BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0038.p (264 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1419 [J] COG0566 rRNA methylases Rso Ralstonia solanacearum 246 4e-65 NMA1351 [J] COG0566 rRNA methylases NmA Neisseria meningitidis Z... 168 1e-41 NMB1179 [J] COG0566 rRNA methylases Nme Neisseria meningitidis MC58 130 3e-30 NMB1141 [J] COG0566 rRNA methylases Nme Neisseria meningitidis MC58 130 3e-30 CAC2358 [J] COG0566 rRNA methylases Cac Clostridium acetobutylicum 126 4e-29 >RSc1419 [J] COG0566 rRNA methylases Rso Ralstonia solanacearum Length = 259 Score = 246 bits (627), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 126/262 (48%), Positives = 173/262 (66%), Gaps = 3/262 (1%) Query: 1 MKTISSRDNPLYKELKQLATSAQARRKAGRSLLDGVHLAQAYLQGAGKPAFCIVAESALH 60 MK I+SRDN ++K LK L+ S Q RR+AG+S+LDG+HL AYL PA C+V+E L Sbjct: 1 MKHITSRDNAVFKHLKALSGSTQQRRRAGQSVLDGIHLVAAYLDAGHVPAQCLVSERHLA 60 Query: 61 HAEVAPVLAQCEAAGSQCICLTDALYQAASQVENGVGLLLVIATPQPSAPTALTTSAVLL 120 H EVAP+LA+ + G + L D+L+ + V NG+ L+ +I TP+ P + V+L Sbjct: 61 HPEVAPLLARVDPQG--IVLLADSLFAQLTTVVNGIDLMALIETPEGHLPHRIEHDCVIL 118 Query: 121 DGLQDPGNLGSILRSAAAAGIGQVFCSAGTAAAWSPKVLRAGMGAHFQLEIFENADLLSL 180 DG+QD GN+GSILR AAAAG+ VF + G A AWS K LRA MGAHF L + E+ S+ Sbjct: 119 DGIQDAGNVGSILRCAAAAGVRDVFMTTGCAFAWSAKTLRAAMGAHFHLNVVEHCTFESV 178 Query: 181 CKGSAIPVLATSSYAQKNIYQTDLTQAVAWLFGHEGQGVADDLLDAASDKVVIPHLGEME 240 +P+LATSS+A+ ++ L + V W+FG+EG GV++ L + + + IP G ME Sbjct: 179 HARLHVPLLATSSHARHAVFDMPLNKPVGWVFGNEGAGVSEQWL-SVTTPISIPQPGGME 237 Query: 241 SLNVAAAAAVCFFEQVRQKQGN 262 SLNVAAAAA+C FE VRQ++ N Sbjct: 238 SLNVAAAAAICLFEAVRQRRMN 259 >NMA1351 [J] COG0566 rRNA methylases NmA Neisseria meningitidis Z2491 Length = 261 Score = 168 bits (425), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 113/264 (42%), Positives = 151/264 (57%), Gaps = 11/264 (4%) Query: 1 MKTISSRDNPLYKELKQLATSAQARRKAGRSLLDGVHLAQAYLQGAGKPAFCIVAESALH 60 MK ISS +N + L +L + + RR+ +++L+GVHL Q +LQ G P + E+ + Sbjct: 1 MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQSGGMPVGVYIPEAKMP 60 Query: 61 HAEVAPVLAQCEAAGSQCICLTDALYQAASQVENGVGLLLVIATPQPSAPTALTTSAVLL 120 EV + A G ++D + + S + +L +I P A A V+L Sbjct: 61 SEEVRKLTAVLPEDG--FFSVSDGILKKISSLTCADDVLALIDIPDAGALPA-GGDCVVL 117 Query: 121 DGLQDPGNLGSILRSAAAAGIGQVFCSAGTAAAWSPKVLRAGMGAHFQLEIFENADL--- 177 DG+QDPGN+G++LRSAAAAGIG V G A AWSPKVLRAGMGAHF EI+ ADL Sbjct: 118 DGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGMGAHFLSEIYPQADLEIW 177 Query: 178 LSLCKGSAIPVLATSSYAQKN--IYQTDLTQAVAWLFGHEGQGVADDLLDAASDKVVIPH 235 L KG V AT+ +K +Y DL + AW+FG+EG GV +LD A V IP Sbjct: 178 LVRYKGR---VFATALREEKQAVLYGEDLCEPTAWVFGNEGAGVGKAVLDRADKCVRIPM 234 Query: 236 LGEMESLNVAAAAAVCFFEQVRQK 259 ESLNVA AA +C FEQ+RQ+ Sbjct: 235 HDATESLNVAMAATICLFEQMRQR 258 >NMB1179 [J] COG0566 rRNA methylases Nme Neisseria meningitidis MC58 Length = 203 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 88/184 (47%), Positives = 111/184 (60%), Gaps = 9/184 (4%) Query: 81 LTDALYQAASQVENGVGLLLVIATPQPSAPTALTTSAVLLDGLQDPGNLGSILRSAAAAG 140 ++D + + S + +L +I P A A V+LDG+QDPGN+G++LRSAAAAG Sbjct: 21 VSDGILKKISSLTCADDVLALIDIPDAGALPA-GGDCVVLDGVQDPGNVGTVLRSAAAAG 79 Query: 141 IGQVFCSAGTAAAWSPKVLRAGMGAHFQLEIFENADL---LSLCKGSAIPVLATSSYAQK 197 IG V G A AWSPKVLRAGMGAHF EI+ ADL L KG V AT+ +K Sbjct: 80 IGAVILGKGCADAWSPKVLRAGMGAHFLSEIYPQADLEIWLVRYKGR---VFATALREEK 136 Query: 198 N--IYQTDLTQAVAWLFGHEGQGVADDLLDAASDKVVIPHLGEMESLNVAAAAAVCFFEQ 255 +Y DL + AW+FG+EG GV +LD A V IP ESLNVA AA +C FEQ Sbjct: 137 QAVLYGEDLCEPTAWVFGNEGAGVGKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQ 196 Query: 256 VRQK 259 +RQ+ Sbjct: 197 MRQR 200 >NMB1141 [J] COG0566 rRNA methylases Nme Neisseria meningitidis MC58 Length = 203 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 88/184 (47%), Positives = 111/184 (60%), Gaps = 9/184 (4%) Query: 81 LTDALYQAASQVENGVGLLLVIATPQPSAPTALTTSAVLLDGLQDPGNLGSILRSAAAAG 140 ++D + + S + +L +I P A A V+LDG+QDPGN+G++LRSAAAAG Sbjct: 21 VSDGILKKISSLTCADDVLALIDIPDAGALPA-GGDCVVLDGVQDPGNVGTVLRSAAAAG 79 Query: 141 IGQVFCSAGTAAAWSPKVLRAGMGAHFQLEIFENADL---LSLCKGSAIPVLATSSYAQK 197 IG V G A AWSPKVLRAGMGAHF EI+ ADL L KG V AT+ +K Sbjct: 80 IGAVILGKGCADAWSPKVLRAGMGAHFLSEIYPQADLEIWLVRYKGR---VFATALREEK 136 Query: 198 N--IYQTDLTQAVAWLFGHEGQGVADDLLDAASDKVVIPHLGEMESLNVAAAAAVCFFEQ 255 +Y DL + AW+FG+EG GV +LD A V IP ESLNVA AA +C FEQ Sbjct: 137 QAVLYGEDLCEPTAWVFGNEGAGVGKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQ 196 Query: 256 VRQK 259 +RQ+ Sbjct: 197 MRQR 200 >CAC2358 [J] COG0566 rRNA methylases Cac Clostridium acetobutylicum Length = 261 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 78/260 (30%), Positives = 138/260 (53%), Gaps = 10/260 (3%) Query: 4 ISSRDNPLYKELKQLATSAQARRKAGRSLL-DGVHLAQAYLQGAGKPAFCIVAESALHHA 62 I S++N + KE+K+L + + R +S + +G+ + L+ K + V+E + Sbjct: 5 IKSKENSIIKEVKKL--NEKKHRDLKKSFIVEGLRFVREALESDFKIQYLFVSEKN-YDK 61 Query: 63 EVAPVLAQCEAAGSQCICLTDALYQAASQVENGVGLLLVIATPQPSAPTALTTS-AVLLD 121 + L + Q I +T+++ + + G++ V+ + LT +L+D Sbjct: 62 FILKELKELIKKEFQVIKVTESILKDICNTQTPQGIVAVVQYKE--VAWKLTKGFYMLVD 119 Query: 122 GLQDPGNLGSILRSAAAAGIGQVFCSAGTAAAWSPKVLRAGMGAHFQLEI-FENADL--L 178 +QDPGN+G+I+R+A AAG V + GT ++ K LRA MG+ F++ I FE+ + + Sbjct: 120 KVQDPGNMGTIIRTANAAGALGVIITKGTVDIYNNKTLRATMGSIFKIPIIFEDDNFSKI 179 Query: 179 SLCKGSAIPVLATSSYAQKNIYQTDLTQAVAWLFGHEGQGVADDLLDAASDKVVIPHLGE 238 K + ++A+S + N Y L + + G+EG G++D ++D + KV IP G Sbjct: 180 KELKKNGFKIIASSLDTEHNFYDVSLNEKLIIAVGNEGNGISDKIVDISDTKVKIPMPGN 239 Query: 239 MESLNVAAAAAVCFFEQVRQ 258 +ESLNV AAA+ FE VR+ Sbjct: 240 VESLNVGVAAAIMMFEYVRK 259