BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0163.p (324 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0322 [H] COG0320 Lipoate synthase Rso Ralstonia solanacearum 535 e-152 NMA1378 [H] COG0320 Lipoate synthase NmA Neisseria meningitidis ... 457 e-128 NMB1216 [H] COG0320 Lipoate synthase Nme Neisseria meningitidis ... 456 e-128 PA3996 [H] COG0320 Lipoate synthase Pae Pseudomonas aeruginosa 426 e-119 lipA [H] COG0320 Lipoate synthase Eco Escherichia coli K12 407 e-113 >RSc0322 [H] COG0320 Lipoate synthase Rso Ralstonia solanacearum Length = 333 Score = 535 bits (1379), Expect = e-152, Method: Compositional matrix adjust. Identities = 248/306 (81%), Positives = 276/306 (90%) Query: 14 NATDKQKGAGKTAWIPIKIMPAEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEE 73 +AT KQK KTA IPIKI+PAEKLKKP+WIRVKA + +RFYEIKDILRANNLVTVCEE Sbjct: 23 DATRKQKSLDKTARIPIKIVPAEKLKKPEWIRVKAATGNSRFYEIKDILRANNLVTVCEE 82 Query: 74 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSY 133 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLD NEPENLA+TIAQL+L+Y Sbjct: 83 ASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDANEPENLAKTIAQLRLNY 142 Query: 134 VVITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDV 193 VVITSVDRDDLRDGGA H+ CI R RELSP TRIE+L PDFRGR+++AL+IL+ PPDV Sbjct: 143 VVITSVDRDDLRDGGAQHYVDCISRTRELSPATRIEVLVPDFRGRLEKALDILQACPPDV 202 Query: 194 MNHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMR 253 MNHN+ET PRLYK+ARPG+DY +SL LLK FK +PN PTKSG+MVGLGETDEE+L+VMR Sbjct: 203 MNHNMETVPRLYKQARPGADYAHSLKLLKDFKARNPNLPTKSGLMVGLGETDEEILEVMR 262 Query: 254 DMRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYH 313 DMR H++DMLTIGQYL PSG HLPV RYVHPDTFKM+EE+AY+MGF HAAVGAMVRSSYH Sbjct: 263 DMREHDIDMLTIGQYLAPSGHHLPVLRYVHPDTFKMFEEKAYEMGFTHAAVGAMVRSSYH 322 Query: 314 ADQQAH 319 ADQQAH Sbjct: 323 ADQQAH 328 >NMA1378 [H] COG0320 Lipoate synthase NmA Neisseria meningitidis Z2491 Length = 327 Score = 457 bits (1176), Expect = e-128, Method: Compositional matrix adjust. Identities = 213/302 (70%), Positives = 258/302 (85%), Gaps = 3/302 (0%) Query: 18 KQKGAGKTAWIPIKIMP-AEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 76 K +GA KTA IPIK++P EKLKKP+WIR K P+ +F+EIKDILR + TVCEEASC Sbjct: 14 KLRGADKTARIPIKVVPLQEKLKKPEWIRAKL--PSRKFFEIKDILREQKMHTVCEEASC 71 Query: 77 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSYVVI 136 PNIGECF KGTATFMIMGD CTRRCPFCDVGHGRP+ LDP+EP+NLA ++ + L YVVI Sbjct: 72 PNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPKNLAESVKAMNLRYVVI 131 Query: 137 TSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 196 TSVDRDDLRDGGA HFA CI+ +RE SP T+IEIL PDFRGR+D AL+IL PPDVMNH Sbjct: 132 TSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKILAETPPDVMNH 191 Query: 197 NLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMRDMR 256 NLET P LY++ARPG++Y++SL+LLKR+K++ P+ PTKSGIMVGLGETDE+V ++MRDMR Sbjct: 192 NLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETDEDVREIMRDMR 251 Query: 257 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 316 AHN++M+TIGQYL PS HLPV RYV P+ FK++E+EAY++GF +AA+GAMVRSSYHAD+ Sbjct: 252 AHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADE 311 Query: 317 QA 318 QA Sbjct: 312 QA 313 >NMB1216 [H] COG0320 Lipoate synthase Nme Neisseria meningitidis MC58 Length = 327 Score = 456 bits (1174), Expect = e-128, Method: Compositional matrix adjust. Identities = 213/302 (70%), Positives = 257/302 (85%), Gaps = 3/302 (0%) Query: 18 KQKGAGKTAWIPIKIMP-AEKLKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 76 K +GA KTA IPIK++P EKLKKP+WIR K P+ +F+EIKDILR + TVCEEASC Sbjct: 14 KLRGADKTARIPIKVVPLQEKLKKPEWIRAKL--PSRKFFEIKDILREQKMHTVCEEASC 