BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0014.p (480 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 491 e-138 SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene te... 479 e-135 mlr7299 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 445 e-124 AGl2922 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 439 e-123 mll9123 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 433 e-121 >BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 441 Score = 491 bits (1263), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/415 (57%), Positives = 309/415 (74%), Gaps = 6/415 (1%) Query: 53 AQPDAGQNIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLTSRAATFEPLTLLSA 112 A DA N+ +++++A+TAER K D LFLAD ++I S + LT FEP TLLSA Sbjct: 29 APSDASMNLDYFKELAKTAERGKLDMLFLADSLSIDSKSHPNV-LTR----FEPFTLLSA 83 Query: 113 LSQATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNLDKH 172 L+Q T IGL AT STTY+EP+H+AR+FASLDHLSNGRAGWNVVTS ES A NF+ +KH Sbjct: 84 LAQVTSKIGLTATASTTYSEPFHIARQFASLDHLSNGRAGWNVVTSSIESTALNFSGEKH 143 Query: 173 PEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVRGPL 232 EH RY+RAEEFV++V+GLWDSWE+DAF ++ G FD K+H ++HKG++FSVRGPL Sbjct: 144 LEHHLRYQRAEEFVEIVKGLWDSWEEDAFIRNKETGEFFDKEKMHELNHKGEYFSVRGPL 203 Query: 233 NVARPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYGREP 292 NV+R QG PV++QAGSS G+ LAA+TAEVIFTAQ LE AQ FY S+K + A +GR+P Sbjct: 204 NVSRTPQGQPVIIQAGSSGDGKALAAKTAEVIFTAQNHLESAQEFYQSIKEQAAEFGRDP 263 Query: 293 DELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGIDLSGYPLDGPLPE 352 +++ IMPG+FP++ +E A K++ELQ LI P+VGL +LQ +LGGIDLS YPLDGPLP+ Sbjct: 264 EKIAIMPGIFPIIADTEEAAQAKYKELQDLIIPSVGLQILQNYLGGIDLSAYPLDGPLPK 323 Query: 353 -NLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPESIVDRLEEWFLK 411 + + N KSRF+LV +A R+ +TIR+LY VA +RGH GTPE + D+++EW Sbjct: 324 LDAEASNAVKSRFKLVQEMAERDNMTIRELYKYVAGSRGHHIFVGTPEQLADKMQEWVDT 383 Query: 412 GGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENLGLARPAHR 466 DGFNIMPP LP G++ FV+ V+P L+ RG+FR EYEG TLRE+ GL +P +R Sbjct: 384 KACDGFNIMPPLLPEGIEVFVDQVVPILQERGVFRKEYEGTTLREHFGLEKPVNR 438 >SMb21691 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Sme Sinorhizobium meliloti Length = 449 Score = 479 bits (1233), Expect = e-135, Method: Compositional matrix adjust. Identities = 247/428 (57%), Positives = 305/428 (71%), Gaps = 6/428 (1%) Query: 41 PDAGQNIAHYXR--AQPDAGQNIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLT 98 P GQ+IA + A D N +R++A+TAER FDA FLAD +++ E + Sbjct: 12 PGGGQHIAAWRHPDAPADGATNFEFHRRLAETAERGLFDAYFLADNLSVGLGGREGGN-- 69 Query: 99 SRAATFEPLTLLSALSQATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTS 158 ++ A FEP+TL +AL+ T H+G +AT STTY EPY +ARKFASLD LSNGRAGWNVVTS Sbjct: 70 AKIAGFEPVTLFAALAPLTTHLGFIATASTTYEEPYTLARKFASLDLLSNGRAGWNVVTS 129 Query: 159 WSESEAHNFNLDKHPEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHA 218 + A NFN + P H RYERA E V+ V+ LWDSWEDDAF D+A G FD ++H Sbjct: 130 AGDETARNFNRETQPSHADRYERAHEHVETVKALWDSWEDDAFIRDKATGRFFDAGRVHD 189 Query: 219 IHHKGKHFSVRGPLNVARPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFY 278 I HKGKHFSV+GPLN RPVQGHPVVVQAG SE G+ LAA +AEVIFTA Q+L AQ FY Sbjct: 190 IDHKGKHFSVKGPLNAPRPVQGHPVVVQAGQSEDGRRLAAVSAEVIFTAHQNLASAQEFY 249 Query: 279 SSLKSRLARYGREPDELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGG 338 +K+R+ R GR P+ + IMPGV P VGR+E EA K+EEL +LI P G++LL GG Sbjct: 250 RDIKARVKRAGRNPEHVLIMPGVAPFVGRTEEEARSKYEELNALIVPEDGVALLNGLTGG 309 Query: 339 -IDLSGYPLDGPLPENLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGT 397 +DL+GYPLDGPLP + E G KSR L+ +A TIRQLY +ATARGH+++ G+ Sbjct: 310 TLDLTGYPLDGPLPVS-NETEGMKSRQALIRKIADEHGFTIRQLYQWIATARGHYTVVGS 368 Query: 398 PESIVDRLEEWFLKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLREN 457 E + D+LEEWFL ADGFNI+PP LPG LDDFV+LV+P L+RRGLFR+ YEG+TLREN Sbjct: 369 AEQVADQLEEWFLSEAADGFNILPPWLPGALDDFVDLVIPILQRRGLFRTAYEGRTLREN 428 Query: 458 LGLARPAH 465 LGL RPA+ Sbjct: 429 LGLPRPAN 436 >mlr7299 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 451 Score = 445 bits (1145), Expect = e-124, Method: Compositional matrix adjust. Identities = 228/426 (53%), Positives = 292/426 (68%), Gaps = 3/426 (0%) Query: 50 YXRAQPDAGQNIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLTSRAATFEPLTL 109 Y A PDA N AH ++ AQT E AKFDA F+AD +A+ + E L + +FEP TL Sbjct: 25 YPGAYPDANFNFAHLKRFAQTLEAAKFDAFFMADHLAVLNMPIEALRRSHTVTSFEPFTL 84 Query: 110 LSALSQATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNL 169 LSAL+ T+HIGLVAT STT++ PYH+AR+FASLDH+S GRAGWN+VT+ + A NF L Sbjct: 85 LSALAAVTDHIGLVATASTTFDAPYHIARRFASLDHISGGRAGWNIVTTSNPDAALNFGL 144 Query: 170 DKHPEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVR 229 D+H EH RY RA EF DVV GLWDS+ DDAF D G++FD KLH + HKG+H SVR Sbjct: 145 DEHVEHDERYHRAREFYDVVTGLWDSFADDAFIRDAESGLYFDPAKLHVLDHKGEHLSVR 204 Query: 230 GPLNVARPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYG 289 GPLN+ARPVQG PV+VQAG+SE G++LAA TAEVIF A L QHF++ +K R + G Sbjct: 205 GPLNIARPVQGWPVIVQAGASEAGRQLAAETAEVIFAAHADLAAGQHFFADVKGRAQKLG 264 Query: 290 REPDELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGIDLSGYPLDGP 349 R D++KI+PG F VVG S +A K +L SL++ G++ L LG + S + D Sbjct: 265 RSRDDIKILPGAFVVVGDSVEDARAKRAKLDSLVYYESGIASLSIALGH-NASAFDPDAA 323 Query: 350 LPENLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPESIVDRLEEWF 409 LPE + E N +KS + V LAR E LT+RQL R+ G + GTP+ I D +E+W Sbjct: 324 LPE-IPETNASKSSRERVIELARNENLTVRQLAQRLGGYSG-LAFVGTPQIIADEMEKWL 381 Query: 410 LKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENLGLARPAHRIYQ 469 + G+DGFNIM P LP GLDDFVE V+PEL+RRG+FR +YEG TLRENLGL RP +R ++ Sbjct: 382 VTEGSDGFNIMFPYLPAGLDDFVEKVVPELQRRGIFRRQYEGSTLRENLGLKRPPNRFFE 441 Query: 470 QSGAGV 475 + + V Sbjct: 442 DAKSAV 447 >AGl2922 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 436 Score = 439 bits (1130), Expect = e-123, Method: Compositional matrix adjust. Identities = 222/427 (51%), Positives = 302/427 (70%), Gaps = 13/427 (3%) Query: 43 AGQNIAHYXRAQPDAG-QNIAHYRQIAQTAERAKFDALFLADGV--AIRSHDAETLHLTS 99 AG +++ + +AG +N ++ + AE AKFD +FLADG+ + +H S Sbjct: 19 AGHHVSGWRHPDAEAGSENFDLLTRVTKMAEAAKFDMVFLADGLTSGVDAH-------PS 