BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0218.p (578 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0020 [P] COG0038 Chloride channel protein EriC Rso Ralstonia ... 437 e-122 YPO2270 [P] COG0038 Chloride channel protein EriC Ype Yersinia p... 272 2e-72 ynfJ [P] COG0038 Chloride channel protein EriC Eco Escherichia c... 223 1e-57 Z2583 [P] COG0038 Chloride channel protein EriC EcZ Escherichia ... 223 1e-57 ECs2298 [P] COG0038 Chloride channel protein EriC Ecs Escherichi... 223 1e-57 >RSp0020 [P] COG0038 Chloride channel protein EriC Rso Ralstonia solanacearum Length = 461 Score = 437 bits (1124), Expect = e-122, Method: Compositional matrix adjust. Identities = 240/431 (55%), Positives = 303/431 (70%), Gaps = 5/431 (1%) Query: 16 LRMSESHAMLLWAAVVGFVGALATIGFRECIDAVQYLLTGHSGSFVEIAKGLSWQMRVLL 75 L H +L A ++G GALATI FREC+ +Q+ L G V A+ L W R+L+ Sbjct: 25 LPFRREHLVLAIAGLIGCAGALATIAFRECLRQLQWWLAGADQGLVATARALPWWARLLV 84 Query: 76 PTCGGVVAGLILIWA-KRMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLATVGSGGSI 134 PT GG++AGL L + K +P DYMEA+A+GDG + R +L+RS SSL +V SG SI Sbjct: 85 PTAGGLLAGLTLQYGLKWIPRKGAEDYMEAIAVGDGVLSARQSLVRSASSLCSVASGASI 144 Query: 135 GREGPMVQLAALCASLVGRFAH--FPSS--RMRLLVACGAAAGITSAYNAPIAGAFFVTE 190 GREGPMVQLAA+C SL+GR P S MRLLVACGAAAGITSAYNAPIAGA FV E Sbjct: 145 GREGPMVQLAAMCGSLLGRVLRHAMPVSVEHMRLLVACGAAAGITSAYNAPIAGAVFVCE 204 Query: 191 IVLGALVMESFGPVVVASVVANITMRELPGYKPAYEMPYFPEVAGVEIILFVVLGVIAGL 250 IV GA+ + GP++V++V A+I +R+ GY Y MP+F V+G E++ ++ LG+ AG+ Sbjct: 205 IVFGAITTATLGPLLVSAVTADIIVRQFFGYGAVYAMPHFDFVSGWEVLTYLGLGLAAGM 264 Query: 251 LAPQFLRLLELSKTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVNSLLHTDW 310 P L L++ ++ AF +TRLP LRL LGGL+VG LS+RVPEVWGNGYSVVN LH W Sbjct: 265 AGPLLLGLIDRARGAFARTRLPQALRLALGGLIVGALSIRVPEVWGNGYSVVNGFLHAPW 324 Query: 311 LWSTVLIVMIYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWPHAISQPFA 370 LW TV +V++ K+ ATA + GSGAVGG+FTPTLF GAA+G L+G HAL P A P + Sbjct: 325 LWQTVALVLVCKVGATAASAGSGAVGGVFTPTLFCGAALGLLYGTGMHALLPGAAPVPVS 384 Query: 371 YAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDGNSMYEIT 430 YA+VGMGA LAA T+APLM+ILMIFEMTLSYQVVLPLML+C+ YV A + S+Y Sbjct: 385 YAVVGMGALLAATTHAPLMSILMIFEMTLSYQVVLPLMLACITGYVTAHATGAPSVYARA 444 Query: 431 LKRHRDAAERI 441 L R+RD R+ Sbjct: 445 LARNRDDTLRL 455 >YPO2270 [P] COG0038 Chloride channel protein EriC Ype Yersinia pestis Length = 431 Score = 272 bits (696), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 185/410 (45%), Positives = 257/410 (62%), Gaps = 18/410 (4%) Query: 30 VVGFVGALATIGFRECIDAVQYLLTGHS-GSFVEIAKGLSWQMRVLLPTCGGVVAGLILI 88 ++G V AL F + + +++LL + GS V A ++ R L P GG+ A +L+ Sbjct: 27 MLGMVSALIVWLFHQAMLGLEWLLFSRTDGSLVAAAASITGWRRALTPALGGLAA-GLLL 85 Query: 89 WA-----KRMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLATVGSGGSIGREGPMVQL 143 WA R P+ A +DYMEA+ IGDGR+ + +L++SL+SL V SG +IGREG MV L Sbjct: 86 WAYQRYQHRKPS-APTDYMEAIEIGDGRLDVSASLVKSLASLLVVSSGSAIGREGAMVLL 144 Query: 144 AALCASLVGRFAHFPSSRMRLLVACGAAAGITSAYNAPIAGAFFVTEIVLGALVMESFGP 203 AAL AS+ + P +L