BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0209.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC t... 445 e-126 gi|300314069|ref|YP_003778161.1| amino acid ABC transporter perm... 153 2e-38 gi|300312953|ref|YP_003777045.1| amino acid ABC transporter perm... 126 2e-30 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 124 1e-29 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 102 5e-23 >gi|300313933|ref|YP_003778025.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 334 Score = 445 bits (1144), Expect = e-126, Method: Compositional matrix adjust. Identities = 265/322 (82%), Positives = 290/322 (90%), Gaps = 5/322 (1%) Query: 1 MKHIAHFRFTLLAIAVVLLLPLCVGSGTLATEVLIYALAALGCNLLLGYTGLMSFGQGIF 60 MK IAHFR+TLLA AV LLLPL + SGTLATEVL++ALAALGCNLLLGYTGLMSFGQGIF Sbjct: 1 MKQIAHFRYTLLAAAVALLLPLLLPSGTLATEVLVFALAALGCNLLLGYTGLMSFGQGIF 60 Query: 61 FGIGSYATGLALLHLKAPLLISLAIAAVIGGLIALLVGWFSIRQRGTYFVMLTLAFAQMF 120 FG+GSY G+ LL LK LL +LA+AA+ G L+ALLVGWFSIRQRGTYFVMLTLAFAQMF Sbjct: 61 FGVGSYTAGVVLLQLKLALLPALALAALGGALVALLVGWFSIRQRGTYFVMLTLAFAQMF 120 Query: 121 YFLAYTMKDITGGDNGLLDIPRPNLSVLGHTLLPTTTSWQYYSFVAILFVVVFWLLQRVV 180 YFLAYTMKD+TGGDNGLLDIPRP L++ G TLLPTT+SWQYY+ VAILFV+VFWLLQRVV Sbjct: 121 YFLAYTMKDVTGGDNGLLDIPRPPLALFGLTLLPTTSSWQYYTVVAILFVLVFWLLQRVV 180 Query: 181 DSVLGRTLLAVRDNEAXLKARASAVGYDVTLLKLAAFVISGAVTGLAGGLHAMMTGVAPL 240 DSVLG+TLLAVR+NE ARASA+GYDV LLKLAAFVISGAVTGLAGGLHAMMTGVAPL Sbjct: 181 DSVLGKTLLAVRENE----ARASALGYDVRLLKLAAFVISGAVTGLAGGLHAMMTGVAPL 236 Query: 241 SNIEYHTSESILIMTVIGGTGNLFASVLGASFYVLVGNWLSTLWPRWLMLLGFLLIGVSL 300 SNIEYHTSE+IL+MTVIGGTGNLFASVLGASFYVLVGNWLSTLWPRWLMLLG LLI VSL Sbjct: 237 SNIEYHTSEAILVMTVIGGTGNLFASVLGASFYVLVGNWLSTLWPRWLMLLGLLLIAVSL 296 Query: 301 YMHKGLYGLAQSLLRRLRPGEK 322 YM KGL+GLA ++ +R G K Sbjct: 297 YMQKGLFGLAMKIIDAVR-GNK 317 >gi|300314069|ref|YP_003778161.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 326 Score = 153 bits (387), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 107/301 (35%), Positives = 163/301 (54%), Gaps = 25/301 (8%) Query: 32 EVLIYALAALGCNLLLGYTGLMSFGQGIFFGIGSYATGLALLHLKAPLLISLAIAAVIGG 91 ++L +AL A NLL+GYTGL+SFG F G Y G AL P + L I + G Sbjct: 28 KMLCFALFACAFNLLIGYTGLLSFGHAAFLGWAGYMCGQALKVWGLPTELGLIIGTLTGA 87 Query: 92 LIALLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDITGGDNGLLDIPRPNLSVLGHT 151 LI L++G +IR++G YF M+TLA AQM +F+A TGG++GL +PR L LG Sbjct: 88 LIGLVMGLLAIRRQGIYFTMITLALAQMLFFVAL-QAPFTGGEDGLQGVPRGKL--LGFL 144 Query: 152 LLPTTTSWQYYSFVAILFVVVFWLLQRVVDSVLGRTLLAVRDNEAXLKARASAVGYDVTL 211 L + + YY +AI V F L+ R + S G+ L A+++NE RA ++GYDV Sbjct: 145 NLESDMTL-YYVVLAIA-VAGFALIVRTIHSPFGQVLKAIKENE----PRAISLGYDVAR 198 Query: 212 LKLAAFVISGAVTGLAGGLHAMMTGVAPLSNIEYHTSESILIMTVIGGTGNLFASVLGAS 271 KL AFV+S ++GLAG ++ G L+++ + S +++MT++GG G + ++GA Sbjct: 199 YKLLAFVLSAGLSGLAGATKTLVLGFETLTDVHWSMSGLVVLMTLVGGLGTILGPIVGAV 258 Query: 272 FYVLVGN----------------WLSTLWPRWLMLLGFLLIGVSLYMHKGLYGLAQSLLR 315 +++ N W +TL ++ GF+ I L KG+ G +L R Sbjct: 259 IIIMLENKLGDIGAWLASVTHIDWFNTLGDSVTIVTGFIFIVCVLAFRKGIVGEILALTR 318 Query: 316 R 316 + Sbjct: 319 K 319 >gi|300312953|ref|YP_003777045.