BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0347.p (192 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4098 [IQR] COG1028 Dehydrogenases with different specificities... 143 2e-34 SMc02336 [IQR] COG1028 Dehydrogenases with different specificiti... 121 8e-28 TM0441 [IQR] COG1028 Dehydrogenases with different specificities... 108 9e-24 SMb21348 [IQR] COG1028 Dehydrogenases with different specificiti... 107 1e-23 aq_1716 [IQR] COG1028 Dehydrogenases with different specificitie... 105 6e-23 >PA4098 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 241 Score = 143 bits (361), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 82/241 (34%), Positives = 120/241 (49%), Gaps = 49/241 (20%) Query: 1 MTFTPNLFQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATEEFSGTGVTLAEL 60 M F +++ G+ LVTGG+ GIG A+A Q A +GA+V+A GL + EL Sbjct: 1 MVFQHDIYAGQQVLVTGGSSGIGAAIAMQFAELGAEVVALGLDADGVHAPRHPRIRREEL 60 Query: 61 NVASEDSVRNLFAGIQQLDIVINCAGIIKRGAEHEVDVFEQVIAVNLTGTMRVCSAARAL 120 ++ ++ LF + +LD+++N AGI + E+++ FE+V+ +NL+ M AR L Sbjct: 61 DITDSQRLQRLFEALPRLDVLVNNAGISRDREEYDLATFERVLRLNLSAAMLASQLARPL 120 Query: 121 V-------------------------------------------------VNAVAPGWIA 131 + VNA+APGWI Sbjct: 121 LAQRGGSILNIASMYSTFGSADRPAYSASKGAIVQLTRSLACEYAAERIRVNAIAPGWID 180 Query: 132 TPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGGYLV 191 TPL L+ D + I+QRTPL RWG ++ + AAFLC P ASF+TGAV+ VDGGYL Sbjct: 181 TPLGAGLKADVEATRRIMQRTPLARWGEAPEVASAAAFLCGPGASFVTGAVLAVDGGYLC 240 Query: 192 S 192 + Sbjct: 241 A 241 >SMc02336 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 252 Score = 121 bits (304), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 79/243 (32%), Positives = 118/243 (48%), Gaps = 53/243 (21%) Query: 2 TFTPNLFQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLP----PFATEEFSGTGVTL 57 TF P+LF GK LVTGG+ GIG A+A+ A +G VIA G A + T + Sbjct: 7 TFRPDLFAGKDILVTGGSSGIGLAIAQGFARLGGSVIALGSSETKLKAAAADPENTAIRF 66 Query: 58 AELNVASEDSVRNLFAGIQQLDIVINCAGIIKRGAEHEVDVFEQVIAVNLTGTMR----- 112 ++V +++ +++LD+++N AGI + AE E + +V+ VNL MR Sbjct: 67 ERVDVRDPEAINAFARTLRKLDVLVNGAGIARPEAEFEDATYMEVMDVNLNSQMRFAMAV 126 Query: 113 --VCSAARALV------------------------------------------VNAVAPG 128 + A+R ++ VNA++PG Sbjct: 127 LPLLKASRGVILNTASMLSYVSDPLVPAYGASKSGVLGLTRHLAHAFGPDGIRVNAISPG 186 Query: 129 WIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVPVDGG 188 + T +TK L DP + I QR+ L RWGT +D+ A FL +PAA F+TGA +PVDGG Sbjct: 187 YHKTDMTKGLWTDPVPAEKIAQRSALKRWGTVDDLVGTALFLASPAAKFITGADLPVDGG 246 Query: 189 YLV 191 ++V Sbjct: 247 FVV 249 >TM0441 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 255 Score = 108 bits (269), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 77/249 (30%), Positives = 112/249 (44%), Gaps = 70/249 (28%) Query: 8 FQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATEEFSGTGVTLAE-------- 