BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0159.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [H... 254 5e-69 gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [H... 172 4e-44 gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herba... 104 1e-23 gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [H... 97 1e-21 gi|300313295|ref|YP_003777387.1| acetyl-CoA acetyltransferase [H... 92 5e-20 >gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 254 bits (650), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 128/212 (60%), Positives = 156/212 (73%), Gaps = 6/212 (2%) Query: 10 EDPIVIVGAARTPMGAFQGELSALTAGDLGAAAIKAALERAGVAPDQV---QDVLFGNCL 66 EDP+VIV AART MG+FQG L+ L A LGA AI AAL+RA ++ Q+ + V G L Sbjct: 3 EDPVVIVSAARTAMGSFQGALADLPAPHLGAVAISAALQRAQLSAAQIAQIEQVWMGCVL 62 Query: 67 MAGQGQAPARQAAIKAGIPVSAGAVTLSKMCGSAMQATMFGFDSILTGTNDVVVAGGMES 126 AG GQAPARQAA++AG+P + TL+K+CGSAMQA M G DSIL G+ +VVAGGMES Sbjct: 63 QAGLGQAPARQAALRAGLPQTVACATLNKVCGSAMQAVMLGHDSILAGSASMVVAGGMES 122 Query: 127 MTNAPYLIPKARGGYRIGHGMIYDHMMLDGLEDAYD---KGRAMGTFGEDCAAKYHFTRE 183 M+NAPYL+PKAR GYR+GH + DHM LDGLEDAY +GR MGTF EDCA+++ FTR Sbjct: 123 MSNAPYLLPKARRGYRLGHDRLLDHMFLDGLEDAYSDQYRGRLMGTFAEDCASEFGFTRA 182 Query: 184 DQDAFAIASVKRAQQATADGSFKWEIAPVTVA 215 QDAFA+ S RA+QA + G F WE+ PV VA Sbjct: 183 QQDAFAVQSTLRARQAISGGGFDWEVVPVDVA 214 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 68/100 (68%), Positives = 80/100 (80%) Query: 223 PIGAINNLYKKIGWSTADVDLFEINEAFAAVPMAAMKELGIPHDKVNIHGGACALGHPIG 282 PIG + ++ K G A DLFEINEAFA VPMAA +EL I +K+N+HGGACALGHPIG Sbjct: 300 PIGVLRKIFAKTGLDAASTDLFEINEAFAVVPMAASRELDIDMEKINVHGGACALGHPIG 359 Query: 283 ASGARIIVTLIGALKKTGGKRGVASLCIGGGEGTALAIEL 322 ASGARIIV+L+GALK G K G+ASLCIGGGE TA+A+EL Sbjct: 360 ASGARIIVSLLGALKARGLKTGIASLCIGGGEATAIALEL 399 >gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 391 Score = 172 bits (436), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 101/271 (37%), Positives = 151/271 (55%), Gaps = 19/271 (7%) Query: 11 DPIVIVGAARTPMGAFQGELSALTAGDLGAAAIKAALERAGVAPDQVQDVLFGNCLMAGQ 70 D +VIV A RT +G F G LS + A DLGA IKA L + G+ P+ + +V+ G L AG Sbjct: 2 DDVVIVAAQRTAIGKFGGALSKIAAADLGAQVIKALLAKTGIKPEAISEVILGQVLTAGL 61 Query: 71 GQAPARQAAIKAGIPVSAGAVTLSKMCGSAMQATMFGFDSILTGTNDVVVAGGMESMTNA 130 GQ PARQ+ IK+G+P A + K+CGS ++A +I G +V+AGG E+M+ + Sbjct: 62 GQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENMSAS 121 Query: 131 PYLIPKARGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGTFGEDCAAKYHFTREDQDAFAI 190 P+++ +R G+R+G + D M++DGL D Y++ MG E+ A K+ +RE+QDAFA Sbjct: 122 