BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0026.p (375 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2043 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific P... 302 7e-82 NMA1085 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific ... 300 4e-81 NMB0867 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific ... 300 5e-81 Rv3300c [J] COG0564 Pseudouridylate synthases, 23S RNA-specific ... 212 1e-54 MT3399 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific M... 210 3e-54 >PA2043 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific Pae Pseudomonas aeruginosa Length = 300 Score = 302 bits (774), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 149/274 (54%), Positives = 195/274 (71%), Gaps = 2/274 (0%) Query: 99 AGVWNNMLDFLTARFPGVTEEAWRQRMARGEVVNQDGVALAPDSAYRRGDCLFYYRDLEG 158 AG W+ +LD L ARFP + + W RM RG V++ +G AL P +AYR G + Y+R+L Sbjct: 20 AGPWSTVLDCLCARFPAIDRDTWLARMQRGRVLDAEGRALGPQAAYREGLRVHYFRELPV 79 Query: 159 GEAPIPFTEQILHQDAHLLVVDKPHFVPVIPTGRFLHESLLVRLKKKTGLADLTPIHRLD 218 EAPIPF +IL++D HLLV DKPHF+PV+P G ++ ++LL RL + TG DL P+HR+D Sbjct: 80 -EAPIPFEARILYRDEHLLVADKPHFLPVMPAGGYVEQTLLARLARSTGNRDLVPLHRID 138 Query: 219 RETAGVIIFSHNPASRGAYQAMFQQRTMDKTYEALAGLLEGRSFPFTHRSRMVDADKFFL 278 R TAG+++FS NP SRG YQA+F++R +DK YEA+A L FP R+R+V + FF Sbjct: 139 RHTAGLVLFSTNPGSRGRYQALFRERRIDKCYEAIAPALPQLDFPLLRRTRLVPGEPFFR 198 Query: 279 MREEEGAPNSETHVDLIERRGALGLYRLNPVTGRKHQLRVHMAALGIPIVNDNFYPHVVP 338 MRE EG PNSET ++++ER G YRL PVTG+KHQLRVH+AALG I ND FYP ++ Sbjct: 199 MREGEGEPNSETRIEVVERNGRWWRYRLYPVTGKKHQLRVHLAALGAGIQNDGFYPELLD 258 Query: 339 A-GTADNYDAPLKLLARSIAFTDPLSGDVRYFES 371 A G+ D+Y PLKLLAR + F DPLSG+ R FES Sbjct: 259 AEGSPDDYLRPLKLLARGLCFDDPLSGERRMFES 292 >NMA1085 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific NmA Neisseria meningitidis Z2491 Length = 307 Score = 300 bits (767), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 159/298 (53%), Positives = 197/298 (66%), Gaps = 8/298 (2%) Query: 81 PLPLKDGVAPSYLWLQDAAGVWN-NMLDFLTARFPGVTEEAWRQRMARGEVVNQDGVALA 139 PLPL +GV PSYL L + +L FL RFP V + WR+R+ G VV DG AL Sbjct: 7 PLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVGADDWRRRLNSGFVVGSDGAALD 66 Query: 140 PDSAYRRGDCLFYYRDLEGGEAP-IPFTEQILHQDAHLLVVDKPHFVPVIPTGRFLHESL 198 S + G +FYYR+ P IPF E+ILH D HL+VVDKPHF+PVIP+GRFL E+L Sbjct: 67 EHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDEHLIVVDKPHFLPVIPSGRFLRETL 126 Query: 199 LVRLK-----KKTGLADLTPIHRLDRETAGVIIFSHNPASRGAYQAMFQQRTMDKTYEAL 253 L RL+ + + D+TP+HRLD++TAGV++ SHNPA+RGAYQ MFQ +T+ KTYEAL Sbjct: 127 LTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLLSHNPATRGAYQTMFQNKTVWKTYEAL 186 Query: 254 AGLLEGRSFPFTHRSRMVDADKFFLMREEEGAPNSETHVDLIERRGALGLYRLNPVTGRK 313 A +P SR+V +KFF +E EG PN+ T V+LIE RG LYRL P TG+K Sbjct: 187 APTRTDLPYPLDVVSRLVRGEKFFTTQEAEGEPNAHTTVELIENRGEFSLYRLTPHTGKK 246 Query: 314 HQLRVHMAALGIPIVNDNFYPHVVPAGTADNYDAPLKLLARSIAFTDPLSGDVRYFES 371 HQLRVHM LG+P++ND YP AG+ D Y PLKLLA+ IAF DPLSG R F S Sbjct: 247 HQLRVHMMGLGMPLLNDALYPVPSEAGSED-YRKPLKLLAKKIAFADPLSGRERVFCS 303 >NMB0867 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific Nme Neisseria meningitidis MC58 Length = 307 Score = 300 bits (767), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 159/298 (53%), Positives = 197/298 (66%), Gaps = 8/298 (2%) Query: 81 PLPLKDGVAPSYLWLQDAAGVWN-NMLDFLTARFPGVTEEAWRQRMARGEVVNQDGVALA 