BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0160.p (522 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1597 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia s... 538 e-152 alr4267 [V] COG0841 Cation/multidrug efflux pump Nos Nostoc sp. ... 132 3e-30 SMa0875 [V] COG0841 Cation/multidrug efflux pump Sme Sinorhizobi... 104 6e-22 mll7357 [V] COG0841 Cation/multidrug efflux pump Mlo Mesorhizobi... 101 4e-21 BH3816 [V] COG0841 Cation/multidrug efflux pump Bha Bacillus hal... 101 4e-21 >RSp1597 [V] COG0841 Cation/multidrug efflux pump Rso Ralstonia solanacearum Length = 1064 Score = 538 bits (1386), Expect = e-152, Method: Compositional matrix adjust. Identities = 260/388 (67%), Positives = 310/388 (79%), Gaps = 6/388 (1%) Query: 1 VTIENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLA 60 VTIENIERH+H GK AI+ GA EI VPAFVST+CICIVFVPMFFL+GVAR+LFVP+A Sbjct: 405 VTIENIERHLHEGKAPITAILDGAAEIAVPAFVSTLCICIVFVPMFFLTGVARYLFVPMA 464 Query: 61 EAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRR 120 EAV+FAM ASY+LSRTLVPTLVM+LM G H + G ++ +Y+ F++RFEH+R Sbjct: 465 EAVIFAMLASYVLSRTLVPTLVMMLMTG-HTEDAKKPGP----MRYIYQAFNARFEHMRG 519 Query: 121 AYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIE 180 Y +L LL HRR FAL+FLGFCV SC LY LGRDFFP+VD GQIRLHMR P+G RIE Sbjct: 520 NYAAILGGLLEHRRVFALSFLGFCVASCALYLALGRDFFPDVDAGQIRLHMRAPSGLRIE 579 Query: 181 ETARAADQVEAAIREMIPAHELETILDNIGVPNSGINLSYSNAGTIGTLDGEILMSLHE- 239 ETAR AD+VEAAIR +IP +EL T+LDN+GVPNSGINLSYSNAGTIGTLDGEIL++L E Sbjct: 580 ETARLADEVEAAIRTIIPRNELATLLDNLGVPNSGINLSYSNAGTIGTLDGEILIALREG 639 Query: 240 DHRPTEQLVSMLRAELPKRFPGIEFFFQPADIVTQILNFGLPAAIDVQFTGPNLQQNAEL 299 + +P+ + V LR ELPKRFPG+EF FQPADIV+QILNFGLP+AIDVQF+G ++ N +L Sbjct: 640 ERKPSAEYVRRLREELPKRFPGVEFAFQPADIVSQILNFGLPSAIDVQFSGADVAANQQL 699 Query: 300 AAQLTKQIRQIPGAVDAHVHQRLDGPALDMQMDRTRLQQFGLSAANVGQNVLIALSGSSQ 359 A L +IR+IPGAVD HVHQR D P L + MDRTR+QQ GL +V QN+L++LS S Q Sbjct: 700 AGILANRIRRIPGAVDTHVHQRFDQPTLAVNMDRTRIQQVGLQGRDVAQNLLVSLSSSFQ 759 Query: 360 TTPAFWLNPQNGVVYNIAVQSPQYRTGT 387 T+P FWLNP NGVVY +AVQSPQYR + Sbjct: 760 TSPTFWLNPVNGVVYQVAVQSPQYRVDS 787 Score = 203 bits (517), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 104/129 (80%), Positives = 114/129 (88%) Query: 383 YRTGTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLM 442 + TGTTLSVP+LTGAIMTMGVATANSIL++SFAR+R EG P+ AALE+GATR+RPVLM Sbjct: 931 FVTGTTLSVPSLTGAIMTMGVATANSILLISFARERLHEGIAPMQAALEAGATRLRPVLM 990 Query: 443 TALAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARRK 502 TALAMIIGM+PMALGLGEGAEQNAPLGRAVIGGL+FATVSTLFFVPVVFA VH RL RR Sbjct: 991 TALAMIIGMVPMALGLGEGAEQNAPLGRAVIGGLLFATVSTLFFVPVVFASVHGRLQRRA 1050 Query: 503 EARRQDGRG 511 R D RG Sbjct: 1051 GRRGTDPRG 1059 >alr4267 [V] COG0841 Cation/multidrug efflux pump Nos Nostoc sp. PCC 7120 Length = 844 Score = 132 bits (331), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 146/595 (24%), Positives = 244/595 (41%), Gaps = 130/595 (21%) Query: 3 IENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLAEA 62 +ENI RH+ G+ A EIG+ +T VF+P+ + GV F P Sbjct: 290 VENILRHIEEGETPRQAAHSATDEIGLAVVATTAAAVAVFLPIGLMGGVVGQFFKPFGIT 349 Query: 63 VVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRRAY 122 V + AS +++RTL P L + +TS QR +F R Sbjct: 350 VSASYIASTLVARTLSPVLATYWL---------KPATQTS--QRSEANIWGKFTQFYRN- 