71 Query: 77 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSYVVI 136 PNIGECF KGTATFMIMGD CTRRCPFCDVGHGRP+ LDP+EP NLA ++ + L YVVI Sbjct: 72 PNIGECFSKGTATFMIMGDICTRRCPFCDVGHGRPNMLDPDEPRNLAESVKAMNLRYVVI 131 Query: 137 TSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 196 TSVDRDDLRDGGA HFA CI+ +RE SP T+IEIL PDFRGR+D AL+IL PPDVMNH Sbjct: 132 TSVDRDDLRDGGAQHFADCIKAIRETSPNTKIEILVPDFRGRLDIALKILAETPPDVMNH 191 Query: 197 NLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMRDMR 256 NLET P LY++ARPG++Y++SL+LLKR+K++ P+ PTKSGIMVGLGETDE+V ++MRDMR Sbjct: 192 NLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKSGIMVGLGETDEDVREIMRDMR 251 Query: 257 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 316 AHN++M+TIGQYL PS HLPV RYV P+ FK++E+EAY++GF +AA+GAMVRSSYHAD+ Sbjct: 252 AHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADE 311 Query: 317 QA 318 QA Sbjct: 312 QA 313 >PA3996 [H] COG0320 Lipoate synthase Pae Pseudomonas aeruginosa Length = 327 Score = 426 bits (1096), Expect = e-119, Method: Compositional matrix adjust. Identities = 201/304 (66%), Positives = 244/304 (80%), Gaps = 2/304 (0%) Query: 18 KQKGAGKTAWIPIKIMPAEKL-KKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEASC 76 K +GA K A IP+KI+P E+L KKPDWIRV+ + IK +LR + L +VCEEASC Sbjct: 21 KLRGAEKVARIPVKIIPTEELPKKPDWIRVRIPV-SPEVDRIKQLLRKHKLHSVCEEASC 79 Query: 77 PNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSYVVI 136 PN+GECF GTATFMIMGD CTRRCPFCDVGHGRP PLD +EP NLA IA L+L YVVI Sbjct: 80 PNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVDEPTNLAIAIADLRLKYVVI 139 Query: 137 TSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVMNH 196 TSVDRDDLRDGGA HFA C++ +R+LSP ++E L PD+RGRMD ALEI PPDV NH Sbjct: 140 TSVDRDDLRDGGAQHFADCLREIRKLSPGIQLETLVPDYRGRMDIALEITANEPPDVFNH 199 Query: 197 NLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMRDMR 256 NLET PRLY+ +RPGSD+E+SL+LL++FK + P+ PTKSG+M+GLGETD+EV++VM+ MR Sbjct: 200 NLETVPRLYRSSRPGSDFEWSLDLLQKFKQMVPHVPTKSGLMLGLGETDDEVIEVMQRMR 259 Query: 257 AHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHADQ 316 H++DMLT+GQYL PS +HLPV+R+VHPDTF + EE KMGF + A G +VRSSYHADQ Sbjct: 260 EHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGEKMGFKNVASGPLVRSSYHADQ 319 Query: 317 QAHG 320 QAHG Sbjct: 320 QAHG 323 >lipA [H] COG0320 Lipoate synthase Eco Escherichia coli K12 Length = 321 Score = 407 bits (1046), Expect = e-113, Method: Compositional matrix adjust. Identities = 192/307 (62%), Positives = 230/307 (74%), Gaps = 3/307 (0%) Query: 18 KQKGAGKTAWIPIKIMPAEK---LKKPDWIRVKAGSPTTRFYEIKDILRANNLVTVCEEA 74 K + A K A IP+K + E+ L+KP+W+++K + +TR IK +R N L +VCEEA Sbjct: 12 KYRDADKMALIPVKNVATEREALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEA 71 Query: 75 SCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDPNEPENLARTIAQLKLSYV 134 SCPN+ ECF GTATFMI+G CTRRCPFCDV HGRP D NEP LA+TIA + L YV Sbjct: 72 SCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDANEPVKLAQTIADMALRYV 131 Query: 135 VITSVDRDDLRDGGAGHFAACIQRVRELSPETRIEILTPDFRGRMDRALEILKMAPPDVM 194 VITSVDRDDLRDGGA HFA CI +RE SP+ +IE L PDFRGRMDRAL+IL PPDV Sbjct: 132 VITSVDRDDLRDGGAQHFADCITAIREKSPQIKIETLVPDFRGRMDRALDILTATPPDVF 191 Query: 195 NHNLETAPRLYKEARPGSDYEYSLNLLKRFKDLHPNTPTKSGIMVGLGETDEEVLQVMRD 254 NHNLE PR+Y++ RPG+DY +SL LL+RFK+ HP PTKSG+MVGLGET+EE+++VMRD Sbjct: 192 NHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRD 251 Query: 255 MRAHNVDMLTIGQYLMPSGDHLPVRRYVHPDTFKMYEEEAYKMGFVHAAVGAMVRSSYHA 314 +R H V MLT+GQYL PS HLPV+RYV PD F + EA MGF HAA G VRSSYHA Sbjct: 252 LRRHGVTMLTLGQYLQPSRHHLPVQRYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHA 311 Query: 315 DQQAHGL 321 D QA G+ Sbjct: 312 DLQAKGM 318