BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0032.p (242 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0624 [H] COG2241 Precorrin-6B methylase 1 Rso Ralstonia solan... 402 e-112 VNG1568G [H] COG2241 Precorrin-6B methylase 1 Hbs Halobacterium ... 127 2e-29 TVN0916_2 [H] COG2241 Precorrin-6B methylase 1 Tvo Thermoplasma ... 109 7e-24 Ta0660_2 [H] COG2241 Precorrin-6B methylase 1 Tac Thermoplasma a... 104 2e-22 SSO2296 [H] COG2241 Precorrin-6B methylase 1 Sso Sulfolobus solf... 93 5e-19 >RSp0624 [H] COG2241 Precorrin-6B methylase 1 Rso Ralstonia solanacearum Length = 235 Score = 402 bits (1033), Expect = e-112, Method: Compositional matrix adjust. Identities = 194/232 (83%), Positives = 207/232 (89%) Query: 1 MIICIGAGPGDIGYLTQRGAELIRDADVVAGFDAVVNVVQSIIPPTAEVILMNYRDQVAQ 60 MIICIGAGPGDIGYL QR +LIR ADVVAGF+AVV+VV+ +IP TA+VI M YRDQVAQ Sbjct: 1 MIICIGAGPGDIGYLPQRSVDLIRHADVVAGFNAVVDVVRPLIPDTAQVIQMGYRDQVAQ 60 Query: 61 LDLVAAAHHAGKRCVVVFMGDIHFSGFQYLERVERACGHPVETLPGISSAQILASRAKVC 120 LD VA HHAG+RCVVVFMGDIHFSGFQYLERVERACGHPVET+PGISSAQILASRAKVC Sbjct: 61 LDEVARLHHAGQRCVVVFMGDIHFSGFQYLERVERACGHPVETVPGISSAQILASRAKVC 120 Query: 121 FDETTFITFHRRGDLTPFKRHLVHVLNDGRNAIVIPCPWDAARSFMPRHIAAYLLENGVQ 180 FDET+FITFHRRGDLTPFKRHLVHVL D RNAIVIPCPWDAARSFMP HIAAYLLENG+ Sbjct: 121 FDETSFITFHRRGDLTPFKRHLVHVLQDQRNAIVIPCPWDAARSFMPWHIAAYLLENGIP 180 Query: 181 PDHPTEVWENLTRGDAEWHGSLAECVDKEFTDMSIMLIRTPTPMDSQIEPPP 232 P H EVWE LTR +AEWHGSLAEC D+ F+DMSIMLIRT PM SQIEPPP Sbjct: 181 PGHRVEVWERLTRSEAEWHGSLAECADQRFSDMSIMLIRTLHPMASQIEPPP 232 >VNG1568G [H] COG2241 Precorrin-6B methylase 1 Hbs Halobacterium sp. NRC-1 Length = 250 Score = 127 bits (320), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 85/223 (38%), Positives = 120/223 (53%), Gaps = 14/223 (6%) Query: 4 CIGAGPGDIGYLTQRGAELIRDADVVAGFDAVVNVVQSIIPPTAEVILMNYRDQVAQLDL 63 +G GPG+ +LT RG IRDA+V+ GF+ VV V + A+++ YRD+ A L Sbjct: 32 AVGIGPGNPAFLTARGERAIRDAEVIVGFETVVEFVADLT--DADLLTCGYRDEQAALSA 89 Query: 64 VAAAHHAGKRCVVVFMGDIHFSGFQYLERVERACGHPVETLPGISSAQILASRAKVCFDE 123 G R V MGD + SG+Q+L +VERA G PV +PGISS Q+ ASRA+ ++ Sbjct: 90 FGERVADGARGTAVLMGDPNHSGYQFLGKVERAVGEPVRVVPGISSLQVAASRARTPMED 149 Query: 124 TTFITFHRRGDLTPFKRHLVHVLNDGRNAIVIPCPWDAARSFMPRHI-AAYLLENGVQPD 182 TTF+T H+ GD++ L + D R+ +V+P P+D MP I A L PD Sbjct: 150 TTFVTLHKSGDVSDGVARLRDAVGD-RHLLVLPRPFD----LMPGDIAADLLEAADAAPD 204 Query: 183 HPTEVWENLTRGDAEWH----GSLAECVDKE--FTDMSIMLIR 219 V E LT D G LA +E F+D+S++ +R Sbjct: 205 LDALVLERLTHDDESITRTTLGELAAHAGEETPFSDLSVLAVR 247 >TVN0916_2 [H] COG2241 Precorrin-6B methylase 1 Tvo Thermoplasma volcanium Length = 218 Score = 109 bits (272), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 68/215 (31%), Positives = 112/215 (52%), Gaps = 7/215 (3%) Query: 5 IGAGPGDIGYLTQRGAELIRDADVVAGFDAVVNVVQSIIPPTAEVILMNYRDQVAQLDLV 64 IG GP D +T + I AD+VAG++ + + ++ I +++L +++DQ ++ + Sbjct: 4 IGVGPSDPSLMTLAAIDRIAKADIVAGYEMPMRIARNAIGKKEQIVL-HWKDQQKYVERI 62 Query: 65 AAAHHAGKRCVVVFMGDIHFSGFQYLERVERACGHPVETLPGISSAQILASRAKVCFDET 124 + G R +F GD FS + + R C + E +PGISS Q ++R+ + + T Sbjct: 63 IDLYDKGYRIAFLFTGDSCFSESELIRRFIGRCRN-YEIIPGISSVQAASARSGMPLELT 121 Query: 125 TFITFHRRGDLTPFKRHLVHVLNDGRNAIVIPCPWDAARSFMPRHIAAYLLENGVQPDHP 184 I+FH G + K+ ++ L +VIP P+D FMP IA YL+ENG+ Sbjct: 122 PVISFHVTGYIEDRKKEMLDSLRRNGRVLVIPRPYD----FMPSKIADYLIENGISGSTE 177 Query: 185 TEVWENLTRGDAE-WHGSLAECVDKEFTDMSIMLI 218 V E LT + H +L + K+F+D+SIM I Sbjct: 178 VTVMEYLTSENERVTHNTLEQIKHKDFSDISIMAI 212 >Ta0660_2 [H] COG2241 Precorrin-6B methylase 1 Tac Thermoplasma acidophilum Length = 217 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 66/221 (29%), Positives = 114/221 (51%), Gaps = 8/221 (3%) Query: 2 IICIGAGPGDIGYLTQRGAELIRDADVVAGFDAVVNVVQSIIPPTAEVILMNYRDQVAQL 61 I IG GP D +T + I +D+VAG+ +++ +S++ +V+ ++++Q + Sbjct: 1 ISFIGVGPSDPDLMTMKARRAIVASDIVAGYQMPLDIAKSMLWNKPKVVF-HWKEQQRYV 59 Query: 62 DLVAAAHHAGKRCVVVFMGDIHFSGFQYLERVERACGHPVETLPGISSAQILASRAKVCF 121 D V + G R +F GD F+ + + R C + + +PGISS Q +S + Sbjct: 60 DEVIDLYEKGYRIAYLFTGDSCFTESELIRRFTSKCRN-FDIIPGISSVQAASSITGMAI 118 Query: 122 DETTFITFHRRGDLTPFKRHLVHVLNDGRNAIVIPCPWDAARSFMPRHIAAYLLENGVQP 181 + I+FH GD+ K L+ +++ + AI+IP P+D FMP+ IA +L + G Sbjct: 119 EMAGIISFHVTGDIEDRKADLLRTISERKRAIIIPRPYD----FMPKDIAKFLTDRGFG- 173 Query: 182 DHPTEVWENLTRGDAEWHGS-LAECVDKEFTDMSIMLIRTP 221 D P V E T D S L + + +F+D+SIM++ P Sbjct: 174 DLPAVVIERATAADERRTASFLKDLIGSDFSDISIMVVGKP 214 >SSO2296 [H] COG2241 Precorrin-6B methylase 1 Sso Sulfolobus solfataricus Length = 227 Score = 93.2 bits (230), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 65/224 (29%), Positives = 120/224 (53%), Gaps = 15/224 (6%) Query: 2 IICIGAGPGDIGYLTQRGAELIRDADVVAGFDAVVNVVQSIIPPTAEVILMNYRDQVAQL 61 + +G GPGD YLT +G + IRD+ VVAG+ +V+ I+ V+L Y+++ L Sbjct: 8 VYIVGVGPGDPEYLTLKGYKAIRDSVVVAGWKSVLERFSPILEGKRTVVL-TYKNESETL 66 Query: 62 DLVAAAHHAGKRCVVVFMGDIHFSGFQYLERVER---ACGHPVETLPGISSAQILASRAK 118 + V + ++ GD S +Q++E+++ + G V + G+SS I SR Sbjct: 67 EEVIEIGKT-ENVAILDHGDPSVSDWQFVEKIKNIAASKGVKVNIISGVSSLNIALSRLG 125 Query: 119 VCFDETTFITFHRRGDLTPFKRHLVHVLNDGRNAIVIPCPWDAARSFMPRHIAAYLLENG 178 + + F+T H RGD++ F ++++L GR AIVIP P P+ +A +L +N Sbjct: 126 LDINFIGFVTLHVRGDISKFLNDILNILRMGRVAIVIPEPHKDG----PQKVAKFLYDNN 181 Query: 179 VQPDHPTEVWENLTRGDAEW--HGSLAECVDK--EFTDMSIMLI 218 + + V+E L+ D + + +L +++ +F+D+++M+I Sbjct: 182 L--NCRIVVFEKLSYDDEKRRVYDNLVSLMNEKNDFSDLTMMVI 223