BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0009.0084.p (381 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0766 [M] COG2230 Cyclopropane fatty acid synthase and related... 496 e-140 PA5546 [M] COG2230 Cyclopropane fatty acid synthase and related ... 376 e-104 DR2187 [M] COG2230 Cyclopropane fatty acid synthase and related ... 295 1e-79 mll4091 [M] COG2230 Cyclopropane fatty acid synthase and related... 279 6e-75 CAC0877 [M] COG2230 Cyclopropane fatty acid synthase and related... 266 6e-71 >RSc0766 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Rso Ralstonia solanacearum Length = 406 Score = 496 bits (1278), Expect = e-140, Method: Compositional matrix adjust. Identities = 246/378 (65%), Positives = 295/378 (78%), Gaps = 2/378 (0%) Query: 3 LELWNGRQFDFSD-NAPQVTLRVPHVSALPYLFRPSLSNLGEAYVEGKIDVDGPVASIIN 61 L LWNG Q+ + P VTL V SALP L P+L+NLGEAYV+ KID+DG + II+ Sbjct: 28 LRLWNGHQYPLGRFDRPTVTLTVREASALPLLLSPTLNNLGEAYVQEKIDLDGRLPDIIS 87 Query: 62 VANLLAAKTL-KAEGKLSRIVRTVRHSKEKDAEAIRYHYDVSNEFYKLFLDENMVYSCAY 120 V LAA L+R+VR H+KE D +IRYHYDVSN+FYKL+LD NMVYSCAY Sbjct: 88 VGYQLAASAAATGSNALARVVRHFAHTKEGDKASIRYHYDVSNDFYKLWLDRNMVYSCAY 147 Query: 121 FENGDEDLATAQIKKIDHILTKIKVRPGDRLLDIGCGWGALVLRAAQKFGAQCVGVTLSE 180 FE+GDE L AQIKKIDHILTKI+++P LLDIGCGWGALVLRAAQ+FGA+C+GVTLS+ Sbjct: 148 FEHGDETLDDAQIKKIDHILTKIRLQPDQTLLDIGCGWGALVLRAAQQFGARCLGVTLSQ 207 Query: 181 NQYALARERVEKAGLSDRIEIRLQDYRDVTGTFDRITSVGMFEHVGLKNLPLYFSHINAL 240 NQ+ LARERV AGL DRIEIRLQDYRD+TG FDRITSVGMFEHVG KNLP YF +++L Sbjct: 208 NQFDLARERVRAAGLEDRIEIRLQDYRDLTGAFDRITSVGMFEHVGRKNLPGYFRRVHSL 267 Query: 241 LADDGLVMNHGITSTDIENGESPYGGGEFVDKYVFPAGELPHVSLVLKTMQQGGLEVFDV 300 LAD+G+ MNHGITS+D E GES GGG+F+D+YVFP GELPH+SL L+ Q+GGLE DV Sbjct: 268 LADNGIAMNHGITSSDPEGGESSLGGGDFIDRYVFPQGELPHISLALRAAQEGGLEALDV 327 Query: 301 ENLRRHYARTCAIWADNFEARSEEIKKLTDEKRYRIWRVYLAGSSHGFDKDWISLYQVVC 360 E+LRRHYART WAD FE E I+K+ EK YRIWRVYLAG +H FD D +S++Q++C Sbjct: 328 ESLRRHYARTLEHWADRFETNGETIRKIVGEKTYRIWRVYLAGCAHAFDADEVSIFQILC 387 Query: 361 GKANRPAAALPQSRRYIY 378 K+ +PAA++P SRRYIY Sbjct: 388 QKSGKPAASIPWSRRYIY 405 >PA5546 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Pae Pseudomonas aeruginosa Length = 394 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 183/381 (48%), Positives = 259/381 (67%), Gaps = 6/381 (1%) Query: 1 LRLELWNGRQFDFSDNAPQVTLRVPHVSALPYLFRPSLSNLGEAYVEGKIDVDGPVASII 60 LR+ + + + FD + PQVT+ + S L + PSL LG+AYVE ++D++GP++ +I Sbjct: 16 LRIRIGDAQAFDLGPD-PQVTMVIRDPSLLTEITHPSLDALGKAYVEKRLDIEGPISQVI 74 Query: 61 NVANLLAAKTLKAEGKLSRIVRTVRHSKEKDAEAIRYHYDVSNEFYKLFLDENMVYSCAY 120 + + L+ + +G + + H K+ DAEAI YHYD+SN+FY+L+LD MVYSCAY Sbjct: 75 GIGDALSEALGEPDGGAPPVRES--HDKDTDAEAIHYHYDLSNDFYQLWLDPEMVYSCAY 132 Query: 121 