397 Query: 123 TLVLSSLLSHRRR-FALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMP------- 174 +L+ L+HR+ LA L F V L PF+ + F P +D G+ + P Sbjct: 398 --LLNWSLNHRKTVITLAVLSF-VAGIALIPFIPKGFIPKLDRGEFNITYTAPLPKIPDL 454 Query: 175 --------TGTR------------------IEETARAADQVEAAIREMIPAHELETILDN 208 G R + ++ AA ++E +R+ + ++ET+ Sbjct: 455 AALQLQAGAGQRSNITQSPIPNPQSPIPNPLNDSLEAAKKLEEVVRK---SPDVETVFTT 511 Query: 209 IGVPNSGINLSYSNAGTIGTLDGEILMSLHEDHR-PTEQLVSMLRAELPKRFPGIEFFFQ 267 +G N G + + L ED + T +L LR+ LP PG+ + Sbjct: 512 VGSREGEPN------------KGTLYVKLKEDRKIQTAELQDQLRSTLPS-LPGVTTSVE 558 Query: 268 PADIVTQILNFGLPAAIDVQFTGPNLQQNAELAAQLTKQIRQIPGAVDAHVHQRLDGPAL 327 DI Q ++ G + V +G +LQ + A ++ ++I +IPG D V + Sbjct: 559 --DI--QFVDTGGQKPLQVALSGNDLQALNQTAKKIKERIEKIPGFADVTVTGETNTQDQ 614 Query: 328 DMQMDRTRLQQFG---------LSAANVGQNVL----------IALS------------G 356 +Q++R Q+ LS N ++ I L+ G Sbjct: 615 VLQIERLNNQRVAYISANLGQDLSLGNATDKIVAEAQPILPAGITLNLGGDSARQSEVFG 674 Query: 357 SSQTTPAF---------------WLNPQNGVVYNIAVQSPQYRTGTTLSV---------P 392 S +T A W++P I V P G L++ Sbjct: 675 SFASTLALSALCIIVVLVLLFKSWIDP-----IVIGVSLPLSIVGAMLALLFTKSDFGMI 729 Query: 393 ALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTALAMIIGMI 452 +L G + +G+A N+I++V + Q R+ G A L++G R+RP++MT ++ ++GM+ Sbjct: 730 SLIGFVFLLGLANKNAIVLVDYINQLRQAGLNRTEAILKAGPVRLRPIIMTTISTLLGML 789 Query: 453 PMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARRKEARRQ 507 P+ALGLG G+E +P+ A+ GGL+ +T+ +L +PVV+A + R R Sbjct: 790 PIALGLGAGSELRSPMAVAIAGGLVTSTILSLIVIPVVYAILDDWFPRFAHKERN 844 >SMa0875 [V] COG0841 Cation/multidrug efflux pump Sme Sinorhizobium meliloti Length = 1065 Score = 104 bits (259), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 52/107 (48%), Positives = 76/107 (71%) Query: 385 TGTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTA 444 TG+TL++ ++ G +M MG+ T N+IL+V ++ EG + + ++GA R+RP++MT Sbjct: 909 TGSTLNMFSMIGIMMLMGLVTKNAILLVDYSNLGVREGKSLRQSLADAGAVRLRPIVMTT 968 Query: 445 LAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVF 491 LAMI GM+P ALGLGEG Q AP+ A+IGGLI +T+ +L FVPVV Sbjct: 969 LAMIFGMLPTALGLGEGGAQRAPMAHAIIGGLISSTLLSLVFVPVVL 1015 Score = 101 bits (251), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 93/381 (24%), Positives = 159/381 (41%), Gaps = 32/381 (8%) Query: 4 ENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLAEAV 63 ENI RH+ MGK A + G EIG+ +T+CI VF+P+ F+ G+ F+ V Sbjct: 408 ENITRHLQMGKDPVRAALDGTNEIGLAVLSTTLCIVAVFLPVAFMGGLIGRFFLQFGVTV 467 Query: 64 VFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRRAYT 123 A+ S +S TL P ++ V K +L FD FE + Y Sbjct: 468 AVAVVISLFVSFTLDP-----MLSSVWCDPQSQKTAKRGFFGQLIERFDQWFEGLASRYR 522 Query: 124 LVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIEETA 183 V+ +R+ LG V+S LL P +G +F P D G++ + + G ++ A Sbjct: 523 SVIYFTFDYRKTTIAIVLGMFVVSLLLVPRIGTEFLPPPDQGEVSISLEANEGASLDYMA 582 Query: 184 RAADQVEAAIREMIPAHELETILDNIGVPNSGINLSYSNAGTIGTLDGEILMSLHEDHR- 242 Q+E A+R E + NSG ++ A L +H R Sbjct: 583 AKVGQIERALR------EFNYVSSTYSTINSGEMRGFNKALVAVQL-------VHSSQRR 629 Query: 243 -PTEQLVSMLRAELPKRFPGIEFFF-QPADIVTQILNFGLPAAIDVQFTGPNLQQNAELA 300 T + + +R L R G+E Q +++V I + + G ++ ++ Sbjct: 630 LKTAETLGPIRRRL-SRIAGLEISVGQRSEVVGSI------KPLQLSILGDGDEELRRIS 682 Query: 301 AQLTKQIRQIPGAVDAHVHQRLDGPALDMQMDRTRLQQFGLSAANVGQNVLIALSGSSQT 