BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0068.p (227 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1086 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 276 2e-74 PA2471 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 273 3e-73 STM2177 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 270 1e-72 Z3392 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene... 270 2e-72 ECs3029 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 270 2e-72 >RSc1086 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Rso Ralstonia solanacearum Length = 232 Score = 276 bits (707), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 135/232 (58%), Positives = 167/232 (71%), Gaps = 5/232 (2%) Query: 1 MSYAIPVPAVTTVPVVGG-DPFPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDTI 59 M+Y PA+T +PV G + F VRR+YCVGRNYAAHAREMG DP+RE PFFF KP D I Sbjct: 1 MTYVFTPPALTAIPVAGSSEQFAVRRVYCVGRNYAAHAREMGFDPDREPPFFFCKPADAI 60 Query: 60 VPTDS----AVPYPSKTNDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDL 115 VP A+PYP++T +Y +E ELV +GK G D+ D ALD V+GYAVGLDMTRRDL Sbjct: 61 VPVADGQTLALPYPTQTQNYHYEAELVAVIGKAGADIAVDDALDHVWGYAVGLDMTRRDL 120 Query: 116 QGEAKKLARPWDMGKGFDHSAPCAPLLPVSRIGHPEQGAIWLKVNGEVRQQGDLSDLIWN 175 Q + +++ RPW++GK FD SAP P+ S IGHP Q IWL VNGE +Q+ D+S LIW+ Sbjct: 121 QMKMREMGRPWEIGKAFDVSAPVGPIRRASDIGHPRQAGIWLTVNGEAKQRSDVSHLIWS 180 Query: 176 VAETISYLSGLVELFPGDLIYTGTPEGVGAVVKGDKLHGHVDGLVDLNITIA 227 VAET++ LS L PGD+I+TGTPEGVGAV GD + VDGL +LN+ IA Sbjct: 181 VAETVACLSQFFRLEPGDVIFTGTPEGVGAVKPGDTIATGVDGLGELNVRIA 232 >PA2471 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Pae Pseudomonas aeruginosa Length = 232 Score = 273 bits (697), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 130/231 (56%), Positives = 171/231 (74%), Gaps = 5/231 (2%) Query: 1 MSYAIPVPAVTTVPVVGGDP-FPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDTI 59 M++ A ++ + G D FPVRR+YCVGRNYAAHAREMG DP RE PFFF KP D + Sbjct: 1 MNHLFAPEAPVSLTIHGSDQTFPVRRVYCVGRNYAAHAREMGFDPEREPPFFFCKPADAV 60 Query: 60 VPTDSA----VPYPSKTNDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDL 115 VP + + YPS+T +Y +EIELV A+GKGG D+P +QA + V+GYAVGLDMTRRDL Sbjct: 61 VPVAAGSTLELAYPSQTGNYHYEIELVAAIGKGGCDIPLEQAEEHVWGYAVGLDMTRRDL 120 Query: 116 QGEAKKLARPWDMGKGFDHSAPCAPLLPVSRIGHPEQGAIWLKVNGEVRQQGDLSDLIWN 175 Q +++ RPW++GK FD SAP PL P S++GHP AI L+V+GE RQ+ D+ LIW+ Sbjct: 121 QMRMREMGRPWEIGKAFDRSAPIGPLYPASQVGHPRHAAISLQVDGEDRQRSDIDQLIWS 180 Query: 176 VAETISYLSGLVELFPGDLIYTGTPEGVGAVVKGDKLHGHVDGLVDLNITI 226 VAET+SYLS EL PGDL++TGTPEGVGAV +G+++ G +DGL +L++ + Sbjct: 181 VAETVSYLSRFFELRPGDLVFTGTPEGVGAVERGERMLGAIDGLGELSVRV 231 >STM2177 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sty Salmonella typhimurium LT2 Length = 233 Score = 270 bits (691), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 133/230 (57%), Positives = 167/230 (72%), Gaps = 5/230 (2%) Query: 2 SYAIPVPAVTTVPVVGGDP-FPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDTIV 60 Y