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 317 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 104/295 (35%), Positives = 165/295 (55%), Gaps = 10/295 (3%) Query: 31 TEVLIYALAALGCNLLLGYTGLMSFGQGIFFGIGSYATGLALLHLKAPLLISLAIAAVIG 90 T +LI+A+ A N +LGY G++SFG +FFG+G+Y T +A H + +AA++ Sbjct: 33 TRILIFAMVAASLNFILGYGGMVSFGHAVFFGLGAYVTAIASFHGVTSAWLIWLLAALVT 92 Query: 91 GLIALLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDITGGDNGLLDIPRPNLSVLGH 150 L+ L++G ++R R YF+M+T+A AQ+FY+ + GGD+GL RP +LG Sbjct: 93 ALVGLVIGVIALRTRAVYFIMITMALAQLFYYFFVGFR-YYGGDDGLQVTARP---LLGF 148 Query: 151 TLLPTTTSWQYYSFVAILFVVVFWLLQRVVDSVLGRTLLAVRDNEAXLKARASAVGYDVT 210 L + + Y+ +A L F LLQR++ S G L A+R NE RA A+GY Sbjct: 149 GLDMSGDNAFYWVVLAWL-AASFILLQRLLASRFGLALNAIRQNE----RRAQAIGYPAL 203 Query: 211 LLKLAAFVISGAVTGLAGGLHAMMTGVAPLSNIEYHTSESILIMTVIGGTGNLFASVLGA 270 KLAAFVI+ + G+AG L M A + + S +L+M +GG G + +LGA Sbjct: 204 RYKLAAFVIAAVIAGIAGSLIGMSNLFASPKLLHWSQSGILLVMVALGGVGYFYGGLLGA 263 Query: 271 SFYVLVGNWLSTLWPRWLMLLGFLLIGVSLYMHKGLYGLAQSLLRRLRPGEKPSA 325 + ++L+ LST + W + +G +L+ V + +G+ L S+ + L+ GE+ A Sbjct: 264 AAFLLLEEVLSTHFEYWQIYVGLILLLVVMVAPRGVSSLV-SIGKTLKQGEQHGA 317 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 89/258 (34%), Positives = 127/258 (49%), Gaps = 17/258 (6%) Query: 29 LATEVLIYALAALGCNLLLGYTGLMSFGQGIFFGIGSYATGLALLHLKAPLLISLAIAAV 88 L E+L+ L A + L+G G++SFG +FG+G+YA L L L+ P+ +++ A Sbjct: 350 LMVEILVAVLFAASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAG 409 Query: 89 IGGLIALLVGWFSIRQRGTYFVMLTLAFAQMFYFLAYTMKDITGGDNGLLDIPRPNLSVL 148 L ALL GWF +R G Y MLTLAFAQ+ + Y D+TGG NGL+ + Sbjct: 410 TAMLAALLFGWFCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGL-------- 461 Query: 149 GHTLLPTTTSWQYYSFVAILFVVVFWLLQRVVDSVLGRTLLAVRDNEAXLKARASAVGYD 208 T + YY + + +LL+RV+ + G L A RD RA A+G D Sbjct: 462 -RTAAALASPLAYYYLALLCSALGVYLLRRVIHAPFGLALRAARDA----SGRAQALGMD 516 Query: 209 VTLLKLAAFVISGAVTGLAGGLHAMMTGVAPLSNIEYHTSESILIMTVIGGTGNLFASVL 268 V L+ F ++G GLAG L A G + S L+M ++GG +L +L Sbjct: 517 VRALQWGGFALAGLFCGLAGVLFAFSKGSISPEVAGVNRSVDGLVMVLLGGVQSLAGPLL 576 Query: 269 GASFYVLVGNWLSTLWPR 286 GAS + WL L R Sbjct: 577 GASVF----TWLQDLVAR 590 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 102 bits (254), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 83/281 (29%), Positives = 144/281 (51%), Gaps = 22/281 (7%) Query: 10 TLLAIAVVLLLPLCVGSG---TLATEVLIYALAALGCNLLLGYTGLMSFGQGIFFGIGSY 66 +LL I + +P+ V + L +LIYA+ G ++++GYTG +S G FGIGSY Sbjct: 9 SLLGIVALAGIPMVVHNPYYLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGSY 68 Query: 67 ATGLALLHLKAPLLIS----LAIAAVIGGLIALLVGWFSIRQRGTYFVMLTLAFAQMFYF 122 A G+ L P+ ++ +A+ AV G ++AL ++R G Y M+TLAF + Sbjct: 69 AAGVLFFKLGMPIFVTVPAAIAVTAVFGAILALP----ALRVTGPYLAMVTLAFGTIIQI 124 Query: 123 LAYTMKDITGGDNGLLDIPRPNLSVLGHTLLPTTTSWQYYSFVAILFVVVFWLLQRVVDS 182 L M +T G G + + +P + G + + Q++ VA+L V+ ++ R++ S Sbjct: 125 LINEMTFLTEGPLG-IKLQKPMIG--GDKM----SEVQFFYLVAVLMVLSMLVVHRILKS 177 Query: 183 VLGRTLLAVRDNEAXLKARASAVGYDVTLLKLAAFVISGAVTGLAGGLHAMMTGVAPLSN 242 LGR A+RD+ + +G V K+ AF+IS A+ GL+G L+A + Sbjct: 178 NLGRAFQALRDSPIA----SDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISPNT 233 Query: 243 IEYHTSESILIMTVIGGTGNLFASVLGASFYVLVGNWLSTL 283 + + L+ ++GG + S++GA V++ + LS + Sbjct: 234 YNFELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSDI 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 835,412 Number of extensions: 33260 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 22 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)