BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0184.p (667 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA1721 [L] COG0210 Superfamily I DNA and RNA helicases Sau Staph... 83 2e-15 BU598 [L] COG0210 Superfamily I DNA and RNA helicases Buc Buchne... 82 4e-15 BS_yerF [L] COG0210 Superfamily I DNA and RNA helicases Bsu Baci... 77 1e-13 jhp1371 [L] COG0210 Superfamily I DNA and RNA helicases jHp Heli... 75 4e-13 HP1478 [L] COG0210 Superfamily I DNA and RNA helicases Hpy Helic... 72 4e-12 >SA1721 [L] COG0210 Superfamily I DNA and RNA helicases Sau Staphylococcus aureus N315 Length = 730 Score = 82.8 bits (203), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 112/458 (24%), Positives = 197/458 (43%), Gaps = 74/458 (16%) Query: 215 DLVPDLVELLSNHPSALEILPIKGLYL-IDEWHDINAAEFRLLQLLLRGNK-LVAVGDRY 272 DL+ + L P LE K Y+ +DE+ D N A++ L++LL K L VGD Sbjct: 190 DLIMTTINLFERVPEVLEYYQNKFQYIHVDEYQDTNKAQYTLVKLLASKFKNLCVVGDSD 249 Query: 273 QIINSTRGADIVYSTAGFEMAFPNAQYLPLGKSYRFGASLSKLAQRL----TARKCASHE 328 Q I RGADI + FE +P A + L ++YR ++ A + + RK Sbjct: 250 QSIYGWRGADI-QNILSFEKDYPEANTIFLEQNYRSTKTILNAANEVIKNNSERKPKGLW 308 Query: 329 DLYTEIKRLTYDPTLSGNCAAKVTQQVQALVK-ARADTRYSDIAIIVREPDQTIEIENLL 387 T +++ Y ++ A+ ++ ++K R +Y D+AI+ R Q+ +E Sbjct: 309 TANTNGEKIHYYEAMTERDEAEFV--IREIMKHQRNGKKYQDMAILYRTNAQSRVLEETF 366 Query: 388 LDQNIPYYCDDIESYLHRPEILMLRGLLHMASNTFATLQGDKESIKRMVMGFSLYLP--- 444 + N+PY + + R EI L L + +N+ D S++R++ +P Sbjct: 367 MKSNMPYTMVGGQKFYDRKEIKDLLSYLRIIANS-----NDDISLQRII-----NVPKRG 416 Query: 445 VGESSEDWERGY-----------FAEGSWFEEAQRMIAEQPNALKDFFEAVLCKPRDVDD 493 VG SS + + Y E + ++++ E N F+E + Sbjct: 417 VGPSSVEKVQNYALQNNISMFDALGEADFIGLSKKVTQECLN----FYELI--------- 463 Query: 494 DMTLRWKQRFTEVVEEMQKLNQESSAAELLNFANRHLSLVDATSRAFINRSRATSAAKSI 553 +L +Q F E+ E + ++ Q+S E+L N +RSR + + + Sbjct: 464 -QSLIKEQEFLEIHEIVDEVLQKSGYREMLEREN-----------TLESRSRLENIDEFM 511 Query: 554 QTFIAFATRHP--EMNAADFLAELHRRQQKVSKNRNAIQSRRQLTLTTIQAAKGRQWPHV 611 + P E + +FL +L + + + + +TL T+ +AKG ++P V Sbjct: 512 SVPKDYEENTPLEEQSLINFLTDL-----SLVADIDEADTENGVTLMTMHSAKGLEFPIV 566 Query: 612 LVPYLQQHEFPRLRDIA--------EEVRHFYVAMTRA 641 + +++ FP +R I EE R YVA+TRA Sbjct: 567 FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRA 604 >BU598 [L] COG0210 Superfamily I DNA and RNA helicases Buc Buchnera sp. APS Length = 645 Score = 82.0 bits (201), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 108/436 (24%), Positives = 187/436 (42%), Gaps = 71/436 (16%) Query: 241 LIDEWHDINAAEFRLLQLLLRGNK-LVAVGDRYQIINSTRGADIVYSTAGFEMAFPNAQY 299 L+DE+ D N +++ L+++L N VGD Q I S RGA+ + + FPN + Sbjct: 212 LVDEYQDTNNSQYELIKMLANKNSDFTLVGDDDQSIYSWRGAN-PQNIFLLKKDFPNLKI 270 Query: 300 LPLGKSYRFGASLSKLAQRLTARKCASHED-LYTEIKRLTYDPTLSG----NCAAKVTQQ 354 + + +YR + K A L A E L++++K L G N A K+ ++ Sbjct: 271 IKMEHNYRSSGRILKAANSLIANNIHYLEKKLFSQLKYGNLIKVLIGKNEENEAQKIVKK 330 Query: 355 VQALVKARADTRYSDIAIIVREPDQTIEIENLLLDQNIPYYCDDIESYLHRPEILMLRGL 414 + + A+ +Y D AI+ R Q+ +E L+ +NIPY + S+ RPEI L Sbjct: 331 IISQY-AKKKIKYRDFAILYRGNYQSRILEKALIKENIPYNISEKSSFFSRPEIKDLLSY 389 Query: 415 LHMASNTFATLQGDKESIKRMVMGFSLYLP---VGESS----EDWER----GYFAEGSWF 463 L + N + D + R+V +P +G+++ E+W F + Sbjct: 390 LRVVIN-----RDDNHAFMRIVN-----IPSRQIGKTTLKKLEEWANKKHVSLFQASNNI 439 Query: 464 EEAQRMIAEQPNALKDF------FEAVLC-KPRDVDDDMT--LRWKQRFTEVVEEMQKLN 514 E + + +K+F F A C KP ++ DD+ L +++ ++ +++ K+ Sbjct: 440 EIKKFLNENTIKKIKNFISKIEKFTAWSCLKPSNIIDDIVDDLEYEKWLSKFLKDPNKIK 499 Query: 515 QESSAAELLNFANRHLSLVDATSRAFINRSRATSAAKSIQTFIAFATRHPEMNAADFLAE 574 ++ V S+ F N + K + T TR M D L + Sbjct: 500 NS-------------INNVHTLSQWFKNMIKGDDFEKPM-TLFQIVTR---MTIRDILDD 542 Query: 575 LHRRQQKVSKNRNAIQSRRQLTLTTIQAAKGRQWPHVLVPYLQQHEFPRLR-----DIAE 629 ++Q Q R Q L T+ A+KG ++P V + + + P + +I E Sbjct: 543 NIIKEQ---------QDRVQ--LMTLHASKGLEFPAVFIIGMCEGILPNQKSIDNDNIEE 591 Query: 630 EVRHFYVAMTRAISSL 645 E R YV +TRA L Sbjct: 592 ERRLTYVGITRAKKQL 607 >BS_yerF [L] COG0210 Superfamily I DNA and RNA helicases Bsu Bacillus subtilis Length = 739 Score = 77.4 bits (189), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 128/461 (27%), Positives = 199/461 (43%), Gaps = 64/461 (13%) Query: 215 DLVPDLVELLSNHPSALEILPIKGLYL-IDEWHDINAAEFRLL-QLLLRGNKLVAVGDRY 272 DL+ ++L P LE K Y+ +DE+ D N A++ L+ QL R L VGD Sbjct: 194 DLIMTTIKLFDRVPEVLEFYQRKFQYIHVDEYQDTNRAQYMLVKQLAERFQNLCVVGDSD 253 Query: 273 QIINSTRGADIVYSTAGFEMAFPNAQYLPLGKSYRFGASLSKLAQRLTARKCASH-EDLY 331 Q I RGADI + FE +PNA + L ++YR + + A + ++L+ Sbjct: 254 