BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0744.p (226 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC1662 [U] COG2095 Multiple antibiotic transporter Ccr Caulobact... 102 7e-22 STM1521 [U] COG2095 Multiple antibiotic transporter Sty Salmonel... 93 6e-19 marC [U] COG2095 Multiple antibiotic transporter Eco Escherichia... 91 2e-18 PH0760 [U] COG2095 Multiple antibiotic transporter Pho Pyrococcu... 91 2e-18 ECs2136 [U] COG2095 Multiple antibiotic transporter Ecs Escheric... 91 2e-18 >CC1662 [U] COG2095 Multiple antibiotic transporter Ccr Caulobacter vibrioides Length = 228 Score = 102 bits (254), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 71/213 (33%), Positives = 120/213 (56%), Gaps = 5/213 (2%) Query: 8 AVLLIPVTLLPILNPLGIAPVFANLLGDVPRQIENRVARQVAINCWFLLVGAIFIGSHVL 67 A LL L I+NP+G A +F +G R ++AR + + +L+G++ +G ++L Sbjct: 14 AFLLAFPALFSIVNPVGAALIFDQAMGGRSRPERLKLARAIGFYAFLVLLGSLLLGGYIL 73 Query: 68 AFFGISLPIVRIGGGILVAVSGWKLLSDGGNDAAIPKQVAKAYDDESNEELKARSFYPIS 127 FFG+S+ +R+ GG++VA+ W LL D + + K ES + +F+P++ Sbjct: 74 NFFGVSIGALRVAGGLVVAIRAWGLLMDPEQN-----EDRKVNQTESAQSHHDVAFFPLT 128 Query: 128 FPLTVGPGTIAASITLGANTPARLVDWVMSIGSAALGSALTVLVIYLCYRSANKIVSLLG 187 P T GPG+I+ +I L + P A+L +AL ++++LCY ++ +++ LLG Sbjct: 129 MPFTTGPGSISVAIALSSQRPTEGDAVAPFFIGASLAAALVAVLVWLCYSASGQVMRLLG 188 Query: 188 RLGTMVVLRLSAFILLCIGIQIFWSGFTGLLVE 220 G V+ RL AF+LLCIG+QI SG L+ + Sbjct: 189 PSGARVLSRLVAFLLLCIGVQIIASGVENLVAK 221 >STM1521 [U] COG2095 Multiple antibiotic transporter Sty Salmonella typhimurium LT2 Length = 221 Score = 92.8 bits (229), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 68/211 (32%), Positives = 115/211 (54%), Gaps = 6/211 (2%) Query: 6 LKAVLLIPVTLLPILNPLGIAPVFANLLGDVPRQIENRVARQVAINCWFLLVGAIFIGSH 65 KA+ L V LLP+ NPL +F L G++ NR + ++ + +++ A + G Sbjct: 5 FKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQSYMASVYVFAIMMVAYYAGQL 64 Query: 66 VLAFFGISLPIVRIGGGILVAVSGWKLLSDGGNDAAIPKQVAKAYDDESNEELKAR-SFY 124 V+ FGIS+P +RI GG++VA G+++L P+ AK+ +E +E A +F Sbjct: 65 VMNTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAHESPE--AKSKSEELADEPTANIAFV 122 Query: 125 PISFPLTVGPGTIAASITLGANTP--ARLVDWVMSIGSAALGSALTVLVIYLCYRSANKI 182 P++ P T GPGTIA I+ + DWV+ + + A+ V +++ C RS+ I Sbjct: 123 PLAMPSTAGPGTIAMIISSASTVRHGGEFPDWVIMVAPPIIFLAVAV-ILWGCLRSSGAI 181 Query: 183 VSLLGRLGTMVVLRLSAFILLCIGIQIFWSG 213 + L+G+ G + RL F+L+C+G+Q +G Sbjct: 182 MRLVGKGGIEAISRLMGFLLVCMGVQFIING 212 >marC [U] COG2095 Multiple antibiotic transporter Eco Escherichia coli K12 Length = 221 Score = 91.3 bits (225), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 71/216 (32%), Positives = 119/216 (55%), Gaps = 7/216 (3%) Query: 1 