BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0127.p (530 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4900 [GEPR] COG0477 Permeases of the major facilitator superfa... 220 7e-57 RSc1093 [GEPR] COG0477 Permeases of the major facilitator superf... 158 2e-38 AGc4589 [GEPR] COG0477 Permeases of the major facilitator superf... 148 2e-35 PA0235 [GEPR] COG0477 Permeases of the major facilitator superfa... 143 8e-34 Cgl2967 [GEPR] COG0477 Permeases of the major facilitator superf... 137 7e-32 >PA4900 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 446 Score = 220 bits (560), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 130/328 (39%), Positives = 184/328 (56%), Gaps = 16/328 (4%) Query: 1 MQQIDVIKLANEAKFNKFHRLILFWGVLILVLDGYDLAVVGAALPSIMKEMGVDASSAGF 60 M + + + A+FN+ H LIL WG I++ DGYD+ + G+ +P +M+E + AG Sbjct: 1 MNRQSAAAVIDAARFNRTHWLILGWGCFIMLFDGYDMVIYGSVVPRLMQEWQLSPVQAGT 60 Query: 61 MASSALFGMMFGAMFFGTLADKIGRPLMLAVGVGTFSVFTAAAGLAHDPISFSALRFLAG 120 + S ALFGM+FG LAD+ GR ++ S+ G A DP+ A RF G Sbjct: 61 LGSCALFGMLFGGTLLAPLADRFGRRRLVIATTLLASLAAFLTGHARDPLELGAGRFFTG 120 Query: 121 LGIGGVLPVVTAQMGEFSPVKVRARLVTLVFAGYSVGGVLVALTGKQLIESYGWQSVFFV 180 L +G ++P + EF+P R+ LVT++ A YSVG VL AL +I ++GWQSVF+V Sbjct: 121 LALGALVPSAINLISEFAPAGRRSTLVTVMSAFYSVGAVLSALLAIAMIPAWGWQSVFYV 180 Query: 181 AGLPVLLIPFILKTMPESMATLHRKGRQARLAEIALKINPALRLNGNEEFILPVAER--- 237 A LPVL +P +L+ +PES A L KGR+A L + K++P R P AER Sbjct: 181 AVLPVLAVPLMLRWLPESAAFLELKGRRAELDALLRKVDPDYR---------PGAERANA 231 Query: 238 -VANAP---VKMLFANGRGFSTLMIWVAYFTGLFMVYSLSSWLTKLMAMAGYSLGSALNF 293 A AP V LF + TL++WVA+ + M Y L++WL KLMA GY+LGS+L F Sbjct: 232 VAAEAPSGRVAQLFEGRQAVGTLLLWVAFAMCMLMSYGLNTWLPKLMAGGGYALGSSLAF 291 Query: 294 VLVFNFGAMAGAQPLQEVEDNLASLRIL 321 ++ N GA GA + D L + R L Sbjct: 292 LVTLNVGATLGALFGGWLADRLGAGRTL 319 Score = 107 bits (267), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 62/125 (49%), Positives = 85/125 (68%) Query: 387 ALGGGWLGDKFNIKHVLVAFYVVGAISLTLMGFTKETWLLFAVVFVVGASTLGTQLLAYA 446 AL GGWL D+ LV F+ + A SL +G WLL ++ V GA+T+GT + +A Sbjct: 303 ALFGGWLADRLGAGRTLVLFFALAAASLAALGLGPGPWLLNGLLVVAGATTIGTLAVIHA 362 Query: 447 YAGEFYPVAIRSTGVGFASGVGRLGAIIAPVLIGALVTLKLPLEQNFLGIAVAGLIGAVA 506 YA +FYP +RSTGVG+A+GVGRLGAI P+L G+L+ L+LP +QNFL A G+IGA+A Sbjct: 363 YAAQFYPAWVRSTGVGWAAGVGRLGAIAGPMLGGSLLALELPFQQNFLAFAAPGVIGALA 422 Query: 507 VMLIN 511 + I+ Sbjct: 423 IACIH 427 >RSc1093 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 454 Score = 158 bits (400), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 99/322 (30%), Positives = 170/322 (52%), Gaps = 5/322 (1%) Query: 4 IDVIKLANEAKFNKFHRLILFWGVLILVLDGYDLAVVGAALPSIMKEMGVDASSAGFMAS 63 IDV + +F+ F ++ ++ +DG+D A VG P++ ++ + + G + S Sbjct: 8 IDVRAWLDRQRFSAFQWSVVLLCFFVVAIDGFDTACVGFIAPALAQDWHIGPAVLGTVFS 67 Query: 64 SALFGMMFGAMFFGTLADKIGRPLMLAVGVGTFSVFTAAAGLAHDPISFSALRFLAGLGI 