BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0075.p (411 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 533 e-151 AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 364 e-100 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 354 2e-97 SMc00682 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 352 8e-97 PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 352 1e-96 >RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Rso Ralstonia solanacearum Length = 396 Score = 533 bits (1374), Expect = e-151, Method: Compositional matrix adjust. Identities = 250/395 (63%), Positives = 305/395 (77%), Gaps = 1/395 (0%) Query: 12 MNLIKEIETSHAEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGLVG 71 M LI EI + AE +RRD+HAHPEL F+E RT+D + A L WGI VHRGLG TGLVG Sbjct: 1 MKLIPEILAAQAEIQALRRDIHAHPELCFEEQRTSDLVAAKLAEWGIEVHRGLGKTGLVG 60 Query: 72 IIRNGRSTRAIGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNAT 131 +IRNG R IGLRADMDALP+ E N F H S++ GKMHACGHDGHTAMLL AA +L Sbjct: 61 VIRNGEGQR-IGLRADMDALPLAEANQFTHRSRHEGKMHACGHDGHTAMLLGAAHYLARH 119 Query: 132 RNFDGTVYLIFQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIM 191 RNF GTV+LIFQPAEEG GGA MI+DGLF++F ++V+ +HNWPG+P+G+FG G +M Sbjct: 120 RNFSGTVHLIFQPAEEGGGGAREMIRDGLFDRFPCDAVFGMHNWPGVPVGAFGTRVGPLM 179 Query: 192 ASSNEFKITLRGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQA 251 ASSNEF+I ++GKG+HAALPH+ DPV A + ALQ ++TRNKRP D AVLSITQ A Sbjct: 180 ASSNEFRIVIKGKGAHAALPHNGNDPVFVGAQMVSALQGVITRNKRPIDTAVLSITQFHA 239 Query: 252 GEASNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTIN 311 G+ASN+IP+ WIGGTVRTFS VLDLIE RM E+++ +A + C + F+RNYPPT+N Sbjct: 240 GDASNIIPNEAWIGGTVRTFSTAVLDLIERRMEEVAKAIAAAYDCSIDFTFHRNYPPTVN 299 Query: 312 HRAETAICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGH 371 ET EVMR++VG ++VDA+I+ + +EDFSFML+ KPGC+A IGNGDG HR+ GH Sbjct: 300 TERETLFAAEVMRELVGPDHVDANIDPTMGAEDFSFMLIEKPGCFAFIGNGDGDHREQGH 359 Query: 372 GLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLA 406 GLGPCMLHN SYDFND LLP+GATYWVRL E+ LA Sbjct: 360 GLGPCMLHNPSYDFNDELLPLGATYWVRLVEKFLA 394 >AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 430 Score = 364 bits (935), Expect = e-100, Method: Compositional matrix adjust. Identities = 190/404 (47%), Positives = 255/404 (63%), Gaps = 16/404 (3%) Query: 5 QNYTMQNMNLIKEIETSHAEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGI-PVHRG 63 +N M +N E++ E S R LH +PE+ + TA + L+++G+ + G Sbjct: 40 RNERMPILNRAAELQQ---EVSEWRHHLHENPEILYDVDNTAAFVAEKLKSFGVDEIVTG 96 Query: 64 LGGTGLVGIIR-NGRSTRAIGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLL 122 LG TG+VGIIR N R IG RADMDALPI E AS+ G MHACGHDGHTAMLL Sbjct: 97 LGRTGVVGIIRGNVPGNRTIGFRADMDALPILEETGKPWASKTAGAMHACGHDGHTAMLL 156 Query: 123 AAAWHLNATRNFDGTVYLIFQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGS 182 AA +L TRNF G++ +IFQPAEEG GA +M++DGL E+F ++ VY +HN PG+PLG+ Sbjct: 157 