BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0050.p (321 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC320.14 [E] COG1171 Threonine dehydratase Spo Schizosaccharom... 436 e-122 PA2683 [E] COG1171 Threonine dehydratase Pae Pseudomonas aeruginosa 426 e-119 YKL218c [E] COG1171 Threonine dehydratase Sce Saccharomyces cere... 409 e-114 RSp1516 [E] COG1171 Threonine dehydratase Rso Ralstonia solanace... 409 e-114 CC3105 [E] COG1171 Threonine dehydratase Ccr Caulobacter vibrioides 253 3e-67 >SPCC320.14 [E] COG1171 Threonine dehydratase Spo Schizosaccharomyces pombe Length = 323 Score = 436 bits (1122), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/316 (67%), Positives = 265/316 (83%) Query: 4 LPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNALA 63 LP Y+DV +A+ RI A+KTPVLTS T + E A+VFFKCENFQ+MGAFKFRGA+NAL+ Sbjct: 7 LPTYDDVASASERIKKFANKTPVLTSSTVNKEFVAEVFFKCENFQKMGAFKFRGALNALS 66 Query: 64 RFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVRF 123 + QR GV+TFSSGNHAQAIAL+A LG+PA I+MP DAPEAKVAAT+GYGG+V+ + Sbjct: 67 QLNEAQRKAGVLTFSSGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVIMY 126 Query: 124 NRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLGGGG 183 +RY ++RE++ ++E+ GLT+IPPYDHP V++GQGTAAKELFEE GPLDALFVCLGGGG Sbjct: 127 DRYKDDREKMAKEISEREGLTIIPPYDHPHVLAGQGTAAKELFEEVGPLDALFVCLGGGG 186 Query: 184 LLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVGHYT 243 LLSGS LAA+ +P+C+ YGVEPEAG+D QS R G I+HIDTPKTIADGAQTQH+G+YT Sbjct: 187 LLSGSALAARHFAPNCEVYGVEPEAGNDGQQSFRKGSIVHIDTPKTIADGAQTQHLGNYT 246 Query: 244 FEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRIGVI 303 F II+ V DILTVSD+EL+ C++FYA RMK++VEPTGCLSFAA R MK++LK KRIG+I Sbjct: 247 FSIIKEKVDDILTVSDEELIDCLKFYAARMKIVVEPTGCLSFAAARAMKEKLKNKRIGII 306 Query: 304 VSGGNVDLAKLSGYFA 319 +SGGNVD+ + + + + Sbjct: 307 ISGGNVDIERYAHFLS 322 >PA2683 [E] COG1171 Threonine dehydratase Pae Pseudomonas aeruginosa Length = 320 Score = 426 bits (1094), Expect = e-119, Method: Compositional matrix adjust. Identities = 219/314 (69%), Positives = 267/314 (85%) Query: 1 MPELPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMN 60 M +LP Y+DV+AAA RIAGHA++TPV++SRT D E+GA+VFFKCEN QRMGAFKFRGA N Sbjct: 1 MHDLPTYDDVIAAAARIAGHANRTPVMSSRTLDEELGAEVFFKCENLQRMGAFKFRGAFN 60 Query: 61 ALARFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKV 120 AL+RF+ EQR GVV FSSGNHAQAIAL+A LG+PATIVMP DAP K+ ATRGYGG+V Sbjct: 61 ALSRFSAEQRAAGVVAFSSGNHAQAIALSARLLGIPATIVMPADAPAVKIEATRGYGGQV 120 Query: 121 VRFNRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLG 180 V ++RYTE+RE+I LA++HGLT+IPPYDHPDV++GQGTAAKELFEE GPLDA F LG Sbjct: 121 VLYDRYTEDREQIGRDLAQRHGLTLIPPYDHPDVLAGQGTAAKELFEEVGPLDAFFAPLG 180 Query: 181 GGGLLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVG 240 GGGLLSG LA +AL+P C+ YGVEPEAG+D +SLR+G I+HIDTP+T+ADGAQTQH+G Sbjct: 181 GGGLLSGCALAIRALAPACRIYGVEPEAGNDGQRSLRSGAIVHIDTPQTLADGAQTQHLG 240 Query: 241 HYTFEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRI 300 + TF +I+R+V DILT SD ELV MRF A RMK++VEPTGCL AA R+ KD+L+GKR+ Sbjct: 241 NLTFPLIQRNVDDILTASDAELVDGMRFLAARMKLLVEPTGCLGLAAARQRKDELRGKRV 300 Query: 301 GVIVSGGNVDLAKL 314 G+++SGGNVDLA+ Sbjct: 301 GILLSGGNVDLARF 314 >YKL218c [E] COG1171 Threonine dehydratase Sce Saccharomyces cerevisiae Length = 326 Score = 409 bits (1050), Expect = e-114, Method: Compositional matrix adjust. Identities = 204/310 (65%), Positives = 252/310 (81%) Query: 4 LPDYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNALA 63 +P Y DV+ A+ RI + +KTPVLTSR + +GAQ++FK ENFQR+GAFKFRGAMNA++ Sbjct: 3 VPTYGDVLDASNRIKEYVNKTPVLTSRMLNDRLGAQIYFKGENFQRVGAFKFRGAMNAVS 