BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0213.p (295 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 142 7e-34 mll5767 Non COG 86 1e-16 PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TI... 75 2e-13 mlr1708 Non COG 73 1e-12 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 69 1e-11 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 142 bits (359), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 97/293 (33%), Positives = 140/293 (47%), Gaps = 20/293 (6%) Query: 3 MRIDAHQHFWRYRAEDYPWIGAGMDVLAQDKLPADLLPLLRAHQLDASIAVQARSGRDET 62 M IDAHQHFW + W + + +D LP DL PLL A + ++ VQ +T Sbjct: 15 MLIDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQTMETAADT 74 Query: 63 RFLLGLAQEHDFIAGVVGWENLAVPDLGERLDEWHGGDKLLGFRHQVQDEADAGAFLEHA 122 F+L LA+ DFI GVVGW ++ ++ + G R +Q D A+++ A Sbjct: 75 DFMLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMEDV-AWIDDA 133 Query: 123 DFNRGVALLQERHYVYDVLVFESQLADTQAFCARHDKHWLVLDHLGKPALREFGKNGGNS 182 N V L + V+D LV L +F ++H + +V+DH KP + G Sbjct: 134 ALNAAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLI----ATGHYR 189 Query: 183 DDALWRWEKELLALGALPHVVCKLSGLVTEADWQNGLQPADYQHIRICLDTALEAFGPQR 242 D W + + AL A P+V CKLSGL+TE Q + +R +T LE FG R Sbjct: 190 D-----WRRNMAALAARPNVHCKLSGLLTERGEQRP------EAVRPYAETILELFGGDR 238 Query: 243 LMFGSDWPVCLLADSYGKVADLIADWATSNLNKDEQDALWGGTAARCYGIKVR 295 ++FGSDWPV LA + D D + + A++GG A R Y + R Sbjct: 239 VIFGSDWPVLRLAGDFQGWLDFCRDIVPAK----DHAAVFGGNAIRFYSLTDR 287 >mll5767 Non COG Length = 297 Score = 85.9 bits (211), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 80/306 (26%), Positives = 126/306 (41%), Gaps = 37/306 (12%) Query: 5 IDAHQHFWRYRAEDYPWIGAGMDVLAQDKLPA--------DLLPLLRAHQLDASI----A 52 ID H H W YPW+ G+ A A D L R L S+ Sbjct: 7 IDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLDVG 66 Query: 53 VQARSGRDETRFLLGLAQEHDFIAGVVGWENLAVPDLGERLDEWHGGDKLLGFRHQVQDE 112 ETR+L +A + F G+VG+ +L+ PD+GE LD G R + Sbjct: 67 FDPSDPVGETRWLQEIANKRGFPHGIVGYADLSKPDVGELLDRHMEYPNFRGIRQSMNYH 126 Query: 113 ADAGAFLE-------HADFNRGVALLQERHYVYDVLVFESQLADTQAFCARHDKHWLVLD 165 AD + ++ RG L +R +D+ ++ Q+ + ++L+ Sbjct: 127 ADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQIILN 186 Query: 166 HLGKPALREFGKNGGNSDDALWRWEKELLALGALPHVVCKLSGL-VTEADWQNGLQPADY 224 H G ++ G N+ W+K + L P+V CK+SGL + + W Sbjct: 187 HTG---MQVDGPKHFNA------WKKGMHRLAQAPNVACKISGLGMGDWSWTTA------ 231 Query: 225 QHIRICLDTALEAFGPQRLMFGSDWPVCLLADSYGKVADLIADWATSNLNKDEQDALWGG 284 IR ++ A+ AFG R MF +++PV L S+ V + + T +E+ AL+ Sbjct: 232 -SIRPYVEEAIAAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE-ITRYYPHEERLALFHD 289 Query: 285 TAARCY 290 AAR Y Sbjct: 290 NAARHY 295 >PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Pae Pseudomonas aeruginosa Length = 319 Score = 75.1 bits (183), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 79/309 (25%), Positives = 129/309 (41%), Gaps = 42/309 (13%) Query: 5 IDAHQHFWRYRAEDYPWIGAGMDV---------LAQDKLPADLLPLLRAHQLDASIAVQA 55 +DAH HFW +A +PW+ + + + LPAD + AH++ ++ V+ Sbjct: 10 VDAHHHFWDPQANYHPWLAEDAKIPFRYGDYSAIKRRYLPADYFGDVGAHRVVETVYVET 69 Query: 56 ----RSGRDETRFLLGLAQEHDFIAGVVGWENLAVPDLGERLDEWHGGDKLLGFRHQ--- 108 R ETRF+ LA+ H VV L PD E L G ++ RH+ Sbjct: 70 EWDPRDPLGETRFVHHLAERHGAPHAVVAQAWLDAPDAAEVLAAQAGFARVRSVRHKPGG 129 Query: 109 ---VQDEADAGAFLEHADFNRGVALLQERHYVYDVLVFESQLADTQAFCARHDKHWLVLD 165 Q + + + + RG A L +D+ LA+ +VL+ Sbjct: 130 PQRPQQVGELRSLMSDERWRRGYAELARHGLHFDLQTPWWNLAEAACLARDFPDTLIVLN 189 Query: 166 HLGKPALREFGKNGGNSDDALWRWEKELLALGALPHVVCKLSGLVTEADWQNGLQPADYQ 225 H G P+ R S++ L W++ + P+V K+SG+ Q G + + Sbjct: 190 HAGLPSDR--------SEEGLAAWQRAMARFAECPNVALKISGIG-----QAGRRWSVED 236 Query: 226 HIRICLDTALEAFGPQRLMFGSDWPVCLLA----DSYGKVADLIADWATSNLNKDEQDAL 281 + I ++ + FG +R MF S++PV L D YG ++AD L +Q+ L Sbjct: 237 NAWIVRES-IALFGVERAMFASNFPVDSLCGSFDDIYGGFKRIVAD-----LPYADQERL 290 Query: 282 WGGTAARCY 290 + A R Y Sbjct: 291 FHSNARRIY 299 >mlr1708 Non COG Length = 295 Score = 72.8 bits (177), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 77/309 (24%), Positives = 124/309 (40%), Gaps = 36/309 (11%) Query: 1 MTMRIDAHQHFWRYRAEDYPWIGAGM--------DVLAQDKLPADLLPLLRAHQLDASIA 52 M IDAH H WR + D PW+ M + + +D + L + ++ ++ Sbjct: 1 MQKAIDAHFHIWRQK--DQPWLVGPMVPRIFGPYEPIRRDYPIGEFLQDQKGSGVEKAVY 58 Query: 53 VQARSGRD----ETRFLLGLAQEHDFIAGVVGWENLAVPDLGERLDEWHGGDKLLGFRHQ 108 VQ ++ E FL A E + +VG+ ++ V D+ ++D L G R Q Sbjct: 59 VQTNWAKEDFEKEVAFLQQTADETGWPHAIVGYADMTVDDVRPQIDRLMKYRLLRGVRMQ 118 Query: 109 VQ-DEADAGAFLEHAD------FNRGVALLQERHYVYDVLVFESQLADTQAFCARHDKHW 161 + E A F AD VA L++ +D+ +F +Q+ D + + Sbjct: 119 LHWHETPAFRFAASADQVIDPKVRANVARLKDYGLSFDLQLFPAQMKDGVTLVGENPETN 178 Query: 162 LVLDHLGKPALREFGKNGGNSDDALWRWEKELLALGALPHVVCKLSGLVTEADWQNGLQP 221 +L H G G + W+ L L A P+ KLSGL T + Sbjct: 179 FILTHAGMLT--------GMEPEVTETWKAGLRTLSAAPNFYAKLSGLGT------FVHR 224 Query: 222 ADYQHIRICLDTALEAFGPQRLMFGSDWPVCLLADSYGKVADLIADWATSNLNKDEQDAL 281 D I +D A++ G LMFGS++P+ L S+ ++ D + E D Sbjct: 225 NDPALIAYIVDNAIDILGADHLMFGSNFPIEKLWTSHAELIKAHRDAVARHGAAAEADIF 284 Query: 282 WGGTAARCY 290 W TA + Y Sbjct: 285 W-NTAEKVY 292 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 297 Score = 68.9 bits (167), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 79/298 (26%), Positives = 125/298 (41%), Gaps = 36/298 (12%) Query: 5 IDAHQHFWRYRAEDYPWIGAGMDVLAQDKLPADLLPLLRAHQLDASIAVQARSGRDETRF 64 ID HQH Y W G LA AD L+ + +I ++ +G D+ + Sbjct: 21 IDTHQHLILRGKLGYAWT-RGEPSLAGSFTRADYAALVEGSGVIGTIFME--TGVDDGDY 77 Query: 65 LLGLAQEHDFIAGVVGWENLAV--------PDLGERLDEWHGGD---KLLGFRHQVQDEA 113 E +AG+VG L + P+ + D W K++GFR +Q Sbjct: 78 ----RAEARLVAGMVGEGTLPMFGQIASCRPETDQGFDAWLEECRHLKVVGFRRILQVMP 133 Query: 114 DAGAFLEHADFNRGVALLQERHYVYDVLVFESQLADTQAFCARHDKHWLVLDHLGKPALR 173 D + E ++ R V + + + D+ QLA +LDH G P + Sbjct: 134 DDTSRAE--NYRRNVRKIGKAGWPVDLCFHSRQLAIAAELVRACPDVRFMLDHCGTPDI- 190 Query: 174 EFGKNGGNSDDALWRWEKELLALGALPHVVCKLSGLVTE-ADWQNGLQPADYQHIRICLD 232 GN A W + + L +LP++ KLSG+ A Q L+ +R ++ Sbjct: 191 -----AGNDFPA---WSERMADLASLPNLFVKLSGVTAYCAPGQVTLE-----SVRPYVE 237 Query: 233 TALEAFGPQRLMFGSDWPVCLLADSYGKVADLIADWATSNLNKDEQDALWGGTAARCY 290 T L+ FGP R+++G DWPV L + + ++ S L+ DEQ ++ G A R Y Sbjct: 238 TVLKLFGPARMVWGGDWPVVDLGSGLPEWIAMTREF-LSTLSPDEQASIGHGNARRFY 294