GAAKYLTETRNFAGSIAVIFQPAEEGGAGALAMVEDGLMERFGIDEVYGMHNMPGIPLGA 216 Query: 183 FGVNSGAIMASSNEFKITLRGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAA 242 F V G IMA+ ++F IT++G+G HAA PH +IDP+ A + LQ I +RN P + Sbjct: 217 FAVRKGGIMAAPDKFSITVKGRGGHAAQPHRTIDPITIGAQIVGNLQMIASRNADPIRSV 276 Query: 243 VLSITQMQAGEASNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIF 302 V+S+T+ AG + N+IP+ I GTVR+ V DL + R+++M+E +A G +AEV++ Sbjct: 277 VVSVTRFHAGSSHNIIPNEALIAGTVRSLDETVRDLAQERIKQMAEGIALANGAEAEVVY 336 Query: 303 NRNYPPTINHRAETAICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNG 362 R P T NH AET V V RDVVG NVD D++ ++ EDFSFML +PGC+ IGNG Sbjct: 337 ERYCPVTFNHSAETDYAVRVARDVVGEPNVDPDVDPSMAGEDFSFMLKERPGCFIFIGNG 396 Query: 363 DGGHRDHGHGLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLA 406 D LHN SYDFND + G +YWV+L E+ LA Sbjct: 397 DSAG-----------LHNPSYDFNDEAIAYGISYWVKLAEQRLA 429 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 354 bits (908), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 184/396 (46%), Positives = 245/396 (61%), Gaps = 10/396 (2%) Query: 12 MNLIKEIETSHAEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGLVG 71 M L I+ + E IRRDLHAHPEL +E RT+ I L+ G V G+ TG+VG Sbjct: 1 MKLSAAIDNAMPELVAIRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVG 60 Query: 72 IIRNGRSTRAIGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNAT 131 +RNG +R+IG+RAD+DALPIQE +AS PG MHACGHDGHTAMLL AA L Sbjct: 61 TLRNGTGSRSIGIRADIDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAER 120 Query: 132 RNFDGTVYLIFQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIM 191 RNFDGT++LIFQPAEE GGA M+ +GLF++F ++V+A+HN P +P G F + G IM Sbjct: 121 RNFDGTIHLIFQPAEENAGGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIM 180 Query: 192 ASSNEFKITLRGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQA 251 A+ +E +IT+ G+G H A P + DP+ A++ ALQTI+ RN P D +V+++ A Sbjct: 181 AAVDEARITVHGRGGHGAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHA 240 Query: 252 GEASNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTIN 311 G ASN+IP+ I +R+F V D +E R+R ++E A FG A V + R+Y TIN Sbjct: 241 GSASNIIPERAEIVVGIRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATIN 300 Query: 312 HRAETAICVEVMRDVVGAENVDADIEAP-LSSEDFSFMLLAKPGCYALIGNGDGGHRDHG 370 H+AET E GA+ V D+ P + SEDF++ML +PG Y +G+ G Sbjct: 301 HKAETDFLREAAIRFAGADKV-VDLARPFMGSEDFAYMLKERPGSYFFLGSRVTGEEKS- 358 Query: 371 HGLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLA 406 LH+ YDFND+LLPIGA +W L E LA Sbjct: 359 -------LHHPGYDFNDDLLPIGAAFWTELAEAYLA 387 >SMc00682 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 387 Score = 352 bits (903), Expect = 8e-97, Method: Compositional matrix adjust. Identities = 187/385 (48%), Positives = 251/385 (65%), Gaps = 13/385 (3%) Query: 24 EFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGI-PVHRGLGGTGLVGIIRNGRST-RA 81 E + RR LH +PEL F TA + L+ +G+ + GLG