BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0213.p (271 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 432 e-122 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 323 9e-90 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 263 1e-71 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 124 7e-30 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 116 2e-27 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 432 bits (1112), Expect = e-122, Method: Compositional matrix adjust. Identities = 215/265 (81%), Positives = 235/265 (88%), Gaps = 17/265 (6%) Query: 1 VAVARVALGTAEEVNDAVATAVAAFPAWSNTPPLXVAAFPAWSNTP---RARVLFKFLNL 57 V VA+VALGT++EV+ AV VAAFPAW SNTP RARVLF+FL+L Sbjct: 30 VPVAQVALGTSDEVDAAVKAGVAAFPAW--------------SNTPPLTRARVLFRFLHL 75 Query: 58 IQQHADDFAAILTREHGKTFSDAQGEVARGIEVVEYAVGIPQMLKGEYTDQIARGIDAWS 117 IQQ ADDFA I+ REHGKTFSDAQGEVARGIE+VE+A GIPQMLKGEYTDQIARGIDAWS Sbjct: 76 IQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQIARGIDAWS 135 Query: 118 MRQPLGVVAGITPFNFPAMVPMWMFPIALACGNCFILKPSERDPSASLLIAELLQQAGLP 177 MRQ LGVVAGITPFNFPAMVPMWMFPIA+ACGNCF+LKPSERDPSASLLIA+LL++AGLP Sbjct: 136 MRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIADLLKEAGLP 195 Query: 178 DGVFSVIQGDKVTVDALLDHPKVKAISFVGSTPIAEYIYARGSAKGKRVQALGGAKNHMV 237 DGVF+V+QGDKVTVDALLDHP+VKAISFVGSTPIAEYIYARGSAKGKRVQALGGAKNHMV Sbjct: 196 DGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQALGGAKNHMV 255 Query: 238 VMPDADMQVAVDALIGAAYXCSGRR 262 VMPDADMQ+AVDAL+GAAY +G R Sbjct: 256 VMPDADMQLAVDALMGAAYGSAGER 280 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 323 bits (828), Expect = 9e-90, Method: Compositional matrix adjust. Identities = 169/265 (63%), Positives = 205/265 (77%), Gaps = 15/265 (5%) Query: 2 AVARVALGTAEEVNDAVATAVAAFPAWSNTPPLXVAAFPAWSNTPRARVLFKFLNLIQQH 61 A A+VAL + +EVN AVA A AA PAW+ T PL RARVLFKF LI+Q+ Sbjct: 33 ATAKVALASVQEVNAAVAAASAAAPAWAETAPLK-----------RARVLFKFKELIEQN 81 Query: 62 ADDFAAILTREHGKTFSDAQGEVARGIEVVEYAVGIPQMLKGEYTDQIARGIDAWSMRQP 121 D A +T EHGK FSDA+GEV RG+E+VE+A GIPQ+LK ++TD I GID W++RQP Sbjct: 82 HDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDNIGGGIDNWNLRQP 141 Query: 122 LGVVAGITPFNFPAMVPMWMFPIALACGNCFILKPSERDPSASLLIAELLQQAGLPDGVF 181 LGV AGITPFNFP MVP+WM PIA+A GN F+LKPSERDPS SL++AEL ++AGLPDGVF Sbjct: 142 LGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSLMLAELFKRAGLPDGVF 201 Query: 182 SVIQGDKVTVDALLDHPKVKAISFVGSTPIAEYIYARGSAKGK----RVQALGGAKNHMV 237 +V+QGDKV VDALL+HP+V+A+SFVGSTPIAEYIY + + + R QALGGAKNH+V Sbjct: 202 NVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATERAGTFPLRAQALGGAKNHLV 261 Query: 238 VMPDADMQVAVDALIGAAYXCSGRR 262 VMPDA++ AVDALIGAAY +G R Sbjct: 262 VMPDANLDQAVDALIGAAYGSAGER 286 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 263 bits (671), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 127/260 (48%), Positives = 172/260 (66%), Gaps = 11/260 (4%) Query: 3 VARVALGTAEEVNDAVATAVAAFPAWSNTPPLXVAAFPAWSNTPRARVLFKFLNLIQQHA 62 VARV T EEV+ AVA A F W NT S R R++ KF L++++ Sbjct: 37 VARVPFATKEEVDRAVANAKETFKTWRNT-----------SLAQRMRIMLKFQQLLRENI 