BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0225.p (579 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 597 e-171 gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 112 1e-25 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 56 7e-09 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 597 bits (1538), Expect = e-171, Method: Compositional matrix adjust. Identities = 356/574 (62%), Positives = 427/574 (74%), Gaps = 6/574 (1%) Query: 3 RHQRDFGLDRRVLFLAGIAVIIGGISTVAAFVLLNLIHFFTNLFFFQTFSLADRSPATHT 62 +H+RD+ DRR+ +L IA+ IG +ST AA++LL LI FTNLF+FQ SLA +PATHT Sbjct: 2 KHKRDYTADRRLPYLCAIALPIGLLSTGAAWLLLKLIALFTNLFYFQRLSLAASTPATHT 61 Query: 63 MGIVVVIVPVIGGLIVGLMARFGSDKIRGHGIPEAIEAILFGKSKMSGKVAVLKPLASGI 122 +G + ++P+ GGLIVGLMAR+GSDKIRGHGIPEAIEAILFGKS+MS KVA+LKPL+SGI Sbjct: 62 LGWIAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSGI 121 Query: 123 VIGSGGPFGAEGPIIMTGGAIGSLIAQHFHVSAAERKTLLVAGATAGMTAVFGTPVAAVL 182 VIGSGGPFGAEGPIIMTGG+IGSL+AQ H++AAERKTLLVAGA AGMTA+FGTP+AAVL Sbjct: 122 VIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAVL 181 Query: 183 LAVELLLFEWRPRSLLPVILACAVAGFARPLLLEPGPLFPLHTDPVGLSALLVCIVAGLL 242 LAVELLLFE +PRSLLPV LACAVAGF RP L+E GPLFPL ALL C+VAGL Sbjct: 182 LAVELLLFELKPRSLLPVALACAVAGFVRPWLVEAGPLFPLDVAAPTPVALLSCLVAGLA 241 Query: 243 SGALSSAMSMALYWIEDRFHALPLHWMWWPALGAVAVGIGGYFEPRALGVGYDVIADLLN 302 SG L++A+++ALY IED FH L LHWMWWPALG +AVG+GG +PRALGVGYDVI DLL+ Sbjct: 242 SGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDVIGDLLH 301 Query: 303 HHLAMSAVLALLVVKAIIWVIALGSGTSGGVLAPLLMLGAGLGTAIAYVVPGSDPALWPL 362 H+ +SA LALL+VKA+IW++ALGSGTSGGVLAPLLMLGAGLG ++ +PG LWPL Sbjct: 302 QHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGGSDGLWPL 361 Query: 363 VCMAAVLSGVLGAPLTAAVFALGLTGDFNAFLPLLLATGISYGFTVLVMRRSIMTEKIAR 422 VCMA VL VLGAPLTA VFA GLT A LPL+ T ++YG VL+M+RSIMTEKIAR Sbjct: 362 VCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYGVNVLLMKRSIMTEKIAR 421 Query: 423 RGLHIYREYSVDPQERMFVEEVMTREPVTISASLSLEEIATVYFGATQQHRAFPVCDNKG 482 RGLH++REY VDP ERM VEE+MT P T++ +L E HR +PV D Sbjct: 422 RGLHVHREYGVDPLERMHVEELMTAAPQTLAGALPAAEALAWCRAQEHVHRYYPVTDGV- 480 Query: 483 KLIGMLDRHALLGHLDKHAAATPVGELLTSSTSLLAFSTETCQSIARRMAAHHVERLPVV 542 L+GML AL +HA A+ + L + +S A MA V RLPVV Sbjct: 481 HLVGMLALRAL-----EHADASQPCDTLAQGHEHYLLPHMSARSAAGMMARLGVARLPVV 535 Query: 543 ADASSLQLIGIVSRSDLVKPAHTVFHEEHVRERL 576 A +L+G++S SDL + + EE VRERL Sbjct: 536 AGPERRKLVGVLSLSDLARASAAHAEEESVRERL 569 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 112 bits (279), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 138/514 (26%), Positives = 234/514 (45%), Gaps = 38/514 (7%) Query: 68 VIVPVIGGLIVGLMARFGSDKIRGHGIPEAIEAILFGKSKMSGKVAVLKPLASGIVIGSG 127 V++P GG++ GL + G+G + +EA+ G + + +L+ ++S I SG Sbjct: 50 VLLPTCGGVVAGLFLVWAKRTPAGNG-GDYMEAVAIGDGAIPVRNTLLRSISSLASIASG 108 Query: 128 GPFGAEGPIIMTGGAIGSLIAQHFHVSAAERKTLLVAGATAGMTAVFGTPVAAVLLAVEL 187 G G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A