BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0177.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 840 0.0 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 180 3e-46 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 164 1e-41 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 144 2e-35 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 134 1e-32 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 840 bits (2171), Expect = 0.0, Method: Compositional matrix adjust. Identities = 423/487 (86%), Positives = 457/487 (93%) Query: 1 MHTKTIKELSTLLQTKKISAAELATLFLDRIGQSDLNAFLHVDRELTLQQAAQADLRIAN 60 MH T+KELS LQ +KISA ELATLFLDRI SDLNAFLHVDRELTL QA AD R+++ Sbjct: 1 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD 60 Query: 61 GDVTPLTGIPIAHKDLFVTRGWRTTAGSKMLENYVSPFDATVVEQFNAAGTVTLGKLNCD 120 + LTG+PIAHKD+FVTRGWRTTAGS+ML+NYVSPFDATVVEQFNAAG VTLGKLNCD Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD 120 Query: 121 EFAMGSGNENSYFGAVKNPWDKRAVPGGSSGGSAAAIAARLAPAATGTDTGGSIRQPSAL 180 EFAMGSGNENSYFG NPWDKRAVPGGSSGGSAAA+AARLAPAAT TDTGGSIRQP++L Sbjct: 121 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL 180 Query: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMSGFDQRDSTSIERPK 240 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAM+GFD+RDSTS++RPK Sbjct: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 Query: 241 EDFTRSLNDSIKGLRIGVPKEFFGAGLAADVEQAVRTALAEYEKLGATLVDISLPKTELS 300 EDFTRSLNDS++GLRIGVPKEFFGAGLAADVE AVR AL+E+EKLGA LV+ISLPKTELS Sbjct: 241 EDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPKTELS 300 Query: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYQKTRAEGFGDEVKRRILVGAYV 360 IPVYYVIAPAEASSNLSRFDGVRYGHRAADY +L DMY+K+RAEGFG+EVKRRILVG+YV Sbjct: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVGSYV 360 Query: 361 LSHGYYDAYYLQAQKIRRLIAQDFQNALTGPDRKCDVIMGPVSPTVAWDLGDKTDDPVAN 420 LSHGYYDAYYLQAQKIRRLIAQDFQNAL G +R+CDVIMGPVSPTVAWDLGDKT+DPVAN Sbjct: 361 LSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTNDPVAN 420 Query: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGQRPVGLQIIGSYYDEARLLNVAHQYQQVT 480 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNG+RPVGLQIIG+Y+DEARLLNVAHQ+QQ T Sbjct: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQAT 480 Query: 481 DWHLRAP 487 DWHLR P Sbjct: 481 DWHLREP 487 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 180 bits (456), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 137/430 (31%), Positives = 205/430 (47%), Gaps = 29/430 (6%) Query: 66 LTGIPIAHKDLFVTRGWRTTAGSKMLENYVSPFDATVVEQFNAAGTVTLGKLNCDEFAMG 125 L G + KD G GS LE +V +DAT+V + AG LGK C+ F + Sbjct: 88 LAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLDAGATILGKATCEHFCLS 147 Query: 126 SGNENSYFGAVKNPWDKRAVPGGSSGGSAAAIAARLAPAATGTDTGGSIRQPSALCGVTG 185 G+ S V NP GGSS GSAA +AA A A G D GGSIR PSA CG G Sbjct: 148 GGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAFCGTYG 207 Query: 186 IKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMSGFDQRDSTSIERPKEDFTR 245 +K T+G V G++ +++D GPI D L+L ++G D D + +T Sbjct: 208 MKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPRQYAPEVQPYTA 267 Query: 246 SLNDSIKGLRIGVPKEFFGAG-LAADVEQAVRTALAEYEKLGATLVDISLPKTELSIPVY 304 +L+ ++GL+IG+ E F G + V V+ A+A+ EKLGA++ +S+ + L+ ++ Sbjct: 268 ALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVSVEEHALAGALW 327 Query: 305 YVIAPAEASSNLSRFDGVRYGHRAADYKDLADM-------YQKTRAEGFGDEVKRRILVG 357 I + + +G+ + ++K L D+ + + A+ +K + VG Sbjct: 328 QPIGCEGLTMQMMHGNGMGF-----NWKGLYDLGLLQHHAHWREHADDLSATLKLCMFVG 382 Query: 358 AYVLSHGYYDAYYLQAQKIRRLIAQDFQNALTGPDRKCDVIMGPVSPTVAWDLGDKTDDP 417 Y L+ Y +Y +AQ I R + AL D+++ P P +A L P Sbjct: 383 QYGLTR-YQGRFYAKAQNIARRARAGYDKALA----DFDLLVMPTVPIIAQPL-PAAGCP 436 Query: 418 VANYLADIF-----TLSTSLAGLPGMSIPCGFGQGEKNGQRPVGLQIIGSYYDEARLLNV 472 +A Y+A F T + G P MSIPCG G PVGL ++G +Y EA L Sbjct: 437 LAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDG-----LPVGLMLVGKHYAEATLYQA 491 Query: 473 AHQYQQVTDW 482 A ++ DW Sbjct: 492 AAAFEASMDW 501 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 164 bits (416), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 164/485 (33%), Positives = 231/485 (47%), Gaps = 45/485 (9%) Query: 5 TIKELSTLLQTKKISAAE-LATLFLDRIGQSDLNAFLHVDRELTLQQAAQADLRIANGDV 63 T+ E+ L ++ S E + L + Q DLN +L D E QA D + +G Sbjct: 7 TLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHG-- 64 Query: 64 TPLTGIPIAHKDLFVTRGWRTTAGSKMLENYVSPFDATVVEQFNAAGTVTLGKLNCDEFA 123 PL+GIPIA KD G +TAG+ L N V ATVVE AG + GK N E A Sbjct: 65 -PLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHELA 123 Query: 124 MGSGNENSYFGAVKNPWDKRAVPGGSSGGSAAAIAARLAPAATGTDTGGSIRQPSALCGV 183 G N N FG +NP+D + GGSSGG A +AARLAPAA GTDTGGS+R P+ALCGV Sbjct: 124 FGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGV 183 Query: 184 TGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMSGFDQRDSTSIERPKEDF 243 G++PT S++G++ + + D GP+A++ D LL M+G RDS + Sbjct: 184 VGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG--SRDSAPL------- 234 Query: 244 TRSLNDSIKGLRIGVPKEFFGAGLAADVEQAVRTALAEYEKLGATLVDISLPK-TELSIP 302 L LR+GV + GL +VE AL + + G LVD+ LP LS Sbjct: 235 ---LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPLSHA 291 Query: 303 VYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYQKTRAEGFGDEVKRRILVGAYVLS 362 + I E +L + +A+D + D+ ++ AE +V R I L Sbjct: 292 AGFAIVLHEFLIDLPAY------LQASDAR---DVIKRVLAEVGSPDVARII---QATLD 339 Query: 363 HGYYDAYYLQAQKIRRLIAQDFQNALTGPDRKCDVIMGPVSPTVAWDLGDKTDDPVANYL 422 + Y A + RR + +++ + D ++ P + A LG+ DD VA Sbjct: 340 QPVPEHAYRLALEQRRTMQALYRDCFRS--QGVDALIFPTTALTARPLGE--DDTVALRG 395 Query: 423 ADIFTLS--------TSLAGLPGMSIPCGFGQGEKNGQRPVGLQIIGSYYDEARLLNVAH 474 + T S+AGLP +SIP G + PVGL++ G + RLL VA Sbjct: 396 ERVPTFPAFARNADGASIAGLPSISIPVGLSEYGL----PVGLELDGPEGSDRRLLQVAA 451 Query: 475 QYQQV 479 + V Sbjct: 452 SLETV 456 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 129/426 (30%), Positives = 198/426 (46%), Gaps = 53/426 (12%) Query: 56 LRIANGDVTPLTGIPIAHKDLFVTRGWRTTAGSKMLENY-VSPFDATVVEQFNAAGTVTL 114 LR A +PL G+P++ KDLF G T AGS +L V+ A VV+ AG + + Sbjct: 59 LRAARLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILI 118 Query: 115 GKLNCDEFAMGSGNENSYFGAVKNPWDKRA----VPGGSSGGSAAAIAARLAPAATGTDT 170 GK N EFA N ++G +N W++ +PGGSS G+A ++ +A AA G+DT Sbjct: 119 GKTNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDT 178 Query: 171 GGSIRQPSALCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMSGFDQ 230 GGS+R PSAL G+TG KPT VS G++ ++ LD GP+A + + C ++ ++G Sbjct: 179 GGSVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG--- 235 Query: 231 RDSTSIERPKEDFTRSLNDSIKGLRIGVPKEFFGAGLAADVEQAVRTALAEYEKLGATLV 290 ED+ L ++GLR+ P G+ V QA RTAL + GA +V Sbjct: 236 ----------EDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIV 285 Query: 291 DISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYQKTRAEGFGDEV 350 E +P + +A A + + + HRA ++ A G+ V Sbjct: 286 -------EAEVPAFNALAAINAKGGFTAAEAYAW-HRALIAEN---------AAGYDQRV 328 Query: 351 KRRILVGAYVLSHGYYDAYYLQAQKIRRLIAQDFQNALTGPDRKCDVIMGPVSPTVAWDL 410 RIL G + + D + + + I ++ AQ L G D ++ P P VA + Sbjct: 329 VSRILRGKDMSAADLLDVLHARPRWIAQVEAQ-----LAG----YDALVMPTVPIVAPRI 379 Query: 411 GD--KTDDPVANYLADIFTLSTSLAGLPG--MSIPCGFGQGEKNGQRPVGLQIIGSYYDE 466 + +DD + T + L G +S+PC G PVGL + + Sbjct: 380 AELQASDDAYYGANGKMLRNPTLINFLDGCALSLPC-----HAPGSAPVGLSLAACGGQD 434 Query: 467 ARLLNV 472 RLL++ Sbjct: 435 KRLLSI 440 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 142/497 (28%), Positives = 222/497 (44%), Gaps = 53/497 (10%) Query: 9 LSTLLQTKKISAAELATLFLDRIGQSD--LNAFLHV-DRELTLQQAAQADLRIANGD-VT 64 LS +++K++S E+ +L+ I + + +NA + + E L +A +AD +A G + Sbjct: 12 LSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRADQLLAQGRYLG 71 Query: 65 PLTGIPIAHKDLFVTRGWRTTAGSKMLENYVSPFDATVVEQFNAAGTVTLGKLNCDEFAM 124 + G+P A KDL +G T+ GS + DAT V + AG + +GK N EF + Sbjct: 72 WMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIFIGKTNVSEFGL 131 Query: 125 GSGNENSYFGAVKNPWDKRAVPGGSSGGSAAAIAARLAPAATGTDTGGSIRQPSALCGVT 184 GS + N FG +N +D + GGSSGG+ AA+A R+ P A G+D GS+R P+A V Sbjct: 132 GSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGSLRNPAAFNNVY 191 Query: 185 GIKPTYGSVSR-YGMIAFASSLDQGGPIAKTAEDCGLLLNAMSGFDQRDSTSIERPKEDF 243 G +PT G V G F L GP+ +T D L +G+D + S + Sbjct: 192 GFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQCPLSSDTDVTGD 251 Query: 244 TRSLNDSIKGLRIGVPKEFFG-AGLAADVEQAVRTALAEYEKLGATLVDISLP--KTELS 300 L KG RIG + G + +V AL+++ +G T V+ P E Sbjct: 252 AAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFADIGCT-VEQCRPDYPMEKL 310 Query: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYQKTRAEGFGDEVKRRILV--GA 358 + V NLS + F D +R +L Sbjct: 311 WETWLVHRQWLVGGNLSVY--------------------------FADPARRGLLKPEAQ 344 Query: 359 YVLSHGYYDAYYLQAQKIRR--LIAQDFQNALTGPDRKCDVIMGPVSPTVAWDLGDKTDD 416 + + G YL A + R + D+ A+ + D ++ P + +D+ K Sbjct: 345 WEVESG----QYLTAADVFRASMARSDWYRAIHKLFEQFDYLLLPSAQMFPFDVATKWPR 400 Query: 417 PVANYLADIF------TLSTSLAGLPGMSIPCGFGQGEKNGQRPVGLQIIGSYYDEARLL 470 VA D + + +LAGLP MS+P GFG NG P+G+QIIG D+ +L Sbjct: 401 NVAGREMDTYHRWMEVVIGPTLAGLPAMSVPVGFG---ANGL-PMGMQIIGPSRDDRGVL 456 Query: 471 NVAHQYQQVTDWHLRAP 487 +A+ + + W +AP Sbjct: 457 QLAYALEGLRPWSAQAP 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,482,871 Number of extensions: 62941 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 11 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)