VACGAAAG+ SAY+AP+AG+ F+ EI+ G L++ S GP Sbjct: 145 AALFASVFAQRYAKPK-EWKLWVACGAAAGMASAYHAPLAGSLFIAEILFGTLMLASLGP 203 Query: 204 VVVASVVANITMRELPGYKPA-YEMPYFPEVAGVEIILFVVLGVIAGLLAPQFLRLLELS 262 VV+A+V A +T+ L G + Y++ P V+ L +LG++AG P FL+ + S Sbjct: 204 VVIAAVSALLTINLLQGGQETLYQVQTLPSPWPVQYFLMALLGLMAGFSGPLFLKAMAAS 263 Query: 263 KTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVNSLLHTDWLWSTVLI---VM 319 AFR L PL+L LGG++VGLLS+ PEVWGNGYSVV SLL T VL+ ++ Sbjct: 264 SHAFRSLNLLPPLQLALGGIIVGLLSLIFPEVWGNGYSVVQSLLTTP---PGVLLIGGIL 320 Query: 320 IYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWP-HAISQPFAYAMVGMGA 378 I K++A ++GSGA GG+FTPTLFVGAA+G L G + +LWP + A+ GM Sbjct: 321 ICKLLAVLASSGSGAPGGVFTPTLFVGAALGMLCGQI-FSLWPVLGDNIGLLMALTGMAT 379 Query: 379 FLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDGNSMYE 428 LAA T+AP+MA LM+ EMT Y ++ L+LSCV+A IAR L S+Y Sbjct: 380 LLAATTHAPIMAALMVCEMTGEYTLLPGLLLSCVIATTIARWLRPISVYR 429 >ynfJ [P] COG0038 Chloride channel protein EriC Eco Escherichia coli K12 Length = 438 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 173/417 (41%), Positives = 245/417 (58%), Gaps = 11/417 (2%) Query: 25 LLWAAVVGFVGALATIGFRECIDAVQYL-LTGHSGSFVEIAKGLSWQMRVLLPTCGGVVA 83 LL A VVG + A A GFR + +++L L SGS V A LS R+L P GG+ A Sbjct: 25 LLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSPWRRLLTPALGGLAA 84 Query: 84 GLILI-WAK--RMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLATVGSGGSIGREGPM 140 GL+L+ W K + A +DYMEA+ DG+ +L++SL+SL V SG +IGREG M Sbjct: 85 GLLLMGWQKFTQQRPHAPTDYMEALQT-DGQFDYAASLVKSLASLLVVTSGSAIGREGAM 143 Query: 141 VQLAALCASLVG-RFAHFPSSRMRLLVACGAAAGITSAYNAPIAGAFFVTEIVLGALVME 199 + LAAL AS RF P +L +ACGAAAG+ +AY AP+AG+ F+ E++ G +++ Sbjct: 144 ILLAALAASCFAQRFT--PRQEWKLWIACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLA 201 Query: 200 SFGPVVVASVVANITMRELPGYKPA--YEMPYFPEVAGVEIILFVVLGVIAGLLAPQFLR 257 S GPV++++VVA + + L + A Y + V + L + GV+AGL P L Sbjct: 202 SLGPVIISAVVA-LLVSNLINHSDALLYNVQLSVTVQARDYALIISTGVLAGLCGPLLLT 260 Query: 258 LLELSKTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVNSLLHTDWLWSTVLI 317 L+ F +L P +L LGGL+VGLLS+ P VWGNGYS V S L L + Sbjct: 261 LMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAG 320 Query: 318 VMIYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWPHAISQPFAYAMVGMG 377 + + K+ A ++GSGA GG+FTPTLF+G AIG L+G +P + GM Sbjct: 321 IFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMA 380 Query: 378 AFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDGNSMYEITLKRH 434 LAA T+AP+M+ LMI EMT YQ++ L+++CV+A VI+R+L +S+Y +H Sbjct: 381 TLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQH 437 >Z2583 [P] COG0038 Chloride channel protein EriC EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 175/431 (40%), Positives = 249/431 (57%), Gaps = 11/431 (2%) Query: 11 RLQTWLRMSESHAMLLWAAVVGFVGALATIGFRECIDAVQYL-LTGHSGSFVEIAKGLSW 69 L T+ + LL A VVG + A A GFR + +++L L SGS V A LS Sbjct: 11 HLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSP 70 Query: 70 QMRVLLPTCGGVVAGLILI-WAK--RMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLA 126 R+L P GG+ AGL+L+ W K + A +DYMEA+ DG+ +L++SL+SL Sbjct: 71 WRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQT-DGQFDYAASLVKSLASLL 129 Query: 127 TVGSGGSIGREGPMVQLAALCASLVG-RFAHFPSSRMRLLVACGAAAGITSAYNAPIAGA 185 V SG +IGREG M+ LAAL AS RF P +L +ACGAAAG+ +AY AP+AG+ Sbjct: 130 VVTSGSAIGREGAMILLAALAASCFAQRFT--PRQEWKLWIACGAAAGMAAAYRAPLAGS 187 Query: 186 FFVTEIVLGALVMESFGPVVVASVVANITMRELPGYKPA--YEMPYFPEVAGVEIILFVV 243 F+ E++ G +++ S GPV++++VVA + + L + A Y + V + L + Sbjct: 188 LFIAEVLFGTMMLASLGPVIISAVVA-LLVSNLINHSDALLYSVQLSVTVQARDYALIIS 246 Query: 244 LGVIAGLLAPQFLRLLELSKTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVN 303 GV+AGL P L L+ F +L P +L LGGL+VGLLS+ P VWGNGYS V Sbjct: 247 TGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQ 306 Query: 304 SLLHTDWLWSTVLIVMIYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWPH 363 S L L + + + K+ A ++GSGA GG+FTPTLF+G AIG L+G +P Sbjct: 307 SFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPD 366 Query: 364 AISQPFAYAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDG 423 + GM LAA T+AP+M+ LMI EMT YQ++ L+++CV+A VI+R+L Sbjct: 367 GEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHR 426 Query: 424 NSMYEITLKRH 434 +S+Y +H Sbjct: 427 DSIYRQHTAQH 437 >ECs2298 [P] COG0038 Chloride channel protein EriC Ecs Escherichia coli O157:H7 Length = 438 Score = 223 bits (567), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 175/431 (40%), Positives = 249/431 (57%), Gaps = 11/431 (2%) Query: 11 RLQTWLRMSESHAMLLWAAVVGFVGALATIGFRECIDAVQYL-LTGHSGSFVEIAKGLSW 69 L T+ + LL A VVG + A A GFR + +++L L SGS V A LS Sbjct: 11 HLHTYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWLFLNNDSGSLVNAATNLSP 70 Query: 70 QMRVLLPTCGGVVAGLILI-WAK--RMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLA 126 R+L P GG+ AGL+L+ W K + A +DYMEA+ DG+ +L++SL+SL Sbjct: 71 WRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQT-DGQFDYAASLVKSLASLL 129 Query: 127 TVGSGGSIGREGPMVQLAALCASLVG-RFAHFPSSRMRLLVACGAAAGITSAYNAPIAGA 185 V SG +IGREG M+ LAAL AS RF P +L +ACGAAAG+ +AY AP+AG+ Sbjct: 130 VVTSGSAIGREGAMILLAALAASCFAQRFT--PRQEWKLWIACGAAAGMAAAYRAPLAGS 187 Query: 186 FFVTEIVLGALVMESFGPVVVASVVANITMRELPGYKPA--YEMPYFPEVAGVEIILFVV 243 F+ E++ G +++ S GPV++++VVA + + L + A Y + V + L + Sbjct: 188 LFIAEVLFGTMMLASLGPVIISAVVA-LLVSNLINHSDALLYSVQLSVTVQARDYALIIS 246 Query: 244 LGVIAGLLAPQFLRLLELSKTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVN 303 GV+AGL P L L+ F +L P +L LGGL+VGLLS+ P VWGNGYS V Sbjct: 247 TGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVGLLSLFTPAVWGNGYSTVQ 306 Query: 304 SLLHTDWLWSTVLIVMIYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWPH 363 S L L + + + K+ A ++GSGA GG+FTPTLF+G AIG L+G +P Sbjct: 307 SFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPD 366 Query: 364 AISQPFAYAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDG 423 + GM LAA T+AP+M+ LMI EMT YQ++ L+++CV+A VI+R+L Sbjct: 367 GEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLHR 426 Query: 424 NSMYEITLKRH 434 +S+Y +H Sbjct: 427 DSIYRQHTAQH 437