123 + L G+M GA+ FG LAD+IGR L VG F + + + A + ALRFL GLG+ Sbjct: 68 AGLAGLMVGALIFGPLADRIGRKQTLLFTVGAFGLASVLSAFAPSVGALVALRFLTGLGL 127 Query: 124 GGVLPVVTAQMGEFSPVKVRARLVTLVFAGYSVGGVLVALTGKQLIESYGWQSVFFVAG- 182 GG +P A E+ P + RA L T++F G+++G + QL+ +GW+SV G Sbjct: 128 GGAMPNAIALTSEYCPERRRAFLTTVMFCGFTLGSGFGGIVAAQLVPEFGWRSVLLFGGV 187 Query: 183 LPVLLIPFILKTMPESMATLHRKGRQ-ARLAEIALKINPALRLNGNEEFIL--PVAERVA 239 +P+LL+P ++ +PES+ L KG Q A + + +I P +++ + F+L A+ Sbjct: 188 IPLLLLPVMVFALPESVRYLVAKGGQSAAVGRLLNRIAP-VQVTPDTRFVLHETAAKGAP 246 Query: 240 NAPVKMLFANGRGFSTLMIWVAYFTGLFMVYSLSSWLTKLMAMAGYSLGSALNFVLVFNF 299 +PV+ LF TL++W +F L +VY +++WL L+ G +L A +++ Sbjct: 247 GSPVRQLFLPAFRTGTLLLWSVFFMSLLIVYLMTNWLPTLIHSGGVALALASRIAVMYQL 306 Query: 300 GAMAGAQPLQEVEDNLASLRIL 321 G GA + + D + +L Sbjct: 307 GGTVGALVIGRMMDRYPATVVL 328 Score = 61.6 bits (148), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 42/117 (35%), Positives = 65/117 (55%), Gaps = 1/117 (0%) Query: 395 DKFNIKHVLVAFYVVGAISLTLMGFTKETWLLFAVVFVVGASTLGTQLLAYAYAGEFYPV 454 D++ VL Y +GA L L G ++ T L AV V G G+Q+ A A+A +FYP Sbjct: 320 DRYPATVVLCCTYGLGAGFLVLTGVSQGTMLAIAVTGV-GFCISGSQIGANAFAAQFYPT 378 Query: 455 AIRSTGVGFASGVGRLGAIIAPVLIGALVTLKLPLEQNFLGIAVAGLIGAVAVMLIN 511 A R TG+ +A G+GRLG++ ++ G L+ + + FL +AV I ++A+ L Sbjct: 379 ASRVTGISWALGIGRLGSVCGALIGGVLLAANIGFQLLFLLVAVPAAIASLAIFLCG 435 >AGc4589 [GEPR] COG0477 Permeases of the major facilitator superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 477 Score = 148 bits (374), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 104/279 (37%), Positives = 162/279 (58%), Gaps = 11/279 (3%) Query: 28 LILVLDGYDLAVVGAALPSIMKE--MGVDASSAGFMASSALFGMMFGAMFFGTLADKIGR 85 L+++ DGYDL V GA P+++KE ++ + G A+ L GM+ GA+F GT+AD+IGR Sbjct: 74 LMIMFDGYDLVVYGAVAPALLKESTWALNPAMVGRAAALTLVGMLLGALFAGTMADRIGR 133 Query: 86 PLMLAVGVGTFSVFTAAAGLAHDPISFSALRFLAGLGIGGVLPVVTAQMGEFSPVKVRAR 145 ++ + + FS A+ + + ++F RF AGLG+G +LP VTA + EFSP + RA+ Sbjct: 134 RKVVLLSLAGFSAMMIASAMTPNFLAFEITRFFAGLGLGALLPTVTALVLEFSPPQRRAQ 193 Query: 146 LVTLVFAGYSVGGVLVALTGKQLIESYGWQSVFFVAGLPVLLIPFILKTMPESMATLHRK 205 +L F GY +GG++ + G L+ESYGW+ + + GLP++L+P ++ +PES L K Sbjct: 194 ANSLSFLGYLIGGIISGILGMLLLESYGWRPLMLIGGLPLILLPIFMRFLPESPEWLASK 253 Query: 206 GRQARLAEIALKINPALRLNGNEEFILPVAERVANAPVKMLFANGRGFSTLMIWVAYFTG 265 GRQA I + I+P A ++ V LF+ GR STL W +F Sbjct: 254 GRQAEADGICDSY--------GLQRIVPHA-KMQKGGVGALFSEGRLASTLNAWGIHFCS 304 Query: 266 LFMVYSLSSWLTKLMAMAGYSLGSALNFVLVFNFGAMAG 304 L + + + +WL +M GY + SAL+F ++ N GA G Sbjct: 305 LLLTFGMVNWLPTIMNKMGYDISSALSFSVMLNVGAAIG 343 Score = 75.9 bits (185), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 51/123 (41%), Positives = 69/123 (56%), Gaps = 4/123 (3%) Query: 390 GGWLGDKFNIKHVLVAFYVVGAISLTLMGFTKETWLLFAVVFVVGASTLGTQLLAYAYAG 449 GG DK N+K V+ + VGA S+ + K LL+ V + GA T+GTQ+LA G Sbjct: 