BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0039.p (521 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0618_1 [H] COG1010 Precorrin-3B methylase Rso Ralstonia solan... 340 5e-93 RSp0618_2 [H] COG2099 Precorrin-6x reductase Rso Ralstonia solan... 297 4e-80 TVN0921 [H] COG1010 Precorrin-3B methylase Tvo Thermoplasma volc... 184 5e-46 lin1162 [H] COG1010 Precorrin-3B methylase Lin Listeria innocua 181 4e-45 STM2027 [H] COG1010 Precorrin-3B methylase Sty Salmonella typhim... 171 5e-42 >RSp0618_1 [H] COG1010 Precorrin-3B methylase Rso Ralstonia solanacearum Length = 243 Score = 340 bits (872), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 171/243 (70%), Positives = 193/243 (79%) Query: 1 MSGVLNLVSVGPGFGDLIVPRAIAALRDSNVIVAYELYLRWISSHIEGKEIHTPPLTQER 60 MSG L LVSVGPG +LI P AL ++VIVAY+LYL WI+ I K+I T PLTQER Sbjct: 1 MSGKLYLVSVGPGTRELIPPMVKTALDAADVIVAYDLYLTWIAPWITAKDIRTLPLTQER 60 Query: 61 ERALLAIDKARSGEKVALISSGDIGIYAMAALAFDEMKEDDDYQVNVIPGVTSANACASL 120 RA LAI +AR+G VALISSGDIGIYAMAALAF+ M E D + V VIPG+TSANACASL Sbjct: 61 GRAELAIAEARAGRTVALISSGDIGIYAMAALAFEAMAEHDTFDVQVIPGITSANACASL 120 Query: 121 LGSPLSHDFATLSLSNLLCPWEWIENRARHIAQADLACVLYNVQSAGRQEGVYRILKLML 180 LGSPLSHDFATLSLS+LLCPW WIE RARHIAQADLA VLYNVQS RQEGVYRIL ++L Sbjct: 121 LGSPLSHDFATLSLSDLLCPWAWIETRARHIAQADLAVVLYNVQSRQRQEGVYRILDILL 180 Query: 181 ESKAPHTLCGVVKNAYRPGQEVSIHRLDELPSLQFDMLTSIVIGNRFTQRKRGYIFTPRG 240 E K TLCG+V NAYR GQ I L EL + +FDMLTS+VIGNRFT+RKRG+I+TPRG Sbjct: 181 EHKRAETLCGIVHNAYRDGQAAEIVTLAELRTRRFDMLTSLVIGNRFTRRKRGWIYTPRG 240 Query: 241 YND 243 Y + Sbjct: 241 YGN 243 >RSp0618_2 [H] COG2099 Precorrin-6x reductase Rso Ralstonia solanacearum Length = 276 Score = 297 bits (760), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 163/270 (60%), Positives = 191/270 (70%), Gaps = 7/270 (2%) Query: 244 WHD--ESVVAAEEPALPSQAVWVFAGTSDGNALAKQLAAQGHQVVVSTATEYGGELALQD 301 W D ++V AA LP+ AVWVF+GTSDGNALA+ +AA G VVVS+A++YG LA Q+ Sbjct: 1 WDDTPDTVTAA---GLPAHAVWVFSGTSDGNALARDIAATGRAVVVSSASDYGAALAGQN 57 Query: 302 CPGVTVWAGRQGVEARKQALLRHEARVLVDATHPYATLMSQQLMGLSQSLGVPYLRYERP 361 CPGV V AGR G+E R++ L A +VDATHPYA MSQQL+ L+Q L +PYLRYERP Sbjct: 58 CPGVAVVAGRLGIERRRELLRESAAHAIVDATHPYAAEMSQQLIRLAQELALPYLRYERP 117 Query: 362 SAIADIANHALVCDSMEQAAEQAIARGERIFLATGSKDLATFLQAPGADRKQWFVRVTAE 421 A A SME AA A+ G RIFLATGSKDLATF+ A A R WFVRV + Sbjct: 118 GDAQ--AAPAQRAASMEDAARLAMQHGRRIFLATGSKDLATFVNADAAGRHAWFVRVAPD 175 Query: 422 PEFIQRAIDLGIPRSRICAMQGPFSQGFNEALWRDWNIDCVVTKDSGDAGGYRAKADAAK 481 P +QRAID GIPR+R+ AMQGPFSQ NEALWRDW IDCVVTKDSG+AGGY AKA AA Sbjct: 176 PAHLQRAIDAGIPRARLVAMQGPFSQAANEALWRDWGIDCVVTKDSGEAGGYHAKAAAAA 235 Query: 482 ALNIPLLTVRRPALDYPVQAGNLDEALAQV 511 AL IPL+ V RPA+DYP A + L Q+ Sbjct: 236 ALGIPLIVVDRPAIDYPAVAHDFAGVLMQL 265 >TVN0921 [H] COG1010 Precorrin-3B methylase Tvo Thermoplasma volcanium Length = 251 Score = 184 bits (467), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 98/248 (39%), Positives = 150/248 (60%), Gaps = 