360 +T + IPGA + P L +++ R G+S A +G + ++G + Sbjct: 683 DHITSVLAAIPGATEIESSIEKLRPTLAVRVRREAASDLGVSIATIGDTLRSLVAGDA-- 740 Query: 361 TPAFWLNPQNGVVYNIAVQSP 381 + W +P +G +++ V+ P Sbjct: 741 -ISVWNSP-DGETHDVVVRLP 759 >mll7357 [V] COG0841 Cation/multidrug efflux pump Mlo Mesorhizobium loti Length = 1061 Score = 101 bits (252), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 51/116 (43%), Positives = 75/116 (64%) Query: 386 GTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTAL 445 G + V + G +M +G+A N+ILIV FAR+RREEG + + AA+E+ R+RP+LMTA Sbjct: 920 GMQIDVYSQIGFVMLIGLAAKNAILIVEFARRRREEGLSIVEAAMEAARLRLRPILMTAF 979 Query: 446 AMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARR 501 A I+G++P+ G GA +G V GG++ AT+ +L FVPV +A + Q RR Sbjct: 980 AFILGVLPLMFATGAGAASRQSIGTTVFGGMVAATILSLVFVPVFYAVIEQLRERR 1035 Score = 67.4 bits (163), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 71/274 (25%), Positives = 115/274 (41%), Gaps = 23/274 (8%) Query: 1 VTIENIERHMHMG-KPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPL 59 V +EN+ER + G KPL A E+ P +T + VFVP+ F+ GV+ L+ Sbjct: 416 VVVENVERQLEAGLKPLA-ATKAAMAEVTGPIIATTAVLMAVFVPVAFIPGVSGRLYNQF 474 Query: 60 AEAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVR 119 A V ++ S S TL P L + HG + L+R F++ F+ + Sbjct: 475 ALTVAISVGISAFNSLTLSPALSAAFLRHRHGPQF-----------FLFRWFNTGFDKIS 523 Query: 120 RAYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRI 179 AY + L+ R F V + ++ L F P D G + +++P G + Sbjct: 524 HAYAHGVRFLIRLRWIMLGLFAAGLVATYFVWQKLPSTFLPVEDQGYFFVVIQLPDGASL 583 Query: 180 EETARAADQVEAAIREMIPAHELETILDNIGVPNSGINLSYSNAGTIGTLDGEILMSLHE 239 E T D V R+++ A I+ +I SG+N S A + ++ IL E Sbjct: 584 ERT----DAVAQKARDILQATPGVDIVGSI----SGLNFLTSAAQSNSAVEFAILKPWEE 635 Query: 240 D--HRPTEQLVSMLRAELPKRFPGIEFFFQPADI 271 + +LV+ +R +L + F P I Sbjct: 636 RGPDQSASKLVADVRGKLMQIPEAFALSFDPPSI 669 >BH3816 [V] COG0841 Cation/multidrug efflux pump Bha Bacillus halodurans Length = 1093 Score = 101 bits (252), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 54/117 (46%), Positives = 74/117 (63%), Gaps = 1/117 (0%) Query: 385 TGTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTA 444 TGT+ SV A G I+ G+ N+I+IV + Q + GAT A +E+ R RPVLMTA Sbjct: 896 TGTSFSVMAGIGMIILAGIVVNNAIVIVDYMNQLKASGATSYEAIVEAVKQRTRPVLMTA 955 Query: 445 LAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARR 501 L I+G++P+AL LGEG E P+G VIGGLI T TLF +PV+++ + + RR Sbjct: 956 LTTILGVLPLALALGEGTEMQRPIGLTVIGGLISGTFLTLFVIPVIYS-LFDKDTRR 1011 Score = 68.6 bits (166), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 55/203 (27%), Positives = 88/203 (43%), Gaps = 32/203 (15%) Query: 1 VTIENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLA 60 V IENI RH+ MGK A + G E+ ST+ VFVP+ FL+G+ LF+ A Sbjct: 402 VVIENIYRHLSMGKEPKQAALEGTREVRGAITASTLTTISVFVPILFLTGLVGELFIQFA 461 Query: 61 EAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRR 120 V ++ AS +++ TLVP LM RL R F E R+ Sbjct: 462 LTVSLSLFASLLVAITLVP-----LMAS-----------------RLLRKFQQNEEAKRQ 499 Query: 121 AYTLVLSSLLSHRRRFALAFLGFCVLSCLL--------YPFLGRDFFPNVDGGQIRLHMR 172 +L+ + ++AL +++ LL +G DF ++D G + + + Sbjct: 500 QSRFILT--IERSIKWALRHRAMVLIATLLLFVGGLYGLSTVGVDFLEDMDEGLMVVEIE 557 Query: 173 MPTGTRIEETARAADQVEAAIRE 195 GT +E T + +E + + Sbjct: 558 HEPGTSLERTRETVEAIEQKLED 580