59 +G+ ALVTGG++G+G +A+ LA G V+ A EE S L E Sbjct: 7 LRGRVALVTGGSRGLGFGIAQGLAEAGCSVVVASRN---LEEASEAAQKLTEKYGVETMA 63 Query: 60 --LNVASEDSVRNLFAGIQQ----LDIVINCAGIIKR--GAEHEVDVFEQVIAVNLTGTM 111 +V++ + V+ L +++ LD V+N AGI +R E +D F QVI VNL GT Sbjct: 64 FRCDVSNYEEVKKLLEAVKEKFGKLDTVVNAAGINRRHPAEEFPLDEFRQVIEVNLFGTY 123 Query: 112 RVCSAARALV-------------------------------------------------- 121 VC A +L+ Sbjct: 124 YVCREAFSLLRESDNPSIINIGSLTVEEVTMPNISAYAASKGGVASLTKALAKEWGRYGI 183 Query: 122 -VNAVAPGWIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTG 180 VN +APGW T +T+A+ DP + +L+R PL R G PED+ VA FL + A ++TG Sbjct: 184 RVNVIAPGWYRTKMTEAVFSDPEKLDYMLKRIPLGRTGVPEDLKGVAVFLASEEAKYVTG 243 Query: 181 AVVPVDGGY 189 ++ VDGG+ Sbjct: 244 QIIFVDGGW 252 >SMb21348 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 254 Score = 107 bits (268), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 85/250 (34%), Positives = 115/250 (46%), Gaps = 65/250 (26%) Query: 5 PNLFQ--GKTALVTGGTQGIGGAVARQLAAMGAKVIAAGLPPFATEE--FSGTGVTL--A 58 P+ F G+ +VTG GIG +A +A G VI G E + G T A Sbjct: 4 PSRFSLAGRRVVVTGANTGIGQGIAVSIARAGGTVIGVGRSAMDETEKKVTDAGGTFIPA 63 Query: 59 ELNVASEDSVRNLFAGIQQ----LDIVINCAGIIKRGAE---HEVDVFEQVIAVNLT--- 108 ++A ++ R+L G+ L ++N AGII+R E D ++ VI VNL Sbjct: 64 HADLADREATRSLIDGLWNAHGPLQGLVNNAGIIRRADAVDFTEAD-WDDVIDVNLRSLF 122 Query: 109 -----------------------------GTMRVCS-------------------AARAL 120 G +RV S AA+ + Sbjct: 123 FLSQAFGRRIIAEESRGKIVNIASLLSFQGGIRVASYTASKHGALGITRLLACEWAAKGI 182 Query: 121 VVNAVAPGWIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTG 180 VNAVAPG++ T T+AL+ D RS IL+R P RWGTP+DIG+ A FL PA+ +M G Sbjct: 183 NVNAVAPGYVVTNNTEALRADEKRSASILERIPAGRWGTPDDIGDAAVFLLAPASDYMHG 242 Query: 181 AVVPVDGGYL 190 AVVPVDGG+L Sbjct: 243 AVVPVDGGWL 252 >aq_1716 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Aae Aquifex aeolicus Length = 248 Score = 105 bits (262), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 79/244 (32%), Positives = 115/244 (47%), Gaps = 65/244 (26%) Query: 8 FQGKTALVTGGTQGIGGAVARQLAAMGAKVIAAGL-----PPFATEEFSGTGVTL--AEL 60 QGK +LVTG T+GIG A+A +LA+ G+ VI G A E + GV E+ Sbjct: 5 LQGKVSLVTGSTRGIGRAIAEKLASAGSTVIITGTSGERAKAVAEEIANKYGVKAHGVEM 64 Query: 61 NVASEDSVRNLFAGIQQL----DIVINCAGIIKRGAEHEVDV--FEQVIAVNLTGTMRVC 114 N+ SE+S+ F I L DI++N AGI + + + +E+V+ VNLTGT V Sbjct: 65 NLLSEESINKAFEEIYNLVDGIDILVNNAGITRDKLFLRMSLLDWEEVLKVNLTGTFLVT 124 Query: 115 S--------------------------------------------------AARALVVNA 124 A R ++VNA Sbjct: 125 QNSLRKMIKQRWGRIVNISSVVGFTGNVGQVNYSTTKAGLIGFTKSLAKELAPRNVLVNA 184 Query: 125 VAPGWIATPLTKALQEDPARSGPILQRTPLNRWGTPEDIGNVAAFLCTPAASFMTGAVVP 184 VAPG+I T +T L E+ + ++ PL R+G+PE++ NV FLC+ AS++TG V+ Sbjct: 185 VAPGFIETDMTAVLSEEIKQKYK--EQIPLGRFGSPEEVANVVLFLCSELASYITGEVIH 242 Query: 185 VDGG 188 V+GG Sbjct: 243 VNGG 246