2/248 (0%) Query: 1 MSGVLNLVSVGPGFGDLIVPRAIAALRDSNVIVAYELYLRWISSHIEGKEIHTPPLTQER 60 M L +V +GPG DL+ P+A A+ S ++ + LY+ +I K + + E Sbjct: 1 MVSSLFVVGIGPGSNDLMTPQASLAIERSEYVIGFSLYVSFIKEKFPNKIYISNGMQGEI 60 Query: 61 ERALLAIDKARSGEKVALISSGDIGIYAMAALAFDEMK--EDDDYQVNVIPGVTSANACA 118 ERA AI+ ARSG V++ISSGD GIY +A F+ + ED+ +V+V+PG+++ ++CA Sbjct: 61 ERAKKAIELARSGHNVSIISSGDAGIYGIAGAVFEILSTLEDNMPEVSVVPGISALSSCA 120 Query: 119 SLLGSPLSHDFATLSLSNLLCPWEWIENRARHIAQADLACVLYNVQSAGRQEGVYRILKL 178 SLLGSPLS+DF LSLS+LL PWE I RA A DL V+YN + + + ++++ Sbjct: 121 SLLGSPLSNDFMVLSLSDLLTPWEVIRKRAEAAAILDLVTVIYNPRGSRFPNNLEKVMEY 180 Query: 179 MLESKAPHTLCGVVKNAYRPGQEVSIHRLDELPSLQFDMLTSIVIGNRFTQRKRGYIFTP 238 L+ ++ T G+V+NAYRP Q I +L + + DMLT+++IGN T ++ T Sbjct: 181 FLKYRSGDTPVGIVRNAYRPDQSAKIVQLKDFSCSEIDMLTTVIIGNSETYVSGRWMATR 240 Query: 239 RGYNDWHD 246 RGY+ +D Sbjct: 241 RGYSGKYD 248 >lin1162 [H] COG1010 Precorrin-3B methylase Lin Listeria innocua Length = 241 Score = 181 bits (459), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 90/240 (37%), Positives = 146/240 (60%), Gaps = 1/240 (0%) Query: 4 VLNLVSVGPGFGDLIVPRAIAALRDSNVIVAYELYLRWISSHIEGKEIHTPPLTQERERA 63 ++ ++ +GPG L+ A+ A+ D++VIV Y Y++ I I+ KE+ + +E +R Sbjct: 1 MIYVIGIGPGDKRLMTGEALEAIADADVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRC 60 Query: 64 LLAIDKARSGEKVALISSGDIGIYAMAALAFD-EMKEDDDYQVNVIPGVTSANACASLLG 122 A++ A +G+KVA++SSGD GIY MA L + K + + +V VIPG+T++ A++LG Sbjct: 61 QEAVNIALTGKKVAVVSSGDAGIYGMAGLVLELAEKSNPELEVKVIPGITASIGAAAVLG 120 Query: 123 SPLSHDFATLSLSNLLCPWEWIENRARHIAQADLACVLYNVQSAGRQEGVYRILKLMLES 182 +P+ HDF +SLS+L+ PWE IE R H A AD YN +S GR + + M+E Sbjct: 121 APIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYNPRSKGRANHLANAFQKMMEH 180 Query: 183 KAPHTLCGVVKNAYRPGQEVSIHRLDELPSLQFDMLTSIVIGNRFTQRKRGYIFTPRGYN 242 K+P T+ G+VK+ R + I ++++ DM T +++GN+ T K G + TPRGY+ Sbjct: 181 KSPKTVVGIVKDVGRKEERKIITTMEDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYS 240 >STM2027 [H] COG1010 Precorrin-3B methylase Sty Salmonella typhimurium LT2 Length = 241 Score = 171 bits (432), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 92/240 (38%), Positives = 142/240 (59%), Gaps = 1/240 (0%) Query: 4 VLNLVSVGPGFGDLIVPRAIAALRDSNVIVAYELYLRWISSHIEGKEIHTPPLTQERERA 63 +L+++ +GPG ++ AI AL+ + ++V Y+ Y + + K++ + +E ER Sbjct: 1 MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERC 60 Query: 64 LLAIDKARSGEKVALISSGDIGIYAMAALAFDEM-KEDDDYQVNVIPGVTSANACASLLG 122 AI+ A++G VALISSGD GIY MA L + + K+ D +V +IPG+T++ A ASLLG Sbjct: 61 QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLG 120 Query: 123 SPLSHDFATLSLSNLLCPWEWIENRARHIAQADLACVLYNVQSAGRQEGVYRILKLMLES 182 +PL HDF +SLS+LL PW IE R +AD YN +S GR+ + R L+ S Sbjct: 121 APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS 180 Query: 183 KAPHTLCGVVKNAYRPGQEVSIHRLDELPSLQFDMLTSIVIGNRFTQRKRGYIFTPRGYN 242 K+ T GVVK+A R +E + L ++ DM + +++GN+ T + G + TPRGY Sbjct: 181 KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT 240