139 PLPL +GV PSYL L + +L FL RFP V + WR+R+ G VV DG AL Sbjct: 7 PLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVGADDWRRRLNSGFVVGSDGAALD 66 Query: 140 PDSAYRRGDCLFYYRDLEGGEAP-IPFTEQILHQDAHLLVVDKPHFVPVIPTGRFLHESL 198 S + G +FYYR+ P IPF E+ILH D HL+VVDKPHF+PVIP+GRFL E+L Sbjct: 67 EHSLFEPGKVVFYYRETSRESEPRIPFEEKILHIDEHLIVVDKPHFLPVIPSGRFLRETL 126 Query: 199 LVRLK-----KKTGLADLTPIHRLDRETAGVIIFSHNPASRGAYQAMFQQRTMDKTYEAL 253 L RL+ + + D+TP+HRLD++TAGV++ SHNPA+RGAYQ MFQ +T+ KTYEAL Sbjct: 127 LTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLLSHNPATRGAYQTMFQNKTVWKTYEAL 186 Query: 254 AGLLEGRSFPFTHRSRMVDADKFFLMREEEGAPNSETHVDLIERRGALGLYRLNPVTGRK 313 A +P SR+V +KFF +E EG PN+ T V+LIE RG LYRL P TG+K Sbjct: 187 APTRTDLPYPLDVVSRLVRGEKFFTTQEAEGEPNAHTTVELIENRGEFSLYRLTPHTGKK 246 Query: 314 HQLRVHMAALGIPIVNDNFYPHVVPAGTADNYDAPLKLLARSIAFTDPLSGDVRYFES 371 HQLRVHM LG+P++ND YP AG+ D Y PLKLLA+ IAF DPLSG R F S Sbjct: 247 HQLRVHMMGLGMPLLNDALYPVPSEAGSED-YRKPLKLLAKKIAFADPLSGRERVFCS 303 >Rv3300c [J] COG0564 Pseudouridylate synthases, 23S RNA-specific Mtu Mycobacterium tuberculosis H37Rv Length = 305 Score = 212 bits (539), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 129/275 (46%), Positives = 171/275 (62%), Gaps = 9/275 (3%) Query: 101 VWNNMLDFLTARFPGVTEEAWRQRMARGEVVNQDGVALAPDSAYRRGDCLFYYRDLEGGE 160 + ++L LTARF GV A R ++ GEVV+ DG + + G + YRDL E Sbjct: 24 LGGSVLAELTARF-GV---AARAKVLAGEVVDDDGAVVDSGTVLPPGSVVHLYRDLPD-E 78 Query: 161 APIPFTEQILHQDAHLLVVDKPHFVPVIPTGRFLHESLLVRLKKKTGLADLTPIHRLDRE 220 P+PF +LHQDA ++VVDKPHF+ +P GR + ++ LVRL+++ GL +L+P HRLDR Sbjct: 79 VPVPFDVPVLHQDADIVVVDKPHFLATMPRGRHVAQTALVRLRRELGLPELSPAHRLDRL 138 Query: 221 TAGVIIFSHNPASRGAYQAMFQQRTMDKTYEALAGLLEGRSFPFTHRSRMVDADKFFLMR 280 TAGV++F+ RG+YQ MF + + KTY A A + G + P RSR+V Sbjct: 139 TAGVLLFTTRREVRGSYQTMFARGLVRKTYLARAPVAPGLALPRLVRSRIVKRRGHLQAV 198 Query: 281 EEEGAPNSETHVDLIERRGALGLYRLNPVTGRKHQLRVHMAALGIPIVNDNFYPHVVPAG 340 E G PN+ET L+ER GLYRL P TGR HQLRVHMAALGIPI+ D YP+V+ Sbjct: 199 CEPGVPNAET---LVERIARDGLYRLTPTTGRTHQLRVHMAALGIPIMGDPLYPNVISVA 255 Query: 341 TADNYDAPLKLLARSIAFTDPLSGDVRYFESLRTL 375 A ++ PL+LLA+ I F DPL+G R F S RTL Sbjct: 256 -AHDFSTPLQLLAQRIEFDDPLTGSHREFASTRTL 289 >MT3399 [J] COG0564 Pseudouridylate synthases, 23S RNA-specific MtC Mycobacterium tuberculosis CDC1551 Length = 305 Score = 210 bits (535), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 129/275 (46%), Positives = 170/275 (61%), Gaps = 9/275 (3%) Query: 101 VWNNMLDFLTARFPGVTEEAWRQRMARGEVVNQDGVALAPDSAYRRGDCLFYYRDLEGGE 160 + ++L LTARF GV A R ++ GEVV+ DG + + G + YRDL E Sbjct: 24 LGGSVLAELTARF-GV---AARAKVLAGEVVDDDGAVVDSGTVLPPGSVVHLYRDLPD-E 78 Query: 161 APIPFTEQILHQDAHLLVVDKPHFVPVIPTGRFLHESLLVRLKKKTGLADLTPIHRLDRE 220 P+PF +LHQDA ++VVDKPHF+ +P GR + ++ LVRL+++ GL +L+P HRLDR Sbjct: 79 VPVPFDVPVLHQDADIVVVDKPHFLATMPRGRHVAQTALVRLRRELGLPELSPAHRLDRL 138 Query: 221 TAGVIIFSHNPASRGAYQAMFQQRTMDKTYEALAGLLEGRSFPFTHRSRMVDADKFFLMR 280 TAGV++F+ RG+YQ MF + + KTY A A + G + P RSR+V Sbjct: 139 TAGVLLFTTRREVRGSYQTMFARGLVRKTYLARAPVAPGLALPRLVRSRIVKRRGHLQAV 198 Query: 281 EEEGAPNSETHVDLIERRGALGLYRLNPVTGRKHQLRVHMAALGIPIVNDNFYPHVVPAG 340 E G PN+ET L+ER GLYRL P TGR HQLRVHMAALGIPI+ D YP+V+ Sbjct: 199 CEPGVPNAET---LVERIARDGLYRLTPTTGRTHQLRVHMAALGIPIMGDPLYPNVISVA 255 Query: 341 TADNYDAPLKLLARSIAFTDPLSGDVRYFESLRTL 375 A + PL+LLA+ I F DPL+G R F S RTL Sbjct: 256 -AHYFSTPLQLLAQRIEFDDPLTGSHREFASTRTL 289