BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0249.p (434 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1220 [C] COG1251 NAD(P)H-nitrite reductase Rso Ralstonia sola... 533 e-151 PA1781 [C] COG1251 NAD(P)H-nitrite reductase Pae Pseudomonas aer... 395 e-109 BH0613 [C] COG1251 NAD(P)H-nitrite reductase Bha Bacillus halodu... 352 7e-97 BS_nasD [C] COG1251 NAD(P)H-nitrite reductase Bsu Bacillus subtilis 348 1e-95 AGl1891 [C] COG1251 NAD(P)H-nitrite reductase Atu Agrobacterium ... 345 1e-94 >RSp1220 [C] COG1251 NAD(P)H-nitrite reductase Rso Ralstonia solanacearum Length = 409 Score = 533 bits (1372), Expect = e-151, Method: Compositional matrix adjust. Identities = 259/314 (82%), Positives = 285/314 (90%), Gaps = 1/314 (0%) Query: 8 VASAKKPSLVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKS 67 ++ A +P LVVIGNGMAGMRTVEELLKLAPDLYDITVFG EPHGNYNRILLSPVLAG+K Sbjct: 1 MSRAARPKLVVIGNGMAGMRTVEELLKLAPDLYDITVFGEEPHGNYNRILLSPVLAGEKR 60 Query: 68 IDDIMLNTRDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIP 127 +DDIMLNTR+WY ++ ITLHAGDPV IDR RRTVRS+ GVE++YDRLL+ATGS+PFI+P Sbjct: 61 VDDIMLNTREWYEEHGITLHAGDPVVAIDRPRRTVRSRGGVEVRYDRLLMATGSRPFILP 120 Query: 128 VPGHELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVT 187 VPGH LPGVIAFRDIQDV MLD ARNHRHAVVIGGGLLGLEAANGL+RQGM+ TVVH++ Sbjct: 121 VPGHMLPGVIAFRDIQDVETMLDAARNHRHAVVIGGGLLGLEAANGLMRQGMEATVVHIS 180 Query: 188 DSLMNQQLDKPAAALLKKALEMKGLRFLLNAQTEAIVGPDRVTAVRFKDGSEIPADLVVM 247 D+LM +QLDKPAAALLK ALE KGLRFLL AQT ++G DRVTAVRFKDG+EIPADLVVM Sbjct: 181 DTLMERQLDKPAAALLKVALEAKGLRFLLGAQTTELIGTDRVTAVRFKDGTEIPADLVVM 240 Query: 248 TAGVRPNIGLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQAR 307 TAGVRPNI LA+ AGLHC+RAIVVDDTLQTYDPRIYAVGECVQHR ATFGLVAPIWDQAR Sbjct: 241 TAGVRPNIELARSAGLHCERAIVVDDTLQTYDPRIYAVGECVQHRSATFGLVAPIWDQAR 300 Query: 308 VCGAHLAGGSGWRR 321 VC AHLA G+G RR Sbjct: 301 VCAAHLA-GAGHRR 313 >PA1781 [C] COG1251 NAD(P)H-nitrite reductase Pae Pseudomonas aeruginosa Length = 816 Score = 395 bits (1014), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/305 (62%), Positives = 237/305 (77%), Gaps = 2/305 (0%) Query: 12 KKPSLVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDI 71 KK LV++GNGMAG+RT+EELLKL D YDITVFGAEPH NYNRILLSPVLAG+++ ++I Sbjct: 2 KKIKLVMVGNGMAGVRTLEELLKLNSDFYDITVFGAEPHPNYNRILLSPVLAGEQTFEEI 61 Query: 72 MLNTRDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIPVPGH 131 +LN +WY +N I L V +IDR RR V + G E +YDRLLLATGS PFI+P+PG+ Sbjct: 62 VLNDLNWYAENGIKLLLDRKVVQIDRLRRRVVAADGSEAEYDRLLLATGSLPFILPIPGN 121 Query: 132 ELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDSLM 191 L GVI +RDI D M+D AR H HAVVIGGGLLGLEAANGL ++GMDVTVVH++D L+ Sbjct: 122 RLQGVIGYRDIADTQAMIDCARTHSHAVVIGGGLLGLEAANGLKQRGMDVTVVHLSDWLL 181 Query: 192 NQQLDKPAAALLKKALEMKGLRFLLNAQTEAIV--GPDRVTAVRFKDGSEIPADLVVMTA 249 +QLD+ A LL+ ALE +G+RF LN QT+ ++ G RV AV+F DG IPADLVVM A Sbjct: 182 ERQLDRTAGKLLQGALEARGIRFRLNTQTQELMDNGSGRVCAVQFNDGDVIPADLVVMAA 241 Query: 250 GVRPNIGLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVC 309 G+RPN LA+ AG+ C+R I+V+DTLQTYDPRIYAVGEC HR +GLVAP+++QA+VC Sbjct: 242 GIRPNTELAESAGIPCNRGILVNDTLQTYDPRIYAVGECANHRGIAYGLVAPLFEQAKVC 301 Query: 310 GAHLA 314 HLA Sbjct: 302 ANHLA 306 >BH0613 [C] COG1251 NAD(P)H-nitrite reductase Bha Bacillus halodurans Length = 807 Score = 352 bits (904), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 162/297 (54%), Positives = 224/297 (75%), Gaps = 1/297 (0%) Query: 13 KPSLVVIGNGMAGMRTVEELLKLAP-DLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDI 71 K