BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0214.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 786 0.0 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 214 2e-56 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 207 2e-54 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 187 1e-48 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 167 2e-42 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 786 bits (2030), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/436 (89%), Positives = 413/436 (94%) Query: 4 PLSASDLSAYWMPYTANRNFKANPRMLVSAEGMYYRDDAGNAILDGTAGLWCVPLGHAQP 63 P S LSAYWMP+TANRNFKA PRMLVSAEGM+YRDDAGN ILDGTAGLWCVPLGHA P Sbjct: 6 PTHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHP 65 Query: 64 KIVSAVQKAVATLDYAPSFQLGHPMAFELAERLKQYTGGRYSQVFYTGSGSEAVDTALKI 123 KIVSAVQK+VATLDYAPSFQLGHP AFELAERLK YTG R+ VFYTGSGSEAVDTALKI Sbjct: 66 KIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKI 125 Query: 124 ALAYHRSRGDATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFNSALLQGVDHLPHTHNL 183 ALAYHR++G+ATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTF++ALL GVDHLPHTHNL Sbjct: 126 ALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNL 185 Query: 184 EKNAFTKGEPEYGTHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLQRLRDI 243 EKNAFTKGEPEYG HLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYL+RLR+I Sbjct: 186 EKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREI 245 Query: 244 CTKHGILLIFDEVITGFGRLGTPFASDYFGVEPDIFTTAKGLTNGVVPMGAVFVKPHIHD 303 CTKHGILLIFDEVITGFGRLGTPFASDYF VEPDIFTTAKGLTNGVVPMGAVFVKP IHD Sbjct: 246 CTKHGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHD 305 Query: 304 AFMNGPDGIELFHGYTYSGHPLACAAGLASLDIFEQDGILEHANGVADYFQEAAHALRGL 363 AFMNGPDGIELFHGYTYSGHPLACAAGLASL++F+ +G+LE+A VADYFQEAAHALRGL Sbjct: 306 AFMNGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYFQEAAHALRGL 365 Query: 364 PHVIDIRTIGMIVGIELSSIPGKVGARAYDAFRRAFNDGILIRVTGDIIALSPPLIIEKK 423 PHVID+RTIG+I GIEL+SIPG+VGARAYDAF RAFNDGILIRVTGDIIALSPPLII K+ Sbjct: 366 PHVIDVRTIGLIAGIELASIPGRVGARAYDAFVRAFNDGILIRVTGDIIALSPPLIINKQ 425 Query: 424 HIDELFGKLAAILKTL 439 IDELFGKLAAILKTL Sbjct: 426 QIDELFGKLAAILKTL 441 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 214 bits (544), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 146/435 (33%), Positives = 218/435 (50%), Gaps = 19/435 (4%) Query: 15 MPYTANRNFKANPRMLVSAEGMYYRDDAGNAILDGTAGLWCVPLGHAQPKIVSAVQKAVA 74 M + +RN ++ M V+ EGM D G +D + G LGH P+++ A++K V Sbjct: 1 MTHVFHRNPRSKLPMAVAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVG 60 Query: 75 TLDYAPSFQLGHPMAFELAERLKQYTGGRYSQVFYTGSGSEAVDTALKIALAYHRSRGDA 134 L YA + A ELA L G + V++ GSEAV+ ALK+A Y+ G Sbjct: 61 ELAYAHTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQP 120 Query: 135 TRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFNSALLQGVDHLPHTHNLEKNAFTKGEPE 194 R +I R + YHG G +++GG R+ F L++ H+ + A + + + Sbjct: 121 QRRHIIARRQSYHGNTLGALAIGGNAWRREMFMPMLIEA-HHVSPCYAYRNRADGESDVQ 179 Query: 195 YGTHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKG-YLQRLRDICTKHGILLIF 253 Y LADELE+ + A + A + E V G+T +PP G Y +++R +C K+G+LLI Sbjct: 180 YVQRLADELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLIL 239 Query: 254 DEVITGFGRLGTPFASDYFGVEPDIFTTAKGLTNGVVPMGAVFVKPHIHDAFMNGPDGIE 313 DEV++G GR G FA + GV PDI AKGL G P+GA+ HI+DA + G + Sbjct: 240 DEVMSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQ 299 Query: 314 LFHGYTYSGHPLACAAGLASLDIFEQDGILEHANGVADYFQ-EAAHALRGLPHVIDIRTI 372 HG+TY GH ACAA +A +++ +LE+ + + E A HV DIR Sbjct: 300 --HGHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGR 357 Query: 373 GMIVGIEL-----SSIPGKVGARAYDAFR-RAFNDGILIR--------VTGDIIALSPPL 418 G+ VG+EL S +P R + + A G+L+ GD I L+PP Sbjct: 358 GLFVGVELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPF 417 Query: 419 IIEKKHIDELFGKLA 433 I I E+ +L+ Sbjct: 418 IASSNDISEIVQRLS 432 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 207 bits (526), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 143/446 (32%), Positives = 213/446 (47%), Gaps = 21/446 (4%) Query: 5 LSASDLSAYWMPYTANRNFKA-NPRMLVSAEGMYYRDDAGNAILDGTAGLWCVPLGHAQP 63 L+A D P T+ R + P +L S +G Y D +LD +GLWCV G+ Q Sbjct: 6 LAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQE 65 Query: 64 KIVSAVQKAVATLDYAPS-FQLGHPMAFELAERLKQYTGGRYSQVFYTGSGSEAVDTALK 122 ++ A + + L YA F A LA++L + V++T GS+AVD A++ Sbjct: 66 SVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIR 125 Query: 123 IALAYHRSRGDATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFNSALLQGVDHLPHTHN 182 YH S G + I +RGYHG G + + FN L P H Sbjct: 126 YITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPL-------PTQHY 178 Query: 183 LEK----NAFTKGEPEYGTHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLQ 238 L + +G+ L V A N+AA EP+ GS GV++PPKG+L+ Sbjct: 179 LPSPYPYRSELQGDEAIIAASVQALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLK 238 Query: 239 RLRDICTKHGILLIFDEVITGFGRLGTPFASDYFGVEPDIFTTAKGLTNGVVPMGAVFVK 298 ++ C + IL + DEVITGFGR G FA V+PD+ T AKGLT G PMGAV + Sbjct: 239 AMQLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMS 298 Query: 299 PHIHDAFMNG-PDGIELFHGYTYSGHPLACAAGLASLDIFEQDGILEHANGVADYFQEAA 357 +++ +G P + HG TYS HP++ A L L ++E+ G+L++ + F Sbjct: 299 DRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGL 358 Query: 358 HALRGLPHVIDIRTIGMIVGIELSSIPGKVGARAYD------AFRRAFNDGILIRVTGD- 410 AL P V D R+ G++ +EL + A + F+ A+ G++ R GD Sbjct: 359 RALLAHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDN 418 Query: 411 IIALSPPLIIEKKHIDELFGKLAAIL 436 I+ +P L ++ +F +L AIL Sbjct: 419 ILGFAPALCYTQEDFALMFARLKAIL 444 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 187 bits (476), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 133/447 (29%), Positives = 212/447 (47%), Gaps = 14/447 (3%) Query: 2 NKPLSASDLSAYWMPYTANRNFKANPRMLVS-AEGMYYRDDAGNAILDGTAGLWCVPLGH 60 + L A L + W P T R ++ P + ++ A G++ D G LD + W GH Sbjct: 6 SSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGH 65 Query: 61 AQPKIVSAVQKAVATLDYAPSFQLGHPMAFELAERLKQYTGGRYSQVFYTGSGSEAVDTA 