BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0020.p (448 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0691 [Q] COG3508 Homogentisate 1,2-dioxygenase Rso Ralstonia ... 688 0.0 PA2009 [Q] COG3508 Homogentisate 1,2-dioxygenase Pae Pseudomonas... 559 e-159 CC2532 Non COG 514 e-145 mlr8303 [Q] COG3508 Homogentisate 1,2-dioxygenase Mlo Mesorhizob... 512 e-145 SMc03208 [Q] COG3508 Homogentisate 1,2-dioxygenase Sme Sinorhizo... 474 e-133 >RSp0691 [Q] COG3508 Homogentisate 1,2-dioxygenase Rso Ralstonia solanacearum Length = 448 Score = 688 bits (1775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/444 (74%), Positives = 368/444 (82%), Gaps = 7/444 (1%) Query: 5 AESFIAKAAINTVGYQSGFGNEFATEALPGALPANQNSPQQVAYGLYAEQLSGTAFTAPR 64 A++ A+ + YQSGFGNEFATEALPGALP QNSPQQ YGLYAEQLSGTAFTAPR Sbjct: 8 AKNAFTPASPDRPAYQSGFGNEFATEALPGALPHGQNSPQQAPYGLYAEQLSGTAFTAPR 67 Query: 65 GHNRRSWLYRIRPAAMHAPFERMSNGLIDRFDD----AETPPNQYRWDPHAMPDAPTDFV 120 HNRR+WLYRIRPAA+H PFE ++ DRF PNQ RWDP P A TDF+ Sbjct: 68 AHNRRAWLYRIRPAAVHLPFEPIAQ---DRFHSDFHAVPASPNQLRWDPLPAPAAGTDFI 124 Query: 121 AGLITMAGNGSPDAQTGCAIHLYAANQSMTDSYFYSADGELLIVPQAGRLQLLTELGAID 180 G++T AGNG PDAQTGC IHLYAAN SMT +FY+ADGELLIVPQ GRL+LLTELG +D Sbjct: 125 DGIVTFAGNGGPDAQTGCGIHLYAANASMTGRFFYNADGELLIVPQQGRLRLLTELGVLD 184 Query: 181 IEPQEIAVIPRGVRFQVKLPDGSAQGYVCENFGALLRLPDLGPLGSNGLANPRDFLTPHA 240 +EP EIAVIPRGVRF+V+LPDG A+GY+CENFGA+ RLPDLG +GSNGLANPRDFLTPHA Sbjct: 185 VEPLEIAVIPRGVRFRVELPDGEARGYLCENFGAIFRLPDLGVIGSNGLANPRDFLTPHA 244 Query: 241 WYEDREGDFRLIAKFGGNLWQAKIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISYDHPD 300 WYEDREGDF L+AKF GNLW+A+IGHSPLDVVAWHGNYAPYKYDLR FNTIGSISYDHPD Sbjct: 245 WYEDREGDFELVAKFHGNLWRARIGHSPLDVVAWHGNYAPYKYDLRLFNTIGSISYDHPD 304 Query: 301 PSIFLVLQAQSDTPGVDTLDFVIFPPRWLAAENTFRPPWFHRNVASEFMGLIHGVYDAKA 360 PSIFLVLQ+ SDTPGVD +DFVIFPPRWLA E++FRPPWFHRN+ASEFMGLI GVYDAKA Sbjct: 305 PSIFLVLQSVSDTPGVDAIDFVIFPPRWLAMEHSFRPPWFHRNIASEFMGLIQGVYDAKA 364 Query: 361 EGFRPGGASLHNCMTGHGPDGATFEKAIRSDTSKPDKVGDTMAFMFETRNIIKPTRHALS 420 EGF PGGASLHNCMTGHGPD TFEKA +DT++P KV TMAFMFETR +I+PTR A Sbjct: 365 EGFVPGGASLHNCMTGHGPDAETFEKASHADTTQPHKVEATMAFMFETRGVIRPTRFAAE 424 Query: 421 SPTLQKNYYQCWMDINKHFNPQHR 444 S LQ Y++CW + KHF+P R Sbjct: 425 SAQLQARYFECWQGLKKHFDPAKR 448 >PA2009 [Q] COG3508 Homogentisate 