LVV+GNGMAG+RTVEEL + + Y++TV G EPH NYNRILLS VLA + +++DI Sbjct: 2 KEKLVVVGNGMAGIRTVEELFERGGHERYEVTVVGEEPHVNYNRILLSSVLASEANMEDI 61 Query: 72 MLNTRDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIPVPGH 131 LN+ DWY +N++TL+ + V K+DR+++ V + G +I YD+++LATGS PF++P+PG Sbjct: 62 ELNSLDWYRENDVTLYRSEKVTKLDRKKKVVLTDKGQKIAYDKVILATGSHPFMLPLPGS 121 Query: 132 ELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDSLM 191 +L GVIAFR+I+D M++ A+N++ AVVIGGGLLG+EAA GLL GM+V VVH+ D LM Sbjct: 122 DLEGVIAFRNIEDCETMIETAKNYKKAVVIGGGLLGIEAARGLLNLGMEVEVVHIFDYLM 181 Query: 192 NQQLDKPAAALLKKALEMKGLRFLLNAQTEAIVGPDRVTAVRFKDGSEIPADLVVMTAGV 251 +QLD+ AA +L+ LE +G++FLL TEAI+G RV V+FKDG+E ADLVVM G+ Sbjct: 182 ERQLDRTAANILRTELENQGMKFLLEKHTEAIIGKGRVERVKFKDGTETRADLVVMAVGI 241 Query: 252 RPNIGLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARV 308 RPN +A +G+ +R IVVDD +QT DP +YAVGEC +HR +GLVAP+W+Q ++ Sbjct: 242 RPNHEVASASGIEVNRGIVVDDYMQTSDPDVYAVGECAEHRGMVYGLVAPLWEQGKI 298 >BS_nasD [C] COG1251 NAD(P)H-nitrite reductase Bsu Bacillus subtilis Length = 805 Score = 348 bits (893), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 167/303 (55%), Positives = 213/303 (70%) Query: 13 KPSLVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDIM 72 K LV++GNGMAG+R +EE+L +A D + IT+FGAEPH NYNRILLS VL GD I DI Sbjct: 3 KKQLVLVGNGMAGVRAIEEILSVAKDEFQITIFGAEPHPNYNRILLSKVLQGDTDIKDIT 62 Query: 73 LNTRDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIPVPGHE 132 LN DWY +NNI L+ + V K+D +TV + A YD L+LATGS PFI+P+PG + Sbjct: 63 LNDWDWYEENNIQLYTNETVIKVDTENKTVITDADRIQPYDELILATGSVPFILPIPGAD 122 Query: 133 LPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDSLMN 192 GV AFRDI+D ML ++ ++ A VIGGGLLGLEAA GLL GMDV+V+H+ LM Sbjct: 123 KKGVTAFRDIKDTDTMLAASKQYKKAAVIGGGLLGLEAARGLLNLGMDVSVIHLAPFLME 182 Query: 193 QQLDKPAAALLKKALEMKGLRFLLNAQTEAIVGPDRVTAVRFKDGSEIPADLVVMTAGVR 252 +QLD A LL+ LE +G+ FLL QTE IVG DRV +RFKDG+ I ADLVVM G+R Sbjct: 183 RQLDATAGRLLQNELEKQGMTFLLEKQTEEIVGDDRVEGLRFKDGTSIEADLVVMAVGIR 242 Query: 253 PNIGLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVCGAH 312 PN L E+G+ +R I+V+D +QT P IYAVGEC +HR +GLVAP+++QA+V H Sbjct: 243 PNTTLGAESGIPVNRGIIVNDYMQTEIPHIYAVGECAEHRGIAYGLVAPLYEQAKVLAKH 302 Query: 313 LAG 315 + G Sbjct: 303 MCG 305 >AGl1891 [C] COG1251 NAD(P)H-nitrite reductase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 838 Score = 345 bits (885), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 164/302 (54%), Positives = 219/302 (72%) Query: 16 LVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDIMLNT 75 LV+IGNGMA R +E L + AP LYD+T+F AEP NY+RI+LSPVL+G+KS +DI+++ Sbjct: 28 LVIIGNGMAPGRMLENLFETAPGLYDVTIFNAEPRVNYDRIMLSPVLSGEKSYEDIVIHN 87 Query: 76 RDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIPVPGHELPG 135 +WY NN+TLH G V +IDR + V S+ G+ YDRL++ATGS PFIIPVPGH+LPG Sbjct: 88 DEWYAANNVTLHKGAKVTRIDRANKIVTSENGITASYDRLVIATGSLPFIIPVPGHQLPG 147 Query: 136 VIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDSLMNQQL 195 V+A+RD+ DV ML++A+ A+VIG GLLGLEAA GL RQGMDVTV+H+ ++M +QL Sbjct: 148 VLAYRDLDDVTKMLEIAKGKGRAIVIGAGLLGLEAAYGLKRQGMDVTVIHLMPTIMERQL 207 Query: 196 DKPAAALLKKALEMKGLRFLLNAQTEAIVGPDRVTAVRFKDGSEIPADLVVMTAGVRPNI 255 D AA LL+KAL +G+ + A T+ I+G ++V + +DG I D+VVM G+RP Sbjct: 208 DPAAAYLLEKALNERGIDIITKANTKCILGEEKVEGIELEDGRIIKGDMVVMAVGIRPAS 267 Query: 256 GLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVCGAHLAG 315 GLAKEAG+ +R IVVDD + T D IYA+GEC +HR +GLVAP+++ ARV L G Sbjct: 268 GLAKEAGIAVNRGIVVDDGMMTSDASIYALGECAEHRGMCYGLVAPLYESARVLADRLCG 327 Query: 316 GS 317 GS Sbjct: 328 GS 329