62 Query: 64 RFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVRF 123 + + E+R+ GV+ FSSGNHAQAIAL+A L +PATIVMPEDAP KVAAT GYG ++R+ Sbjct: 63 KLSDEKRSKGVIAFSSGNHAQAIALSAKLLNVPATIVMPEDAPALKVAATAGYGAHIIRY 122 Query: 124 NRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCLGGGG 183 NRYTE+RE+I LA +HG +IPPYDHPDVI+GQGT+AKEL EE G LDALFV LGGGG Sbjct: 123 NRYTEDREQIGRQLAAEHGFALIPPYDHPDVIAGQGTSAKELLEEVGQLDALFVPLGGGG 182 Query: 184 LLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHVGHYT 243 LLSGS LAA++LSP C+ +GVEPEAG+D QS R+G I+HI+TPKTIADGAQTQH+G YT Sbjct: 183 LLSGSALAARSLSPGCKIFGVEPEAGNDGQQSFRSGSIVHINTPKTIADGAQTQHLGEYT 242 Query: 244 FEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRIGVI 303 F IIR +V DILTVSD ELV CM F AERMK++VEPT CL FA K++L GK++G+I Sbjct: 243 FAIIRENVDDILTVSDQELVKCMHFLAERMKVVVEPTACLGFAGALLKKEELVGKKVGII 302 Query: 304 VSGGNVDLAK 313 +SGGNVD+ + Sbjct: 303 LSGGNVDMKR 312 >RSp1516 [E] COG1171 Threonine dehydratase Rso Ralstonia solanacearum Length = 323 Score = 409 bits (1050), Expect = e-114, Method: Compositional matrix adjust. Identities = 219/316 (69%), Positives = 258/316 (81%), Gaps = 1/316 (0%) Query: 1 MPELP-DYNDVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAM 59 M LP Y+DVVAA R+ G H+TPVLTS TA+A GA++FFKCENFQRMGAFKFRGA Sbjct: 1 MTTLPITYDDVVAAHARLQGQVHRTPVLTSTTANALTGAELFFKCENFQRMGAFKFRGAY 60 Query: 60 NALARFTPEQRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGK 119 NAL++FTPEQR GV+TFSSGNHAQAIAL+A LGM A IVMP+DAP KV ATRGYGG+ Sbjct: 61 NALSQFTPEQRKAGVITFSSGNHAQAIALSARLLGMRAVIVMPKDAPTIKVEATRGYGGE 120 Query: 120 VVRFNRYTENREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEETGPLDALFVCL 179 VV ++RYTE+RE I LAE+HGLT+IPPYDHP V++GQGTAAKEL EETGPLD L VCL Sbjct: 121 VVFYDRYTEDREAIGRRLAEQHGLTLIPPYDHPHVMAGQGTAAKELIEETGPLDLLLVCL 180 Query: 180 GGGGLLSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDTPKTIADGAQTQHV 239 GGGGLLSG AA+ALSP C+ GVEPEAG+D QSLR G+I+HIDTP TIADGAQTQH+ Sbjct: 181 GGGGLLSGCATAARALSPACRIIGVEPEAGNDGQQSLRKGEIVHIDTPATIADGAQTQHL 240 Query: 240 GHYTFEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKR 299 GHYTF +IR V DI TVSD +LV MRF+A RMK++VEPTGCL+ AA + + +++GKR Sbjct: 241 GHYTFAVIRALVDDIATVSDADLVDTMRFFAGRMKIVVEPTGCLAAAAALRQRVEVRGKR 300 Query: 300 IGVIVSGGNVDLAKLS 315 +GVI+SGGNVDL + Sbjct: 301 VGVIISGGNVDLQHFA 316 >CC3105 [E] COG1171 Threonine dehydratase Ccr Caulobacter vibrioides Length = 325 Score = 253 bits (647), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 128/307 (41%), Positives = 192/307 (62%), Gaps = 5/307 (1%) Query: 9 DVVAAAGRIAGHAHKTPVLTSRTADAEIGAQVFFKCENFQRMGAFKFRGAMNALARFTPE 68 D+ AAA R+ G A +TP++ S + +G ++F K E QR GAFKFRGA N L++ + E Sbjct: 7 DIQAAAVRLKGSAVETPLIESPALNDRLGGRIFLKPETLQRAGAFKFRGAYNRLSQLSDE 66 Query: 69 QRNDGVVTFSSGNHAQAIALAAHELGMPATIVMPEDAPEAKVAATRGYGGKVVRFNRYTE 128 ++ GVV FSSGNHAQ +ALAA LG+PA IVMP D+P KV TRG+G + ++R+TE Sbjct: 67 EKARGVVAFSSGNHAQGVALAARLLGVPALIVMPSDSPSVKVEGTRGFGADIRFYDRFTE 126 Query: 129 NREEICTALAEKHGLTMIPPYDHPDVISGQGTAAKELFEET----GPLDALFVCLGGGGL 184 +R I +A + G ++P YD P +I+GQGT E+ + LD L C+GGGGL Sbjct: 127 DRVAIADQIAAERGCVVVPSYDDPHIIAGQGTVGLEIVAQAAAQGATLDRLICCVGGGGL 186 Query: 185 LSGSLLAAKALSPDCQTYGVEPEAGDDALQSLRAGKIIHIDT-PKTIADGAQTQHVGHYT 243 ++G+ A KALSP + +GVEP D+ +SL +G+ ID ++I D T G T Sbjct: 187 IAGTSTAVKALSPATEIWGVEPAGFDETRRSLESGRRETIDKDARSICDALLTPIPGDLT 246 Query: 244 FEIIRRDVADILTVSDDELVACMRFYAERMKMIVEPTGCLSFAAVRKMKDQLKGKRIGVI 303 + I +++++ ++ V+D E+ MR+ +K++VEP GC++ A K + GK + ++ Sbjct: 247 WPINQKNLSGVVAVTDAEVAEAMRYAFSTLKLVVEPGGCVALTAALTGKVDVAGKTVAIV 306 Query: 304 VSGGNVD 310 +SGGNVD Sbjct: 307 LSGGNVD 313