71 Query: 100 RAATFEPLTLLSALSQATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSW 159 A FEPLTLL+AL+ T+ IGL AT STTY EPYH AR FAS+DHLS+GRA WN+VT+ Sbjct: 72 MIARFEPLTLLAALAMVTDKIGLAATASTTYGEPYHTARAFASIDHLSHGRAAWNIVTTS 131 Query: 160 SESEAHNFNLDKHPEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAI 219 A NF+ HPEH RY AEE+V+VVRGLWDSW+DDAF D+ G + D K+H + Sbjct: 132 YARTAANFS-KSHPEHDERYAVAEEYVNVVRGLWDSWDDDAFVKDKQAGRYVDPEKVHIL 190 Query: 220 HHKGKHFSVRGPLNVARPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYS 279 H+GK+F+V+GPLN+ R QGHPV++QAGSS PGQ+LAARTA+++FTAQQ++ +AQ FY+ Sbjct: 191 DHEGKYFTVKGPLNIPRSPQGHPVLIQAGSSGPGQDLAARTADIVFTAQQAISEAQAFYT 250 Query: 280 SLKSRLARYGREPDELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGI 339 SLK+R+A++GR+P + +MPG P++GRS EA EK +EL + LL++ +G Sbjct: 251 SLKARVAKFGRDPASVAVMPGFMPIIGRSFEEAGEKLKELNRWTDIKNAMPLLEERIGH- 309 Query: 340 DLSGYPLDGPLPENLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPE 399 L+ Y LDGPLP +L + +SR +L+T LARRE+LTIR+L LRVA RGH + GT + Sbjct: 310 SLADYDLDGPLP-DLPISDQLRSRAELLTELARREKLTIRELALRVAAGRGHHIVMGTAK 368 Query: 400 SIVDRLEEWFLKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENLG 459 + DR+++WF G ADGFN+MPP PGGL++F V+P L+ RGLFR +YEG TLR++LG Sbjct: 369 EVADRMQQWFENGAADGFNVMPPFFPGGLEEFNREVVPLLQERGLFRKDYEGSTLRDHLG 428 Query: 460 LARPAHR 466 +ARPA R Sbjct: 429 IARPAVR 435 >mll9123 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 455 Score = 433 bits (1114), Expect = e-121, Method: Compositional matrix adjust. Identities = 217/424 (51%), Positives = 285/424 (67%), Gaps = 1/424 (0%) Query: 50 YXRAQPDAGQNIAHYRQIAQTAERAKFDALFLADGVAIRSHDAETLHLTSRAATFEPLTL 109 + A DAG NI Y+ AE A +F+AD +R E + + R FEP TL Sbjct: 24 HMNAHSDAGANIERYKSYTTKAEAAALHFVFVADTAGVRESTVEAMSRSGRVVGFEPTTL 83 Query: 110 LSALSQATEHIGLVATVSTTYNEPYHVARKFASLDHLSNGRAGWNVVTSWSESEAHNFNL 169 L+AL+ T+ IGLVAT STT+NEPY++AR FASLDHLS GR GWNVVTS + EA NFNL Sbjct: 84 LAALAMTTKKIGLVATASTTFNEPYNLARVFASLDHLSCGRVGWNVVTSANPFEAQNFNL 143 Query: 170 DKHPEHGARYERAEEFVDVVRGLWDSWEDDAFRFDQAEGVHFDHNKLHAIHHKGKHFSVR 229 + P H RYERA EF +VV GLWDSW DDAF + +G+ FD +K++ ++H GK+F+ R Sbjct: 144 RELPPHDERYERAAEFAEVVEGLWDSWADDAFVKQRDDGIFFDPHKVNFLNHVGKYFATR 203 Query: 230 GPLNVARPVQGHPVVVQAGSSEPGQELAARTAEVIFTAQQSLEDAQHFYSSLKSRLARYG 289 GPLN++R QG+PV+ QAGSS G A R E+IFTAQQS+EDA+ FY S+K +G Sbjct: 204 GPLNISRSPQGYPVIAQAGSSPTGTWFAGRYGELIFTAQQSIEDAKEFYKSVKGFARDFG 263 Query: 290 REPDELKIMPGVFPVVGRSESEALEKFEELQSLIHPTVGLSLLQQHLGGI-DLSGYPLDG 348 R +L +MPG+ P+V + EA EK + LQSLIH + + L ++ LG + D+ LD Sbjct: 264 RWDADLLVMPGIVPIVAPTTQEAEEKRQALQSLIHEDIAVELAKRLLGYVMDVDKLSLDE 323 Query: 349 PLPENLQEPNGTKSRFQLVTALARREQLTIRQLYLRVATARGHWSIHGTPESIVDRLEEW 408 P+P++L E N +SR +L+ AR ++LT+RQL +A RGH G+P IVD++E++ Sbjct: 324 PVPDDLGETNNMQSRQKLLLDTAREKRLTVRQLAASLAIGRGHLVCVGSPSEIVDQMEQF 383 Query: 409 FLKGGADGFNIMPPTLPGGLDDFVELVLPELRRRGLFRSEYEGKTLRENLGLARPAHRIY 468 +G ADGF IMPPT P GLDDFV+L++PELRRR LFRSEY G TLRENLGL RP ++ Sbjct: 384 HTEGAADGFIIMPPTFPDGLDDFVQLIIPELRRRKLFRSEYNGATLRENLGLRRPGNKRT 443 Query: 469 QQSG 472 Q G Sbjct: 444 DQMG 447