85 Query: 63 DDFAAILTREHGKTFSDAQGEVARGIEVVEYAVGIPQMLKGEYTDQIARGIDAWSMRQPL 122 A ++TREHGKT DA+GEV RG+EVVE+A I + GE + +A G+D +++ QPL Sbjct: 86 APLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAENVAGGVDVYTLYQPL 145 Query: 123 GVVAGITPFNFPAMVPMWMFPIALACGNCFILKPSERDPSASLLIAELLQQAGLPDGVFS 182 GV AGIT FNFP M+P +MFPIA+ACGN F+LKPSE+DP++SL + EL QAGLP GV + Sbjct: 146 GVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLVELANQAGLPPGVLN 205 Query: 183 VIQGDKVTVDALLDHPKVKAISFVGSTPIAEYIYARGSAKGKRVQALGGAKNHMVVMPDA 242 V+ G + + DHP +KA+SF+GST + +IY R S GKR Q + GAKNH +V+PDA Sbjct: 206 VVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQCMMGAKNHCIVLPDA 265 Query: 243 DMQVAVDALIGAAYXCSGRR 262 A++ L+GAA+ +G+R Sbjct: 266 PKDQAINNLLGAAFGAAGQR 285 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 124 bits (312), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 77/230 (33%), Positives = 118/230 (51%), Gaps = 21/230 (9%) Query: 39 FPAWS---NTPRARVLFKFLNLIQQHADDFAAILTREHGKTFSDAQGE----VARGIEVV 91 P W RAR L+ +Q+HA A + T ++GK +++ VAR Sbjct: 81 LPGWQALGGHGRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVAR--HFY 138 Query: 92 EYAVGIPQMLKGEYTDQIARGIDAWSMRQPLGVVAGITPFNFPAMVPMWMFPIALACGNC 151 YA G Q+ + E+ D + P+GVV I P+NFP ++ W ALA GN Sbjct: 139 HYA-GWAQLQESEFPDHV-----------PVGVVGQIVPWNFPLLMLAWKIAPALALGNT 186 Query: 152 FILKPSERDPSASLLIAELLQQAGLPDGVFSVIQGDKVTVDALLDHPKVKAISFVGSTPI 211 +LKP+E +LL AEL QQAGLP GV +++ GD T A++ HP ++ I+F GST + Sbjct: 187 VVLKPAENTSLTALLFAELAQQAGLPAGVLNIVTGDGATGAAVVAHPGIQKIAFTGSTEV 246 Query: 212 AEYIYARGSAKGKRVQALGGAKNHMVVMPDADMQVAVDALIGAAYXCSGR 261 I + + GK + G K+ +V DAD+ A++ ++ A + G+ Sbjct: 247 GRLIREQTAGSGKSLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQ 296 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 77/260 (29%), Positives = 130/260 (50%), Gaps = 12/260 (4%) Query: 3 VARVALGTAEEVNDAVATAVAAFPAWSNTPPLXVAAFPAWSNTPRARVLFKFLNLIQQHA 62 + V L A E A+ A AA+PAW + R+ +L ++ L+ + Sbjct: 40 LGHVPLMGAAETRRAIEAANAAWPAWKKK-----------TAKERSAILRRWYELMLANT 88 Query: 63 DDFAAILTREHGKTFSDAQGEVARGIEVVEYAVGIPQMLKGEYTDQIARGIDAWSMRQPL 122 DD A I+T E GK ++A+GE+ +E+ + G+ + +++ + Sbjct: 89 DDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAI 148 Query: 123 GVVAGITPFNFPAMVPMWMFPIALACGNCFILKPSERDPSASLLIAELLQQAGLPDGVFS 182 GV A ITP+NFPA + ALA G +LKP+E P ++L +A L ++AG+P GVFS Sbjct: 149 GVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVFS 208 Query: 183 VIQGDKVTVDA-LLDHPKVKAISFVGSTPIAEYIYARGSAKGKRVQALGGAKNHMVVMPD 241 V+ G + + +P V+ ISF GST + + + + ++ K++ G +V D Sbjct: 209 VVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDD 268 Query: 242 ADMQVAVDALIGAAYXCSGR 261 AD+ AV+ I + Y +G+ Sbjct: 269 ADLDAAVEGAIASKYRNAGQ 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 832,448 Number of extensions: 35080 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 19 Length of query: 271 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 187 Effective length of database: 1,222,130 Effective search space: 228538310 Effective search space used: 228538310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)