QSIYRWRGADIT-NILSFEKDYPNASVILLEQNYRSTKRILRAANEVIKNNSNRKPKNLW 312 Query: 332 TE----IKRLTY---DPTLSGNCAAKVTQQVQALVKARADTRYSDIAIIVREPDQTIEIE 384 TE IK Y + G A Q+ + K + SDIAI+ R Q+ IE Sbjct: 313 TENDEGIKISYYRGDNEFGEGQFVAGKIHQLHSTGK----RKLSDIAILYRTNAQSRVIE 368 Query: 385 NLLLDQNIPYYCDDIESYLHRPEILMLRGLLHMASNTFATLQGDKESIKRMVMGFSLYLP 444 LL + Y + R EI + L + SN D S R+V +P Sbjct: 369 ETLLKAGLNYNIVGGTKFYDRKEIKDILAYLRLVSNP-----DDDISFTRIV-----NVP 418 Query: 445 ---VGESSEDWERGYFAEG--SWFEEAQRM-----IAEQPNALKDFFEAVLCKPRDVDDD 494 VG +S + Y A S+F+ Q++ A+ NAL D F ++ ++ D Sbjct: 419 KRGVGATSLEKIASYAAINGLSFFQAIQQVDFIGVSAKAANAL-DSFRQMIENLTNMQDY 477 Query: 495 MTLRWKQRFTEVVEEMQKLNQ--ESSAAELLNFANRHLSLVDATSRAFINRSRATSAAKS 552 +++ TE+ EE+ + E AE A L +D F++ ++ Sbjct: 478 LSI------TELTEEILDKTEYREMLKAEKSIEAQSRLENIDE----FLSVTKNFEQKSE 527 Query: 553 IQTFIAFATRHPEMNAADFLAELHRRQQKVSKNRNAIQSRRQLTLTTIQAAKGRQWPHVL 612 +T +AF T + D L + +++ S ++AI TL T+ AAKG ++P V Sbjct: 528 DKTLVAFLTDLALIADIDQLDQ----KEEESGGKDAI------TLMTLHAAKGLEFPVVF 577 Query: 613 VPYLQQHEFPRLRDIAEEV------RHFYVAMTRAISSLSL 647 + L++ FP R + EE R YV +TRA L L Sbjct: 578 LMGLEEGVFPHSRSLMEEAEMEEERRLAYVGITRAEQELYL 618 >jhp1371 [L] COG0210 Superfamily I DNA and RNA helicases jHp Helicobacter pylori J99 Length = 681 Score = 75.5 bits (184), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 111/429 (25%), Positives = 176/429 (41%), Gaps = 66/429 (15%) Query: 241 LIDEWHDINAAEFRLL-QLLLRGNKLVAVGDRYQIINSTRGADIVYSTAGFEMAFPNAQY 299 ++DE+ D NA + L QL + L VGD Q I RGADI + F F A+ Sbjct: 202 MVDEYQDTNALQLEFLKQLSFTHHNLCVVGDDDQSIYGFRGADIS-NILNFSKHFKGAKI 260 Query: 300 LPLGKSYRFGASLSKLAQRLTARKCASHEDLYTEIKR-----LTYDPTLSGNCAAKVTQQ 354 + L +YR A + A L + H K + + + V Q Sbjct: 261 VKLETNYRSSAEILACANSLISHNQHRHIKTLQSFKGSHKSVICKEYPTQKEESLDVAYQ 320 Query: 355 VQALVKARADTRYSDIAIIVREPDQTIEIENLLLDQNIPYYCDDIESYLHRPEILMLRGL 414 ++AL+K + +IAI+ R + IE L NIPY S+ R E+ L Sbjct: 321 IKALLKKGENLE--NIAILYRLNGLSRSIEESLNALNIPYRLIGAVSFYERAEVKDALAL 378 Query: 415 LHMASNTFATLQGDKESIKRMVMGFSLYLP---VGESSEDWERGYF-AEGSWFEEAQRMI 470 +H+ + + D+ IKR+ L P +G+ +++W EG EEA ++ Sbjct: 379 MHVVAK-----KDDRFFIKRV-----LNKPPRGLGKITQEWIFSLLDEEGLNLEEALKIG 428 Query: 471 A-------EQPNALKDFFEAVLCKPRDVDDDMTLRWKQRFTEVVEEMQKLNQESSAAELL 523 A + ALK F A++ + R+ + ++ +RF E ++ +E + E Sbjct: 429 AFKDKLNPKNEYALKK-FTAMIGRLREAFEISVEKFCERFLEETNLLKSYEKEDNYEERE 487 Query: 524 NFANRHLSLVDATSRAFINRSRATSAAKSIQTFIAFATRHPEMNAADFLAELHRRQQKVS 583 F LSLV F T +P + DFL E V Sbjct: 488 GFVKELLSLVKE----------------------HFKT-NPTHSLLDFLNE---SALDVH 521 Query: 584 KNRNAIQSRRQLTLTTIQAAKGRQWPHVLVPYLQQHEFP-----RLRDIAEEVRHFYVAM 638 NA ++++ ++ +KG ++ HV V L++ FP + D+ EE R YVA+ Sbjct: 522 NTENA----QKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGFNQESDLEEERRLAYVAI 577 Query: 639 TRAISSLSL 647 TRA L L Sbjct: 578 TRAKEELQL 586 >HP1478 [L] COG0210 Superfamily I DNA and RNA helicases Hpy Helicobacter pylori 26695 Length = 682 Score = 72.0 bits (175), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 111/438 (25%), Positives = 178/438 (40%), Gaps = 84/438 (19%) Query: 241 LIDEWHDINAAEFRLLQLL-LRGNKLVAVGDRYQIINSTRGADIVYSTAGFEMAFPNAQY 299 ++DE+ D NA + L+ L + L VGD Q I RGADI + F F A+ Sbjct: 203 MVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGFRGADIS-NILNFSKHFKGAKI 261 Query: 300 LPLGKSYRFGASLSKLAQRLTARKCASHEDLYTEIKRLTYDPTLSGN-----CAAKVTQ- 353 + L +YR A + A L + H IK L + G+ C +TQ Sbjct: 262 VKLETNYRSSAEILACANSLISHNQHRH------IKTLQ---SFKGSHKSVVCKEYLTQK 312 Query: 354 --------QVQALVKARADTRYSDIAIIVREPDQTIEIENLLLDQNIPYYCDDIESYLHR 405 Q++AL+K + +IAI+ R + IE L NIPY S+ R Sbjct: 313 EESLDVAYQIKALLKKGENLE--NIAILYRLNGLSRSIEESLNALNIPYRLIGALSFYER 370 Query: 406 PEILMLRGLLHMASNTFATLQGDKESIKRMVMGFSLYLP---VGESSEDWERGYF-AEGS 461 EI +H+ + + D+ IKR+ L P +G+ +++W EG Sbjct: 371 AEIKDALAFMHLVAK-----KDDRFFIKRV-----LNKPPRGLGKITQEWIFSLLDEEGL 420 Query: 462 WFEEAQRMIA-------EQPNALKDFFEAVLCKPRDVDDDMTLRWKQRFTEVVEEMQKLN 514 EEA ++ A + ALK F A++ + R+ + + RF E ++ Sbjct: 421 NLEEALKLGAFKDKLNPKNEYALKQFI-AMIGRLREAFEISVEEFCSRFLEETNLLKSYE 479 Query: 515 QESSAAELLNFANRHLSLVDATSRAFINRSRATSAAKSIQTFIAFATRHPEMNAADFLAE 574 +E + E F L+LV + +P + DFL E Sbjct: 480 KEDNYEEREGFVKELLTLVK-----------------------EYFKTNPTHSLLDFLNE 516 Query: 575 LHRRQQKVSKNRNAIQSRRQLTLTTIQAAKGRQWPHVLVPYLQQHEFP-----RLRDIAE 629 V N ++ ++++ ++ +KG ++ HV V L++ FP + D+ E Sbjct: 517 ------SVLDAHNT-ENAQKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGFNQESDLEE 569 Query: 630 EVRHFYVAMTRAISSLSL 647 E R YVA+TRA L L Sbjct: 570 ERRLAYVAITRAKEELQL 587