A TVPV G D FPVRR+YCVGRNYAAHAREMG DP+RE PFFF KP D +V Sbjct: 3 KYVFQPQAPVTVPVAGSDEQFPVRRVYCVGRNYAAHAREMGFDPDREPPFFFCKPADAVV 62 Query: 61 PTDSA----VPYPSKTNDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDLQ 116 P + +PYP++T++Y +EIELVVA+GK G D+P ++A + V+GYA GLDMTRRD Q Sbjct: 63 PVAAGDTLELPYPAQTDNYHYEIELVVAIGKKGSDIPLEKAHEYVWGYATGLDMTRRDRQ 122 Query: 117 GEAKKLARPWDMGKGFDHSAPCAPLLPVSRIGHPEQGAIWLKVNGEVRQQGDLSDLIWNV 176 E +++ RPW++GK FD SAP APL + + + IWL+VNGE Q+ D+ LIW+V Sbjct: 123 MEMRQMGRPWEIGKAFDLSAPIAPLHKAAETHNVDNAPIWLQVNGEDHQRSDIRHLIWSV 182 Query: 177 AETISYLSGLVELFPGDLIYTGTPEGVGAVVKGDKLHGHVDGLVDLNITI 226 ETISYLSG EL PGDLI+TGTPEGVGAVVKGD + G+V+GL + + I Sbjct: 183 NETISYLSGFFELQPGDLIFTGTPEGVGAVVKGDVITGNVEGLTPIAVKI 232 >Z3392 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) EcZ Escherichia coli O157:H7 EDL933 Length = 233 Score = 270 bits (690), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 135/230 (58%), Positives = 166/230 (72%), Gaps = 5/230 (2%) Query: 2 SYAIPVPAVTTVPVVGGDP-FPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDTIV 60 Y A TVPVVG D FPVRR+YCVGRNYAAHAREMG DP+RE PFFF KP D +V Sbjct: 3 QYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGFDPDREPPFFFCKPADAVV 62 Query: 61 PTDS----AVPYPSKTNDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDLQ 116 P + A+PYP++T++Y +EIELVVA+GK G D+P + A + V+GYA GLDMTRRD Q Sbjct: 63 PVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEHAHEYVWGYATGLDMTRRDRQ 122 Query: 117 GEAKKLARPWDMGKGFDHSAPCAPLLPVSRIGHPEQGAIWLKVNGEVRQQGDLSDLIWNV 176 E +++ RPW++GK FD SAP APL IG + IWL+VNGE Q+ D+ LIW+V Sbjct: 123 MEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAPIWLQVNGEDHQRSDIRHLIWSV 182 Query: 177 AETISYLSGLVELFPGDLIYTGTPEGVGAVVKGDKLHGHVDGLVDLNITI 226 ETI YLSG EL PGDLI+TGTPEGVG VVKGD + G+V+GL + + I Sbjct: 183 NETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVITGNVEGLTPIAVKI 232 >ECs3029 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Ecs Escherichia coli O157:H7 Length = 233 Score = 270 bits (690), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 135/230 (58%), Positives = 166/230 (72%), Gaps = 5/230 (2%) Query: 2 SYAIPVPAVTTVPVVGGDP-FPVRRIYCVGRNYAAHAREMGHDPNREAPFFFMKPTDTIV 60 Y A TVPVVG D FPVRR+YCVGRNYAAHAREMG DP+RE PFFF KP D +V Sbjct: 3 QYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGFDPDREPPFFFCKPADAVV 62 Query: 61 PTDS----AVPYPSKTNDYQHEIELVVALGKGGRDVPADQALDLVFGYAVGLDMTRRDLQ 116 P + A+PYP++T++Y +EIELVVA+GK G D+P + A + V+GYA GLDMTRRD Q Sbjct: 63 PVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEHAHEYVWGYATGLDMTRRDRQ 122 Query: 117 GEAKKLARPWDMGKGFDHSAPCAPLLPVSRIGHPEQGAIWLKVNGEVRQQGDLSDLIWNV 176 E +++ RPW++GK FD SAP APL IG + IWL+VNGE Q+ D+ LIW+V Sbjct: 123 MEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAPIWLQVNGEDHQRSDIRHLIWSV 182 Query: 177 AETISYLSGLVELFPGDLIYTGTPEGVGAVVKGDKLHGHVDGLVDLNITI 226 ETI YLSG EL PGDLI+TGTPEGVG VVKGD + G+V+GL + + I Sbjct: 183 NETICYLSGFFELQPGDLIFTGTPEGVGPVVKGDVITGNVEGLTPIAVKI 232