1,2-dioxygenase Pae Pseudomonas aeruginosa Length = 432 Score = 559 bits (1441), Expect = e-159, Method: Compositional matrix adjust. Identities = 266/430 (61%), Positives = 323/430 (75%), Gaps = 3/430 (0%) Query: 15 NTVGYQSGFGNEFATEALPGALPANQNSPQQVAYGLYAEQLSGTAFTAPRGHNRRSWLYR 74 + YQSGFGNEF++EALPGALP QNSPQ+ YGLYAE LSGTAFT R RR+WLYR Sbjct: 6 TALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYR 65 Query: 75 IRPAAMHAPFERMSNGLIDRFDDAETPPNQYRWDPHAMPDAPTDFVAGLITMAGNGSPDA 134 I P+A H PF R+ + DA TP N+ RWDP A+P+ PTDF+ GL+ MA N D Sbjct: 66 ITPSAKHPPFRRLERQIAGAELDAPTP-NRLRWDPLALPEQPTDFLDGLLRMAANAPGDK 124 Query: 135 QTGCAIHLYAANQSMTDSYFYSADGELLIVPQAGRLQLLTELGAIDIEPQEIAVIPRGVR 194 G +I+ Y AN+SM + FY ADGELL+VPQ GRL+L TELGA+ +EP EIAVIPRG++ Sbjct: 125 PAGVSIYQYLANRSM-ERCFYDADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMK 183 Query: 195 FQVKLPDGSAQGYVCENFGALLRLPDLGPLGSNGLANPRDFLTPHAWYEDREGDFRLIAK 254 F+V+L DG A+GY+ EN GA LRLPDLGP+GSNGLANPRDFLTP A YED +L+ K Sbjct: 184 FRVELLDGEARGYIAENHGAPLRLPDLGPIGSNGLANPRDFLTPVARYEDSRQPLQLVQK 243 Query: 255 FGGNLWQAKIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISYDHPDPSIFLVLQAQSDTP 314 + G LW ++ HSPLDVVAWHGN PYKYDLRRFNTIG++S+DHPDPSIF VL + + P Sbjct: 244 YLGELWACELDHSPLDVVAWHGNNVPYKYDLRRFNTIGTVSFDHPDPSIFTVLTSPTSVP 303 Query: 315 GVDTLDFVIFPPRWLAAENTFRPPWFHRNVASEFMGLIHGVYDAKAEGFRPGGASLHNCM 374 G+ +DFVIFPPRW+ AENTFRPPWFHRN+ +EFMGLI G YDAKA GF PGGASLH+CM Sbjct: 304 GLANIDFVIFPPRWMVAENTFRPPWFHRNLMNEFMGLIQGAYDAKAGGFVPGGASLHSCM 363 Query: 375 TGHGPDGATFEKAIRSDTSKPDKVGDTMAFMFETRNIIKPTRHALSSPTLQKNYYQCWMD 434 + HGPD + +KAI +D KP ++ TMAFMFET +++P+R AL +P LQ +Y CW Sbjct: 364 SAHGPDAESCDKAIAADL-KPHRIDQTMAFMFETSQVLRPSRAALETPALQNDYDACWAS 422 Query: 435 INKHFNPQHR 444 + FNPQ R Sbjct: 423 LVSTFNPQRR 432 >CC2532 Non COG Length = 425 Score = 514 bits (1325), Expect = e-145, Method: Compositional matrix adjust. Identities = 253/420 (60%), Positives = 306/420 (72%), Gaps = 4/420 (0%) Query: 19 YQSGFGNEFATEALPGALPANQNSPQQVAYGLYAEQLSGTAFTAPRGHNRRSWLYRIRPA 78 YQ+GFG+ F TEA PGALP +NSPQ+V +GLYAEQLSG+AFTAPR NRR+WLYR+RP+ Sbjct: 5 YQTGFGSYFETEAHPGALPVGRNSPQKVPFGLYAEQLSGSAFTAPRHENRRTWLYRLRPS 64 Query: 79 AMHAPFERMS-NGLIDRFDDAETPPNQYRWDPHAMPDAPTDFVAGLITMAGNGSPDAQTG 137 A H ++ + + L+ F TP N+ RW P +P APTDFV GL+++AGN G Sbjct: 65 