BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0160.p (394 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 766 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 242 3e-65 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 209 3e-55 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 150 2e-37 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 105 5e-24 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 766 bits (1977), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/394 (92%), Positives = 384/394 (97%) Query: 1 MIHLPGLTFDHGEDIAALRESVAAFAQAEIAPRAAEIDRSDQFPMDLWKKMGDLGVLGIT 60 MIHLPGL+FDHGEDIAALRE+VAAFA +EIAPRAAEIDRSDQFPMDLWKK+GDLGVLGIT Sbjct: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 Query: 61 ADEEYGGAAMGYLAHIVALEEISRASASVGLSYGAHSNLCVNQIKRNGSTEQKNKYLPKL 120 EEYGGA +GYLAHI+A+EEISRASASVGLSYGAHSNLCVNQIKRNG+ EQK KYLP+L Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL Sbjct: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 Query: 181 EAGARGMTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPAENVLGGIGRGVNV 240 EAGARGMTAFLVEKG+KGFSVAQKLDKLGMRGSHTGELVFQDCEVP ENVLGG+GRGVNV Sbjct: 181 EAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 Query: 241 LMSGLDFERTVLSGGPLGIMQACMDVVVPYVHDRKQFGQSIGEFQLMQGKIADMYSTMMA 300 LMSGLDFER+VLSGGPLGIMQACMDVVVPYVHDRKQFGQ+IGEFQLMQGK+ADMYSTMMA Sbjct: 241 LMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMA 300 Query: 301 CKAYVYAVGQACDRAKTPDAVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYP 360 CKAYVYAVGQACDRA + D VRALRKDAAGAILYSAEKATWMAGEAIQ+LGGNGYINEYP Sbjct: 301 CKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYP 360 Query: 361 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETM 394 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFA+T+ Sbjct: 361 VGRLWRDAKLYEIGAGTSEIRRMLIGRELFADTL 394 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 242 bits (618), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 124/372 (33%), Positives = 219/372 (58%), Gaps = 6/372 (1%) Query: 18 LRESVAAFAQAEIAPRAAEIDRSDQFPMDLWKKMGDLGVLGITADEEYGGAAMGYLAHIV 77 +R+++ FAQ + P AA+ DR+ FP + K++ +LG +G+ E++GGA M Y++ ++ Sbjct: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 Query: 78 ALEEISRASASVGLSYGAHSNLCVNQIKRNGSTEQKNKYLPKLISGDFIGALAMSEPNAG 137 ALEEI+ + ++L ++ GS QK ++L L G+ +G ++EP+ G Sbjct: 70 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTG 129 Query: 138 SDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMTAFLVEKGFK 197 SD +++ RA++ GD ++LNG+K +IT+G A V +V+A TD AG +G++ FLV Sbjct: 130 SDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTP 189 Query: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPAENVLGGIGRGVNVLMSGLDFERTVLSGGPL 257 GF V + +K+G S T +++ +C VPA +LG G G + +S L+ R ++ + Sbjct: 190 GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSV 249 Query: 258 GIMQACMDVVVPYVHDRKQFGQSIGEFQLMQGKIADMYSTMMACKAYVYAVGQACDRAKT 317 G+ +A + V Y R+ FG I E Q + ++ADM + + A + V+ Q D+ Sbjct: 250 GMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQLKDQG-- 307 Query: 318 PDAVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYPVGRLWRDAKLYEIGAGT 377 R K+A+ A ++++E A +A +AIQ GG GY +++PV R++RD ++ +I G Sbjct: 308 ----RPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGA 363 Query: 378 SEIRRMLIGREL 389 ++I+R++IGR + Sbjct: 364 NDIQRLVIGRAI 375 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 209 bits (533), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 137/384 (35%), Positives = 207/384 (53%), Gaps = 7/384 (1%) Query: 7 LTFDHGEDIAALRESVAAFAQAEIAPRAAEIDRSDQFPMDLWKKMGDLGVLGITADEEYG 66 + F+ +D A ++S FA E+AP AA+ D FP+D+ K G+LG G+ E G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 67 GAAMGYLAHIVALEEISRASASVGLSYGAHSNLCVNQIKRNGSTEQKNKYLPKLISGDFI 126 G + L V EE++RA S H N+ I G K ++ +L +G I Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIH-NMVSWMIASWGQPSLKAQWCEQLAAGRKI 119 Query: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARG 186 G+ ++EP +GSD S+K A + YVLNGSK +I+ DVLVV A+T E