347 GGRFADKGNVKVVVAILFAVGAASIFALTANKGP-LLYVFVALAGAGTIGTQILANVLVG 405 Query: 450 EFYPVAIRSTGVGFASGVGRLGAIIAPVLIGALVTLKLPLEQNFL---GIAVAGLIGAVA 506 YPV IR TG+GF+ VGRLG I P++ G ++ L E NF +AV GL+ V Sbjct: 406 RLYPVHIRGTGLGFSLAVGRLGGIAGPMIGGLVLQRGLAPEWNFYIFGSVAVVGLMLTVL 465 Query: 507 VML 509 +L Sbjct: 466 TLL 468 >PA0235 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 448 Score = 143 bits (361), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 104/325 (32%), Positives = 173/325 (53%), Gaps = 4/325 (1%) Query: 1 MQQIDVIKLANEAKFNKFHRLILFWGVLILVLDGYDLAVVGAALPSIMKEMGVDASSAGF 60 ++++DV N + + I+ LI+ LDG D A +G P++ ++ G+D +S G Sbjct: 9 VERLDVQAFINAQPLSPYQWRIVLLCFLIVFLDGLDTAAMGFIAPALTQDWGIDRASLGP 68 Query: 61 MASSALFGMMFGAMFFGTLADKIGRPLMLAVGVGTFSVFTAAAGLAHDPISFSALRFLAG 120 + S+AL GM+FGA+ G LAD+ GR L+L V F +F+ A+ + + ALRFL G Sbjct: 69 VMSAALIGMVFGALGSGPLADRYGRKLVLVAAVFLFGLFSLASAYSTNVEQLLALRFLTG 128 Query: 121 LGIGGVLPVVTAQMGEFSPVKVRARLVTLVFAGYSVGGVLVALTGKQLIESYGWQS-VFF 179 LG+G +P T + E++P ++++ LVT +F G+++G +LI +GW S + Sbjct: 129 LGLGAAMPNATTLLSEYTPERLKSLLVTSMFCGFNLGMACGGFVSAKLIPLFGWHSLLLL 188 Query: 180 VAGLPVLLIPFILKTMPESMATLHRKGR-QARLAEIALKINPALRLNGNEEFILPVAERV 238 LP++L +L +PES L + R R+ ++ I PA ++ F +P + V Sbjct: 189 GGLLPLVLAVVLLFRLPESARYLVVRNRGSERVRQVLAPIAPA-QVALARSFHVPEQQTV 247 Query: 239 -ANAPVKMLFANGRGFSTLMIWVAYFTGLFMVYSLSSWLTKLMAMAGYSLGSALNFVLVF 297 A ++F+ TL++W+ YF GL +VY L+SWL LM +G SL A +F Sbjct: 248 QARNVFAVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASLEQAAFIGALF 307 Query: 298 NFGAMAGAQPLQEVEDNLASLRILG 322 FG + A + D +++G Sbjct: 308 QFGGVLSAVAVGWAMDRFNPHKVIG 332 >Cgl2967 [GEPR] COG0477 Permeases of the major facilitator superfamily Cgl Corynebacterium glutamicum Length = 444 Score = 137 bits (345), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 91/298 (30%), Positives = 149/298 (50%), Gaps = 30/298 (10%) Query: 26 GVLILVLDGYDLAVVGAALPSIMKEMGVDASSAGFMASSALFGMMFGAMFFGTLADKIGR 85 G ++ DGYDL V G ++ KE + +++ G + S+A FGM GA+F G L+D++GR Sbjct: 47 GWFFVIFDGYDLIVYGTVQSALAKEWNLSSATLGTIGSTAFFGMAIGAVFIGRLSDRVGR 106 Query: 86 PLMLAVGVGTFSVFTAAAGLAHDPISFSALRFLAGLGIGGVLPVVTAQMGEFSPVKVRAR 145 + V SVFT A +P F A RF+AGLG+GG++P V A + P K + Sbjct: 107 KAAVIGSVLILSVFTMLCAFAPNPWVFGAFRFIAGLGLGGLVPSVNAMTSDLVPRKTMSA 166 Query: 146 LVTLVFAGYSVGGVLVALTGKQLI---ESYGWQSVFFVAGLPVLL-IPFILKTMPESMAT 201 T++ +G +GG + A+ ++ E +GW+ +F +A +P+++ +P +K +P Sbjct: 167 WATVMMSGVPIGGSIAAVLALVVVPSSEEWGWRFMFLIALIPLVVGLPIAMKVIP----- 221 Query: 202 LHRKGRQARLAEIALKINPALRLNGNEEFILPVAERVANAPVKMLFANGRGFSTLMIWVA 261 ++ A+K + +R G++E A K L + + ++ +A Sbjct: 222 ----------SDKAIKADHDIR-EGHDE----------PAGFKDLLVDRYRWISIWFALA 260 Query: 262 YFTGLFMVYSLSSWLTKLMAMAGYSLGSALNFVLVFNFGAMAGAQPLQEVEDNLASLR 319 F L Y L +WL +LM AGY G AL F L N GA+ G+ D +R Sbjct: 261 TFVTLLAWYGLGTWLPRLMETAGYEFGHALMFTLALNLGAVIGSVVTAWAGDRFGPIR 318