TG+VG+IR R R Sbjct: 13 EVTEWRRHLHQNPELLFAVENTAAFVERKLREFGVDEIVTGLGRTGVVGLIRGNRGPGRT 72 Query: 82 IGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNATRNFDGTVYLI 141 +GLRADMDALPI E + +S PGKMHACGHDGHTAMLL AA +L+ TRNF G V +I Sbjct: 73 VGLRADMDALPITEASGRAWSSTAPGKMHACGHDGHTAMLLGAAKYLSETRNFAGNVAVI 132 Query: 142 FQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASSNEFKITL 201 FQPAEEG GG M+KDG+ E+F +E VY +HN PGMP+G FG +G IMAS++EF IT+ Sbjct: 133 FQPAEEGGGGGNEMVKDGMMERFAIEEVYGMHNMPGMPVGHFGSRAGPIMASTDEFNITV 192 Query: 202 RGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEASNVIPDF 261 +G+G HAA PH +IDP+A A + ALQTI +R P + V+S+T+ AG A NVIP+ Sbjct: 193 KGRGGHAAQPHKTIDPIAIGAQIVNALQTIASRTVDPLASIVVSVTKFNAGFAHNVIPEQ 252 Query: 262 GWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTINHRAETAICVE 321 + GTVR + +V D E+R+R+++E++A +G +V + RNYP T+NH AE + Sbjct: 253 AVLAGTVRALTPEVRDTGEARIRQIAENIAGAYGATVDVWYGRNYPVTVNHAAEAGHALA 312 Query: 322 VMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGHGLGPCMLHNT 381 V + G NV+A ++ + EDFS+MLLA+PG + IGNGD LH+ Sbjct: 313 VAATIAGEGNVNAALDPMMGGEDFSYMLLARPGAFVFIGNGD-----------TAGLHHP 361 Query: 382 SYDFNDNLLPIGATYWVRLTERLLA 406 +YDFND +P G +YWV+L E LA Sbjct: 362 AYDFNDEAIPHGISYWVKLAETRLA 386 >PA4344 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Pae Pseudomonas aeruginosa Length = 406 Score = 352 bits (903), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 176/397 (44%), Positives = 249/397 (62%), Gaps = 10/397 (2%) Query: 10 QNMNLIKEIETSHAEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGL 69 ++ +++ +E + +R+D+HAHPEL F+E RTA + L+ WG VH G+G TG+ Sbjct: 3 RHRHIVAWLEEVADDLRSLRQDIHAHPELGFEERRTAALVAECLRGWGYEVHEGIGRTGV 62 Query: 70 VGIIRNGRSTRAIGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLN 129 VG++R G TR +GLRADMDALPI E ++S + G+MHACGHDGHTAMLL AA +L Sbjct: 63 VGVLRQGDGTRRLGLRADMDALPIVEATGLGYSSCHGGRMHACGHDGHTAMLLGAARYLA 122 Query: 130 ATRNFDGTVYLIFQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGA 189 ATR FDGT+ LIFQPAEEG+GGA +M+ DGL E+F ++++ +HN PG+ G G +G Sbjct: 123 ATRRFDGTLVLIFQPAEEGQGGAEAMLADGLLERFPCDALFGMHNMPGLEAGHLGFRAGP 182 Query: 190 IMASSNEFKITLRGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQM 249 +MAS + +TL G G H ++PH S+DP+ A ++ ALQ+++ RN P AAV+++ + Sbjct: 183 MMASQDLLSVTLEGVGGHGSMPHLSVDPLLAASSAVMALQSVVARNVDPQKAAVVTVGAL 242 Query: 250 QAGEASNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPT 309 QAGEA+NVIP + ++R V + + R+R++ E A +GC A + YP Sbjct: 243 QAGEAANVIPQRAVLRLSLRALDGQVREQVLQRVRQIIELQAASYGCQASIEHYPAYPVL 302 Query: 310 INHRAETAICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDH 369 +N ET +V ++ GAE VD D + SEDF++ML PG Y IGNG Sbjct: 303 VNSVEETEFARQVGVELAGAEQVDGDTPKLMGSEDFAWMLQRCPGSYLFIGNGR------ 356 Query: 370 GHGLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLA 406 G M+HN +YDFND++L GA YW L E LA Sbjct: 357 ----GRPMVHNPAYDFNDDILVRGAAYWGALAETWLA 389