GARG Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGE-GARG 178 Query: 187 MTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPAENVLGGIGRGVNVLMSGLD 246 ++A +V G S +K K+G T + F + +VPA+++LG G+G M GLD Sbjct: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 Query: 247 FERTVLSGGPLGIMQACMDVVVPYVHDRKQFGQSIGEFQLMQGKIADMYSTMMACKAYVY 306 R ++ +G QA +D Y+ +RKQFG+ I +FQ +Q K+ADM + ++A + V Sbjct: 239 GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVR 298 Query: 307 AVGQACDRAKTPDAVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYPVGRLWR 366 D A +P+A A A + + + EA+Q GG GYI EYP+ R +R Sbjct: 299 LAATKLD-AGSPEATTY----CAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFR 353 Query: 367 DAKLYEIGAGTSEIRRMLIGRELF 390 D ++++I GT+EI R++I R+L Sbjct: 354 DVRVHQILEGTNEIMRVIISRKLL 377 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 119/371 (32%), Positives = 193/371 (52%), Gaps = 15/371 (4%) Query: 16 AALRESVAAFAQAEIAPRAAEIDRSDQFPMDLWKKMGDLGVLGITADEEYGGAAMGYLAH 75 A LR++V + +ID + +P + G L +EYGG+ +G Sbjct: 11 ADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEA 70 Query: 76 IVALEEISRASASVGLSYGAHSNLCVNQIKRNGSTEQKNKYLPKLISGDF-IGALAMSEP 134 V +EEI+R + G +G N+ + R+GS EQK +YLP++ SG+ + ++A++EP Sbjct: 71 SVIMEEINRTGGNSGACHGQMYNM--GTLLRHGSPEQKERYLPRIASGELRLQSMAVTEP 128 Query: 135 NAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTD-LEAGAR---GMTAF 190 G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T LE +R GM+ F Sbjct: 129 TTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIF 188 Query: 191 LVE-KGFKGFSVAQKLDKLGMRGSHTGELVFQDCEVPAENVLGGIGRGVNVLMSGLDFER 249 LV+ K G + + L M T EL F + E+PAEN++G GRG ++ GL+ ER Sbjct: 189 LVDLKEAIGHGMEVR-PILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAER 247 Query: 250 TVLSGGPLGIMQACMDVVVPYVHDRKQFGQSIGEFQLMQGKIADMYSTMMACKAYVYAVG 309 T+++ +G +D Y +R F + IG Q +Q +A+ Y + A Y Sbjct: 248 TLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRY--- 304 Query: 310 QACDRAKTPDAVRALRKDAAGAILYSAEKATWMAGEAIQALGGNGYINEYPVGRLWRDAK 369 QAC DA ++ A A +A+ A A +Q GG G+ EY V R +R+ + Sbjct: 305 QAC---ALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETR 361 Query: 370 LYEIGAGTSEI 380 LY++ ++ + Sbjct: 362 LYQVAPISTNL 372 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 96/402 (23%), Positives = 179/402 (44%), Gaps = 37/402 (9%) Query: 7 LTFDHGEDIAALRESVAAFAQ---AEIAPRAAEIDRSDQ---FPMDLWKKMGDLGVLGIT 60 + F E AL+ AFA ++ P + + D+ ++ M D G + Sbjct: 3 IDFQLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIKAL 62 Query: 61 ADEEYGGAAMGYLAHIVALEEISRA-----SASVGLSYGAHSNLCVNQIKRNGSTEQKNK 115 E +GG A+ L +A EE++ SA +G G H+ + +G+ EQK + Sbjct: 63 LPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHA------VVHHGTPEQKQR 116 Query: 116 YLPKLIS-GDFIGALAMSEPNAGSDV------VSMKLRADKKGDRYVLNGSKMWITNGPD 168 +LP I G + ALA +E G++ V ++ A +GD +V+NG+K + TNG Sbjct: 117 FLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTG 176 Query: 169 AD-----VLVVYAKTDLE-AGARGMTAFLVEKGFKGFSVAQKLDKLGMRGSHTGELVFQD 222 D + V +TD + + +V G + LD +G R + + + F + Sbjct: 177 WDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISPRVHFNN 236 Query: 223 CEVPAENVLGGIGRGVNVLMSGLDFERTVLSGGPLGIMQACMDVVVPYVH-DRKQFGQSI 281 VPA N++G G G+++L + + ++ +G+M+ D+ + + DR+ + Sbjct: 237 VRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPV 296 Query: 282 GEFQLMQGKIADMYSTMMACKAYVYAVGQACDRAKTPDAVRALRKDAAGAILYSAEKATW 341 E Q + +AD+ M +A Y ++C + + R+ A Y +E Sbjct: 297 IEHQNVGFMLADI---KMRIEAARYLTWKSCHQLDLTEGRS--RELAIITKTYCSELCVQ 351 Query: 342 MAGEAIQALGGNGYINEYPVGRLWRDAKLYEI-GAGTSEIRR 382 + +A++ +G + Y + YP+ L +DA + + G +RR Sbjct: 352 VVYDAMRVVGVDAYTDLYPLAGLMQDALCFPLYDGGNMGVRR 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,293,299 Number of extensions: 56485 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 12 Length of query: 394 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 307 Effective length of database: 1,207,925 Effective search space: 370832975 Effective search space used: 370832975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)