BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0027.p (372 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2819 [E] COG0263 Glutamate 5-kinase Rso Ralstonia solanacearum 526 e-149 PA4565 [E] COG0263 Glutamate 5-kinase Pae Pseudomonas aeruginosa 442 e-124 AGc5045 [E] COG0263 Glutamate 5-kinase Atu Agrobacterium tumefac... 294 2e-79 SMc03776 [E] COG0263 Glutamate 5-kinase Sme Sinorhizobium meliloti 289 8e-78 DR1827 [E] COG0263 Glutamate 5-kinase Dra Deinococcus radiodurans 288 2e-77 >RSc2819 [E] COG0263 Glutamate 5-kinase Rso Ralstonia solanacearum Length = 374 Score = 526 bits (1356), Expect = e-149, Method: Compositional matrix adjust. Identities = 280/372 (75%), Positives = 316/372 (84%) Query: 1 MHSVIQQAKRLIIKVGSSLVTNDGKGLDSAAIAMWAEQIAHLRTLGKEVVLVSSGAIAEG 60 + S+I A+RL++KVGSSLVT+DG+GLD AAIA WA QIA LR GKEVVLVSSGAIAEG Sbjct: 3 LRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEG 62 Query: 61 MQRLGFDRRPTGVHELQACAAVGQMGLAQIYETSFRAHQLGTAQVLLTHADLADRERYLN 120 MQRLG+ RRP +HELQA AAVGQMGLAQ+YE+ F H + TAQVLLTH DLADRERYLN Sbjct: 63 MQRLGWTRRPKEIHELQAAAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERYLN 122 Query: 121 ARSTLFTLLRFGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEADALIILTDQRGLYT 180 ARSTL TLL GVVPIINENDTVVTDEIKFGDNDTLGALV NLIE DALIILTDQRGLYT Sbjct: 123 ARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGLYT 182 Query: 181 ADPRRDPDAQFVHEAKAGDLALEAMAGGAGSGIGRGGMLTKILAAKRAATSGAHTVIAWG 240 ADPR+ PDA+FV EA+AG LEAMAGGAGS IG+GGMLTKI+AAKRAA SGAHTVIA G Sbjct: 183 ADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIASG 242 Query: 241 REESVLSRLAAGEAIGTQLTAQTAQLTARKQWMADHLQTAGRVMLDDGAVQKLTAEGKSL 300 RE VL+RLA GEAIGTQL A T ++ ARKQWM DHLQ GRV+LD GAV KLTA GKSL Sbjct: 243 READVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGKSL 302 Query: 301 LPIGVTEVAGEFGRGDVITCVDASGKPVARGISNYASSDARRIVRHPSSEILSILGFVEE 360 LPIGVTEV GEF RG+VI+CVD +G+ VARG++NY++++AR I R SSEI ++LG+V Sbjct: 303 LPIGVTEVQGEFARGEVISCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYVSA 362 Query: 361 SELIHRDNLVLL 372 +EL+HRDNLVLL Sbjct: 363 AELVHRDNLVLL 374 >PA4565 [E] COG0263 Glutamate 5-kinase Pae Pseudomonas aeruginosa Length = 372 Score = 442 bits (1137), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/372 (58%), Positives = 285/372 (76%) Query: 1 MHSVIQQAKRLIIKVGSSLVTNDGKGLDSAAIAMWAEQIAHLRTLGKEVVLVSSGAIAEG 60 M + A+R ++K+GS+L+T DG+GLD A+A+W EQ+ L G E+VLVSSGA+A G Sbjct: 1 MRDKVTGARRWVVKIGSALLTADGRGLDRNAMAVWVEQMVALHCAGIELVLVSSGAVAAG 60 Query: 61 MQRLGFDRRPTGVHELQACAAVGQMGLAQIYETSFRAHQLGTAQVLLTHADLADRERYLN 120 M RLG+ RP+ +HELQA A+VGQMGL Q +E+SF H L TAQVLLTH DL+DR+RYLN Sbjct: 61 MSRLGWVSRPSAMHELQAAASVGQMGLVQAWESSFALHGLQTAQVLLTHDDLSDRKRYLN 120 Query: 121 ARSTLFTLLRFGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEADALIILTDQRGLYT 180 ARSTL TL+ GVVP+INENDTVVTDEI+FGDNDTL ALVANL+EAD L+ILTD+ G++ Sbjct: 121 ARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD 180 Query: 181 ADPRRDPDAQFVHEAKAGDLALEAMAGGAGSGIGRGGMLTKILAAKRAATSGAHTVIAWG 240 ADPR +PDAQ ++EA+A D L+A+AGG+ +GRGGM TK+ AA+ AA SG HTVI G Sbjct: 181 ADPRNNPDAQLIYEARADDPQLDAVAGGSAGALGRGGMQTKLRAARLAARSGGHTVIVGG 240 Query: 241 REESVLSRLAAGEAIGTQLTAQTAQLTARKQWMADHLQTAGRVMLDDGAVQKLTAEGKSL 300 R E VL RL AGE +GT LT ++ ARKQW+A HLQ G ++LDDGAV+ ++ + KSL Sbjct: 241 RIERVLDRLRAGERLGTLLTPDRSRKAARKQWLAGHLQMRGTLVLDDGAVKAVSQDHKSL 300 Query: 301 LPIGVTEVAGEFGRGDVITCVDASGKPVARGISNYASSDARRIVRHPSSEILSILGFVEE 360 LP+GV V G F RG+++ CVD G+ VARG+ NY++ +A++I+ P+ I ++LG+V+ Sbjct: 301 LPVGVKAVQGSFRRGEMVVCVDQGGREVARGLVNYSALEAQKILGQPTDAIEALLGYVDG 360 Query: 361 SELIHRDNLVLL 372 EL+HRDNLVL+ Sbjct: 361 PELVHRDNLVLV 372 >AGc5045 [E] COG0263 Glutamate 5-kinase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 389 Score = 294 bits (752), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 167/362 (46%), Positives = 226/362 (62%), Gaps = 2/362 (0%) Query: 10 RLIIKVGSSLVTNDGKGLDSAAIAMWAEQIAHLRTLGKEVVLVSSGAIAEGMQRLGFDRR 69 R++IK+GS+L+ + GL + E IA L+ G +V +VSSGAIA G LG Sbjct: 13 RIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAIALGRTVLGLPSG 72 Query: 70 PTGVHELQACAAVGQMGLAQIYETSFRAHQLGTAQVLLTHADLADRERYLNARSTLFTLL 129 + E QA AAVGQ+ LA+ + S H++ Q+LLT +D +R RYLNAR+T+ LL Sbjct: 73 ALKLEESQAAAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRRYLNARATINQLL 132 Query: 130 RFGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEADALIILTDQRGLYTADPRRDPDA 189 + G +PIINENDTV T EI++GDND L A VA + AD L++L+D GLYTA P DPDA Sbjct: 133 KIGAIPIINENDTVATTEIRYGDNDRLAARVATMTGADLLVLLSDIDGLYTAPPHLDPDA 192 Query: 190 QFVHEAKAGDLALEAMAGGAGSGIGRGGMLTKILAAKRAATSGAHTVIAWGREESVLSRL 249 +F+ +EAMAGGA S + RGGM TKI A K A +G +IA G+ + L + Sbjct: 193 KFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMIIASGKTLNPLKAI 252 Query: 250 AAGEAIGTQLTAQTAQLTARKQWMADHLQTAGRVMLDDGAVQKLTAEGKSLLPIGVTEVA 309 G A + +TARK W+A LQ AG + +D GA + L A GKSLLP GV +V Sbjct: 253 ENG-ARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYA-GKSLLPAGVRQVK 310 Query: 310 GEFGRGDVITCVDASGKPVARGISNYASSDARRIVRHPSSEILSILGFVEESELIHRDNL 369 G FGRGD I + G+ VARG++ Y + +AR I+ H S+EI +ILG+V + +IHRD+L Sbjct: 311 GNFGRGDAIAIIGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVGRAAMIHRDDL 370 Query: 370 VL 371 V+ Sbjct: 371 VM 372 >SMc03776 [E] COG0263 Glutamate 5-kinase Sme Sinorhizobium meliloti Length = 393 Score = 289 bits (739), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 164/367 (44%), Positives = 229/367 (62%), Gaps = 2/367 (0%) Query: 5 IQQAKRLIIKVGSSLVTNDGKGLDSAAIAMWAEQIAHLRTLGKEVVLVSSGAIAEGMQRL 