FENGDEDLATAQIKKIDHILTKIKVRPGDRLLDIGCGWGALVLRAAQKFGAQCVGVTLSE 180 FE G EDLATAQ+ K+ H+ K++++PG++LLD+GCGWG L AA++FG + G+TLS+ Sbjct: 133 FETGKEDLATAQLAKLRHLCRKLRLQPGEKLLDVGCGWGGLARLAAREFGVEVTGITLSK 192 Query: 181 NQYALARERVEKAGLSDRIEIRLQDYRDVT--GTFDRITSVGMFEHVGLKNLPLYFSHIN 238 Q AL RERV+ GL DR+ ++L DYRD+ G FD++ SVGMFEHVG NL LYF + Sbjct: 193 EQLALGRERVKAEGLEDRVTLKLLDYRDLPRDGRFDKVVSVGMFEHVGHANLGLYFQQLY 252 Query: 239 ALLADDGLVMNHGITSTDIENGESPYGGGEFVDKYVFPAGELPHVSLVLKTMQQGGLEVF 298 + GLVMNHGITS + G G+F+D+YVFP GELPH+SL + M + GLEV Sbjct: 253 EAVRPGGLVMNHGITSRYTDGRPVGKGAGDFIDRYVFPHGELPHLSLAVARMSEAGLEVV 312 Query: 299 DVENLRRHYARTCAIWADNFEARSEEIKKLTDEKRYRIWRVYLAGSSHGFDKDWISLYQV 358 DVE+LR HYART W+ N EAR +E +L E+ RIWR+YLAG ++GF + WI+L+Q+ Sbjct: 313 DVESLRLHYARTLDFWSANLEARLKEAARLVPEETLRIWRLYLAGCAYGFKRGWINLHQI 372 Query: 359 VCGKANRPAA-ALPQSRRYIY 378 + + + + LP SRR +Y Sbjct: 373 LAIRPHEDGSHELPWSRRDLY 393 >DR2187 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Dra Deinococcus radiodurans Length = 462 Score = 295 bits (756), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 175/403 (43%), Positives = 227/403 (56%), Gaps = 28/403 (6%) Query: 3 LELWNGRQFDFSDNAPQVTLRVPHVSALPYLFR-PSLSNLGEAYVEGKIDVDGPVASIIN 61 LELW+G Q L + AL + R P LGEAY+ G D++G ++ Sbjct: 60 LELWDGTVLPAGRTPAQARLTLKTEHALGRMLRLPVDLALGEAYLRGDFDIEGDFGAVAG 119 Query: 62 VAN-------------LLAAKTL--KAEGKLSRIVRTV----RHSKEKDAEAIRYHYDVS 102 +A+ LLA L + G++ R V +HS+E+D +AI+YHYDVS Sbjct: 120 LADDFGELPTLAQVPGLLADVDLLRRGAGEMPRPVSVSLEGEQHSRERDQQAIQYHYDVS 179 Query: 103 NEFYKLFLDENMVYSCAYFENGDEDLATAQIKKIDHILTKIKVRPGDRLLDIGCGWGALV 162 N+FYKL+LDE MVYSCAYF G E L AQ K+D+I K+++RPG+RLLDIGCGWG L Sbjct: 180 NDFYKLWLDERMVYSCAYFPGGQETLDEAQTAKLDYICRKLQLRPGERLLDIGCGWGGLS 239 Query: 163 LRAAQKFGAQCVGVTLSENQYALARERVEKAGLSDRIEIRLQDYRDV----TGTFDRITS 218 L AAQ +G Q +GVTLS+ Q + RV AGL ++++ L+DYRDV FD+I S Sbjct: 240 LYAAQHYGVQVLGVTLSQAQLQEGQARVRVAGLEGQVQLELRDYRDVLSRGPAQFDKIAS 299 Query: 219 VGMFEHVGLKNLPLYFSHINALLADDGLVMNHGITSTDIENGESPY--GGGEFVDKYVFP 276 VGM EHVG +N+P YF A L GL++NH I I P G F KYVFP Sbjct: 300 VGMAEHVGRRNMPEYFRSAYAALKPGGLMLNHAI-GDGIGQARVPMWLQSGNFARKYVFP 358 Query: 277 AGELPHVSLVLKTMQQGGLEVFDVENLRRHYARTCAIWADNFEARSEEIKKLTDEKRYRI 336 GEL V LK + EV DVENLR HYA T WA EA E L E+R R+ Sbjct: 359 DGELLPVWETLKYASEQLFEVRDVENLREHYALTIGHWAARLEAHRPEALALLGEERLRL 418 Query: 337 WRVYLAGSSHGFDKDWISLYQVVCGKAN-RPAAALPQSRRYIY 378 WR+YL +S+ F K ++L+Q + K + A LP SR +Y Sbjct: 419 WRLYLGATSYYFRKGHLTLFQSLLAKPDAERAVPLPLSRAGLY 461 >mll4091 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Mlo Mesorhizobium loti Length = 422 Score = 279 bits (714), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 158/405 (39%), Positives = 232/405 (57%), Gaps = 36/405 (8%) Query: 3 LELWNGRQFDFSDNAPQVTLRVPHVSALPYLFRPSLSNLGEAYVEGKIDV-DG------- 