BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0196.p (110 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313911|ref|YP_003778003.1| cations and cationic drugs memb... 135 8e-34 >gi|300313911|ref|YP_003778003.1| cations and cationic drugs membrane transport protein [Herbaspirillum seropedicae SmR1] Length = 110 Score = 135 bits (340), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 64/110 (58%), Positives = 89/110 (80%) Query: 1 MHPYLLLGIAIVAEVIATSALRAAEGFTRLLPSIMVVIGYAVAFLCLSLTLKSIPVGIVY 60 M YL L IAIVAEVIAT+AL++++GFTRLLP ++VV GY V+F CL+LTL+++P + Y Sbjct: 1 MSAYLSLAIAIVAEVIATTALKSSQGFTRLLPGLIVVAGYGVSFYCLALTLRTMPTSVAY 60 Query: 61 AIWSGLGIVLISVVAYFLYGQSLDLPAIIGMALILVGVVVLNLFSKSTVH 110 AIWSG+G+VLI++ A+ ++ Q LD PA+ G+ALI+ GV+V+NLFS S H Sbjct: 61 AIWSGVGLVLITLAAWLIHHQRLDWPAVAGIALIVAGVMVINLFSSSAAH 110 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.145 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 328,353 Number of extensions: 12663 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 75 Number of HSP's successfully gapped: 1 Length of query: 110 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 37 Effective length of database: 1,274,215 Effective search space: 47145955 Effective search space used: 47145955 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 124 (52.4 bits)