120 +I +A+ + + L++A H L+E+L TG FY G+ AV+ A Sbjct: 66 TNTRINAALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIA 125 Query: 121 LKIALAYHRSRGDATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFNSALLQGVDHLPHT 180 LK+++ R+ G + I GYHG G + V R + S L H+ + Sbjct: 126 LKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPA--HVAAS 183 Query: 181 HNLEKNAFTKGEPEYGTHLADELERLVTLHDASNIAAVIVEP-VAGSTGVLIPPKGYLQR 239 + + A + E D++ER++ IAA+IVEP V + G+ + GY++ Sbjct: 184 PDARQAAPGESAEEVAARALDDVERILQ-QRQGRIAALIVEPLVQCAAGMAMHDPGYVRG 242 Query: 240 LRDICTKHGILLIFDEVITGFGRLGTPFASDYFGVEPDIFTTAKGLTNGVVPMGAVFVKP 299 LR +C ++G LI DE+ G GR G+ FA + GV PD+ +KG++ G +P+ V Sbjct: 243 LRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSE 302 Query: 300 HIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASLDIFEQDGILEHANGVADYFQEAAHA 359 I+ AF + H ++Y+G+PLAC A LA+L IF +D +LE A A Sbjct: 303 AIYQAFYDDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQP 362 Query: 360 LRGLPHVIDIRTIGMIVGIELSSIPGKVGARAYDAFRR-----AFNDGILIRVTGDIIAL 414 L P V +R GMI + + + G A A AF R A +L+R G + L Sbjct: 363 LAQDPRVRHVRQRGMIWAYD-AVLEGS--AAATTAFSRRMAAEAAQREVLVRPIGASLYL 419 Query: 415 SPPLIIEKKHIDELFGKL-AAILKTLD 440 PP +++++ L +L AA T++ Sbjct: 420 MPPYLLDEEQAHWLGRQLHAAFAATME 446 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 167 bits (422), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 129/409 (31%), Positives = 201/409 (49%), Gaps = 35/409 (8%) Query: 40 DDAGNAILDGTAGLWCVPLGHAQPKIVSAVQKAVATLDYAPSFQLGHPMAFELAERLKQY 99 D G +D AG+ + GH PK++ A++ + + + + ELAER+ + Sbjct: 40 DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAERINRL 99 Query: 100 TGGRY-SQVFYTGSGSEAVDTALKIALAYHRSRGDATRTRLIGRERGYHGVGFGGMSVGG 158 T G Y + + +G+EAV+ A+KIA R R +I G+HG GM++ G Sbjct: 100 TPGNYPKKTAFFSTGAEAVENAIKIA------RAHTGRPGVIAFAGGFHGRTMMGMALTG 153 Query: 159 IGGNRKTFNSALLQGVDHLPHTHNLEKNAFTKGEPEYGTHLADELERLVTLH----DASN 214 K V H P+ L +G D LE + L +A Sbjct: 154 KVAPYKLGFGPFPGDVFHAPYPSAL-----------HGITSEDALEAVKGLFKSDIEAKR 202 Query: 215 IAAVIVEPVAGSTGVLIPPKGYLQRLRDICTKHGILLIFDEVITGFGRLGTPFASDYFGV 274 +AA+I+EPV G G P +++ LR +C +HGILLI DEV +G+GR G FA +++ V Sbjct: 203 VAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYDV 262 Query: 275 EPDIFTTAKGLTNGVVPMGAVFVKPHIHDAFMNGPDGIELFHGYTYSGHPLACAAGLASL 334 PD+ T AK L G +P+ AV + I DA P G+ G TY+G+PLA A+ LA L Sbjct: 263 LPDLMTMAKSLAGG-MPLSAVNGRAEIMDA--PAPGGL----GGTYAGNPLAIASALAVL 315 Query: 335 DIFEQDGILEHANGVADYFQEAAHALR-GLPHVIDIRTIGMIVGIELSS-IPGKVGARAY 392 D+ E++ ++ + D QE LR +P + ++R +G +V +E + GK A Sbjct: 316 DVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEYT 375 Query: 393 DAFRR-AFNDGILIRVT---GDIIALSPPLIIEKKHIDELFGKLAAILK 437 ++ A N+G+L+ G++I PL I +DE G LA ++ Sbjct: 376 KKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILAKAIR 424 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,438,914 Number of extensions: 63965 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 11 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)