SICAFVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRL 296 +I AFV L+ +E K K+L V +EIG IA+P EIRF D LPKTRSGKIMRRL Sbjct: 568 AIFAFVSLEGNAEPSEELK---KDLVKHVTEEIGAIARPAEIRFTDVLPKTRSGKIMRRL 624 Query: 297 LRVLAKGEDITQDISTLENPAILDQLKEA 325 LR LA G++I+ D STLE+ +LD+L+E Sbjct: 625 LRSLASGQEISGDTSTLEDRTVLDKLREG 653 >DR2471 [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Dra Deinococcus radiodurans Length = 649 Score = 372 bits (956), Expect = e-103, Method: Compositional matrix adjust. Identities = 187/325 (57%), Positives = 240/325 (73%), Gaps = 13/325 (4%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 ++EG P P+ GRFWD+VQ+H+ TI YTAPTAIRS ++ DE P +YDL+SLRLLG Sbjct: 335 MYEGAPNQPDWGRFWDIVQKHRVTILYTAPTAIRSFMQ--HGDEI--PGRYDLASLRLLG 390 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 SVGEPINPEAWMWYY+ IG E+CP+VDT+WQTETG M++ LPGA P PGS LP+ G+ Sbjct: 391 SVGEPINPEAWMWYYRVIGGERCPVVDTWWQTETGSIMLTTLPGAFPSKPGSAGLPMFGV 450 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPEEFGGKVYLAGDGA 180 A++ G ++ + GG+LV+KRPWPSM+RT++ D ER++KSY+ E VY AGDGA Sbjct: 451 EPALMTRDGEEIGDDDGGLLVIKRPWPSMLRTVYGDDERYRKSYWGEI--PHVYFAGDGA 508 Query: 181 IRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESICA 240 R+ D GY+TI GR+DDVLNVSGHR+GTMEIESALVA+P V+EAAVVG+PD GES+ A Sbjct: 509 RRDHD-GYYTIVGRVDDVLNVSGHRLGTMEIESALVAHPDVSEAAVVGRPDPVKGESVVA 567 Query: 241 FVVLKRPRPTGDEAKAIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVL 300 +V+L+ D A LR V+ EIG +A+P I D LPKTRSGKIMRR LR L Sbjct: 568 YVLLQ------DGHTADPAALRAHVSSEIGALARPDAIYIADALPKTRSGKIMRRFLRQL 621 Query: 301 AKGEDITQDISTLENPAILDQLKEA 325 A G+ + D STLE+P +L++L+ + Sbjct: 622 AAGQPVQGDTSTLEDPTVLERLQAS 646 >Cj1537c [I] COG0365 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases Cje Campylobacter jejuni Length = 657 Score = 360 bits (923), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 184/326 (56%), Positives = 235/326 (72%), Gaps = 10/326 (3%) Query: 1 VFEGVPTYPNAGRFWDMVQRHKATIFYTAPTAIRSLIKAAEADEKIHPKQYDLSSLRLLG 60 + EG PTYPN+GR+W M++ ++ + FYT+PTAIR L AD P++YDLS+L +LG Sbjct: 329 MHEGTPTYPNSGRWWRMIEEYQISKFYTSPTAIRML----HADAPNEPRKYDLSTLEVLG 384 Query: 61 SVGEPINPEAWMWYYKNIGQEKCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGI 120 +VGEPINP AW W+Y IG K PIVDT+WQTETGGHMI+PLPGATP PG TLPLPGI Sbjct: 385 TVGEPINPSAWKWFYDEIGGTKSPIVDTWWQTETGGHMITPLPGATPLKPGCATLPLPGI 444 Query: 121 TAAIVDETGNDLPNGTGGILVVKRPWPSMIRTIWNDPERFKKSYFPE--EFGGKVYLAGD 178 A ++DE GN G G+L + +PWPSMIR IW + ER+ +SYF + + G VY +GD Sbjct: 445 FAEVIDEEGNKKDEGEDGLLCITKPWPSMIRGIWGNDERYIESYFSQAKKDGKAVYFSGD 504 Query: 179 GAIRNKDTGYFTITGRIDDVLNVSGHRMGTMEIESALVANPIVAEAAVVGKPDETTGESI 238 GA +K+ GY TITGR DDV+NV+GHR+GT EIESA+ +P VAE+AVV D GES+ Sbjct: 505 GAFYDKN-GYITITGRTDDVVNVAGHRIGTAEIESAIAKHPSVAESAVVSILDTIKGESL 563 Query: 239 CAFVVLKRPRPTGDEAKAIA--KELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRL 296 AFVVL P + D AI KEL D + EIGPIAK ++I + LPKTRSGKIMRR+ Sbjct: 564 FAFVVLS-PASSCDLGGAIETLKELNDILRVEIGPIAKIEKILYTPGLPKTRSGKIMRRI 622 Query: 297 LRVLAKGEDITQDISTLENPAILDQL 322 LR +A+GE+I QDISTLE+ +++ + Sbjct: 623 LRTIARGEEIKQDISTLEDSKVVETI 648