AGHEAYQPYAQDKLVSAFPGPATP-NRLRWSPLEIPAAPTDFVDGLVSLAGNADAATLGG 123 Query: 138 CAIHLYAANQSMTDSYFYSADGELLIVPQAGRLQLLTELGAIDIEPQEIAVIPRGVRFQV 197 A H+Y N SM + FY+ADGE+LIVPQ G L L+TE+G + P IAVIPRGVRF+V Sbjct: 124 IAAHVYLVNVSMKNRVFYNADGEMLIVPQMGELTLVTEMGVLKAGPGHIAVIPRGVRFRV 183 Query: 198 KLPDGSAQGYVCENFGALLRLPDLGPLGSNGLANPRDFLTPHAWYEDREGDFRLIAKFGG 257 ++ +G A+GYVCEN+GA RLP+LGP+G+NGLANPRDF P A +ED + ++I KF G Sbjct: 184 EV-EGPARGYVCENYGAAFRLPELGPIGANGLANPRDFEAPVAAFEDIDTPTQVIQKFQG 242 Query: 258 NLWQAKIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISYDHPDPSIFLVLQAQSDTPGVD 317 LW A HSPLDVVAWHGN PY+YDL RFNTI ++SYDHPDPSIF VL + SDTPG Sbjct: 243 ALWAATWDHSPLDVVAWHGNLTPYRYDLSRFNTINTVSYDHPDPSIFTVLTSPSDTPGTA 302 Query: 318 TLDFVIFPPRWLAAENTFRPPWFHRNVASEFMGLIHGVYDAKAEGFRPGGASLHNCMTGH 377 DFVIFPPRW+ AE+TFRPPWFHRNV SEFMGLIHG YDAKA GF PGGASLHNCM+ H Sbjct: 303 NCDFVIFPPRWMVAEDTFRPPWFHRNVMSEFMGLIHGAYDAKAGGFVPGGASLHNCMSDH 362 Query: 378 GPDGATFEKAIRSDTSKPDKVGDTMAFMFETRNIIKPTRHALSSPTLQKNYYQCWMDINK 437 GPD A+ +KA P K+ TMAFMFE+R + +PT AL S LQ +Y CW K Sbjct: 363 GPDVASHKKATEV-VLAPHKIDGTMAFMFESRWVFRPTHLALESAALQSDYDACWTGFPK 421 >mlr8303 [Q] COG3508 Homogentisate 1,2-dioxygenase Mlo Mesorhizobium loti Length = 457 Score = 512 bits (1319), Expect = e-145, Method: Compositional matrix adjust. Identities = 253/426 (59%), Positives = 302/426 (70%), Gaps = 5/426 (1%) Query: 19 YQSGFGNEFATEALPGALPANQNSPQQVAYGLYAEQLSGTAFTAPRGHNRRSWLYRIRPA 78 Y GFGN+F TE LPG+LP +NSPQ+ AYGLYAEQLSG+ FTAPRG N RSWLYRIRP+ Sbjct: 25 YMPGFGNDFETETLPGSLPQGRNSPQRPAYGLYAEQLSGSPFTAPRGTNERSWLYRIRPS 84 Query: 79 AMH-APFERMSNGLID---RFDDAETPPNQYRWDPHAMPDAPTDFVAGLITMAGNGSPDA 134 H F+ S L D E QYRW+P MP PTDF+ G+ ++ G Sbjct: 85 VKHTGRFKAASYPLWKTAPNVGDHELALGQYRWNPVPMPKEPTDFIQGMRSITTAGDVLG 144 Query: 135 QTGCAIHLYAANQSMTDSYFYSADGELLIVPQAGRLQLLTELGAIDIEPQEIAVIPRGVR 194 QTG A H+Y AN+SM D +F++ADGELL+VPQ G L+ +TE+G I++ P EIAV+PRG+ Sbjct: 145 QTGMAAHVYVANRSMVDDHFFNADGELLVVPQVGALRFVTEMGVIELRPGEIAVLPRGLV 204 Query: 195 FQVKLPDGSAQGYVCENFGALLRLPDLGPLGSNGLANPRDFLTPHAWYEDREGDFRLIAK 254 F+ +L D +GYVCEN+GA L LPD GP+G+N LANPRDF TP AW+E++E RLI K Sbjct: 205 FKAELVDKDVRGYVCENYGAKLTLPDRGPIGANCLANPRDFKTPCAWFEEKETPCRLIVK 264 Query: 255 FGGNLWQAKIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISYDHPDPSIFLVLQAQSDTP 314 + GN +IGHSPLDVVAWHGNYAPYKYDL F+ +G+I +DHPDPSIF VL A S Sbjct: 265 WCGNFHVTEIGHSPLDVVAWHGNYAPYKYDLATFSPVGAILFDHPDPSIFTVLTAPSGEE 324 Query: 315 GVDTLDFVIFPPRWLAAENTFRPPWFHRNVASEFMGLIHGVYDAKAEGFRPGGASLHNCM 374 G +DFVIFPPRWL AE+TFRPPW+HRN+ SEFMGLIHG YDAK EGF PGG SLHN M Sbjct: 325 GTANIDFVIFPPRWLVAEDTFRPPWYHRNIMSEFMGLIHGQYDAKEEGFVPGGISLHNLM 384 Query: 375 TGHGPDGATFEKAIRSDTSKPDKVGDTMAFMFETRNIIKPTRHALSSPTLQKNYYQCWMD 434 HGPD FEKA R+D KP K+ +TMAFMFETR TR+ T Q NY CW D Sbjct: 385 LAHGPDAPGFEKASRADL-KPVKLDNTMAFMFETRFPQMLTRYGAELETRQDNYIDCWAD 443 Query: 435 INKHFN 440 + K FN Sbjct: 444 LKKRFN 449 >SMc03208 [Q] COG3508 Homogentisate 1,2-dioxygenase Sme Sinorhizobium meliloti Length = 453 Score = 474 bits (1219), Expect = e-133, Method: Compositional matrix adjust. Identities = 243/436 (55%), Positives = 293/436 (67%), Gaps = 21/436 (4%) Query: 18 GYQSGFGNEFATEALPGALPANQNSPQQVAYGLYAEQLSGTAFTAPRGHNRRSWLYRIRP 77 GY GFGN+F TE+LPGALP QNSPQ+ YGLYAEQLSG+ FTAPRG N RSWLYRIRP Sbjct: 20 GYMPGFGNDFETESLPGALPQGQNSPQKCNYGLYAEQLSGSPFTAPRGTNERSWLYRIRP 79 Query: 78 AAMHAPFERMSNGLIDRFD--DAETPPN---------QYRWDPHAMPDAPTDFVAGLITM 126 + H G R D +T P+ Q RW P P DF+ G+ TM Sbjct: 80 SVRH-------TGRFRRVDYPHWKTAPHVGEHSLALGQLRWSPLPAPSEALDFLQGIRTM 132 Query: 127 AGNGSPDAQTGCAIHLYAANQSMTDSYFYSADGELLIVPQAGRLQLLTELGAIDIEPQEI 186 G Q G A H YA N M D YF++ADGELLIVP+ G +Q+ TELG +D+EP EI Sbjct: 133 TTAGDALTQAGMAAHAYAFNADMVDDYFFNADGELLIVPETGAIQVFTELGRMDVEPSEI 192 Query: 187 AVIPRGVRFQV-KLPDGSA-QGYVCENFGALLRLPDLGPLGSNGLANPRDFLTPHAWYED 244 +IPRG+ F+V +L + +GY+CEN+GA LPD GP+G+N LANPRDF TP A YED Sbjct: 193 CLIPRGMMFKVTRLGEEKVWRGYICENYGAKFTLPDRGPIGANCLANPRDFKTPVAAYED 252 Query: 245 REGDFRLIAKFGGNLWQAKIGHSPLDVVAWHGNYAPYKYDLRRFNTIGSISYDHPDPSIF 304 +E R+ K+ G+ +IGHSPLDVVAWHGNYAPYKYDL+ F+ +G+I +DHPDPSIF Sbjct: 253 KETPCRVQVKWCGSFHMVEIGHSPLDVVAWHGNYAPYKYDLKTFSPVGAILFDHPDPSIF 312 Query: 305 LVLQAQSDTPGVDTLDFVIFPPRWLAAENTFRPPWFHRNVASEFMGLIHGVYDAKAEGFR 364 VL A S G +DFVIFPPRWL AE+TFRPPW+HRN+ SEFMGLI+G YDAK EGF Sbjct: 313 TVLTAPSGEEGTANVDFVIFPPRWLVAEHTFRPPWYHRNIMSEFMGLIYGRYDAKEEGFV 372 Query: 365 PGGASLHNCMTGHGPDGATFEKAIRSDTSKPDKVGDTMAFMFETRNIIKPTRHALSSPTL 424 PGG SLHN M HGPD + FEKA + KP K+ +TMAFMFETR + T A TL Sbjct: 373 PGGMSLHNMMLAHGPDFSGFEKASNGEL-KPVKLDNTMAFMFETRFPQQLTTFAAELDTL 431 Query: 425 QKNYYQCWMDINKHFN 440 Q +Y CW + + F+ Sbjct: 432 QDDYMDCWSGLERKFD 447