54 ++LW G + P +T+ P + + RP+ S L E ++ +DV DG Sbjct: 24 VKLWTGERIG-PTTGPLLTINDPDI-VWQMVRRPTFSTLIEMWISKAVDVEDGSLFDFYA 81 Query: 55 --------------PVASIIN--VANLLAAKTLKAEGKLSRIVRTVRHSKEKDAEAIRYH 98 P +I+ A L + K + A LS V S + EAI++H Sbjct: 82 LPSQGKLRSKLKTLPKLAILRDLPAVLFSRKQMTARTDLSGRNPFVSGSSQ---EAIQHH 138 Query: 99 YDVSNEFYKLFLDENMVYSCAYFENGDEDLATAQIKKIDHILTKIKVRPGDRLLDIGCGW 158 YD+SN FY+LFLDE MVYSC YF++ + AQ K+DHI K++++PG+RLLDIGCGW Sbjct: 139 YDISNAFYRLFLDERMVYSCGYFKDFANGIDEAQADKLDHICRKLRLKPGERLLDIGCGW 198 Query: 159 GALVLRAAQKFGAQCVGVTLSENQYALARERVEKAGLSDRIEIRLQDYRDVTGTFDRITS 218 GA+++ AA+ +G GV+LSE Q LARER+ GL D+I I ++ Y ++TGTFD+I+S Sbjct: 199 GAMLIHAAKNYGVTGHGVSLSEAQTKLARERIRAEGLEDKITIEIKSYAELTGTFDKISS 258 Query: 219 VGMFEHVGLKNLPLYFSHINALLADDGLVMNHGIT---STDIENGESPYGGGEF--VDKY 273 +GMFEH+GL N YFS ++ LL G+ ++H IT DI+ ++ G E+ + KY Sbjct: 259 IGMFEHLGLANHAAYFSTVHRLLKPGGIYLHHAITRRSKGDIK--KTLRKGPEYKALIKY 316 Query: 274 VFPAGELPHVSLVLKTMQQGGLEVFDVENLRRHYARTCAIWADNFEARSEEIKKLTDEKR 333 +FP GEL + + L ++ G V DVENLR HYARTC +WA+ +AR +E E + Sbjct: 317 IFPGGELDTIGMTLGNLEAHGFLVADVENLREHYARTCRLWAERLQARFDEAIAEVGEAK 376 Query: 334 YRIWRVYLAGSSHGFDKDWISLYQVVCGKANRPAAALPQSRRYIY 378 R+W +YL G S FD+ ++Q V + R + LP +R +Y Sbjct: 377 ARLWLLYLTGCSITFDRGSAQIFQTVVTRRARGPSGLPPTRADLY 421 >CAC0877 [M] COG2230 Cyclopropane fatty acid synthase and related methyltransferases Cac Clostridium acetobutylicum Length = 391 Score = 266 bits (680), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 130/344 (37%), Positives = 211/344 (61%), Gaps = 10/344 (2%) Query: 36 PSLSNLGEAYVEGKIDVDGPVASIINVANLLAAKTLKAEGKLSRIVRTVRHSKEKDAEAI 95 PS++ GE Y+E K+D++G V +I ++ K S++++ + ++ K + I Sbjct: 56 PSMA-FGEGYMEKKLDIEGSVEDVIQSLYNNKESFMRNGEKYSKLIKILPNTIRKSKKDI 114 Query: 96 RYHYDVSNEFYKLFLDENMVYSCAYFENGDEDLATAQIKKIDHILTKIKVRPGDRLLDIG 155 ++HYD+ N+FYKL+LD+ M YSC YF++ ++ L AQ K++HIL K+ ++ LLDIG Sbjct: 115 KFHYDIGNDFYKLWLDKTMTYSCGYFKSPNDSLEQAQKNKVEHILKKLSLKKDVTLLDIG 174 Query: 156 CGWGALVLRAAQKFGAQCVGVTLSENQYALARERVEKAGLSDRIEIRLQDYRDVTG-TFD 214 CGWG L++ AA+++G + +GVTLSE Q+ + +ERV+K GL R+++ L+DYR++ TFD Sbjct: 175 CGWGELIITAAKEYGVKALGVTLSEEQFKVVKERVKKEGLEGRVDVILEDYREIKNRTFD 234 Query: 215 RITSVGMFEHVGLKNLPLYFSHINALLADDGLVMNHGITSTDIENGESPYGGGEFVDKYV 274 R+ SVGM EHVG +N+ YF +N LL G+ + H IT G G +++ Y+ Sbjct: 235 RVVSVGMIEHVGKENIAEYFETVNKLLNKGGISLLHCIT------GVKKGGTNTWINNYI 288 Query: 275 FPAGELPHVSLVLKTMQQGGLEVFDVENLRRHYARTCAIWADNFEARSEEIKKLTDEKRY 334 FP G +P V ++ + + + D E+LRRHY +T WA+NFE +EIKK+ +E Sbjct: 289 FPGGYVPAVRELVNNISEQDFYLVDAESLRRHYGKTLECWAENFENAMDEIKKMKEEPFI 348 Query: 335 RIWRVYLAGSSHGFDKDWISLYQVVCGKANRPAAALPQSRRYIY 378 R+WR+YL + F+ I ++Q + K + +R Y+Y Sbjct: 349 RMWRLYLNACAASFNSGNIDIHQFLFTKD--INDEISWTREYMY 390