64 ++ +R++IK+GS+L+ + GL + + IA LR G EV++VSSGAIA G L Sbjct: 8 LENYRRVVIKIGSALLVDRRTGLKKSWLDALCADIAALRAKGVEVLVVSSGAIALGRTVL 67 Query: 65 GFDRRPTGVHELQACAAVGQMGLAQIYETSFRAHQLGTAQVLLTHADLADRERYLNARST 124 + E QA AAVGQ+ LA+ + S H + Q+LLT D +R RYLNAR+T Sbjct: 68 DLPAGALKLEESQAAAAVGQIVLARAWSESLSTHAIVAGQILLTLGDTEERRRYLNARAT 127 Query: 125 LFTLLRFGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEADALIILTDQRGLYTADPR 184 + LL+ G VPIINENDTV T EI++GDND L A VA ++ AD L++L+D GLYTA P Sbjct: 128 IGQLLKLGSVPIINENDTVATTEIRYGDNDRLAARVATMVGADLLVLLSDIDGLYTAPPH 187 Query: 185 RDPDAQFVHEAKAGDLALEAMAGGAGSGIGRGGMLTKILAAKRAATSGAHTVIAWGREES 244 DP+A+F+ +EAMAGGA S + RGGM TKI A K A T+G +IA G+ + Sbjct: 188 LDPNARFLETVAEITPEIEAMAGGAASELSRGGMRTKIDAGKIATTAGCAMIIASGKPDH 247 Query: 245 VLSRLAAGEAIGTQLTAQTAQLTARKQWMADHLQTAGRVMLDDGAVQKLTAEGKSLLPIG 304 L+ + AG A + + +TARK W+A L AG + +D GA L + GKSLLP G Sbjct: 248 PLAAIEAG-ARSSWFAPSGSPVTARKTWIAGQLLPAGSLSIDAGAETALRS-GKSLLPAG 305 Query: 305 VTEVAGEFGRGDVITCVDASGKPVARGISNYASSDARRIVRHPSSEILSILGFVEESELI 364 V +V G F RGD I + ASG+ +ARG++ Y + +AR+I S+EI +ILG+ + ++ Sbjct: 306 VRQVTGSFSRGDTIAIIGASGREIARGLAGYDADEARQIAGKKSAEIAAILGYAGRTAMV 365 Query: 365 HRDNLVL 371 HRD+LV+ Sbjct: 366 HRDDLVM 372 >DR1827 [E] COG0263 Glutamate 5-kinase Dra Deinococcus radiodurans Length = 363 Score = 288 bits (736), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 165/363 (45%), Positives = 223/363 (61%), Gaps = 1/363 (0%) Query: 10 RLIIKVGSSLVTNDGKGLDSAAIAMWAEQIAHLRTLGKEVVLVSSGAIAEGMQRLGFDRR 69 R+++K+G+S++T L + IA + G EVVLVSSGA+ G + LGF R Sbjct: 2 RVVLKLGTSVLTAGTDRLHRPRLVDLMRDIAAVSAQGHEVVLVSSGAVTAGWEALGFPPR 61 Query: 70 PTGVHELQACAAVGQMGLAQIYETSFRAHQLGTAQVLLTHADLADRERYLNARSTLFTLL 129 + E Q AAVGQ+ L +Y + + L +AQ+LLT D +R RYLNAR+TL L Sbjct: 62 ERTLAEKQLLAAVGQVQLMHLYTSLAELYGLRSAQLLLTADDFRERTRYLNARTTLEGCL 121 Query: 130 RFGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEADALIILTDQRGLYTADPRRDPDA 189 GV+P+INENDTV ++IK GDNDTL A VANL+EAD L+ILTD GLYTADPR PDA Sbjct: 122 SRGVLPVINENDTVAVEQIKVGDNDTLSAFVANLVEADLLLILTDAPGLYTADPRTHPDA 181 Query: 190 QFVHEAKAGDLALEAMAGGAGSGIGRGGMLTKILAAKRAATSGAHTVIAWGREESVLSRL 249 + + + A+AGGAGS G GGM TKI AA+ A +G VIA G L R+ Sbjct: 182 TLIPVVERVTPDIWALAGGAGSHRGTGGMHTKIQAAEIATRAGTPVVIAPGDLPEALRRV 241 Query: 250 AAGEAIGTQLTAQTAQLTARKQWMADHLQTAGRVMLDDGAVQKLTAEGKSLLPIGVTEVA 309 GEA+GT+ A +L ARK+W+ + GR++LD GA Q + G SLLP G+ +V Sbjct: 242 VDGEALGTRFLAHGTRLEARKRWILAEI-AHGRLLLDGGAAQAVRERGSSLLPAGIRQVE 300 Query: 310 GEFGRGDVITCVDASGKPVARGISNYASSDARRIVRHPSSEILSILGFVEESELIHRDNL 369 G+F RG + + G+ + RG++ Y + D RR+ H S EI ++LG+ E +HRD+L Sbjct: 301 GDFERGHTVRLLAPDGQELGRGLTRYRADDLRRVCGHHSREIEALLGYTYGEEAVHRDDL 360 Query: 370 VLL 372 VLL Sbjct: 361 VLL 363