BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0228.p (267 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 266 1e-72 gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbas... 97 1e-21 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 89 3e-19 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 80 2e-16 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 77 1e-15 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 266 bits (680), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 140/266 (52%), Positives = 176/266 (66%), Gaps = 1/266 (0%) Query: 1 MSNP-TSNKPGTALITGASTGIGATYADRLARRGYDLILVARDVKRLETLAARLRKETSV 59 M+ P T GTAL+TGAS+GIGA YADRLAR GYDLILVAR+ RL TLA R+ +T Sbjct: 1 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR 60 Query: 60 KVEILPADLVNKADLLKVEQRLRSDASVTMLLNNAGMSISGELADGDIEQYDAMIQLNIT 119 VE+L ADL A L +VE++LRSDAS+T+L+NNAG+ L D+E MI LN+T Sbjct: 61 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVEHMTGMIALNVT 120 Query: 120 AVLRLAAAIAPGFIARKGGTLINVASVLALAPELFNGAYSGTKAFVLNLSQSLQKELGNH 179 A RL A PGF+AR G +IN++S++ +APE+ NG Y G+KAFVL SQSL EL Sbjct: 121 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 Query: 180 GVRVQAVLPGATRTEIWERSGTAIENLPAEMLMEVDDLVDAALAGLDLGEQVTIPSLPDV 239 GV+VQAVLPGAT T+ W G +E+L ++M + +VDAAL G E VTIPSL + Sbjct: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRRELVTIPSLHEE 240 Query: 240 GQWEALTAARHAMAPNLSLSKPAARY 265 W AAR A+ LS + PA RY Sbjct: 241 DAWTGYEAARQALIARLSANTPAPRY 266 >gi|300310398|ref|YP_003774490.1| ribitol 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 243 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 60/178 (33%), Positives = 95/178 (53%), Gaps = 3/178 (1%) Query: 12 ALITGASTGIGATYADRLARRGYDLILVARDVKRLETLAARLRKETSVKVEILPADLVNK 71 A+ITGA++GIG ++L G +++V + K L+ LAARL + +V L L + Sbjct: 10 AVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQVIPQVTNL---LDAE 66 Query: 72 ADLLKVEQRLRSDASVTMLLNNAGMSISGELADGDIEQYDAMIQLNITAVLRLAAAIAPG 131 + + L+ + +L NAG I GEL D E D M+ LN+ AV++ A+AP Sbjct: 67 SCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVHAVAPH 126 Query: 132 FIARKGGTLINVASVLALAPELFNGAYSGTKAFVLNLSQSLQKELGNHGVRVQAVLPG 189 + RK G +I SV P + Y+G+K + Q++++++ HGVRV V PG Sbjct: 127 MMERKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQVSPG 184 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 74/206 (35%), Positives = 110/206 (53%), Gaps = 16/206 (7%) Query: 1 MSNPTSNKPGTALITGASTGIGATYADRLARRGYDLILVARDVKRLETLAARLR----KE 56 MSN +K +ITGAS+G+G T A LA G L+L AR +RLE L A + + Sbjct: 1 MSNNIKDK--VVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQA 58 Query: 57 TSVKVEILPADLVNKADLLKVEQRLRSDASVTMLLNNAGMSISGELADGDIEQYDAMIQL 116 +V ++ D V +A + K EQ + +L+NNAG+ +A +E++D MI + Sbjct: 59 IAVTTDVARRDDV-EALVAKGEQHF---GRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDV 114 Query: 117 NITAVLRLAAAIAPGFIARKGGTLINVASVLALAPELFNG---AYSGTKAFVLNLSQSLQ 173 N+ VL AA P F A+ G +INV+SV + ++F G YS TK V L++ L+ Sbjct: 115 NVKGVLYGVAAALPRFAAQSSGHIINVSSVAGI--KVFAGMGTVYSATKFAVRALTEGLR 172 Query: 174 KELGNHGVRVQAVLPGATRTEIWERS 199 E + GVR + PGA +E+ S Sbjct: 173 TEAPD-GVRTTIISPGAVDSELKTHS 197 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 63/225 (28%), Positives = 110/225 (48%), Gaps = 21/225 (9%) Query: 12 ALITGASTGIGATYADRLARRGYDLILVARDVKRLETLAARLRKETSVKVEILPADLVNK 71 ALITG+S GIG A +A+ G ++L RD +L +AA+LR E + D + Sbjct: 22 ALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHT-IHESSFDATSS 80 Query: 72 ADLLKVEQRLRSD-ASVTMLLNNAGMSISGELADGDIEQYDAMIQLNITAVLRLAAAIAP 130 ++ Q++ S+ + +L+NNAG+ L + + +I N+ +V A A+A Sbjct: 81 EEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAAQAVAR 140 Query: 131 GFIARKGGTLINVASVLA------LAPELFNGAYSGTKAFVLNLSQSLQKELGNHGVRVQ 184 I R G +IN+ SV + +AP Y+ TK V ++ + + G +G++V Sbjct: 141 HMIPRGAGKIINICSVQSELGRPGIAP------YTATKGAVKMFTKGMAIDWGKYGIQVN 194 Query: 185 AVLPGATRTEI-------WERSGTAIENLPAEMLMEVDDLVDAAL 222 + PG +TE+ + + + P+ +V+DLV AA+ Sbjct: 195 GLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAV 239 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 77.0 bits (188), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 58/182 (31%), Positives = 91/182 (50%), Gaps = 11/182 (6%) Query: 13 LITGASTGIGATYADRLARRGYDLILVARDVKRLETLAARLRKETSVKVEILPADLVNKA 72 LITGA+ G GA A + A G+ +I R +RL+ L L + KV L D+ +K+ Sbjct: 4 LITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD---KVLPLVLDVTSKS 60 Query: 73 DLLKVEQRLRSDAS----VTMLLNNAGMSIS-GELADGDIEQYDAMIQLNITAVLRLAAA 127 +E L S V +L+NNAG+++ G + + ++ MI N+ ++ + A Sbjct: 61 ---SIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHA 117 Query: 128 IAPGFIARKGGTLINVASVLALAPELFNGAYSGTKAFVLNLSQSLQKELGNHGVRVQAVL 187 + P + R GT+IN+ SV P Y TKAFV + +L+ +L GVR + Sbjct: 118 LLPDMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLA 177 Query: 188 PG 189 PG Sbjct: 178 PG 179 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 772,378 Number of extensions: 28625 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 45 Length of query: 267 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 183 Effective length of database: 1,222,130 Effective search space: 223649790 Effective search space used: 223649790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)