MLIIFLKAVLLIPVTLLPILNPLGIAPVFANLLGDVPRQIENRVARQVAINCWFLLVGAI 60 ML +F KA+ L V LLP+ NPL +F L G++ NR + ++ + +++ A Sbjct: 1 MLDLF-KAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQSLMASVYVFAIMMVAY 59 Query: 61 FIGSHVLAFFGISLPIVRIGGGILVAVSGWKLLSDGGNDAAIPKQVAKAYDDESNEELKA 120 + G V+ FGIS+P +RI GG++VA G+++L AI AK+ +E +E A Sbjct: 60 YAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFP--QQKAIDSPEAKSKSEELEDEPSA 117 Query: 121 R-SFYPISFPLTVGPGTIAASITLGAN--TPARLVDWVMSIGSAALGSALTVLVIYLCYR 177 +F P++ P T GPGTIA I+ + + DWV+ + + L L ++++ R Sbjct: 118 NIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMV-APPLIFFLVAVILWGSLR 176 Query: 178 SANKIVSLLGRLGTMVVLRLSAFILLCIGIQIFWSG 213 S+ I+ L+G+ G + RL F+L+C+G+Q +G Sbjct: 177 SSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIING 212 >PH0760 [U] COG2095 Multiple antibiotic transporter Pho Pyrococcus horikoshii Length = 201 Score = 91.3 bits (225), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 59/212 (27%), Positives = 105/212 (49%), Gaps = 20/212 (9%) Query: 6 LKAVLLIPVTLLPILNPLGIAPVFANLLGDVPRQIENRVARQVAINCWFLLVGAIFIGSH 65 +K +++ + I NP+G PVF +++G +P +I++ VA++V+I + L +G Sbjct: 5 IKTFMILYTGMFAITNPIGAVPVFMSVVGHLPAEIKHEVAKKVSITVFITLTVFALVGQW 64 Query: 66 VLAFFGISLPIVRIGGGILVAVSGWKLLSDGGNDAAIPKQVAKAYDDESNEELKARSFYP 125 + FFG S+ I GGIL+ G ++LS +++ DE + L+ + P Sbjct: 65 IFKFFGSSIDAFAIAGGILLFRMGMEMLSG---------KLSSVKIDEEDVTLEEVAVIP 115 Query: 126 ISFPLTVGPGTIAA-SITLGANTPARLVDWVMSIGSAALGSALTVLVIYLCYRSANKIVS 184 ++ PL GPG I + + +P ++ +++IG L Y S NKI+ Sbjct: 116 LAIPLISGPGAITTVMLYMTKESPGIVILTIIAIG----------LTTYGILYSGNKIIE 165 Query: 185 LLGRLGTMVVLRLSAFILLCIGIQIFWSGFTG 216 LGR+G V R+ IL + +Q+ +G G Sbjct: 166 RLGRVGVKVTTRMMGLILTSMAMQMIINGIKG 197 >ECs2136 [U] COG2095 Multiple antibiotic transporter Ecs Escherichia coli O157:H7 Length = 221 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 71/216 (32%), Positives = 119/216 (55%), Gaps = 7/216 (3%) Query: 1 MLIIFLKAVLLIPVTLLPILNPLGIAPVFANLLGDVPRQIENRVARQVAINCWFLLVGAI 60 ML +F KA+ L V LLP+ NPL +F L G++ NR + ++ + +++ A Sbjct: 1 MLDLF-KAIGLGLVVLLPLANPLTTVALFLGLAGNMNGAERNRQSLMASVYVFAIMMVAY 59 Query: 61 FIGSHVLAFFGISLPIVRIGGGILVAVSGWKLLSDGGNDAAIPKQVAKAYDDESNEELKA 120 + G V+ FGIS+P +RI GG++VA G+++L AI AK+ +E +E A Sbjct: 60 YAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFP--QQKAIDSPEAKSKSEELEDEPSA 117 Query: 121 R-SFYPISFPLTVGPGTIAASITLGAN--TPARLVDWVMSIGSAALGSALTVLVIYLCYR 177 +F P++ P T GPGTIA I+ + + DWV+ + + L L ++++ R Sbjct: 118 NIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMV-APPLIFFLVAVILWGSLR 176 Query: 178 SANKIVSLLGRLGTMVVLRLSAFILLCIGIQIFWSG 213 S+ I+ L+G+ G + RL F+L+C+G+Q +G Sbjct: 177 SSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIING 212