BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0184.p (515 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Her... 434 e-122 gi|300313944|ref|YP_003778036.1| cell morphogenesis actin-like A... 403 e-113 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 74 3e-14 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 66 8e-12 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 63 5e-11 >gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 434 bits (1115), Expect = e-122, Method: Compositional matrix adjust. Identities = 210/293 (71%), Positives = 241/293 (82%), Gaps = 2/293 (0%) Query: 1 LFEELATQNGRRIGVATLASEKTLNALSLDMVHLLTPQLRQWARDPDIAMVLLQAQGEKA 60 +FEEL NGRRIG+ATLASEKTL+A+SL+MV LLTPQL+ W DP++AMV+LQAQGEKA Sbjct: 9 VFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVVLQAQGEKA 68 Query: 61 FCAGGDLQKIYTSMREHHASPARDDIRGNQYAAEFFEHEYRLDYEIHTYPKPILCWGHGI 120 FCAGGDLQ++Y SMREHHASPAR+DIR N+YAAEFFE EYRLDY IH++ KPILCWGHGI Sbjct: 69 FCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKPILCWGHGI 128 Query: 121 VMGGGIGLMAGCSHRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGKLGAFLALTGALM 180 VMGGGIGLMAGCSHRVVTE+SRLAMPEITIGLYPDVGGSWFL+R PGK+G FLALTGA + Sbjct: 129 VMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIFLALTGANL 188 Query: 181 NAEDAKFTGLADYRIAHADKQKVIDALQLQNWGDGNDAAVLGRVLQQAERDVAAE--VAF 238 NA+DA F GLADY +AHA K V DAL Q WG G DA +L RVL A+ E AF Sbjct: 189 NAQDALFAGLADYGMAHASKAAVFDALLSQPWGQGRDAELLDRVLAGAQAASRREGVAAF 248 Query: 239 APSALRENFDLINALCSGQTVPDIVDAILAHKTDNPWLQKAIATLTAGAPGAS 291 PS LR +FD I ALC T+P+IVDAILA +TD+ WL KA +TL AGAPG++ Sbjct: 249 VPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGSA 301 >gi|300313944|ref|YP_003778036.1| cell morphogenesis actin-like ATPase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 403 bits (1036), Expect = e-113, Method: Compositional matrix adjust. Identities = 217/238 (91%), Positives = 222/238 (93%) Query: 278 KAIATLTAGAPGASQVYLIEEPLGAGASQVYLIEEPMAAAIGSGLPVSDATGSMVVDIGG 337 + I + G+ + + E LGAGASQVYLIEEPMAAAIGSGLPVSDATGSMVVDIGG Sbjct: 110 RIIICVPCGSTQVERRAIRESALGAGASQVYLIEEPMAAAIGSGLPVSDATGSMVVDIGG 169 Query: 338 GTTEVGIISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKKAIGSAFPG 397 GTTEVGIISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKK IGSAFPG Sbjct: 170 GTTEVGIISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKKEIGSAFPG 229 Query: 398 SEVKEMEVKGRNLSEGIPRSFTISSNEILEALTDPLNNIVSAVKNALEQTPPELGADIAE 457 SEVKEMEVKGRNLSEGIPRSFTISSNEILEALTDPLNNIVSAVKNALEQTPPELGADIAE Sbjct: 230 SEVKEMEVKGRNLSEGIPRSFTISSNEILEALTDPLNNIVSAVKNALEQTPPELGADIAE 289 Query: 458 KGMMLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGMALDRMDKLGSIFSNE 515 KGMMLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGMALDRMDKLGSIFS E Sbjct: 290 KGMMLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGMALDRMDKLGSIFSAE 347 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 59/202 (29%), Positives = 91/202 (45%), Gaps = 22/202 (10%) Query: 2 FEELATQNGRRIGVATLASEKTLNALSLDMVHLLTPQLRQWARDPDIAMVLLQAQGEKAF 61 +E + + ++G+ L K LNALS ++ L L + +I +++ EKAF Sbjct: 3 YENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGS-EKAF 61 Query: 62 CAGGDLQKI--YTSMREHHASPARDDIRGNQYAAEFFEHEYRLDYEIHTYPKPILCWGHG 119 AG D+ + Y M + + EF + I KP++ G Sbjct: 62 AAGADISAMAGYDYM--------------DVFKGEFITRNWETLRRIR---KPVIAAVAG 104 Query: 120 IVMGGGIGLMAGCSHRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGKLGAF-LALTGA 178 +GGG L C + + ++ PEI +G+ P GG+ L R K A LALTG Sbjct: 105 YALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGR 164 Query: 179 LMNAEDAKFTGLADYRIAHADK 200 +M+AE+A+ GL RI ADK Sbjct: 165 MMDAEEAERAGLVS-RIVAADK 185 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 65.9 bits (159), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 54/203 (26%), Positives = 82/203 (40%), Gaps = 14/203 (6%) Query: 5 LATQNGRRIGVATLASEKTLNALSLDMVHLLTPQLRQWARDPDIAMVLLQAQGEKAFCAG 64 L ++ + I TL + NALS +M+ L QL D + V+L A G KAFCAG Sbjct: 9 LEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANG-KAFCAG 67 Query: 65 GDLQKIYTSMREHHASPARDDIRGNQYAAEFFEHEYRLDYEIHTYPKPILCWGHGIVMGG 124 DL R+ A+P + Y F L + P P++ G+ Sbjct: 68 HDL-------RQMQATPQQT------YYEALFHRCSHLMQSLRELPVPVIAKVQGLATAA 114 Query: 125 GIGLMAGCSHRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGKLGAFLALTGALMNAED 184 G L+A C + E +R A+ I +GL+ P K + +TG ++A Sbjct: 115 GCQLVATCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAAT 174 Query: 185 AKFTGLADYRIAHADKQKVIDAL 207 A GL + + + AL Sbjct: 175 AAAWGLINQAVPEQELDSATQAL 197 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 63.2 bits (152), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 53/187 (28%), Positives = 82/187 (43%), Gaps = 18/187 (9%) Query: 5 LATQNGRRIGVATLASEKTLNALSLDMVHLLTPQLRQWARDPDIAMVLLQAQGEKAFCAG 64 LA ++G + TL NAL + + L + D + +V++ EKAF AG Sbjct: 11 LAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISG-NEKAFAAG 69 Query: 65 GDLQKIYTSMREHHASPARDDIRGNQYAAEFFEHEYRLDYEIHTYPKPILCWGHGIVMGG 124 DL + M A + D+R + A I +PKPIL +G +G Sbjct: 70 ADLNE----MIHKDAIATQLDVRAQYWRA------------IARFPKPILAAVNGYALGA 113 Query: 125 GIGLMAGCSHRVVTERSRLAMPEITIGLYPDVGGSWFLSRTPGK-LGAFLALTGALMNAE 183 G L+ + +++ PEI +G P GG+ L RT GK L + L+G ++A+ Sbjct: 114 GCELLMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISAD 173 Query: 184 DAKFTGL 190 A GL Sbjct: 174 QALQAGL 180 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,622,923 Number of extensions: 71128 Number of successful extensions: 304 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 298 Number of HSP's successfully gapped: 7 Length of query: 515 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 426 Effective length of database: 1,198,455 Effective search space: 510541830 Effective search space used: 510541830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)