BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0212.p (201 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 374 e-105 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 298 3e-82 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 182 1e-47 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 92 2e-20 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 90 1e-19 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 374 bits (959), Expect = e-105, Method: Compositional matrix adjust. Identities = 179/201 (89%), Positives = 190/201 (94%) Query: 1 LVSALAERTRNLKINDGMEKDAEMGPVVTAAAKARIEGLIGKGVEEGATLVVDGRGFKVP 60 LV+ALAER R LKIN+GM AEMGPVVTAAAK RIEGLIG+GVEEGATLVVDGRG++VP Sbjct: 297 LVAALAERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVP 356 Query: 61 GRENGFFVGGTLFDHVTPEMTIYKEEIFGPVLCVVRAPDVATAVELINNHEYGNGVAVFT 120 G E GFFVGGTL DHVTPEMT+YKEEIFGPVLCV+R PD+A+AVELIN HEYGNGVAV+T Sbjct: 357 GFEKGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYT 416 Query: 121 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHVYGPEGVRFYTRAKA 180 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHH+YGPEGVRFYTRAKA Sbjct: 417 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKA 476 Query: 181 VMQRWPNTASAGAEFAFPQMK 201 VMQRWPNTASAGAEFAFPQMK Sbjct: 477 VMQRWPNTASAGAEFAFPQMK 497 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 298 bits (762), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 139/201 (69%), Positives = 161/201 (80%) Query: 1 LVSALAERTRNLKINDGMEKDAEMGPVVTAAAKARIEGLIGKGVEEGATLVVDGRGFKVP 60 LV AL R + LK+ +GME DAEMGP+VTAA KA++EG I GV+ GA L+VDGRG KVP Sbjct: 303 LVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVP 362 Query: 61 GRENGFFVGGTLFDHVTPEMTIYKEEIFGPVLCVVRAPDVATAVELINNHEYGNGVAVFT 120 G E GFF+GGTLFDHVT EM IY+EEIFGPVLCVVR PD A+AV+LIN HEYGNGV++FT Sbjct: 363 GHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFT 422 Query: 121 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHVYGPEGVRFYTRAKA 180 DG A EF R+I+VGMVG+NVP+PVPMA+ SFGGWKRSLFGD H YG EG+RFYTR K+ Sbjct: 423 ADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKS 482 Query: 181 VMQRWPNTASAGAEFAFPQMK 201 +MQRW T GAEF P K Sbjct: 483 IMQRWSATIGKGAEFTMPVAK 503 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 182 bits (463), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 89/202 (44%), Positives = 130/202 (64%), Gaps = 2/202 (0%) Query: 2 VSALAERTRNLKINDGMEKDAEMGPVVTAAAKARIEGLIGKGVEEGATLVVDGRGFKVPG 61 + + ER++ +K+ G ++ A++GP+V+ AAK R+E LI GVE+GA L++DGR KV G Sbjct: 302 IPEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAG 361 Query: 62 RENGFFVGGTLFDHVTPEMTIYKEEIFGPVLCVVRAPDVATAVELINNHEYGNGVAVFTR 121 ENG FVG T+F V PEM IY +EIFGP +C+V + A+ IN + GNG ++FT Sbjct: 362 SENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTS 421 Query: 122 DGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHVYGPEGVRFYTRAKAV 181 G AR+F +I+VG VG+NVP+PVP+A+ SF G + S GD G + V F+T+ K V Sbjct: 422 SGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTV 481 Query: 182 MQRW--PNTASAGAEFAFPQMK 201 RW P+ + G P+++ Sbjct: 482 TARWYAPDEEAGGQHHHHPEVR 503 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 92.4 bits (228), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 64/181 (35%), Positives = 86/181 (47%), Gaps = 9/181 (4%) Query: 1 LVSALAERTRNLKINDGMEKDAEMGPVVTAAAKARIEGLIGKGVEEGATLVVDGRGFKVP 60 V LA + +L + D + +G VV A R LI + +GATLV GR Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDT- 359 Query: 61 GRENGFFVGGTLFDHVTPEMTIYKEEIFGPVLCVVRAPDVATAVELINNHEYGNGVAVFT 120 + TL DHV+ M IY+EE FGPV +VR A+ N++EYG A+F+ Sbjct: 360 ------LMPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFS 413 Query: 121 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHVYGPEGVRFYTRAKA 180 RD A R+IE G+ +N P A FGG K S FG H G G+ +T + Sbjct: 414 RDVARALALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG--HFGGKAGIAEFTELRW 471 Query: 181 V 181 V Sbjct: 472 V 472 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 58/172 (33%), Positives = 89/172 (51%), Gaps = 5/172 (2%) Query: 11 NLKINDGMEKDAEMGPVVTAAAKARIEGLIGKGVEEGATLVVDGRGFKVPGRENGFFVGG 70 +K+ D + GP+ A R++ +I G+++GA L+ G G + G G++ Sbjct: 316 QMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPG-RPDGLAQGYYARP 374 Query: 71 TLFDHVTPEMTIYKEEIFGPVLCVVRAPDVATAVELINNHEYGNGVAVFTRDGGVAREFV 130 T+F V +M I +EEIFGPVL ++ V A+ + N+ YG G V RD R Sbjct: 375 TIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVA 434 Query: 131 RQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHVYGPEGVRFYTRAKAVM 182 +I+ G V +N P P A FGG+K+S G+ YG EG+ Y KA++ Sbjct: 435 ARIQSGQVHLNYPAWDPQA--PFGGYKQS--GNGREYGVEGMEEYMEVKAIL 482 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 670,364 Number of extensions: 30082 Number of successful extensions: 116 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 16 Length of query: 201 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 120 Effective length of database: 1,236,335 Effective search space: 148360200 Effective search space used: 148360200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)