E+ Sbjct: 109 GSIGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEI 168 Query: 188 LLFEWRPRSLLPVILACAVAGFARPLLLEPG-------PLFPLHTDPVGLSALLVCIVAG 240 +L S PV++A VA L PG P FP +G +++ +V G Sbjct: 169 VLGSLVMESFGPVVVASVVANITMREL--PGYRAAYEMPYFP----EIGGWEVMLFLVLG 222 Query: 241 LLSGALSSAMSMALYWIEDRFHALPLHWMWWPALGAVAVGIGGYFEPRALGVGYDVIADL 300 +L+G L+ L + F L L +G + VG+ P G GY ++ L Sbjct: 223 VLAGVLAPQFLRVLELGKHGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSL 282 Query: 301 LNHHLAMSAVLALLVVKAIIWVIALGSGTSGGVLAPLLMLGAGL----GTAIAYVVPG-- 354 L+ AVL +LVVK + I +GSG GG+ P L +GA + G A ++P Sbjct: 283 LHTSWVWQAVLMVLVVKVLATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHI 342 Query: 355 SDPALWPLVCMAAVLSGVLGAPLTAAVFALGLTGDFNAFLPLLLATGISYGFTVLVMRRS 414 S P + +V M A L+ APL A + +T + LPL+L+ ++Y V+ RS Sbjct: 343 SQPFAYAMVGMGAFLAAASYAPLMAILMIFEMTLSYQVVLPLMLSCVVAY-----VVARS 397 Query: 415 IMTEKIARRGLHIYREYSVDPQERM-----FVEEVMTREPVTISASLSLEEIATVYFGAT 469 + + L +R D +ER+ + E++ + SLEEI+ ++ Sbjct: 398 VDGRSMYEITLKRHR----DNEERLRLRGTHMSELIRPAETVLHEEASLEEISALFLKYP 453 Query: 470 QQHRAFPVCDNKGKLIGMLDRHALLGH-LDKHAAATPVGELLTSSTSLLAFSTETCQSIA 528 ++ + D + + +G++ + LDK AA V L + + A Sbjct: 454 VKY--VYIVDGRQRYLGVVALQDITSWLLDKKEAAGRVARDFLRPHFLHEVTPDMSLGEA 511 Query: 529 RRM-AAHHVERLPVVADASSLQLIGIVSRSDLVK 561 ++ H ERLPV+A A QL+G+V ++ L+ Sbjct: 512 LQLFLDHQGERLPVIASADDPQLLGVVFKTSLLD 545 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 56.2 bits (134), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 74/281 (26%), Positives = 117/281 (41%), Gaps = 39/281 (13%) Query: 127 GGPFGAEGPIIMTGGAIGSLIAQHFHVSAAERKTLLVAGATAGMTAVFGTPVAAVLLAVE 186 G G EG + GGA+ + + +R+ LL+AG +AG ++VFGTP+A + +E Sbjct: 113 GASVGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLE 172 Query: 187 LL-LFEWRPRSLLPVILACAVA----------------GFARPLLLEPGPLFPLHTDPVG 229 +L + R ++ P +A A + P+ L G Sbjct: 173 VLAIGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFG 232 Query: 230 LSALLVCIVAGLLSGALSSAMSMALYWIEDRFHALPLHWMWWPALGA--VAVGIGGYFEP 287 L+ ++ LS + +S A PL P +G VA+ + Sbjct: 233 LAGMIFANSTHTLSAFMKRHVSYA-----------PLR----PFIGGFIVALAVWAVGTH 277 Query: 288 RALGVGYDVIADLLNHHLAMSAVLALLVVKAIIWVIALGSGTSGGVLAPLLMLGAGLGTA 347 R +G+G VI + LA L K + V +LGSG GG + PL +GA LG A Sbjct: 278 RYIGLGIPVIVEAFQQPLAPWDFLG----KMVFTVTSLGSGFKGGEVTPLFYIGATLGNA 333 Query: 348 IAYVVPGSDPALWPLVCMAAVLSGVLGAPLTAAVFALGLTG 388 +A ++ P L + AV +G PL + + A+ L G Sbjct: 334 LAPLLHLPFPLL-AGIGFVAVFAGAANTPLASTIMAIELFG 373 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,542,553 Number of extensions: 58524 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 374 Number of HSP's successfully gapped: 4 Length of query: 579 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 489 Effective length of database: 1,193,720 Effective search space: 583729080 Effective search space used: 583729080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)