PHVLNNSRDGFRMGDAKLTDTMIVDGLWDVYNQYH-MGITAENVAKKFEISREEQDAFAA 180 Query: 191 ASVKRAQQATADGSFKWEIAPVTVATRGPAXV--------------PIGAINNLYKKIGW 236 AS +A+ A G FK EI P+ + + V + + + K G Sbjct: 181 ASQNKAEAAQKAGKFKDEIVPIEIKGKKETVVFDTDEFVKHGVTAESLATLRPAFDKAGT 240 Query: 237 STADVDLFEINEAFAAVPMAAMK---ELGIP 264 TA + IN+ AAV + + K ELG+P Sbjct: 241 VTAG-NASGINDGAAAVVVTSAKLAAELGLP 270 Score = 111 bits (278), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 54/99 (54%), Positives = 71/99 (71%) Query: 223 PIGAINNLYKKIGWSTADVDLFEINEAFAAVPMAAMKELGIPHDKVNIHGGACALGHPIG 282 P+ A KK GW+ D+DL EINEAFAA +A K++G K+N++GGA ALGHPIG Sbjct: 292 PVPASQLTLKKAGWTPQDLDLMEINEAFAAQAIAVNKQMGWDTSKINVNGGAIALGHPIG 351 Query: 283 ASGARIIVTLIGALKKTGGKRGVASLCIGGGEGTALAIE 321 ASG R++VTL+ + + K+G+ASLCIGGG G ALA+E Sbjct: 352 ASGCRVLVTLLHEMVRRDAKKGLASLCIGGGMGVALAVE 390 >gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 74/210 (35%), Positives = 118/210 (56%), Gaps = 7/210 (3%) Query: 14 VIVGAARTPMGAFQGELSALTAGDLGAAAIKAALER-AGVAPDQVQDVLFGNCLMAGQ-G 71 +I A RTP G + G L A+ A DL AA I++ +ER GV +V+D+L+G AG+ Sbjct: 4 LICDAIRTPFGRYGGALGAVRADDLAAAPIRSLMERNPGVDWSRVEDILYGCANQAGEDN 63 Query: 72 QAPARQAAIKAGIPVSAGAVTLSKMCGSAMQATMFGFDSILTGTNDVVVAGGMESMTNAP 131 + AR A + AG+P++ T++++CGS++ A +I +G +++AGG+ESMT AP Sbjct: 64 RNVARMAGLLAGLPIAVPGSTVNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMTRAP 123 Query: 132 YLIPKARGGYRIGHGMIYDHMM----LDGLEDAYDKGRAMGTFGEDCAAKYHFTREDQDA 187 +++ KA + I+D + ++ L A +M E+ A + R DQDA Sbjct: 124 FVMGKAESAF-ARSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDFQINRADQDA 182 Query: 188 FAIASVKRAQQATADGSFKWEIAPVTVATR 217 FA+ S +R A A G F EIAP+T+ + Sbjct: 183 FALRSQQRWAAAQAAGFFAGEIAPLTIPQK 212 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 47/101 (46%), Positives = 72/101 (71%), Gaps = 2/101 (1%) Query: 223 PIGAINNLYKKIGWSTADVDLFEINEAFAAVPMAAMKELGIPHD--KVNIHGGACALGHP 280 P A+ + ++G + A +D+ E+NEAFAA +A M++LG+P D VN +GGA A+GHP Sbjct: 298 PAPAVRKVLAQVGLTLAQMDVIELNEAFAAQGLAVMRDLGLPDDAAHVNPNGGAIAIGHP 357 Query: 281 IGASGARIIVTLIGALKKTGGKRGVASLCIGGGEGTALAIE 321 +GASGAR++ T I L+++GG+ + ++CIG G+G AL IE Sbjct: 358 LGASGARLVTTAINQLERSGGRYALCTMCIGVGQGIALVIE 398 >gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 70/211 (33%), Positives = 110/211 (52%), Gaps = 7/211 (3%) Query: 13 IVIVGAARTPMGAFQGELSALTAGDLGAAAIKAALER-AGVAPDQVQDVLFGNCLMAGQ- 70 + I A RTP+G + G L + A DLGA ++A +ER V V DV++G AG+ Sbjct: 4 VFICDAIRTPIGRYGGALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQAGED 63 Query: 71 GQAPARQAAIKAGIPVSAGAVTLSKMCGSAMQATMFGFDSILTGTNDVVVAGGMESMTNA 130 + AR + + AG+P T++++CGS M A +I +G +++AGG+ESMT A Sbjct: 64 NRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVESMTRA 123 Query: 131 PYLIPKARGGYRIGHGMIYDHMMLDGLEDAYDKGR----AMGTFGEDCAAKYHFTREDQD 186 P+++ KA + I D + +A K + AM E+ A + +R DQD Sbjct: 124 PFVMGKADSAFS-RQAAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRADQD 182 Query: 187 AFAIASVKRAQQATADGSFKWEIAPVTVATR 217 FA+ S +A A A+G+ EI PV + + Sbjct: 183 QFAVRSQAKAAAAQANGTLAQEIVPVVIPQK 213 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 50/134 (37%), Positives = 80/134 (59%), Gaps = 11/134 (8%) Query: 199 ATADGSFKWEIAPVT---------VATRGPAXVPIGAINNLYKKIGWSTADVDLFEINEA 249 A+A+ K+++ P VA R P A + ++G + +D+ E+NEA Sbjct: 265 ASAEAVEKYQLKPRARILGMATAGVAPRIMGMGPAPASKKVLAQLGMTIDQMDVIELNEA 324 Query: 250 FAAVPMAAMKELGIPHD--KVNIHGGACALGHPIGASGARIIVTLIGALKKTGGKRGVAS 307 FA+ +A ++ELG+ D +VN +GGA ALGHP+G SGAR++ T L++TGG+ + + Sbjct: 325 FASQGLAVLRELGVADDDARVNPNGGAIALGHPLGMSGARLVTTATYQLERTGGRYALCT 384 Query: 308 LCIGGGEGTALAIE 321 +CIG G+G A+ IE Sbjct: 385 MCIGVGQGIAMVIE 398 >gi|300313295|ref|YP_003777387.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 92.0 bits (227), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 45/99 (45%), Positives = 66/99 (66%) Query: 223 PIGAINNLYKKIGWSTADVDLFEINEAFAAVPMAAMKELGIPHDKVNIHGGACALGHPIG 282 P+ AI L ++ G + AD+ FEINEA A +A +ELGI +++N++GGA ALGHP+ Sbjct: 313 PVPAIRTLLERNGLTVADIGCFEINEAQGAQIVAVERELGIDRERLNVNGGAIALGHPLA 372 Query: 283 ASGARIIVTLIGALKKTGGKRGVASLCIGGGEGTALAIE 321 A+G R+ +T + + G + VAS CIGGG+G AL +E Sbjct: 373 ATGVRLSITAAREMNRRGARYCVASACIGGGQGIALLLE 411 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 95/206 (46%), Gaps = 4/206 (1%) Query: 13 IVIVGAARTPMGAFQGELSALTAGDLGAAAIKAALERAGVAPDQVQDVLFGNCLM--AGQ 70 I +VG RTPM + S L+ D+G + L R G+ P + V+ GN A Q Sbjct: 13 IWLVGGVRTPMVDYCTSFSQLSPTDMGIKVAREVLARTGIDPADIGSVVAGNMAPGDAYQ 72 Query: 71 GQAPARQAAIKAGIPVSAGAVTLSKMCGSAMQATMFGFDSILTGTNDVVVAGGMESMTNA 130 P R + AG+P+S A+ + ++CG+ + D I G + + G ESMT Sbjct: 73 YMLP-RHIGLYAGVPISTPAIMVQRICGTGFELIRQAGDQIERGYTEAALIVGSESMTRN 131 Query: 131 PYLIPKARGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGTFGEDCAAKYHFTREDQDAFAI 190 P R G+++G + + M + L D G M E+ A KY TR + D +A Sbjct: 132 PIAAFGHRSGFKLGAPVEFQDYMWEALVDP-APGITMPQTAENLAKKYGITRAEVDEYAA 190 Query: 191 ASVKRAQQATADGSFKWEIAPVTVAT 216 S +RA +A A G EI PV T Sbjct: 191 YSFERALKAQAAGFHAGEIVPVVNET 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 963,458 Number of extensions: 39249 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 12 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)