BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0090.p (382 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20510 [MR] COG4948 L-alanine-DL-glutamate epimerase and relat... 629 e-180 RSc2751 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 484 e-136 dgoA_2 [MR] COG4948 L-alanine-DL-glutamate epimerase and related... 482 e-136 STM3828 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 475 e-134 mll7085 [MR] COG4948 L-alanine-DL-glutamate epimerase and relate... 244 3e-64 >SMb20510 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sme Sinorhizobium meliloti Length = 382 Score = 629 bits (1622), Expect = e-180, Method: Compositional matrix adjust. Identities = 293/382 (76%), Positives = 339/382 (88%) Query: 1 MKITKLTTFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNI 60 MKITKLTT+IVPPRW FLKIETDEG+VGWGEPVVEGRA +V AAV EL+DYL+GKDP I Sbjct: 1 MKITKLTTYIVPPRWLFLKIETDEGVVGWGEPVVEGRALTVEAAVHELSDYLVGKDPFLI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSWI 120 EDHW VLYRGGFYRGGAIHMSALAGIDQALWDIKGKALG PV+ LLGG RD I+VYSWI Sbjct: 61 EDHWNVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGQPVHSLLGGQCRDRIKVYSWI 120 Query: 121 GGDRPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGI 180 GGDRP+D A A++ VARGF A+K+NG EE+Q +DT +K++ + + +R+A+GP+VGI Sbjct: 121 GGDRPSDVANNAREVVARGFKAIKLNGCEEMQIVDTNEKIDKAVETIGLIRDAIGPHVGI 180 Query: 181 GVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSR 240 GVDFHGRVH+PMAKVL KELE +KLMFIEEPVLSEN EAL+EIA+ TPIALGERLYSR Sbjct: 181 GVDFHGRVHRPMAKVLAKELEPFKLMFIEEPVLSENREALREIANHCSTPIALGERLYSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVD 300 WDFK +LS+G+VDIIQPD+SHAGGITE RKIA MAEAYDVALA HCPLGPIALAACLQVD Sbjct: 241 WDFKSVLSDGFVDIIQPDLSHAGGITECRKIAAMAEAYDVALAPHCPLGPIALAACLQVD 300 Query: 301 AVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQR 360 AVSYNAFIQEQSLGIHYNE+ND+LDY+++ +VF+Y+DG+V+IP GPGLGIE++E YV +R Sbjct: 301 AVSYNAFIQEQSLGIHYNEANDILDYISNKEVFAYEDGFVSIPQGPGLGIEVDEAYVMER 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 A+ GHRWRNP+WRH DGS AEW Sbjct: 361 AKEGHRWRNPVWRHSDGSVAEW 382 >RSc2751 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Rso Ralstonia solanacearum Length = 382 Score = 484 bits (1245), Expect = e-136, Method: Compositional matrix adjust. Identities = 237/382 (62%), Positives = 288/382 (75%) Query: 1 MKITKLTTFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNI 60 MKIT+LTT+ +PPRW FLKIETDEG+ GWGEPV+EGRA +V AAV ELADYL+G+DPR I Sbjct: 1 MKITRLTTYRLPPRWMFLKIETDEGVAGWGEPVIEGRARTVEAAVHELADYLVGQDPRRI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSWI 120 D W LYRGGFYRGG I MSA+AGIDQALWDIKGK LG+PV +LLGG VRD +R YSW+ Sbjct: 61 NDLWQTLYRGGFYRGGPILMSAIAGIDQALWDIKGKVLGVPVYELLGGLVRDRMRTYSWV 120 Query: 121 GGDRPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGI 180 GGDRPAD K A GF K+NG EE+ ID+ V+ +A VA +R A G V Sbjct: 121 GGDRPADVIAGMKALQAGGFDHFKLNGCEEMGIIDSARAVDAAVARVAEIRAAFGNTVEF 180 Query: 181 GVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSR 240 G+DFHGRV PMAKVL++ LE Y+ +FIEEPVL+E E+ +A T PIA GER++SR Sbjct: 181 GLDFHGRVSAPMAKVLIRALEPYRPLFIEEPVLAEQAESYARLAAQTHLPIAAGERMFSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVD 300 ++FKR+L G + I+QPD+SHAGGITE KIA MAEAYDVALA HCPLGPIALAACL +D Sbjct: 241 FEFKRVLEAGGLAILQPDLSHAGGITECLKIAGMAEAYDVALAPHCPLGPIALAACLHID 300 Query: 301 AVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQR 360 VS+NA +QEQS+GIHYN+ +LLDYV + F+ + GY+ P PGLG++I+E V +R Sbjct: 301 FVSWNATLQEQSMGIHYNQGAELLDYVRNKADFALEGGYIRPPRLPGLGVDIDEALVIER 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 + WRNP+WRH DGS AEW Sbjct: 361 SRSAPDWRNPVWRHADGSVAEW 382 >dgoA_2 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Eco Escherichia coli K12 Length = 477 Score = 482 bits (1240), Expect = e-136, Method: Compositional matrix adjust. Identities = 233/382 (60%), Positives = 281/382 (73%) Query: 1 MKITKLTTFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNI 60 MKITK+TT+ +PPRW FLKIETDEG+VGWGEPV+EGRA +V AAV EL DYLIG+DP I Sbjct: 96 MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI 155 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSWI 120 D W V+YR GFYRGG I MSA+AGIDQALWDIKGK L PV QL+GG VRD I+ YSW+ Sbjct: 156 NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 215 Query: 121 GGDRPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGI 180 GGDRPAD K GF K+NG EEL ID V+ + VA +REA G + Sbjct: 216 GGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF 275 Query: 181 GVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSR 240 G+DFHGRV PMAKVL+KELE Y+ +FIEEPVL+E E ++A T P+A GER++SR Sbjct: 276 GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR 335 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVD 300 +DFKR+L G + I+QPD+SHAGGITE KIA MAEAYDV LA HCPLGPIALAACL +D Sbjct: 336 FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID 395 Query: 301 AVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQR 360 VSYNA +QEQS+GIHYN+ +LLD+V + + FS G+ PGLG+EI+E V + Sbjct: 396 FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF 455 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 ++ WRNP+WRH+D S AEW Sbjct: 456 SKNAPDWRNPLWRHEDNSVAEW 477 >STM3828 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Sty Salmonella typhimurium LT2 Length = 382 Score = 475 bits (1222), Expect = e-134, Method: Compositional matrix adjust. Identities = 231/382 (60%), Positives = 278/382 (72%) Query: 1 MKITKLTTFIVPPRWCFLKIETDEGIVGWGEPVVEGRAHSVAAAVEELADYLIGKDPRNI 60 MKIT +TT+ +PPRW FLKIETDEG+VGWGEPV+EGRA +V AAV E ADYLIGKDP I Sbjct: 1 MKITHITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHEFADYLIGKDPARI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRVYSWI 120 D W V+YR GFYRGG I MSA+AGIDQALWDIKGK L PV QL+GG VRD I+ YSW+ Sbjct: 61 NDLWQVMYRAGFYRGGPIMMSAIAGIDQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWV 120 Query: 121 GGDRPADTAKAAKDAVARGFTAVKMNGTEELQFIDTYDKVELTLANVAAVREAVGPNVGI 180 GGDRPAD + GF K+NG EE+ ID V+ + VA +REA G + Sbjct: 121 GGDRPADVIDGIEKLRGIGFDTFKLNGCEEMGVIDNSRAVDAAVNTVAQIREAFGSEIEF 180 Query: 181 GVDFHGRVHKPMAKVLVKELEQYKLMFIEEPVLSENYEALKEIAHLTGTPIALGERLYSR 240 G+DFHGRV PMAKVL+KELE Y+ +FIEEPVL+E E +A T PIA GER++SR Sbjct: 181 GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPRLAAQTHIPIAAGERMFSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHCPLGPIALAACLQVD 300 ++FKR+L G + I+QPD+SHAGGITE KIA MAEAYDVALA HCPLGPIALAACL +D Sbjct: 241 FEFKRVLDAGGLAILQPDLSHAGGITECYKIAGMAEAYDVALAPHCPLGPIALAACLHID 300 Query: 301 AVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGPGLGIEINEEYVRQR 360 VS NA QEQS+GIHYN+ +LLD+V + + FS G+ PGLG++I+E V + Sbjct: 301 FVSRNAVFQEQSMGIHYNKGAELLDFVKNKEDFSMDGGFFKPLTKPGLGVDIDEARVIEL 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 ++ WRNP+WRH DGS AEW Sbjct: 361 SKSAPDWRNPLWRHADGSVAEW 382 >mll7085 [MR] COG4948 L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily Mlo Mesorhizobium loti Length = 390 Score = 244 bits (622), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 153/399 (38%), Positives = 223/399 (55%), Gaps = 26/399 (6%) Query: 1 MKITKLTTFIVPP---RWCFLKIETDE-GIVGWGEPVVEGRAHSVAAAVEELADYLIGKD 56 MKIT + + W F+++ETDE G+ GWGE +E + +V A+++L LIG+D Sbjct: 1 MKITAIKALVCNAEMRNWVFVRVETDEPGLFGWGEATLEWKTRAVVGALQDLEALLIGQD 60 Query: 57 PRNIEDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKALGLPVNQLLGGPVRDSIRV 116 PRN+E + +L R GF+R G MSA++GI+ ALWDI GK+LG+PV +LLGG VRD +R Sbjct: 61 PRNVEQCYQILTRHGFWRLGVEGMSAVSGIEIALWDILGKSLGVPVWRLLGGQVRDYLRT 120 Query: 117 YSWIG-GDRPADTAKAAKDAVAR--------GFTAVKMNGTEELQFIDTYDKVELTLANV 167 Y+ +G GD A + D + G+ AVK+ ++ V+ V Sbjct: 121 YTHLGLGDMKAVYESGSADTIVERGRIVTQAGYDAVKVVCIPYTHYVSPAKAVDSVADMV 180 Query: 168 AAVREAVGPNVGIGVDFHGRVHKPMAKV-LVKELEQYKLMFIEEPVLSENYEALKEIAHL 226 A+REAVGP++ I VDFHGR A + +K +E ++MF EEPV E+ L I Sbjct: 181 GALREAVGPDIDIMVDFHGRPASVNAAMDYLKAIEPARIMFAEEPVPPEDVAGLAHITAR 240 Query: 227 TGTPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIATMAEAYDVALALHC 286 + PIA GERL R +F + ++ +I QPD+ H GG+ ET+KIA +AE + LA H Sbjct: 241 SAIPIASGERLIGRREFTQSVAARAFNIAQPDICHTGGLLETKKIAALAETAGIGLAPHN 300 Query: 287 PLGPIALAACLQVDAVSYNAFIQEQSLGIHYNESNDLLDYVTDADVFSYKDGYVTIPNGP 346 PLGPIA A L + N IQE+ G +D++ + D K G +P+ P Sbjct: 301 PLGPIAGVAALHFGISTPNVIIQEEMSG-AVAWYDDVVTWPID-----RKPGRWDVPDLP 354 Query: 347 GLGIEINEEYVRQ---RAEVGHRWRNPIWRHKDGSFAEW 382 GLG+ +NE+ + + + EV H RN + DG+ +W Sbjct: 355 GLGVTVNEDVIARHPFKQEVLHA-RNAV--MPDGTVVDW 390